This book is the compendium of Chinese meteorologist Song Yingjie's research on the 24 solar terms.It encompasses the author's years of accumulated research,data,and cultural knowledge.From a meteorological pe...This book is the compendium of Chinese meteorologist Song Yingjie's research on the 24 solar terms.It encompasses the author's years of accumulated research,data,and cultural knowledge.From a meteorological perspective,this book applies charts based on climate big data,combined with literary writing techniques and meteorological algorithms,to present the knowledge of the twenty-four solar terms to readers.展开更多
Triple-negative breast cancer(TNBC)is the most malignant subtype of breast cancer that lacks reliable targets for diagnosis and therapy.Non-coding RNA(ncRNA)-encoded products hold promise for addressing this unmet nee...Triple-negative breast cancer(TNBC)is the most malignant subtype of breast cancer that lacks reliable targets for diagnosis and therapy.Non-coding RNA(ncRNA)-encoded products hold promise for addressing this unmet need.By analyzing the reported ribosomal RNA sequencing data,combined with the TCGA,ORFfinder,SmProt databases,we identified CDKN2B-AS1,a TNBC-upregulated lncRNA encoding a 66-amino-acid peptide via CUG-initiated translation.CRISPR-Cas9 gene editing and mass spectrometry confirmed endogenous expression of this peptide,designated 66CTG,in TNBC cells.Functionally independently of its host RNA,66CTG promoted the proliferation of TNBC cells and the tumor growth of TNBC xenograft by stabilizing c-Myc protein and enhancing Cyclin D1 transcription.Immunohistochemistry of 89 clinical TNBC paraffin samples revealed positive correlations among 66CTG,c-Myc,and Cyclin D1 expression levels.展开更多
文摘This book is the compendium of Chinese meteorologist Song Yingjie's research on the 24 solar terms.It encompasses the author's years of accumulated research,data,and cultural knowledge.From a meteorological perspective,this book applies charts based on climate big data,combined with literary writing techniques and meteorological algorithms,to present the knowledge of the twenty-four solar terms to readers.
基金supported by National Key Research and Development Program of China(2023YFA1800500,2023ZD0502200,SQ2023AAA030478,2020YFA0112300)National Natural Science Foundation of China(82203413,U2102203,82430084,82173014,82472806)+5 种基金Biomedical Projects of Yunnan Key Science and Technology Program(202302AA310046)Yunnan Fundamental Research Projects(Major project:202501AS070023,202201BC070002,General project:202401AT070171,202201AT070290)Yunnan Academician Expert Workstation(202505AF350058)Yunnan Revitalization Talent Support Program(Yunling Shcolar)Yunnan Revitalization Talent Support Program,Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University(202401AY070001-026)The Innovative Research Team of Yunnan Province(202405AS350016).
文摘Triple-negative breast cancer(TNBC)is the most malignant subtype of breast cancer that lacks reliable targets for diagnosis and therapy.Non-coding RNA(ncRNA)-encoded products hold promise for addressing this unmet need.By analyzing the reported ribosomal RNA sequencing data,combined with the TCGA,ORFfinder,SmProt databases,we identified CDKN2B-AS1,a TNBC-upregulated lncRNA encoding a 66-amino-acid peptide via CUG-initiated translation.CRISPR-Cas9 gene editing and mass spectrometry confirmed endogenous expression of this peptide,designated 66CTG,in TNBC cells.Functionally independently of its host RNA,66CTG promoted the proliferation of TNBC cells and the tumor growth of TNBC xenograft by stabilizing c-Myc protein and enhancing Cyclin D1 transcription.Immunohistochemistry of 89 clinical TNBC paraffin samples revealed positive correlations among 66CTG,c-Myc,and Cyclin D1 expression levels.
