This work demonstrated quantitatively that DSA （dansylamide） and apo CA （dezincified carbon anhydrase） interacted with each other in the presence of Zn2＋ （zinc ion）. The fluorescence emission of DSA in the pres...This work demonstrated quantitatively that DSA （dansylamide） and apo CA （dezincified carbon anhydrase） interacted with each other in the presence of Zn2＋ （zinc ion）. The fluorescence emission of DSA in the presence of Zn2＋ and the intensity of fluorescence, which was proportional to the concentration of Zn2＋, could be used in the measurement of Zn2＋ concentration. Considering that Zn2＋ was the active center of enzymes like CA （carbon anhydrase）, two dezincification reagents were compared to ensure the validity of our method. A certain range concentration of Zn2＋ could be measured by the reaction product of apo CA, DSA and Zn2＋, where the measurement limitation was about 60 nmol/L. Interaction of CA with DSA was also studied with infrared spectrometry.展开更多
基金Supported by National Natural Science Foundation of China(NSFC)(No.61177076)the Fundamental Research Funds for the Central Universities of WUT(Nos.2012-Ia-020 and 2013-Ia-038)
文摘This work demonstrated quantitatively that DSA （dansylamide） and apo CA （dezincified carbon anhydrase） interacted with each other in the presence of Zn2＋ （zinc ion）. The fluorescence emission of DSA in the presence of Zn2＋ and the intensity of fluorescence, which was proportional to the concentration of Zn2＋, could be used in the measurement of Zn2＋ concentration. Considering that Zn2＋ was the active center of enzymes like CA （carbon anhydrase）, two dezincification reagents were compared to ensure the validity of our method. A certain range concentration of Zn2＋ could be measured by the reaction product of apo CA, DSA and Zn2＋, where the measurement limitation was about 60 nmol/L. Interaction of CA with DSA was also studied with infrared spectrometry.
