Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the...Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the effects of dandelions,both alone and combined with Lactobacillus plantarum and Lactobacillus buchneri(LAB)on fermentation quality,bacteriome and mycobiome after 180 d of anaerobic and 4 d of aerobic fermentation of whole-plant corn.In vitro dry matter digestion(IVDMD)and gas production from anaerobically fermented whole-plant corn were also assessed.The results demonstrated that dandelions,either alone or combined with LAB,effectively improved fermentation quality by reducing NH_(3)-N concentrations(22.72–25.99%)after anaerobic fermentation,decreasing the proliferation of yeast and molds to enhance the aerobic stability.Notably,the changes in the bacteriome were more pronounced than those in the mycobiome after aerobic exposure.The addition of dandelions or the combination reduced Acetobacter fabarum abundance,a member of the Acetobacter that was spoilage-induced microbe indicated by correlation analysis.Besides,these treatments facilitated competition relations of microbiome which contributed to the enhanced aerobic stability.Furthermore,dandelions reduced CH_(4)and CO_(2)emissions by 14.88 and 13.73%,respectively,and also positively influencing IVDMD by 4.46%.Collectively,dandelion alone or combined with LAB are promising strategies to improve the aerobic stability of anaerobically fermented whole-plant corn,a process linked to the interactions between the bacteriome and mycobiome,and to contribute to clean production by reducing rumen CH_(4)and CO_(2)emissions.展开更多
As a leafy vegetable,dandelion(Taraxacum antungense Kitag.)leaves are subject to senescence,such as yellowing and even decay,during postharvest storage.Calcium chloride(CaCl_(2))andε-polylysine(ε-PL)treatments can s...As a leafy vegetable,dandelion(Taraxacum antungense Kitag.)leaves are subject to senescence,such as yellowing and even decay,during postharvest storage.Calcium chloride(CaCl_(2))andε-polylysine(ε-PL)treatments can safely and effectively improve the postharvest quality of fruits and vegetables.To improve the storage quality,dandelions were treated with 0.5%(mass concentration)CaCl_(2)and 0.9 g/Lε-PL and stored at 4±0.5℃.The results showed that the combination treatment of CaCl_(2)andε-PL(CaCl_(2)+ε-PL treatment)could effectively delay the yellowing and chlorophyll content decline,as well as reduce the browning index,when compared with those of the control and single treatments.The CaCl_(2)+ε-PL treatment also enhanced the synthesis and accumulation of secondary metabolites.The combination treatment could significantly enhance the enzyme activities of phenylalanine ammonia-lyase(PAL),4-coumarate-Co A ligase(4CL),and cinnamate-4-hydroxylase(C4H)in phenylpropanoid metabolism and upregulate the expression levels of Ta PAL,Ta4CL,and Ta C4H.Meanwhile,ε-PL treatment was more effective than CaCl_(2)treatment in promoting the expression of these genes.Correlation analysis indicates that the transcription levels and activities of the three enzymes,as well as the contents of secondary metabolites,are linked to the quality changes of dandelions treated with CaCl_(2)+ε-PL during cold storage.Principal component analysis was used to assess the storage quality of dandelions during cold storage,and the results showed the following rankings:CaCl_(2)+ε-PL>ε-PL>CaCl_(2)>control.These findings suggest that CaCl_(2)+ε-PL treatment can improve the postharvest storage quality of dandelions by modulating the phenylpropane metabolic pathway.展开更多
In this paper,the effect of the morphological profile of dandelion seed on flight lift force under crosswind conditions is explored.Existing studies primarily focus on the flight characteristics of dandelion seed duri...In this paper,the effect of the morphological profile of dandelion seed on flight lift force under crosswind conditions is explored.Existing studies primarily focus on the flight characteristics of dandelion seed during its fall,emphasizing the influence of the complex filament structure on the formation of wake vortices.However,research on the flight lift force due to the dandelion seed’s morphological profile under lateral crosswind conditions is quite limited.This study investigates the aerodynamic behavior of dandelion seed using a novel virtual barrier model.This model is proposed,based on the regular pattern of the filaments’outer contours and the virtual barrier effect produced by their columnar array.Through elaborate numerical simulations,it is found that the morphological profile of dandelion seed possesses superior aerodynamic properties,particularly in generating lift force under crosswind conditions.This characteristic is a crucial mechanism for the long-distance dispersal of dandelion seed.Subsequently,the study extends to examine the aerodynamic performance of the model at varying degrees of opening angles and inflow attack angles,offering a fresh perspective on understanding the flight characteristics of dandelion seed in natural environments.The findings not only contribute to the field of plant aerodynamics but also provide insights into potential biomimetic applications in engineering.展开更多
The thermal deformation behavior of FV520B stainless steel is investigated.Isothermal compression tests were conducted at temperatures ranging from 600 to 900℃ and strain rates from 0.001 to 10 s^(−1).The true stress...The thermal deformation behavior of FV520B stainless steel is investigated.Isothermal compression tests were conducted at temperatures ranging from 600 to 900℃ and strain rates from 0.001 to 10 s^(−1).The true stress–strain curves were corrected for friction and temperature due to the drum shape and adiabatic heating.The comparison shows that there is a large difference between the stress before and after the correction,which proves that the correction is necessary.Five constitutive models were developed:the original Arrhenius model,the strain correction Arrhenius model,a new modified Arrhenius model,the back propagation neural network model(BPNN)and the dandelion optimization BPNN model(DO-BPNN).The DO-BPNN model showed the highest prediction accuracy though it was more computationally intensive than the other models.The new modified Arrhenius model performed a better predictive capacity than the strain correction version,while it showed a negligible increase in the number of parameters and computational time.Although artificial neural network-based models exhibit superior accuracy compared to the Arrhenius models,their application in finite element simulations still faces notable challenges.展开更多
To optimize the operating efficiency and extend the lifespan of the multistack fuel cell hybrid system(MFCHS),this paper proposes a two-layer multiobjective optimal energy management strategy that considers the degrad...To optimize the operating efficiency and extend the lifespan of the multistack fuel cell hybrid system(MFCHS),this paper proposes a two-layer multiobjective optimal energy management strategy that considers the degradation of the fuel cell and the battery.Regarding the issues that power fluctuations damage the fuel cells'lifespan and high-current charging and discharging lead to battery capacity decay,the first layer of the strategy adopts locally weighted scatterplot smoothing(LOWESS)to smooth the output power of the fuel cells and prevent the battery from operating under high-current conditions.The second layer considers the uneven degree of degradation among the fuel cells and employs the dandelion optimizer(DO)algorithm to solve the objective function with an aging adaptive factor,optimizing the efficiency and lifespan.Meanwhile,the DO algorithm is enhanced by tent chaotic mapping and differential variation to improve the convergence speed and accuracy.Compared with the equivalent hydrogen consumption minimization strategy(ECMS)and the equal distribution strategy,the proposed strategy improves the average operating efficiency of the fuel cells,effectively reduces the degradation of the fuel cells and the capacity degradation of the battery,and maintains the performance consistency among the fuel cells.展开更多
We investigated the liver protective activity of dandelion polyphenols(DP)against acetaminophen(APAP;Paracetamol)-induced hepatotoxicity.Mice were acclimated for 1 week and randomly divided into the following groups(n...We investigated the liver protective activity of dandelion polyphenols(DP)against acetaminophen(APAP;Paracetamol)-induced hepatotoxicity.Mice were acclimated for 1 week and randomly divided into the following groups(n=9 per group):Control,APAP,APAP+DP(100 mg·kg^–1),APAP+DP(200 mg·kg^–1),and APAP+DP(400 mg·kg^–1)groups.Mice were pretreated with DP(100,200,and 400 mg·kg^–1)by oral gavage for 7 d before being treated with 350 mg·kg^–1 APAP for 24 h to induced hepatotoxicity.Severe liver injury was observed,and hepatotoxicity was analyzed after 24 h by evaluation of biochemical markers,protein expressions levels,and liver histopathology.Pretreatment with DP was able to restore serum liver characteristics(aspartate transaminase,AST;alanine aminotransferase,ALT;alkaline phosphatase,AKP),improve redox imbalance(superoxide dismutase,SOD;glutathione,GSH;malondialdehyde,MDA),and decrease inflammatory factors(tumor necrosis factor-α,TNF-α;interleukin-1β,IL-1β).Pretreatment with DP also significantly inhibited the expression levels of nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2).Furthermore,DP pretreatment could inhibit the apoptosis of liver cells caused by APAP through up-regulation of Bcl-2 and down-regulation of Bax and caspase-9 protein.DP also down-regulated p-JNK protein expression levels to inhibit APAP-induced mitochondrial oxidative stress and up-regulated the expression of Nrf-2 and its target gene HO-1.The histopathological staining demonstrated that DP pretreatment could inhibit APAP-induced hepatocyte infiltration,congestion,and necrosis.Our results demonstrate that DP pretreatment could protect against APAP-induced hepatic injury by activating the Nrf-2/HO-1 pathway and inhibition of the intrinsic apoptosis pathway.展开更多
Plants of the genus Taraxacum, commonly known as dandelions, are used to treat breast cancer in traditional folk medicine. However, their use has mainly been based on empirical findings without sufficient scientific e...Plants of the genus Taraxacum, commonly known as dandelions, are used to treat breast cancer in traditional folk medicine. However, their use has mainly been based on empirical findings without sufficient scientific evidence. Therefore, we hypothesized that dandelions would behave as a Selective estrogen receptor modulator （SERM） and be effective as hormone replacement therapy （HRT） in the postmenopausal women. In the present study, in vitro assay systems, including cell proliferation assay, reporter gene assay, and RT-PCR to evaluate the mRNA expression of estrogen-related genes （pS2 and progesterone receptor, PR）, were performed in human breast cancer cells. Dandelion ethanol extract （DEE） significantly increased cell proliferation and estrogen response element （ERE）-driven luciferase activity. DEE significantly induced the expression of estrogen related genes such as pS2 and PR, which was inhibited by tamoxifen at 1 gmol L-1. These results indicated that DEE could induce estrogenic activities mediated by a classical estrogen receptor pathway. In addition, immature rat uterotrophic assay was carried out to identify estrogenic activity of DEE in vivo. The lowest concentration of DEE slightly increased the uterine wet weight, but there was no significant effect with the highest concentration of DEE. The results demonstrate the potential estrogenic activities of DEE, providing scientific evidence supporting their use in traditional medicine.展开更多
The protection of the liver as an essential organ in the body against oxidative stress and deleterious compounds has been the subject of recent investigations.Among different compounds,medicinal plants play an importa...The protection of the liver as an essential organ in the body against oxidative stress and deleterious compounds has been the subject of recent investigations.Among different compounds,medicinal plants play an important role due to their hepatoprotective effects.Taraxacum officinale or"common dandelion"is a popular plant that has been traditionally used for its hepatoprotective effects.Currently,there are limited clinical studies on its hepatoprotective effects.The aim of this review article is to evaluate the hepatoprotective effects of dandelion and its mechanism of action.We reviewed literature up to July 2019 on"Taraxacum officinale"or"dandelion"and hepatoprotection.Currently available pharmacological studies indicate that dandelion extracts have hepatoprotective effects against chemical agents due to its antioxidant and antiinflammatory activities.The anti-inflammatory effects of dandelion,the prebiotic effects of its oligofructans,inhibitory effects against the release of lipopolysaccharides and fasting induced adipose factor,digestive enzymes,and enhancing effects of lipogenesis,reduce lipid accumulation and liver inflammation,which directly or indirectly improve the liver functions.Given emerging evidence on hepatoprotective effects of dandelion,designing large human clinical studies is essential.展开更多
The paper was to study DPPH （1,1-diphenyl-2-trinitrobenzene hydrazine） scavenging ability of different concentrations of tlavonoid and polysaccheride from dandelion. Flavonoids were extracted from dandelion using al...The paper was to study DPPH （1,1-diphenyl-2-trinitrobenzene hydrazine） scavenging ability of different concentrations of tlavonoid and polysaccheride from dandelion. Flavonoids were extracted from dandelion using alcohol precipitation ultrasonic extraction method and polysaccharide was extracted using water ul- trasonic extraction method. DPPH free radical scavenging ability of flavonoid and polysaccharide was determined using ultravialet spectrophotometer method. The difference in DPPH free radical scavenging ability between flavonoids and polysacchafide was analyzed statistically. The results showed that DPPH scavenging ability was the best when the concentration of flavanoid was 1.6 mg/mL; the scavenge rate was up to 96.11% and I Cso （median inhibitory concentration of inhibi^rs） was 0.38 mg/mL. DPPH scavenging ability was the best when the concentration of polysaccheride was 1.4 mg/mL; the scavenge rate was 89.33% and IC5o was 0.51 mg/mL. Statistical results showed that there were significant differences in DPPH free radical scavenging ability between dandelion flavonoid and polysaeoharide （P 〈 0.05 ）. Flavonoid had significantly higher scavenging ability than polysaccharide （P 〈 0.05）. The study lays a theoretical foundation for further research on dan- delion flavonoid, and provides a theoretical basis for development and utilization of dandelion resources.展开更多
A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all...A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all optimization problems. A self-adapting and efficient dandelion algorithm is proposed in this work to lower the number of DA's parameters and simplify DA's structure. Only the normal sowing operator is retained;while the other operators are discarded. An adaptive seeding radius strategy is designed for the core dandelion. The results show that the proposed algorithm achieves better performance on the standard test functions with less time consumption than its competitive peers. In addition, the proposed algorithm is applied to feature selection for credit card fraud detection(CCFD), and the results indicate that it can obtain higher classification and detection performance than the-state-of-the-art methods.展开更多
It is of great significance to study the water absorption and expansion of cells,which are essential for the growth and life regulation of plants,not only for understanding the complex life phenomena of plants,but als...It is of great significance to study the water absorption and expansion of cells,which are essential for the growth and life regulation of plants,not only for understanding the complex life phenomena of plants,but also for the manufacture of biomimetic materials.The main purpose of this study is to shed light on the mechanism of a novel coiling phenomenon of the split dandelion scape soaked in water and the high concentration mannitol solution.We discovered that the difference between water absorption/dehydration capacities of inner cells and lateral cells of the split dandelion scape generates asymmetric expansion/contraction between inner and lateral layers,resulting two opposite coiled patterns.Analogous to the deformable hypothesis of the thermal expansion model,the water absorption-induced cell expansion model was proposed to explain the coiling process of the split dandelion scape.Both the simulation and experimental results revealed that the coiling of dandelion scape is strongly depended on the response of cells to the stimulation of the solution environment.Showing considerable potential load bearing capacity of plants,the coiling phenomenon can be an inspiration to the designs of soft robots and bionic sensors for humidity control.展开更多
This novel study identifi es the effective anti-inflammatory phenolic compounds in dandelion and provides mechanistic insights into their interactions with receptor proteins(toll-like receptor 4,TLR4;co-receptor myelo...This novel study identifi es the effective anti-inflammatory phenolic compounds in dandelion and provides mechanistic insights into their interactions with receptor proteins(toll-like receptor 4,TLR4;co-receptor myeloid differentiation protein-2,MD-2)using UHPLC-ESI-MS/MS,lipopolysaccharide(LPS)-stimulated THP-1 cell line,fluorescence quenching and anisotropy,molecular docking(single ligand and multi-ligand docking)and molecular dynamics simulation.A 50%aqueous methanol extract had a greater anti-inflammatory effect and higher chicoric acid content,compared with the 100%water and 100%methanol extracts.Chicoric acid,chlorogenic acid,methylophiopogonone A,caffeic acid,gallic acid monohydrate and 4’-O-demethylbroussonin A had relatively high binding energies and contents in all extracts.Chicoric acid competed with chlorogenic acid,4’-O-demethylbroussonin A and quercetin for MD-2.Among dandelion’s phenolics,chicoric acid most effectively hindered TLR4-MD-2 complex formation,with a quenching constant of 0.62×10^(6) L/mol for MD-2 or TLR4 at 320 K,and binding energies of-6.87 and-5.97 kcal/mol,respectively,for MD-2 and TLR4.展开更多
As a traditional Chinese herb,dandelion,containing complicated active ingredients which includes polysaccharide,flavonoid,terpene,pigment,phytosterol,coumarin,organic acid and etc,plays significant roles in various ph...As a traditional Chinese herb,dandelion,containing complicated active ingredients which includes polysaccharide,flavonoid,terpene,pigment,phytosterol,coumarin,organic acid and etc,plays significant roles in various physiological activities in organisms.The active ingredients generally interfere in signal transduction of cancer cells,regulate cell cycle and apoptotic protein expression,inhibit cancer cells proliferation and migration and etc,which effectively restrains tumor development and deterioration.This article summarizes the anti-tumor mechanism of five active ingredients in dandelion through paper reading which provides thoughts and references for anti-tumor research.展开更多
The architectural features of dandelion seeds contribute to their excellent flight ability.Previous studies have primarily focused on the interaction between air flow and seeds when wind passes through dandelion seeds...The architectural features of dandelion seeds contribute to their excellent flight ability.Previous studies have primarily focused on the interaction between air flow and seeds when wind passes through dandelion seeds from pappus bottom to pappus top(called‘type-I wind'hereafter).However,it is also common that wind passes through dandelion seeds from pappus top to pappus bottom(called‘type-II wind'hereafter)during abscission on the inflorescence head or flight in the air,due to the atmospheric turbulence and the diversity in architectural features.Nevertheless,the interaction between airflow and seeds in the type-II wind has not yet been investigated.In this study,we conducted numerical investigations on the variations in drag coefficients of dandelion pappus models and flow patterns around them in both wind types,considering changes in wind speed,pappus angle,and pappus porosity.Generally,flow patterns and drag coefficients could be significantly altered due to reversing wind direction.The disturbance of pappus'filaments to air flow is moved from the leeward side to the windward side when the wind changes from type-I to type-II,leading to that vortex rings in the type-II wind to be more likely to attach to the central disk of non-flattened dandelion pappus models while separation vortex rings occur in the type-I wind.The contribution of the pressure-induced drag force to the total drag force is largely promoted when the wind changes from type I to type II,resulting in the enhancement of the drag coefficients of non-flattened dandelion pappus models.However,the enhancement role may be weakened as wind speed,pappus angle,and pappus porosity increase.These findings will be helpful for further quantitative prediction of seed dispersal by wind and guiding the design of micro bionic air vehicles.展开更多
Intercropping of crops that can secrete bacteriostatic active substances can not only inhibit the occurrence of disease but also have an important effect on plant growth.However,the effects of dandelion intercropping ...Intercropping of crops that can secrete bacteriostatic active substances can not only inhibit the occurrence of disease but also have an important effect on plant growth.However,the effects of dandelion intercropping on pepper blight control and pepper growth remain unclear.In this study,the control effect of dandelion on pepper blight was studied by inoculating the pepper leaves with Phytophthora infestans,and it also discusses the correlation of the occurrence of pepper epidemic disease with the pepper canopy environment,soil environment,pepper photo-synthesis,and yield index.The results showed that best plant distance for dandelion intercropping was 20 cm(P20),and the control effect reached 43.31%.As compared to the CK,SOD enzyme,POD enzyme,and PAL enzyme were significantly up-regulated during the growth of pepper;chlorophyll content in pepper leaves was significantly increased;photosynthetic characteristics were significantly increased;stem diameter and yield of crop pepper were effectively improved;and the quality of the pepper product was better,but intercropping dandelion resulted in a significant decrease of nutrients in the soil environment of pepper,so a reasonable intercropping distance was needed.The correlation analysis shows that the incidence of pepper blight(A)was significantly positively correlated with soil temperature(Q),intercellular carbon dioxide(L),and canopy air temperature(O).The incidence of capsicum blight(A)was significantly negatively correlated with chlorophyll content(F),net photo-synthetic rate(K),stomatal conductance(M),ww rate(N),soil sucrase activity(W),vitamin C(AB),and leaf PAL enzyme(J).Finally,it was deduced that intercropping dandelion could effectively control the occurrence of pepper blight while also demonstrating a complex interaction with the pepper growing environment.展开更多
Background: A dandelion is a common plant with a global growth distribution that has been used as a medicinal and food with no adverse effects. Purpose: In this article, the products and effects of dandelions are revi...Background: A dandelion is a common plant with a global growth distribution that has been used as a medicinal and food with no adverse effects. Purpose: In this article, the products and effects of dandelions are reviewed to help further in-depth studies and develop more products derived from dandelions in the future. Method: The literature about dandelion in various databases such as Pubmed is searched. Results: Dandelions have many effects, such as virus inhibition, anti-tumor activity, nutritional value, anti-aging, potential as a vaccine and alleviation of heat stress. The mechanism underlying these effects is analyzed and it was found that dandelions were regulated by RNA or DNA. Conclusion: As a medicinal and food, dandelions are safe and have many effects. Many products derived from dandelions have been developed. The metabolic regulation is related with ribonucleic acid or possibly deoxynucleic acid. Further in-depth studies should be conducted on the regulation of dandelions through RNA or DNA. There will likely be more products derived from dandelions in the future.展开更多
基金supported by the Henan Provincial Science and Technology Research Project,China(242102111014)the Key Scientific Research Project of Colleges and Universities in Henan Province,China(23A230003)the Cultivation Program for Young Key Teachers and the Scientific Research Foundation for High-level Talents in Henan University of Technology,China(31401489)。
文摘Reducing aerobic spoilage and rumen greenhouse gas emissions from anaerobically fermented feeds remains critical challenges in energy saving and environmental protection of animal husbandry.This study investigated the effects of dandelions,both alone and combined with Lactobacillus plantarum and Lactobacillus buchneri(LAB)on fermentation quality,bacteriome and mycobiome after 180 d of anaerobic and 4 d of aerobic fermentation of whole-plant corn.In vitro dry matter digestion(IVDMD)and gas production from anaerobically fermented whole-plant corn were also assessed.The results demonstrated that dandelions,either alone or combined with LAB,effectively improved fermentation quality by reducing NH_(3)-N concentrations(22.72–25.99%)after anaerobic fermentation,decreasing the proliferation of yeast and molds to enhance the aerobic stability.Notably,the changes in the bacteriome were more pronounced than those in the mycobiome after aerobic exposure.The addition of dandelions or the combination reduced Acetobacter fabarum abundance,a member of the Acetobacter that was spoilage-induced microbe indicated by correlation analysis.Besides,these treatments facilitated competition relations of microbiome which contributed to the enhanced aerobic stability.Furthermore,dandelions reduced CH_(4)and CO_(2)emissions by 14.88 and 13.73%,respectively,and also positively influencing IVDMD by 4.46%.Collectively,dandelion alone or combined with LAB are promising strategies to improve the aerobic stability of anaerobically fermented whole-plant corn,a process linked to the interactions between the bacteriome and mycobiome,and to contribute to clean production by reducing rumen CH_(4)and CO_(2)emissions.
基金funded by the Liaoning Provincial Department of Education in 2023(No.JYTMS20231389)the Liaoning Provincial Science and Technology Department 2023 Joint Fund(No.2023-MSLH-297)+1 种基金the 2024 Shenyang Medical College Graduate Student Scientific and Technological Innovation Fund Project(No.Y20240533)the Liaoning Provincial Local Standards(DB/T 3476-2021),China。
文摘As a leafy vegetable,dandelion(Taraxacum antungense Kitag.)leaves are subject to senescence,such as yellowing and even decay,during postharvest storage.Calcium chloride(CaCl_(2))andε-polylysine(ε-PL)treatments can safely and effectively improve the postharvest quality of fruits and vegetables.To improve the storage quality,dandelions were treated with 0.5%(mass concentration)CaCl_(2)and 0.9 g/Lε-PL and stored at 4±0.5℃.The results showed that the combination treatment of CaCl_(2)andε-PL(CaCl_(2)+ε-PL treatment)could effectively delay the yellowing and chlorophyll content decline,as well as reduce the browning index,when compared with those of the control and single treatments.The CaCl_(2)+ε-PL treatment also enhanced the synthesis and accumulation of secondary metabolites.The combination treatment could significantly enhance the enzyme activities of phenylalanine ammonia-lyase(PAL),4-coumarate-Co A ligase(4CL),and cinnamate-4-hydroxylase(C4H)in phenylpropanoid metabolism and upregulate the expression levels of Ta PAL,Ta4CL,and Ta C4H.Meanwhile,ε-PL treatment was more effective than CaCl_(2)treatment in promoting the expression of these genes.Correlation analysis indicates that the transcription levels and activities of the three enzymes,as well as the contents of secondary metabolites,are linked to the quality changes of dandelions treated with CaCl_(2)+ε-PL during cold storage.Principal component analysis was used to assess the storage quality of dandelions during cold storage,and the results showed the following rankings:CaCl_(2)+ε-PL>ε-PL>CaCl_(2)>control.These findings suggest that CaCl_(2)+ε-PL treatment can improve the postharvest storage quality of dandelions by modulating the phenylpropane metabolic pathway.
基金supported by the Sir Joseph Pope Fellowship from Nottingham University,the High Performance Computing Platform of Xi'an Jiaotong University,and the National Natural Science Foundation of China(Grant Nos.12072254,12102323,and 12472108).
文摘In this paper,the effect of the morphological profile of dandelion seed on flight lift force under crosswind conditions is explored.Existing studies primarily focus on the flight characteristics of dandelion seed during its fall,emphasizing the influence of the complex filament structure on the formation of wake vortices.However,research on the flight lift force due to the dandelion seed’s morphological profile under lateral crosswind conditions is quite limited.This study investigates the aerodynamic behavior of dandelion seed using a novel virtual barrier model.This model is proposed,based on the regular pattern of the filaments’outer contours and the virtual barrier effect produced by their columnar array.Through elaborate numerical simulations,it is found that the morphological profile of dandelion seed possesses superior aerodynamic properties,particularly in generating lift force under crosswind conditions.This characteristic is a crucial mechanism for the long-distance dispersal of dandelion seed.Subsequently,the study extends to examine the aerodynamic performance of the model at varying degrees of opening angles and inflow attack angles,offering a fresh perspective on understanding the flight characteristics of dandelion seed in natural environments.The findings not only contribute to the field of plant aerodynamics but also provide insights into potential biomimetic applications in engineering.
基金supported by the National Natural Science Foundation of China(Grant No.52275373)the National Natural Science Foundation of China(Grant No.52105397)the Open Foundation of National Key Laboratory of Metal Forming Technology and Heavy Equipment(Grant No.S2308100.W08).
文摘The thermal deformation behavior of FV520B stainless steel is investigated.Isothermal compression tests were conducted at temperatures ranging from 600 to 900℃ and strain rates from 0.001 to 10 s^(−1).The true stress–strain curves were corrected for friction and temperature due to the drum shape and adiabatic heating.The comparison shows that there is a large difference between the stress before and after the correction,which proves that the correction is necessary.Five constitutive models were developed:the original Arrhenius model,the strain correction Arrhenius model,a new modified Arrhenius model,the back propagation neural network model(BPNN)and the dandelion optimization BPNN model(DO-BPNN).The DO-BPNN model showed the highest prediction accuracy though it was more computationally intensive than the other models.The new modified Arrhenius model performed a better predictive capacity than the strain correction version,while it showed a negligible increase in the number of parameters and computational time.Although artificial neural network-based models exhibit superior accuracy compared to the Arrhenius models,their application in finite element simulations still faces notable challenges.
基金supported by the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Grant No.SJCX24_0161)the National Natural Science Foundation of China(Grant Nos.61374153 and 61403199).
文摘To optimize the operating efficiency and extend the lifespan of the multistack fuel cell hybrid system(MFCHS),this paper proposes a two-layer multiobjective optimal energy management strategy that considers the degradation of the fuel cell and the battery.Regarding the issues that power fluctuations damage the fuel cells'lifespan and high-current charging and discharging lead to battery capacity decay,the first layer of the strategy adopts locally weighted scatterplot smoothing(LOWESS)to smooth the output power of the fuel cells and prevent the battery from operating under high-current conditions.The second layer considers the uneven degree of degradation among the fuel cells and employs the dandelion optimizer(DO)algorithm to solve the objective function with an aging adaptive factor,optimizing the efficiency and lifespan.Meanwhile,the DO algorithm is enhanced by tent chaotic mapping and differential variation to improve the convergence speed and accuracy.Compared with the equivalent hydrogen consumption minimization strategy(ECMS)and the equal distribution strategy,the proposed strategy improves the average operating efficiency of the fuel cells,effectively reduces the degradation of the fuel cells and the capacity degradation of the battery,and maintains the performance consistency among the fuel cells.
基金supported by the National Natural Science Foundation of China(Nos.81202935 and 81773893)the National Major Scientific and Technological Special Project of China for “Significant New Drugs Development”(No.2017ZX09301060-001)+1 种基金the Natural Science Foundation of Hubei Province,China(No.2015CFB302)Fundamental Research Funds for the Central Universities “South-Central University for Nationalities”(No.CZY20025)。
文摘We investigated the liver protective activity of dandelion polyphenols(DP)against acetaminophen(APAP;Paracetamol)-induced hepatotoxicity.Mice were acclimated for 1 week and randomly divided into the following groups(n=9 per group):Control,APAP,APAP+DP(100 mg·kg^–1),APAP+DP(200 mg·kg^–1),and APAP+DP(400 mg·kg^–1)groups.Mice were pretreated with DP(100,200,and 400 mg·kg^–1)by oral gavage for 7 d before being treated with 350 mg·kg^–1 APAP for 24 h to induced hepatotoxicity.Severe liver injury was observed,and hepatotoxicity was analyzed after 24 h by evaluation of biochemical markers,protein expressions levels,and liver histopathology.Pretreatment with DP was able to restore serum liver characteristics(aspartate transaminase,AST;alanine aminotransferase,ALT;alkaline phosphatase,AKP),improve redox imbalance(superoxide dismutase,SOD;glutathione,GSH;malondialdehyde,MDA),and decrease inflammatory factors(tumor necrosis factor-α,TNF-α;interleukin-1β,IL-1β).Pretreatment with DP also significantly inhibited the expression levels of nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2).Furthermore,DP pretreatment could inhibit the apoptosis of liver cells caused by APAP through up-regulation of Bcl-2 and down-regulation of Bax and caspase-9 protein.DP also down-regulated p-JNK protein expression levels to inhibit APAP-induced mitochondrial oxidative stress and up-regulated the expression of Nrf-2 and its target gene HO-1.The histopathological staining demonstrated that DP pretreatment could inhibit APAP-induced hepatocyte infiltration,congestion,and necrosis.Our results demonstrate that DP pretreatment could protect against APAP-induced hepatic injury by activating the Nrf-2/HO-1 pathway and inhibition of the intrinsic apoptosis pathway.
基金supported by the Academic Research fund of Hoseo University in 2011-0069
文摘Plants of the genus Taraxacum, commonly known as dandelions, are used to treat breast cancer in traditional folk medicine. However, their use has mainly been based on empirical findings without sufficient scientific evidence. Therefore, we hypothesized that dandelions would behave as a Selective estrogen receptor modulator （SERM） and be effective as hormone replacement therapy （HRT） in the postmenopausal women. In the present study, in vitro assay systems, including cell proliferation assay, reporter gene assay, and RT-PCR to evaluate the mRNA expression of estrogen-related genes （pS2 and progesterone receptor, PR）, were performed in human breast cancer cells. Dandelion ethanol extract （DEE） significantly increased cell proliferation and estrogen response element （ERE）-driven luciferase activity. DEE significantly induced the expression of estrogen related genes such as pS2 and PR, which was inhibited by tamoxifen at 1 gmol L-1. These results indicated that DEE could induce estrogenic activities mediated by a classical estrogen receptor pathway. In addition, immature rat uterotrophic assay was carried out to identify estrogenic activity of DEE in vivo. The lowest concentration of DEE slightly increased the uterine wet weight, but there was no significant effect with the highest concentration of DEE. The results demonstrate the potential estrogenic activities of DEE, providing scientific evidence supporting their use in traditional medicine.
文摘The protection of the liver as an essential organ in the body against oxidative stress and deleterious compounds has been the subject of recent investigations.Among different compounds,medicinal plants play an important role due to their hepatoprotective effects.Taraxacum officinale or"common dandelion"is a popular plant that has been traditionally used for its hepatoprotective effects.Currently,there are limited clinical studies on its hepatoprotective effects.The aim of this review article is to evaluate the hepatoprotective effects of dandelion and its mechanism of action.We reviewed literature up to July 2019 on"Taraxacum officinale"or"dandelion"and hepatoprotection.Currently available pharmacological studies indicate that dandelion extracts have hepatoprotective effects against chemical agents due to its antioxidant and antiinflammatory activities.The anti-inflammatory effects of dandelion,the prebiotic effects of its oligofructans,inhibitory effects against the release of lipopolysaccharides and fasting induced adipose factor,digestive enzymes,and enhancing effects of lipogenesis,reduce lipid accumulation and liver inflammation,which directly or indirectly improve the liver functions.Given emerging evidence on hepatoprotective effects of dandelion,designing large human clinical studies is essential.
基金Supported by the PhD Start-up Fund of Liaoning Province:Effect of Dandelion Flavonoids on Expression of Collagen Fiber Gene in Liver Cells of Mice(201501099)
文摘The paper was to study DPPH （1,1-diphenyl-2-trinitrobenzene hydrazine） scavenging ability of different concentrations of tlavonoid and polysaccheride from dandelion. Flavonoids were extracted from dandelion using alcohol precipitation ultrasonic extraction method and polysaccharide was extracted using water ul- trasonic extraction method. DPPH free radical scavenging ability of flavonoid and polysaccharide was determined using ultravialet spectrophotometer method. The difference in DPPH free radical scavenging ability between flavonoids and polysacchafide was analyzed statistically. The results showed that DPPH scavenging ability was the best when the concentration of flavanoid was 1.6 mg/mL; the scavenge rate was up to 96.11% and I Cso （median inhibitory concentration of inhibi^rs） was 0.38 mg/mL. DPPH scavenging ability was the best when the concentration of polysaccheride was 1.4 mg/mL; the scavenge rate was 89.33% and IC5o was 0.51 mg/mL. Statistical results showed that there were significant differences in DPPH free radical scavenging ability between dandelion flavonoid and polysaeoharide （P 〈 0.05 ）. Flavonoid had significantly higher scavenging ability than polysaccharide （P 〈 0.05）. The study lays a theoretical foundation for further research on dan- delion flavonoid, and provides a theoretical basis for development and utilization of dandelion resources.
基金supported by the Institutional Fund Projects(IFPIP-1481-611-1443)the Key Projects of Natural Science Research in Anhui Higher Education Institutions(2022AH051909)+1 种基金the Provincial Quality Project of Colleges and Universities in Anhui Province(2022sdxx020,2022xqhz044)Bengbu University 2021 High-Level Scientific Research and Cultivation Project(2021pyxm04)。
文摘A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all optimization problems. A self-adapting and efficient dandelion algorithm is proposed in this work to lower the number of DA's parameters and simplify DA's structure. Only the normal sowing operator is retained;while the other operators are discarded. An adaptive seeding radius strategy is designed for the core dandelion. The results show that the proposed algorithm achieves better performance on the standard test functions with less time consumption than its competitive peers. In addition, the proposed algorithm is applied to feature selection for credit card fraud detection(CCFD), and the results indicate that it can obtain higher classification and detection performance than the-state-of-the-art methods.
基金The authors greatly appreciate the financial support of the National Natural Science Foundation of China(11502155)Natural Science Foundation of Shanxi Province(201901D211112)the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2019L0328).We thank Prof.Yuren Wang and Mrs.Tingt-ing Zhao for discussions.
文摘It is of great significance to study the water absorption and expansion of cells,which are essential for the growth and life regulation of plants,not only for understanding the complex life phenomena of plants,but also for the manufacture of biomimetic materials.The main purpose of this study is to shed light on the mechanism of a novel coiling phenomenon of the split dandelion scape soaked in water and the high concentration mannitol solution.We discovered that the difference between water absorption/dehydration capacities of inner cells and lateral cells of the split dandelion scape generates asymmetric expansion/contraction between inner and lateral layers,resulting two opposite coiled patterns.Analogous to the deformable hypothesis of the thermal expansion model,the water absorption-induced cell expansion model was proposed to explain the coiling process of the split dandelion scape.Both the simulation and experimental results revealed that the coiling of dandelion scape is strongly depended on the response of cells to the stimulation of the solution environment.Showing considerable potential load bearing capacity of plants,the coiling phenomenon can be an inspiration to the designs of soft robots and bionic sensors for humidity control.
基金supported by the funding“Innovation Project of Shandong Province Agricultural Application Technology”(2130106)“Key Technology Research and Development Program of Shandong”(2019GNC106004).
文摘This novel study identifi es the effective anti-inflammatory phenolic compounds in dandelion and provides mechanistic insights into their interactions with receptor proteins(toll-like receptor 4,TLR4;co-receptor myeloid differentiation protein-2,MD-2)using UHPLC-ESI-MS/MS,lipopolysaccharide(LPS)-stimulated THP-1 cell line,fluorescence quenching and anisotropy,molecular docking(single ligand and multi-ligand docking)and molecular dynamics simulation.A 50%aqueous methanol extract had a greater anti-inflammatory effect and higher chicoric acid content,compared with the 100%water and 100%methanol extracts.Chicoric acid,chlorogenic acid,methylophiopogonone A,caffeic acid,gallic acid monohydrate and 4’-O-demethylbroussonin A had relatively high binding energies and contents in all extracts.Chicoric acid competed with chlorogenic acid,4’-O-demethylbroussonin A and quercetin for MD-2.Among dandelion’s phenolics,chicoric acid most effectively hindered TLR4-MD-2 complex formation,with a quenching constant of 0.62×10^(6) L/mol for MD-2 or TLR4 at 320 K,and binding energies of-6.87 and-5.97 kcal/mol,respectively,for MD-2 and TLR4.
基金This study was supported by Fund of Hubei Provincial Education Department(Q20204508)Fund of Jiangxi Provincial Education Department(GJJ190851)。
文摘As a traditional Chinese herb,dandelion,containing complicated active ingredients which includes polysaccharide,flavonoid,terpene,pigment,phytosterol,coumarin,organic acid and etc,plays significant roles in various physiological activities in organisms.The active ingredients generally interfere in signal transduction of cancer cells,regulate cell cycle and apoptotic protein expression,inhibit cancer cells proliferation and migration and etc,which effectively restrains tumor development and deterioration.This article summarizes the anti-tumor mechanism of five active ingredients in dandelion through paper reading which provides thoughts and references for anti-tumor research.
基金This work was supported by the High-Level Talent Training Program at Chengdu University(Grant No.2081921086).
文摘The architectural features of dandelion seeds contribute to their excellent flight ability.Previous studies have primarily focused on the interaction between air flow and seeds when wind passes through dandelion seeds from pappus bottom to pappus top(called‘type-I wind'hereafter).However,it is also common that wind passes through dandelion seeds from pappus top to pappus bottom(called‘type-II wind'hereafter)during abscission on the inflorescence head or flight in the air,due to the atmospheric turbulence and the diversity in architectural features.Nevertheless,the interaction between airflow and seeds in the type-II wind has not yet been investigated.In this study,we conducted numerical investigations on the variations in drag coefficients of dandelion pappus models and flow patterns around them in both wind types,considering changes in wind speed,pappus angle,and pappus porosity.Generally,flow patterns and drag coefficients could be significantly altered due to reversing wind direction.The disturbance of pappus'filaments to air flow is moved from the leeward side to the windward side when the wind changes from type-I to type-II,leading to that vortex rings in the type-II wind to be more likely to attach to the central disk of non-flattened dandelion pappus models while separation vortex rings occur in the type-I wind.The contribution of the pressure-induced drag force to the total drag force is largely promoted when the wind changes from type I to type II,resulting in the enhancement of the drag coefficients of non-flattened dandelion pappus models.However,the enhancement role may be weakened as wind speed,pappus angle,and pappus porosity increase.These findings will be helpful for further quantitative prediction of seed dispersal by wind and guiding the design of micro bionic air vehicles.
基金supported by the Young Talent Project of Northeast Agricultural University(20QC03)Disciplinary Team Project of Northeast Agricultural University.
文摘Intercropping of crops that can secrete bacteriostatic active substances can not only inhibit the occurrence of disease but also have an important effect on plant growth.However,the effects of dandelion intercropping on pepper blight control and pepper growth remain unclear.In this study,the control effect of dandelion on pepper blight was studied by inoculating the pepper leaves with Phytophthora infestans,and it also discusses the correlation of the occurrence of pepper epidemic disease with the pepper canopy environment,soil environment,pepper photo-synthesis,and yield index.The results showed that best plant distance for dandelion intercropping was 20 cm(P20),and the control effect reached 43.31%.As compared to the CK,SOD enzyme,POD enzyme,and PAL enzyme were significantly up-regulated during the growth of pepper;chlorophyll content in pepper leaves was significantly increased;photosynthetic characteristics were significantly increased;stem diameter and yield of crop pepper were effectively improved;and the quality of the pepper product was better,but intercropping dandelion resulted in a significant decrease of nutrients in the soil environment of pepper,so a reasonable intercropping distance was needed.The correlation analysis shows that the incidence of pepper blight(A)was significantly positively correlated with soil temperature(Q),intercellular carbon dioxide(L),and canopy air temperature(O).The incidence of capsicum blight(A)was significantly negatively correlated with chlorophyll content(F),net photo-synthetic rate(K),stomatal conductance(M),ww rate(N),soil sucrase activity(W),vitamin C(AB),and leaf PAL enzyme(J).Finally,it was deduced that intercropping dandelion could effectively control the occurrence of pepper blight while also demonstrating a complex interaction with the pepper growing environment.
文摘Background: A dandelion is a common plant with a global growth distribution that has been used as a medicinal and food with no adverse effects. Purpose: In this article, the products and effects of dandelions are reviewed to help further in-depth studies and develop more products derived from dandelions in the future. Method: The literature about dandelion in various databases such as Pubmed is searched. Results: Dandelions have many effects, such as virus inhibition, anti-tumor activity, nutritional value, anti-aging, potential as a vaccine and alleviation of heat stress. The mechanism underlying these effects is analyzed and it was found that dandelions were regulated by RNA or DNA. Conclusion: As a medicinal and food, dandelions are safe and have many effects. Many products derived from dandelions have been developed. The metabolic regulation is related with ribonucleic acid or possibly deoxynucleic acid. Further in-depth studies should be conducted on the regulation of dandelions through RNA or DNA. There will likely be more products derived from dandelions in the future.
