[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta ...[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced（COⅠ 1 539 bp,COⅡ 684 bp,CO Ⅲ 792 bp,with the total of 3 015 bp）.The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment.Phylogenic trees of four subspecies of L.migratoria were constructed by Neighbor-Joining,Maximum-parsimony and Bayesian,respectively.[Result] The average content of A ＋ T in three subunits of four subspecies was 69.57%;the third site of codon showed the highest A ＋ T content,and the COⅠ had the highest A ＋ T content（87.6%）;The nucleotide substitution mainly occurred at the third site of codon,and the nucleotide replacement rate of CO Ⅱ was the highest.The second site of codon was conservative,so the replacement rate was in the range of 5.9%-15%.The start codon of COⅠ was CCG or ACG.Genetic distances among four subspecies were ranged from 0.001 to 0.076.The relationship between L.m.tibetensis and Locusta migratoria manilensis was the closest,followed by L.m.migratorioides and L.m.migratorioides,while the genetic distance between L.m.tibetensis and L.m.migratorioides was the largest.[Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L.m.tibetensis,L.m.manilensis,L.m.migratoria,L.m.migratorioides.展开更多
The phylogenetic relationships of genera in the subfamily Apaturinae were examined using mtDNA sequence data from 1,471 bp of cytochrome oxidase subunitⅠ(COI).The mitochondrial COI gene from a total of 16 species in ...The phylogenetic relationships of genera in the subfamily Apaturinae were examined using mtDNA sequence data from 1,471 bp of cytochrome oxidase subunitⅠ(COI).The mitochondrial COI gene from a total of 16 species in 11 genera were sequenced to obtain mtDNA data,along with those of 4 species obtained from GenBank,to construct the MP and the NJ trees using Athyma jina,Penthema adelma,Polyura nepenthes,and Charaxes bernardus as outgroups.The transitions at the third codon positions of the COI data set were found saturated,but they were retained for analysis,because they contain the majority of the phylogenetic information.The impacts of equal weight assumptions for all characters in the parsimonious analysis were assessed by potential alternations in clades in response to different transition/transversion weighting schemes.The results indicated four distinct major groups in Apaturinae.Moreover,several well supported and stable clades were found in the Apaturinae.The study also identified undetermined taxon groups whose positions were weakly supported and were subject to changes under different weighting schemes.Within the Apaturinae,the clustering results are approximately identical to the classical morphological classification.The mtDNA data suggest the genus Mimathyma as a monophyletic group.Lelecella limenitoides and Dilipa fenestra have close relationship with very strong support in all phylogenetic trees.It also supports the taxonomic revision of removing several species from Apatura to other genera,namely Mimathyma schrenckii,M.chevana,M.nycteis,Chitoria subcaerulea,C.fasciola,C.pallas,and Helcyra subalba.展开更多
The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1 471 bp of mtDNA cytochrome oxidase subunit I （COI） gene sequence data which were obtained from 21 individuals spanning 9 genera, alon...The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1 471 bp of mtDNA cytochrome oxidase subunit I （COI） gene sequence data which were obtained from 21 individuals spanning 9 genera, along with those of 17 species obtained from GenBank, using Apatura iris, Aglais urticae, and Polyura dolon as outgroup species. Although the transitions at the third codon positions of the COI data set were highly saturated, they were still retained for analysis as they contain the majority of the phylogenetic information, and thus, the maximum pasimony （MP） under different weighting schemes and maximum likelihood （ML） trees were reconstructed in this study. The results showed that within this subfamily, the results based on the COI gene sequences are approximately identical to the traditional classification results. However, the clustering of Lexias pardalis and Tanaecia julii within the genus Euthalia as well as the clustering of Phaedyma aspasia within the genus Neptis with weak support are different from that of the current classification scheme made by Chinese scholars. The genus Limenitis is splited into two subclusters in the trees constructed by using MP and ML methods. These results support one of the strongest hypotheses for the tribe relationships within Limenitidinae.展开更多
Retinal ganglion cells in the rat were studied using the heavy metal intensified oytochrome oxidase and horseradish peroxidase histochemieal methods. The results show that a population of large retinal ganglion cells ...Retinal ganglion cells in the rat were studied using the heavy metal intensified oytochrome oxidase and horseradish peroxidase histochemieal methods. The results show that a population of large retinal ganglion cells was consistently observed with the eyto3hrome oxidase staining method in retinas of normal rats or rats which received unilateral thalamotomy at birrth. These oytochrome oxidase rich ganglion cells appeared to have large somata, 3-6 primary dendrites and extensive dendritic arbors, and are comparable to ganglion cells labeled by the wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP). However, the morphological details of some of the cells revealed by the cytoahrome oxidase staining method are frequently better than those shown by the HRP histochemieal method. These results suggest that the mit03hondrial enzyme oytoohrome oxidase can be used as a simple but reliable marker for identifying and studying a population of retinal ganglion cells with high metabolie rate in the rat.展开更多
We determined the sequence of mitochondrial Cytochrome oxidase subunit I （CO1） in two Drosophila melanogaster strains originating at ＂Evolution Canyon＂, Israel. CO1 nucleotide sequences from two iso-female strains...We determined the sequence of mitochondrial Cytochrome oxidase subunit I （CO1） in two Drosophila melanogaster strains originating at ＂Evolution Canyon＂, Israel. CO1 nucleotide sequences from two iso-female strains, 2-1 and 6-1, were 1,534 and 1,543 base-pairs, respectively. In each strain, ATAA was used in initiation of translation. Exchange rates for nucleotide and amino acid sequences were larger in the 6-1 strain than in the 2-1 strain when Oregon-R was used as the standard. Non-synonymous exchange rate was larger than synonymous exchange rate among the three strains.展开更多
Unambiguous and rapid sarcosaphagous insect species identification is an essential requirement for forensic investigations. Although some insect species are difficult to classify morphologically, they can be effective...Unambiguous and rapid sarcosaphagous insect species identification is an essential requirement for forensic investigations. Although some insect species are difficult to classify morphologically, they can be effectively identified using molecular methods based on similarity with abundant authenticated reference DNA sequences in local databases. However, local databases are still relatively incomplete in China because of the large land area with distinct regional conditions. In this study, 75 forensically important blow flies were collected from 23 locations in 16 Chinese provinces, and a 278-bp segment of the cytochrome oxidase subunit I gene of all specimens was successfully sequenced. Phylogenetic analysis of the sequenced segments showed that all Calliphorid specimens were properly assigned into nine species with relatively strong supporting values, thus indicating that the 278-bp cytochrome oxidase subunit one region is suitable for identification of Calliphorid species. The clear difference between intraspecific threshold and interspecific divergence confirmed the potential of this region for Calliphorid species identification, especially for distinguishing between morphologically similar species. Intraspecific geographic variations were observed in Lucilia sericata （Meigen, 1826） and Lucilia caesar （Linnaeus, 1758）.展开更多
Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with...Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with four species from the GenBank nucleotide database.The purpose of this study was analyzing the phylogenetic relationships among subfamilies within Pamphagidae and interpreting the phylogenetic position of this family within the Acridoidea superfamily.Phylogenetic trees were reconstructed using neighbor-joining(NJ),maximurn parsimony(MP)and Bayesian inference(BI)methods.The 684 bp analyzed fragment included 126 parsimony informative sites.Sequences diverged 1.0%-11.1%between genera within subfamilies,and 8.8%-12.3%between subfamilies.Amino acid sequence diverged 0-6.1%between genera within subfamilies,and 0.4%-7.5%between subfamilies.Our phylogenetic trees revealed the monophyly of Pamphagidae and three distinct major groups within this family.Moreover,several well supported and stable clades were found in Pamphagidae.The global clustering results were similar to that obtained through classical morphological classification:Prionotropisinae,Thrinchinae and Pamphaginae were monophyletic groups.However,the current genus Filchnerella(Prionotropisinae)was not a monophyletic group and the genus Asiotmethis(Prionotropisinae)was a sister group of the genus Thrinchus(Thrinchinae).Further molec-ular and morphological studies are required to clarify the phylogenetic relationships of the genera Filchnerella and Asiotmethis.展开更多
Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide associ...Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study approach.One major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and GP.The candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/qGP6.The disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle process.The glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed germination.The elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed germination.Thus,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.展开更多
The present study established a rat model of chronic cerebral ischemia using bilateral common carotid artery permanent ligation to analyze cytochrome C oxidase activity and mRNA expression in hippocampal mitochondria....The present study established a rat model of chronic cerebral ischemia using bilateral common carotid artery permanent ligation to analyze cytochrome C oxidase activity and mRNA expression in hippocampal mitochondria. Results showed significantly decreased cytochrome C oxidase activity and cytochrome C oxidase II mRNA expression with prolonged ischemia time. Further analysis revealed five mitochondrial cytochrome C oxidase II gene mutations, two newly generated mutations, and four absent mutational sites at 1 month after cerebral ischemia, as well as three mitochondrial cytochrome C oxidase III gene mutations, including two newly generating mutations, and one disappeared mutational site at 1 month after cerebral ischemia. Results demonstrated that decreased cytochrome C oxidase gene expression and mutations, as well as decreased cytochrome C oxidase activity, resulting in energy dysmetabolism, which has been shown to be involved in the DatholoQical Process of ischemic brain iniurv.展开更多
ObJective： To investigate the detrimental effects of hemorrhagic shock on the structure and function of mitochondria DNA （mtDNA） encoding cytochrome oxidase genes in intestinal epithelial cells. Methods ： Wistar ...ObJective： To investigate the detrimental effects of hemorrhagic shock on the structure and function of mitochondria DNA （mtDNA） encoding cytochrome oxidase genes in intestinal epithelial cells. Methods ： Wistar rats were used and divided into two groups： hemorrhagic shock group and control group. Hemorrhagic shock model of rats was utilized in this experiment. The mtDNA was extracted from the intestinal epithelial cells and amplified by polymerase chain reaction （ PCR ） with different primers of cytochrome oxidase （ COX Ⅰ, COX Ⅱ and COX Ⅲ）. The products of PCR were directly sequenced. Results ： Hemorrhagic shock could result in the point mutagenesis in mitochondrial genome encoding cytochrome oxidase （ COX I and COX II）. There were 4, 4, 22, 16, 35 point mutations in COX I from 5545 to 6838 bp in 5 shocked rats. There were five point mutations in COX II from 7191 to 7542 bp at the site of t7191c, t7212c, a7386g, a7483g, c7542g in 1 shocked rat. There was no mutation found in COX III. Conclusions： Hemorrhagic shock could significantly induce the damage of the gene of cytochrome oxidase encoded by mtDNA.展开更多
The generation of cellular energy in the form of ATP occurs mainly in mitochondria by oxidative phosphorylation.Cytochrome c oxidase(CytOx),the oxygen accepting and rate-limiting step of the respiratory chain,regulate...The generation of cellular energy in the form of ATP occurs mainly in mitochondria by oxidative phosphorylation.Cytochrome c oxidase(CytOx),the oxygen accepting and rate-limiting step of the respiratory chain,regulates the supply of variable ATP demands in cells by“allosteric ATP-inhibition of CytOx.”This mechanism is based on inhibition of oxygen uptake of CytOx at high ATP/ADP ratios and low ferrocytochrome c concentrations in the mitochondrial matrix via cooperative interaction of the two substrate binding sites in dimeric CytOx.The mechanism keeps mitochondrial membrane potentialΔΨm and reactive oxygen species(ROS)formation at low healthy values.Stress signals increase cytosolic calcium leading to Ca^2+-dependent dephosphorylation of CytOx subunit I at the cytosolic side accompanied by switching off the allosteric ATPinhibition and monomerization of CytOx.This is followed by increase ofΔΨm and formation of ROS.A hypothesis is presented suggesting a dynamic change of binding of NDUFA4,originally identified as a subunit of complex I,between monomeric CytOx(active state with highΔΨm,high ROS and low efficiency)and complex I(resting state with lowΔΨm,low ROS and high efficiency).展开更多
The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation unde...The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation under well-defined experimental conditions. The experiments were conducted in a specialized microwave processing apparatus, with an exposure frequency of 18 GHz, and a temperature profile that was restricted to below 40℃ to avoid thermal degradation of the bacteria. The absorbed power was calculated to be 1,500 kW/m3 and the electric field was determined to be 300 Wm. Both values were theoretically confirmed using Computer Simulation Technology （CST） Microwave Studio 3D Electromagnetic Stimulation Software. Results showed that the activity of both enzymes was increased following MW radiation compared to negative controls and thermally treated samples subjected to similar temperature profiles. It is suggested that the increase in COX and LDH enzyme activity could not be explained by conventional heating alone, but was rather a result of micro-thermal effects that incorporated ＇undetectable＇ thermal mechanisms.展开更多
Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell l...Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was展开更多
Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integ...Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.展开更多
The Saccostrea mordax Gould,1850 is a typical intertidal species,whose genetic differentiation is influenced by various factors,including geological and climatic changes.To explore the genetic structure and historical...The Saccostrea mordax Gould,1850 is a typical intertidal species,whose genetic differentiation is influenced by various factors,including geological and climatic changes.To explore the genetic structure and historical population characteristics of Saccostrea mordax,we sequenced the mitochondrial cytochrome c oxidase subunit I(COI)gene from 58 specimens sampled from four locations in the western Pacific.Additionally,103 individuals from the Persian Gulf and western Pacific(from databases)were included for phylogenetic analysis.The Bayesian Inference tree showed that all specimens were divided into two clades,i.e.,the Persian Gulf population and the western Pacific population.Spatial molecular variance analysis indicated significant genetic differentiation between the two populations,and isolation by distance analysis revealed a positive correlation between genetic differentiation and geographic distance.Neutrality tests and Bayesian Skyline Plot suggested that both populations underwent expansions during the late Pleistocene.This study revealed the population history of Saccostrea mordax and described a new lineage,Saccostrea mordax lineage D,providing a foundation for understanding oyster biodiversity formation and genetic resource conservation.展开更多
To explore the roles of mitochondria tRNA leu(UUR) gene mutation at nucleotide 3243 and the activity of cytochrome c oxidase in pathogenesis of preeclampsia, 57 patients with preeclampsia and 60 normotension ...To explore the roles of mitochondria tRNA leu(UUR) gene mutation at nucleotide 3243 and the activity of cytochrome c oxidase in pathogenesis of preeclampsia, 57 patients with preeclampsia and 60 normotension pregnancy women were screened for tRNA leu(UUR) nt3243 A→G mutation with the method of polymers chain reaction (PCR) and restriction fragment length polymorphism. Cytochrome c oxidase activity was determined by measuring the rate of cyanide sensitive oxidation of reduced cytochrome c using luminosity photographer. The results showed that cytochrome c oxidase activity was significantly lower in the preeclampsia group (0.30±0.39/min, n = 32) than that in the controls (0.73±0.54/min, n = 26, P <0.01). The mitochondria DNA mutation at position 3243 was not found in our series. The results suggested that the decreased activity of cytochrome c oxidase might impair the energy production, leading to the mitochondria dysfunction and placenta dysfunction in preeclampsia patients. Mitochondria dysfunction may be involved in the pathogenesis of preeclampsia. The mutation of mitochondria DNA may not be the common contributor of preeclampsia in our series.展开更多
In order to clarify the mitochondrial bar code sequence and taxonomic information of common goby fish in Tianjin coastal marine areas,seven species of goby fish were collected from Tianjin coastal marine areas,identif...In order to clarify the mitochondrial bar code sequence and taxonomic information of common goby fish in Tianjin coastal marine areas,seven species of goby fish were collected from Tianjin coastal marine areas,identified by morphology,and DNA was extracted for amplification and sequencing of mitochondrial COI gene fragments.Phylogenetic analysis and molecular identification software MEGA were used to analyze the sequence characteristics and molecular classification of COI gene fragments of 12 common goby species in Tianjin coastal marine areas.Seven COI gene fragments of Goby were amplified and sequenced,the average GC content was lower than AT content,the base had a bias in the codon position distribution,and the highest GC content was the second base,which was similar to the COI gene base of many fishes.The codon base variation rate was 19.4%,and the ratio of transitions to transversions was 1.3.Phylogenetic distance analysis and phylogenetic tree analysis clarified the phylotaxonomic and evolutionary relationships of 12 major goby species in Tianjin coastal marine areas.Genetic distance indicates that Amblychaeturichthys hexanema and Synechogobius ommaturus are the same species.The high codon variation rate of codon encoding gene in Goby may be related to its good environmental adaptability.展开更多
Background:The thermogenic characteristics of animals are closely related to species distribution,and basal metabolic rate(BMR)and thermal neutral zone(TNZ)are important components of the thermogenic process in animal...Background:The thermogenic characteristics of animals are closely related to species distribution,and basal metabolic rate(BMR)and thermal neutral zone(TNZ)are important components of the thermogenic process in animals.Furthermore,the cytochrome c oxidase 1(COX1)gene has become a subject of particular interest due to its high degree of sequence conservation,stable evolutionary rate,and rare insertions/deletions.Method:The present study selected 29 species of Rodentia and 20 species of Chiroptera.The present study employed the statistical software SPSS(27.0 Chinese version)and Origin(2024 Chinese version)software to conduct correlation analyses on a variety of biological and ecological variables.These variables included body weight,BMR,TNZ,upper thermal neutral zone,lower thermal neutral zone(LTNZ),litter size,and dietary patterns of species from different latitudes.Furthermore,we conducted a series of phylogenetic tree analyses on COX1 protein.Results:As the geographic location of a species increases in latitude,there is an observed upward trend in both body mass and BMR of rodent species.Rodentia and Chiroptera species have been observed to exhibit a decrease in LTNZ and an expansion of TNZ.With regard to dietary habits,Rodentia species are predominantly phytophagous or omnivorous.Omnivorous species exhibit a marked tendency to produce larger litters in comparison to their herbivorous counterparts.Chiroptera species exhibit a diverse dietary range,including phytophagous,carnivorous,and omnivorous species.However,no correlation was observed between dietary differences and litter size.Phylogenetic analysis of the COX1 protein subsequently demonstrated that these two species groups share a monophyletic origin.Conclusion:The present study suggests that the selected Rodentia and Chiroptera species adapt to high-latitude environments by lowering the LTNZ and widening the TNZ.Furthermore,an upward trend has been observed in the body mass and BMR of high-latitude Rodentia.Phylogenetic analysis of the COX1 protein across various taxonomic groups substantiates the efficacy of this gene for species identification.Integrating physiological phenotypes with COX1 protein molecular evidence,this study provides a reference framework for the multidimensional mechanisms of mammalian latitudinal adaptation.展开更多
Understanding the genetic diversity–area relationship(GAR)is essential for comprehending how species adapt to environmental changes,as genetic diversity is an indicator of a species’adaptive potential.Variation in e...Understanding the genetic diversity–area relationship(GAR)is essential for comprehending how species adapt to environmental changes,as genetic diversity is an indicator of a species’adaptive potential.Variation in environmental adaptation capacity exists among species and animal taxa with different distribution areas,highlighting the importance of understanding the GAR.To obtain a more comprehensive understanding of the GAR in terrestrial vertebrates,we assessed both haplotype diversity–area and nucleotide diversity–area relationships using 25,453 cytochrome c oxidase subunit I(COI)sequences from 142 amphibian species,574 bird species,and 342 mammal species.We found that both measures of genetic diversity increased with species range size across major animal groups.Nevertheless,the GAR did not differ among animal groups,while haplotype diversity performed better than nucleotide diversity in profiling the GAR,as indicated by higher R2 values.The difference in the modeling fit may stem from the distinct biological and mathematical significance of nucleotide diversity and haplotype diversity.These results suggest that the GAR follows similar rules among different animal taxa.Furthermore,haplotype diversity may serve as a more reliable indicator for assessing the potential effects of area size changes on animal populations and provide better guidance for conserving genetic diversity.展开更多
Members of the passerine family Sylviidae are distributed widely around the Old World and the phylogenetic relationships of many species still remain controversial.In this study,we investigated the phylogeny and relat...Members of the passerine family Sylviidae are distributed widely around the Old World and the phylogenetic relationships of many species still remain controversial.In this study,we investigated the phylogeny and relationships among 37 species of 11 genera by analyzing DNA sequences obtained from the complete mitochondrial cytochrome b (cyt b) and partial cytochrome oxidase I (COI) genes.The data were analyzed by maximum-likelihood analysis and Bayesian inference.The results demonstrate that the current genus Phylloscopus is non-monophyletic,while Seicercus is synonymized with Phylloscopus or is a group within the genus Phylloscopus.We may conclude that within Phylloscopus there are close relations between P.magnirostris and P.borealis;among P.proregulus,P.yunnanensis,P.pulcher and P.maculipennis,as well as among P.occisinensis,P.schwarzi,P.armandii,P.collybita and P.fuscatus.Monophyly of the genus Cettia could not be corroborated;it is closely related to the genera Abroscopus and Tesia.However monophyly of Acrocephalus is supported.Furthermore,the close relationships between Sylvia and Zosterops and among Prinia,Orthotomus and Cisticola are also supported.展开更多
基金Supported by National Natural Science Foundation of China(30770263)~~
文摘[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced（COⅠ 1 539 bp,COⅡ 684 bp,CO Ⅲ 792 bp,with the total of 3 015 bp）.The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment.Phylogenic trees of four subspecies of L.migratoria were constructed by Neighbor-Joining,Maximum-parsimony and Bayesian,respectively.[Result] The average content of A ＋ T in three subunits of four subspecies was 69.57%;the third site of codon showed the highest A ＋ T content,and the COⅠ had the highest A ＋ T content（87.6%）;The nucleotide substitution mainly occurred at the third site of codon,and the nucleotide replacement rate of CO Ⅱ was the highest.The second site of codon was conservative,so the replacement rate was in the range of 5.9%-15%.The start codon of COⅠ was CCG or ACG.Genetic distances among four subspecies were ranged from 0.001 to 0.076.The relationship between L.m.tibetensis and Locusta migratoria manilensis was the closest,followed by L.m.migratorioides and L.m.migratorioides,while the genetic distance between L.m.tibetensis and L.m.migratorioides was the largest.[Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L.m.tibetensis,L.m.manilensis,L.m.migratoria,L.m.migratorioides.
基金This work was supported by National Natural Science Foundation of China(No.30570247)the Natural Science Foundation of Shanxi Province(No.2003-1087)
文摘The phylogenetic relationships of genera in the subfamily Apaturinae were examined using mtDNA sequence data from 1,471 bp of cytochrome oxidase subunitⅠ(COI).The mitochondrial COI gene from a total of 16 species in 11 genera were sequenced to obtain mtDNA data,along with those of 4 species obtained from GenBank,to construct the MP and the NJ trees using Athyma jina,Penthema adelma,Polyura nepenthes,and Charaxes bernardus as outgroups.The transitions at the third codon positions of the COI data set were found saturated,but they were retained for analysis,because they contain the majority of the phylogenetic information.The impacts of equal weight assumptions for all characters in the parsimonious analysis were assessed by potential alternations in clades in response to different transition/transversion weighting schemes.The results indicated four distinct major groups in Apaturinae.Moreover,several well supported and stable clades were found in the Apaturinae.The study also identified undetermined taxon groups whose positions were weakly supported and were subject to changes under different weighting schemes.Within the Apaturinae,the clustering results are approximately identical to the classical morphological classification.The mtDNA data suggest the genus Mimathyma as a monophyletic group.Lelecella limenitoides and Dilipa fenestra have close relationship with very strong support in all phylogenetic trees.It also supports the taxonomic revision of removing several species from Apatura to other genera,namely Mimathyma schrenckii,M.chevana,M.nycteis,Chitoria subcaerulea,C.fasciola,C.pallas,and Helcyra subalba.
基金supported by the National Natural Science Foundation of China (31071980, 40871034)the Youth Science Foundation of Shanxi Province, China(2010021029-3)the Postdoctoral Science Foun-dation of China (20090450652)
文摘The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1 471 bp of mtDNA cytochrome oxidase subunit I （COI） gene sequence data which were obtained from 21 individuals spanning 9 genera, along with those of 17 species obtained from GenBank, using Apatura iris, Aglais urticae, and Polyura dolon as outgroup species. Although the transitions at the third codon positions of the COI data set were highly saturated, they were still retained for analysis as they contain the majority of the phylogenetic information, and thus, the maximum pasimony （MP） under different weighting schemes and maximum likelihood （ML） trees were reconstructed in this study. The results showed that within this subfamily, the results based on the COI gene sequences are approximately identical to the traditional classification results. However, the clustering of Lexias pardalis and Tanaecia julii within the genus Euthalia as well as the clustering of Phaedyma aspasia within the genus Neptis with weak support are different from that of the current classification scheme made by Chinese scholars. The genus Limenitis is splited into two subclusters in the trees constructed by using MP and ML methods. These results support one of the strongest hypotheses for the tribe relationships within Limenitidinae.
文摘Retinal ganglion cells in the rat were studied using the heavy metal intensified oytochrome oxidase and horseradish peroxidase histochemieal methods. The results show that a population of large retinal ganglion cells was consistently observed with the eyto3hrome oxidase staining method in retinas of normal rats or rats which received unilateral thalamotomy at birrth. These oytochrome oxidase rich ganglion cells appeared to have large somata, 3-6 primary dendrites and extensive dendritic arbors, and are comparable to ganglion cells labeled by the wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP). However, the morphological details of some of the cells revealed by the cytoahrome oxidase staining method are frequently better than those shown by the HRP histochemieal method. These results suggest that the mit03hondrial enzyme oytoohrome oxidase can be used as a simple but reliable marker for identifying and studying a population of retinal ganglion cells with high metabolie rate in the rat.
文摘We determined the sequence of mitochondrial Cytochrome oxidase subunit I （CO1） in two Drosophila melanogaster strains originating at ＂Evolution Canyon＂, Israel. CO1 nucleotide sequences from two iso-female strains, 2-1 and 6-1, were 1,534 and 1,543 base-pairs, respectively. In each strain, ATAA was used in initiation of translation. Exchange rates for nucleotide and amino acid sequences were larger in the 6-1 strain than in the 2-1 strain when Oregon-R was used as the standard. Non-synonymous exchange rate was larger than synonymous exchange rate among the three strains.
文摘Unambiguous and rapid sarcosaphagous insect species identification is an essential requirement for forensic investigations. Although some insect species are difficult to classify morphologically, they can be effectively identified using molecular methods based on similarity with abundant authenticated reference DNA sequences in local databases. However, local databases are still relatively incomplete in China because of the large land area with distinct regional conditions. In this study, 75 forensically important blow flies were collected from 23 locations in 16 Chinese provinces, and a 278-bp segment of the cytochrome oxidase subunit I gene of all specimens was successfully sequenced. Phylogenetic analysis of the sequenced segments showed that all Calliphorid specimens were properly assigned into nine species with relatively strong supporting values, thus indicating that the 278-bp cytochrome oxidase subunit one region is suitable for identification of Calliphorid species. The clear difference between intraspecific threshold and interspecific divergence confirmed the potential of this region for Calliphorid species identification, especially for distinguishing between morphologically similar species. Intraspecific geographic variations were observed in Lucilia sericata （Meigen, 1826） and Lucilia caesar （Linnaeus, 1758）.
文摘Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit II(COIl)mtDNA sequences(684 bp).Twenty-seven species of Acridoidea from 20 genera were sequenced to obtain mtDNA data,along with four species from the GenBank nucleotide database.The purpose of this study was analyzing the phylogenetic relationships among subfamilies within Pamphagidae and interpreting the phylogenetic position of this family within the Acridoidea superfamily.Phylogenetic trees were reconstructed using neighbor-joining(NJ),maximurn parsimony(MP)and Bayesian inference(BI)methods.The 684 bp analyzed fragment included 126 parsimony informative sites.Sequences diverged 1.0%-11.1%between genera within subfamilies,and 8.8%-12.3%between subfamilies.Amino acid sequence diverged 0-6.1%between genera within subfamilies,and 0.4%-7.5%between subfamilies.Our phylogenetic trees revealed the monophyly of Pamphagidae and three distinct major groups within this family.Moreover,several well supported and stable clades were found in Pamphagidae.The global clustering results were similar to that obtained through classical morphological classification:Prionotropisinae,Thrinchinae and Pamphaginae were monophyletic groups.However,the current genus Filchnerella(Prionotropisinae)was not a monophyletic group and the genus Asiotmethis(Prionotropisinae)was a sister group of the genus Thrinchus(Thrinchinae).Further molec-ular and morphological studies are required to clarify the phylogenetic relationships of the genera Filchnerella and Asiotmethis.
基金supported by the Hainan Province Science and Technology Special Fund,China(ZDYF2023XDNY086)the Project of Sanya Yazhou Bay Science and Technology City,China(SCKJ-JYRC-2022-87)+2 种基金the Natural Science Foundation of Guangdong Province,China(2023A1515012052 and 2023A1515012092)the Guangzhou Science and Technology Plan Project,China(2023A04J1452 and 2023A04J0749)the Double First-class Discipline Promotion Project,China(2021B10564001).
文摘Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study approach.One major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and GP.The candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/qGP6.The disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle process.The glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed germination.The elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed germination.Thus,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.
基金the International Cooperation Program of Science and Technology Department of Jilin Province, No. 20100751
文摘The present study established a rat model of chronic cerebral ischemia using bilateral common carotid artery permanent ligation to analyze cytochrome C oxidase activity and mRNA expression in hippocampal mitochondria. Results showed significantly decreased cytochrome C oxidase activity and cytochrome C oxidase II mRNA expression with prolonged ischemia time. Further analysis revealed five mitochondrial cytochrome C oxidase II gene mutations, two newly generated mutations, and four absent mutational sites at 1 month after cerebral ischemia, as well as three mitochondrial cytochrome C oxidase III gene mutations, including two newly generating mutations, and one disappeared mutational site at 1 month after cerebral ischemia. Results demonstrated that decreased cytochrome C oxidase gene expression and mutations, as well as decreased cytochrome C oxidase activity, resulting in energy dysmetabolism, which has been shown to be involved in the DatholoQical Process of ischemic brain iniurv.
文摘ObJective： To investigate the detrimental effects of hemorrhagic shock on the structure and function of mitochondria DNA （mtDNA） encoding cytochrome oxidase genes in intestinal epithelial cells. Methods ： Wistar rats were used and divided into two groups： hemorrhagic shock group and control group. Hemorrhagic shock model of rats was utilized in this experiment. The mtDNA was extracted from the intestinal epithelial cells and amplified by polymerase chain reaction （ PCR ） with different primers of cytochrome oxidase （ COX Ⅰ, COX Ⅱ and COX Ⅲ）. The products of PCR were directly sequenced. Results ： Hemorrhagic shock could result in the point mutagenesis in mitochondrial genome encoding cytochrome oxidase （ COX I and COX II）. There were 4, 4, 22, 16, 35 point mutations in COX I from 5545 to 6838 bp in 5 shocked rats. There were five point mutations in COX II from 7191 to 7542 bp at the site of t7191c, t7212c, a7386g, a7483g, c7542g in 1 shocked rat. There was no mutation found in COX III. Conclusions： Hemorrhagic shock could significantly induce the damage of the gene of cytochrome oxidase encoded by mtDNA.
文摘The generation of cellular energy in the form of ATP occurs mainly in mitochondria by oxidative phosphorylation.Cytochrome c oxidase(CytOx),the oxygen accepting and rate-limiting step of the respiratory chain,regulates the supply of variable ATP demands in cells by“allosteric ATP-inhibition of CytOx.”This mechanism is based on inhibition of oxygen uptake of CytOx at high ATP/ADP ratios and low ferrocytochrome c concentrations in the mitochondrial matrix via cooperative interaction of the two substrate binding sites in dimeric CytOx.The mechanism keeps mitochondrial membrane potentialΔΨm and reactive oxygen species(ROS)formation at low healthy values.Stress signals increase cytosolic calcium leading to Ca^2+-dependent dephosphorylation of CytOx subunit I at the cytosolic side accompanied by switching off the allosteric ATPinhibition and monomerization of CytOx.This is followed by increase ofΔΨm and formation of ROS.A hypothesis is presented suggesting a dynamic change of binding of NDUFA4,originally identified as a subunit of complex I,between monomeric CytOx(active state with highΔΨm,high ROS and low efficiency)and complex I(resting state with lowΔΨm,low ROS and high efficiency).
文摘The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation under well-defined experimental conditions. The experiments were conducted in a specialized microwave processing apparatus, with an exposure frequency of 18 GHz, and a temperature profile that was restricted to below 40℃ to avoid thermal degradation of the bacteria. The absorbed power was calculated to be 1,500 kW/m3 and the electric field was determined to be 300 Wm. Both values were theoretically confirmed using Computer Simulation Technology （CST） Microwave Studio 3D Electromagnetic Stimulation Software. Results showed that the activity of both enzymes was increased following MW radiation compared to negative controls and thermally treated samples subjected to similar temperature profiles. It is suggested that the increase in COX and LDH enzyme activity could not be explained by conventional heating alone, but was rather a result of micro-thermal effects that incorporated ＇undetectable＇ thermal mechanisms.
文摘Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was
基金supported by grants from the Guangxi Natural Science Foundation(2024GXNSFAA010150)the Guangdong Basic and Applied Basic Research Foundation(2022A1515111167).
文摘Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.
基金Supported by the Key R&D Program of Shandong Province(No.2023CXGC010411)the National Key R&D Program of China(Nos.2023YFD2400800,2022YFD2401301,2022FY100304)+2 种基金the National Natural Science Foundation of China(Nos.42076092,41906083,41776179)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42000000)the Earmarked Fund for Modern Agro-industry Technology Research System(No.CARS-47)。
文摘The Saccostrea mordax Gould,1850 is a typical intertidal species,whose genetic differentiation is influenced by various factors,including geological and climatic changes.To explore the genetic structure and historical population characteristics of Saccostrea mordax,we sequenced the mitochondrial cytochrome c oxidase subunit I(COI)gene from 58 specimens sampled from four locations in the western Pacific.Additionally,103 individuals from the Persian Gulf and western Pacific(from databases)were included for phylogenetic analysis.The Bayesian Inference tree showed that all specimens were divided into two clades,i.e.,the Persian Gulf population and the western Pacific population.Spatial molecular variance analysis indicated significant genetic differentiation between the two populations,and isolation by distance analysis revealed a positive correlation between genetic differentiation and geographic distance.Neutrality tests and Bayesian Skyline Plot suggested that both populations underwent expansions during the late Pleistocene.This study revealed the population history of Saccostrea mordax and described a new lineage,Saccostrea mordax lineage D,providing a foundation for understanding oyster biodiversity formation and genetic resource conservation.
文摘To explore the roles of mitochondria tRNA leu(UUR) gene mutation at nucleotide 3243 and the activity of cytochrome c oxidase in pathogenesis of preeclampsia, 57 patients with preeclampsia and 60 normotension pregnancy women were screened for tRNA leu(UUR) nt3243 A→G mutation with the method of polymers chain reaction (PCR) and restriction fragment length polymorphism. Cytochrome c oxidase activity was determined by measuring the rate of cyanide sensitive oxidation of reduced cytochrome c using luminosity photographer. The results showed that cytochrome c oxidase activity was significantly lower in the preeclampsia group (0.30±0.39/min, n = 32) than that in the controls (0.73±0.54/min, n = 26, P <0.01). The mitochondria DNA mutation at position 3243 was not found in our series. The results suggested that the decreased activity of cytochrome c oxidase might impair the energy production, leading to the mitochondria dysfunction and placenta dysfunction in preeclampsia patients. Mitochondria dysfunction may be involved in the pathogenesis of preeclampsia. The mutation of mitochondria DNA may not be the common contributor of preeclampsia in our series.
基金supported by the National Key R&D Plan(2019YFD0902102 and 2019YFE01223000)Innovation Team Project of Marine Aquaculture Modern Agricultural Industry System in Tianjin City(ITTMRS2021005).
文摘In order to clarify the mitochondrial bar code sequence and taxonomic information of common goby fish in Tianjin coastal marine areas,seven species of goby fish were collected from Tianjin coastal marine areas,identified by morphology,and DNA was extracted for amplification and sequencing of mitochondrial COI gene fragments.Phylogenetic analysis and molecular identification software MEGA were used to analyze the sequence characteristics and molecular classification of COI gene fragments of 12 common goby species in Tianjin coastal marine areas.Seven COI gene fragments of Goby were amplified and sequenced,the average GC content was lower than AT content,the base had a bias in the codon position distribution,and the highest GC content was the second base,which was similar to the COI gene base of many fishes.The codon base variation rate was 19.4%,and the ratio of transitions to transversions was 1.3.Phylogenetic distance analysis and phylogenetic tree analysis clarified the phylotaxonomic and evolutionary relationships of 12 major goby species in Tianjin coastal marine areas.Genetic distance indicates that Amblychaeturichthys hexanema and Synechogobius ommaturus are the same species.The high codon variation rate of codon encoding gene in Goby may be related to its good environmental adaptability.
基金supported by the National Natural Scientific Foundation of China(32160254)Yunnan Fundamental Research Projects(202401AS070039).
文摘Background:The thermogenic characteristics of animals are closely related to species distribution,and basal metabolic rate(BMR)and thermal neutral zone(TNZ)are important components of the thermogenic process in animals.Furthermore,the cytochrome c oxidase 1(COX1)gene has become a subject of particular interest due to its high degree of sequence conservation,stable evolutionary rate,and rare insertions/deletions.Method:The present study selected 29 species of Rodentia and 20 species of Chiroptera.The present study employed the statistical software SPSS(27.0 Chinese version)and Origin(2024 Chinese version)software to conduct correlation analyses on a variety of biological and ecological variables.These variables included body weight,BMR,TNZ,upper thermal neutral zone,lower thermal neutral zone(LTNZ),litter size,and dietary patterns of species from different latitudes.Furthermore,we conducted a series of phylogenetic tree analyses on COX1 protein.Results:As the geographic location of a species increases in latitude,there is an observed upward trend in both body mass and BMR of rodent species.Rodentia and Chiroptera species have been observed to exhibit a decrease in LTNZ and an expansion of TNZ.With regard to dietary habits,Rodentia species are predominantly phytophagous or omnivorous.Omnivorous species exhibit a marked tendency to produce larger litters in comparison to their herbivorous counterparts.Chiroptera species exhibit a diverse dietary range,including phytophagous,carnivorous,and omnivorous species.However,no correlation was observed between dietary differences and litter size.Phylogenetic analysis of the COX1 protein subsequently demonstrated that these two species groups share a monophyletic origin.Conclusion:The present study suggests that the selected Rodentia and Chiroptera species adapt to high-latitude environments by lowering the LTNZ and widening the TNZ.Furthermore,an upward trend has been observed in the body mass and BMR of high-latitude Rodentia.Phylogenetic analysis of the COX1 protein across various taxonomic groups substantiates the efficacy of this gene for species identification.Integrating physiological phenotypes with COX1 protein molecular evidence,this study provides a reference framework for the multidimensional mechanisms of mammalian latitudinal adaptation.
基金supported by the National Natural Science Foundation of China(32130013,32070434)the National Key Research and Development Program of China(2022YFC2601601)+1 种基金the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK05010112,2019QZKK0304-02)Hainan Tropical Rainforest Conservation Research Project,ZDYF2023RDYL01(supported by the Hainan Institute of National Park,HINP,KY-24ZK02).
文摘Understanding the genetic diversity–area relationship(GAR)is essential for comprehending how species adapt to environmental changes,as genetic diversity is an indicator of a species’adaptive potential.Variation in environmental adaptation capacity exists among species and animal taxa with different distribution areas,highlighting the importance of understanding the GAR.To obtain a more comprehensive understanding of the GAR in terrestrial vertebrates,we assessed both haplotype diversity–area and nucleotide diversity–area relationships using 25,453 cytochrome c oxidase subunit I(COI)sequences from 142 amphibian species,574 bird species,and 342 mammal species.We found that both measures of genetic diversity increased with species range size across major animal groups.Nevertheless,the GAR did not differ among animal groups,while haplotype diversity performed better than nucleotide diversity in profiling the GAR,as indicated by higher R2 values.The difference in the modeling fit may stem from the distinct biological and mathematical significance of nucleotide diversity and haplotype diversity.These results suggest that the GAR follows similar rules among different animal taxa.Furthermore,haplotype diversity may serve as a more reliable indicator for assessing the potential effects of area size changes on animal populations and provide better guidance for conserving genetic diversity.
基金supplied by the National Science Funds for Distinguished Young Scientists (30925008) to F.M. Leithe NSFC program (J0930004) to C.Y. Dai+3 种基金the International Cooperation between the Chinese and Slovak Academy of Sciences to F.M. Lei and A. Kritínthe Program of the Ministry of Education of China (206148) to Z.M. Lianthe Program of the Education Department of the Shaanxi Provincial Government (2010JK909)the Program of the Science and Technology Department of Yan’an,China (YAKY200701) to X. Lei
文摘Members of the passerine family Sylviidae are distributed widely around the Old World and the phylogenetic relationships of many species still remain controversial.In this study,we investigated the phylogeny and relationships among 37 species of 11 genera by analyzing DNA sequences obtained from the complete mitochondrial cytochrome b (cyt b) and partial cytochrome oxidase I (COI) genes.The data were analyzed by maximum-likelihood analysis and Bayesian inference.The results demonstrate that the current genus Phylloscopus is non-monophyletic,while Seicercus is synonymized with Phylloscopus or is a group within the genus Phylloscopus.We may conclude that within Phylloscopus there are close relations between P.magnirostris and P.borealis;among P.proregulus,P.yunnanensis,P.pulcher and P.maculipennis,as well as among P.occisinensis,P.schwarzi,P.armandii,P.collybita and P.fuscatus.Monophyly of the genus Cettia could not be corroborated;it is closely related to the genera Abroscopus and Tesia.However monophyly of Acrocephalus is supported.Furthermore,the close relationships between Sylvia and Zosterops and among Prinia,Orthotomus and Cisticola are also supported.
