OBJECTIVE: To investigate the efficacy of Ciji Hua'ai Baosheng formula(CHBF) on microvessel density(MVD) and vascular endothelial growth factor(VEGF), kinase insert domain-containing receptor(KDR) and basic fibrob...OBJECTIVE: To investigate the efficacy of Ciji Hua'ai Baosheng formula(CHBF) on microvessel density(MVD) and vascular endothelial growth factor(VEGF), kinase insert domain-containing receptor(KDR) and basic fibroblast growth factor(b FGF) expression in serum and tumor tissue of mice receiving chemotherapy for the treatment of H22 hepatocellular carcinoma.METHODS: Sixty Kunming mice were injected subcutaneously with H22 hepatoma carcinoma cell suspensions into the right anterior armpit. Seven days later, all transplanted tumor were formed and the mice were intraperitoneally injected 200 mg/kg cytoxan(CTX) to establish the models of tumor-bearing mouse chemotherapy, then they were randomly divided into model group, continuing CTX chemotherapy group(CTX group), and three CHBF(117, 58.5 and 29.25 g/kg) groups. After ten days of treatments, histology was observed, contents of VEGF, KDR and b FGF in serum and tumor tissue were measured by enzyme-linked immunosorbent assay(ELISA), VEGF and b FGF protein expression and MVD tagged by CD34 were detected by immunohistochemisty.RESULTS: MVD in CHBF(117, 58.5 g/kg) and CTX groups was significantly lower than that in model group(P < 0.01); expressions of VEGF, KDR and b FGF in serum and tumor tissue in CHBF(117 g/kg)group were less than those in model group(P <0.05; P < 0.01); the expressions of MVD, VEGF and b FGF in tumor tissue of CHBF(117 g/kg) groupwere also less than those in CTX group(P < 0.05;P < 0.01).CONCLUSION: CHBF can effectively reduce the expression of VEGF, KDR and b FGF in serum and tumor tissue, and decrease MVD and delay tumor progression.展开更多
Purpose: To evaluate short-term effects of single photodynamic therapy (PDT) for age-related macular degeneration (AMD) accompanied with choroidal neovascularization (CNV).Methods: We analyzed retrospectively the effe...Purpose: To evaluate short-term effects of single photodynamic therapy (PDT) for age-related macular degeneration (AMD) accompanied with choroidal neovascularization (CNV).Methods: We analyzed retrospectively the effects of single PDT for 20 patients (20 eyes) with CNV caused by AMD. Corrected visual acuity, fluorescein angiography (FA) and optic coherence tomography (OCT) were examined before and after PDT. All patients were followed up at least 3 months. Results: At the end of 3-month follow-up, 5 eyes had vision progress, 15 eyes had vision stable and no eye had vision deterioration. Fluorescein angiography one week post PDT showed cessation of fluorescein leakage in 8 eyes with predominant classic CNV, and reduction of fluorescein leakage in 12 eyes with minimal classic CNV or occult CNV without classic component. At the 3-month following PDT fluorescein angiography showed fluorescein leakage reappeared in 4 of 8 eyes with predominant classic CNV. Among 12 eyes with minimal classic CNV or occult CNV without classic component, 9 eyes showed decreased or unchanged fluorescein leakage, 3 eyes had a progression of fluorescein leakage. Optic coherence tomography showed obvious recovery of serous sensory retinal detachment after PDT. Conclusion: PDT may occlude or inhibit CNV caused by AMD in short term. No obvious side effects were noticed.展开更多
A new method of light-powered dissipative supra-molecular polymerization is established,in which supramolecular polymerization is implemented in the far-from-equilibrium state.A bifunctional mono-mer containing two vi...A new method of light-powered dissipative supra-molecular polymerization is established,in which supramolecular polymerization is implemented in the far-from-equilibrium state.A bifunctional mono-mer containing two viologen moieties was designed.Upon inputting energy by light,the sys-tem was driven far from equilibrium,and the mono-mers were photoreduced and activated to form supramolecular polymers driven by 2∶1 host–vip complexation of the viologen cation radical and cucurbit[8]uril.As the system returned to equilibri-um,the supramolecular polymers depolymerized spontaneously by air oxidation.This method works in both linear and in cross-linked supramolecular polymerization.The strategy of light-powered dis-sipative supramolecular polymerization is anticipat-ed to have potential in the fabrication of functional supramolecular materials,especially in creating novel“living”materials.展开更多
A hydrolyte of magnesium 7 methoxyl 4′ hydroxylisoflavone 3′ sulfonate ([Mg(H 2O) 6](C 16 H 11 O 4SO 3) 2·10H 2O) was synthesized and its crystal structure was determined by X ray diffractio...A hydrolyte of magnesium 7 methoxyl 4′ hydroxylisoflavone 3′ sulfonate ([Mg(H 2O) 6](C 16 H 11 O 4SO 3) 2·10H 2O) was synthesized and its crystal structure was determined by X ray diffraction analyses. The crystal belongs to triclinic crystal system, space group P ī with cell constants a =0 7359(1) nm, b =0 9935(1) nm, c = 1 6335(2) nm, α =91 16(1)°, β =92 07(1)°, γ =106 53(1)°, V =1 1436(2) nm 3 and Z =1. The results show that the title compound consists of [Mg(H 2O) 6] 2+ , C 16 H 11 O 4SO - 3 and H 2O. Mg(II) is located at the symmetry center of the title compound. [Mg(H 2O) 6] 2+ , C 16 H 11 O 4SO - 3 and H 2O form fourteen kinds of hydrogen bonds. The distance of 0 351 nm between parallel isoflavone rings shows a π π stacking interaction. The hydrogen bonds and π π stacking interaction lead to supramolecular formation with three dimensional network structure. The title compound was also characterized by IR and 1H NMR spectra.展开更多
基金the National Natural Science Foundation of China(From Regulating Pathological Angiogenesis and the Hepatic Fibrosis Way to Compare the Effects and Mechanisms between Taohongsiwu Decoction and its Absorbed Bioactive Compound,No.81202659)the Xiamen Science and Technology Key Program Plan Grant(Prevention and Treatment Mechanism Study of Traditional Chinese Medicine on Common Digestive Tract Cancer of Fujian and Taiwan,No.3502Z20100006)+2 种基金the Xiamen Science and Technology Program Plan Grant(the Development and Application and Basic Research of Ciji Hua'ai Baosheng Soluble Granules,No.3502Z20153027)the Natural Science Foundation of Fujian(China)(Negative Effect of Safflower Ingredient and Taohong formula on Mechanism of Pathological Angiogenesis and PI3K-Akt Signaling,No.2014J01373)the Scientific Research Start Foundation for New Teacher of Xiamen University(Correlated Mechanism Study of Safflower Ingredient Regulating Digestive Tract Tumor,No.ZK1014)
文摘OBJECTIVE: To investigate the efficacy of Ciji Hua'ai Baosheng formula(CHBF) on microvessel density(MVD) and vascular endothelial growth factor(VEGF), kinase insert domain-containing receptor(KDR) and basic fibroblast growth factor(b FGF) expression in serum and tumor tissue of mice receiving chemotherapy for the treatment of H22 hepatocellular carcinoma.METHODS: Sixty Kunming mice were injected subcutaneously with H22 hepatoma carcinoma cell suspensions into the right anterior armpit. Seven days later, all transplanted tumor were formed and the mice were intraperitoneally injected 200 mg/kg cytoxan(CTX) to establish the models of tumor-bearing mouse chemotherapy, then they were randomly divided into model group, continuing CTX chemotherapy group(CTX group), and three CHBF(117, 58.5 and 29.25 g/kg) groups. After ten days of treatments, histology was observed, contents of VEGF, KDR and b FGF in serum and tumor tissue were measured by enzyme-linked immunosorbent assay(ELISA), VEGF and b FGF protein expression and MVD tagged by CD34 were detected by immunohistochemisty.RESULTS: MVD in CHBF(117, 58.5 g/kg) and CTX groups was significantly lower than that in model group(P < 0.01); expressions of VEGF, KDR and b FGF in serum and tumor tissue in CHBF(117 g/kg)group were less than those in model group(P <0.05; P < 0.01); the expressions of MVD, VEGF and b FGF in tumor tissue of CHBF(117 g/kg) groupwere also less than those in CTX group(P < 0.05;P < 0.01).CONCLUSION: CHBF can effectively reduce the expression of VEGF, KDR and b FGF in serum and tumor tissue, and decrease MVD and delay tumor progression.
文摘Purpose: To evaluate short-term effects of single photodynamic therapy (PDT) for age-related macular degeneration (AMD) accompanied with choroidal neovascularization (CNV).Methods: We analyzed retrospectively the effects of single PDT for 20 patients (20 eyes) with CNV caused by AMD. Corrected visual acuity, fluorescein angiography (FA) and optic coherence tomography (OCT) were examined before and after PDT. All patients were followed up at least 3 months. Results: At the end of 3-month follow-up, 5 eyes had vision progress, 15 eyes had vision stable and no eye had vision deterioration. Fluorescein angiography one week post PDT showed cessation of fluorescein leakage in 8 eyes with predominant classic CNV, and reduction of fluorescein leakage in 12 eyes with minimal classic CNV or occult CNV without classic component. At the 3-month following PDT fluorescein angiography showed fluorescein leakage reappeared in 4 of 8 eyes with predominant classic CNV. Among 12 eyes with minimal classic CNV or occult CNV without classic component, 9 eyes showed decreased or unchanged fluorescein leakage, 3 eyes had a progression of fluorescein leakage. Optic coherence tomography showed obvious recovery of serous sensory retinal detachment after PDT. Conclusion: PDT may occlude or inhibit CNV caused by AMD in short term. No obvious side effects were noticed.
基金This work is supported financially by the National Natural Science Foundation of China(21434004,21890731,21821001,and 91527000)P.Z.is supported by the Na-tional Natural Science Foundation of China(21771103)the Natural Science Foundation of Jiangsu Province(BK20160639)。
文摘A new method of light-powered dissipative supra-molecular polymerization is established,in which supramolecular polymerization is implemented in the far-from-equilibrium state.A bifunctional mono-mer containing two viologen moieties was designed.Upon inputting energy by light,the sys-tem was driven far from equilibrium,and the mono-mers were photoreduced and activated to form supramolecular polymers driven by 2∶1 host–vip complexation of the viologen cation radical and cucurbit[8]uril.As the system returned to equilibri-um,the supramolecular polymers depolymerized spontaneously by air oxidation.This method works in both linear and in cross-linked supramolecular polymerization.The strategy of light-powered dis-sipative supramolecular polymerization is anticipat-ed to have potential in the fabrication of functional supramolecular materials,especially in creating novel“living”materials.
基金ProjectsupportedbytheNaturalScienceFoundationofShaanxiProvince (No .2 0 0 1K11 G5 )
文摘A hydrolyte of magnesium 7 methoxyl 4′ hydroxylisoflavone 3′ sulfonate ([Mg(H 2O) 6](C 16 H 11 O 4SO 3) 2·10H 2O) was synthesized and its crystal structure was determined by X ray diffraction analyses. The crystal belongs to triclinic crystal system, space group P ī with cell constants a =0 7359(1) nm, b =0 9935(1) nm, c = 1 6335(2) nm, α =91 16(1)°, β =92 07(1)°, γ =106 53(1)°, V =1 1436(2) nm 3 and Z =1. The results show that the title compound consists of [Mg(H 2O) 6] 2+ , C 16 H 11 O 4SO - 3 and H 2O. Mg(II) is located at the symmetry center of the title compound. [Mg(H 2O) 6] 2+ , C 16 H 11 O 4SO - 3 and H 2O form fourteen kinds of hydrogen bonds. The distance of 0 351 nm between parallel isoflavone rings shows a π π stacking interaction. The hydrogen bonds and π π stacking interaction lead to supramolecular formation with three dimensional network structure. The title compound was also characterized by IR and 1H NMR spectra.
