Ti(C,N)基金属陶瓷显微组织及相界面较复杂,需要一种简便、精确的定量金相方法来辅助研究.采用功能强大的Gatan Digital Micrograph软件对不同纳米添加量Ti(C,N)基金属陶瓷显微组织进行背底消除、滤波、二值化及图像形态学处理,分析可...Ti(C,N)基金属陶瓷显微组织及相界面较复杂,需要一种简便、精确的定量金相方法来辅助研究.采用功能强大的Gatan Digital Micrograph软件对不同纳米添加量Ti(C,N)基金属陶瓷显微组织进行背底消除、滤波、二值化及图像形态学处理,分析可得到该金相图中的一些金相特征参数.对不同纳米添加量Ti(C,N)基金属陶瓷中硬质相进行了综合分析,结果表明,利用Gatan Digi-tal Micrograph软件实现Ti(C,N)基金属陶瓷显微组织的分析测量是完全可行的,能有效地提高工作效率及得到直观、准确的结果,也可定量地得到随纳米粉添加量的不断增加,硬质相颗粒的总体积不断减少,平均粒径也在不断减小.展开更多
Chaperonins, a class of molecular chaperones, are oligomeric complexes acting as a protein-folding chamber in an ATP-dependent manner. Chaperonins have been classifed
Morphology of hydraulic fracture surface has significant effects on oil and gas flow,proppant migration and fracture closure,which plays an important role in oil and gas fracturing stimulation.In this paper,we analyze...Morphology of hydraulic fracture surface has significant effects on oil and gas flow,proppant migration and fracture closure,which plays an important role in oil and gas fracturing stimulation.In this paper,we analyzed the fracture surface characteristics induced by supercritical carbon dioxide(SC-CO_(2))and water in open-hole and perforation completion conditions under triaxial stresses.A simple calculation method was proposed to quantitatively analyze the fracture surface area and roughness in macro-level based on three-dimensional(3D)scanning data.In micro-level,scanning electron micrograph(SEM)was used to analyze the features of fracture surface.The results showed that the surface area of the induced fracture increases with perforation angle for both SC-CO_(2)and water fracturing,and the surface area of SC-CO_(2)-induced fracture is 6.49%e58.57%larger than that of water-induced fracture.The fractal dimension and surface roughness of water-induced fractures increase with the increase in perforation angle,while those of SC-CO_(2)-induced fractures decrease with the increasing perforation angle.A considerable number of microcracks and particle peeling pits can be observed on SC-CO_(2)-induced fracture surface while there are more flat particle surfaces in water-induced fracture surface through SEM images,indicating that fractures tend to propagate along the boundary of the particle for SC-CO_(2)fracturing while water-induced fractures prefer to cut through particles.These findings are of great significance for analyzing fracture mechanism and evaluating fracturing stimulation performance.展开更多
This paper deals with detailed corrosion analysis of explanted devices. The study of total 6 different types of orthopedic metallic implant was carried out after collecting the clinical report from the doctors, who pe...This paper deals with detailed corrosion analysis of explanted devices. The study of total 6 different types of orthopedic metallic implant was carried out after collecting the clinical report from the doctors, who performed these implantations. The clinical report covered the purpose of implantation, body part where implantation was done, and physiological reasons of removal of implant. The metallurgical investigation to study corrosion and any other mechanical damage to the implant surface during their service period was done using the Scanning Electron Micrography. SEM presented in this paper reveals the presence of in-vitro corrosion and mechanical damage as well, which are corroborating well with clinical reports.展开更多
Three-dimensional(3 D)reconstruction of icosahedral viruses has played a crucial role in the development of cryoelectron microscopy single-particle reconstruction,with many cryo-electron microscopy techniques first es...Three-dimensional(3 D)reconstruction of icosahedral viruses has played a crucial role in the development of cryoelectron microscopy single-particle reconstruction,with many cryo-electron microscopy techniques first established for structural studies of icosahedral viruses,owing to their high symmetry and large mass.This review summarizes the computational methods for icosahedral and symmetry-mismatch reconstruction of viruses,as well as the likely challenges and bottlenecks in virus reconstruction,such as symmetry mismatch reconstruction,contrast transformation function(CTF)correction,and particle distortion.展开更多
Recent technical breakthroughs in cryo-electron microscopy（cryo-EM） revolutionized structural biology, which led to the 2017 Nobel Prize in chemistry being awarded to three scientists, Jacques Dubochet, Joachim Fran...Recent technical breakthroughs in cryo-electron microscopy（cryo-EM） revolutionized structural biology, which led to the 2017 Nobel Prize in chemistry being awarded to three scientists, Jacques Dubochet, Joachim Frank, and Richard Henderson, who made groundbreaking contributions to the development of cryo-EM. In this review, I will give a comprehensive review of the developmental history of cryo-EM, the technical aspects of the breakthrough in cryo-EM leading to the structural biology revolution, including electron microscopy, image recording devices and image processing algorithms,and the major scientific achievements by Chinese researchers employing cryo-EM, covering protein complexes involved in or related to gene expression and regulation, protein synthesis and degradation, membrane proteins, immunity, and viruses.Finally, I will give a perspective outlook on the development of cryo-EM in the future.展开更多
Cryo-electron microscopic images of biological molecules usually have high noise and low contrast.It is essential to suppress noise and enhance contrast in order to recognize particles in the images.A local adaptive d...Cryo-electron microscopic images of biological molecules usually have high noise and low contrast.It is essential to suppress noise and enhance contrast in order to recognize particles in the images.A local adaptive denoising method based on non-local means filter[1],which can preserve signal details and simultaneously suppress noise for cryo-electron microscopy data is presented.This approach greatly suppress the noise and enhances the contrast on simulated image data compared to other widely used denoising methods[2-4].展开更多
Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,startin...Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,starting from two baculoviral capsid proteins,we screened protein variants for the in vitro assembly of various nanotubes and nanosheets.These high-order assemblies were structurally characterized by cryo-electron microscopy techniques.Interfacial analysis of three groups of PNTs revealed that helical heterogeneity is largely the result of the redundancy of p2 symmetry-related contacting interfaces.The assembled nanosheets showed similar interfacial networks to their nanotubular counterparts.In addition,foreign macromolecules could be efficiently displayed on the sizecontrollable double-layered nanosheets.This study sheds light on the rational design of flexible nanosheets,and it also provides novel 2D protein scaffolds for developing biocompatible materials.展开更多
文摘Ti(C,N)基金属陶瓷显微组织及相界面较复杂,需要一种简便、精确的定量金相方法来辅助研究.采用功能强大的Gatan Digital Micrograph软件对不同纳米添加量Ti(C,N)基金属陶瓷显微组织进行背底消除、滤波、二值化及图像形态学处理,分析可得到该金相图中的一些金相特征参数.对不同纳米添加量Ti(C,N)基金属陶瓷中硬质相进行了综合分析,结果表明,利用Gatan Digi-tal Micrograph软件实现Ti(C,N)基金属陶瓷显微组织的分析测量是完全可行的,能有效地提高工作效率及得到直观、准确的结果,也可定量地得到随纳米粉添加量的不断增加,硬质相颗粒的总体积不断减少,平均粒径也在不断减小.
文摘Chaperonins, a class of molecular chaperones, are oligomeric complexes acting as a protein-folding chamber in an ATP-dependent manner. Chaperonins have been classifed
基金National Natural Science Foundation of China(Grant No.51804318)the China Postdoctoral Science Foundation Founded Project(Grant No.2019M650963)National Key Basic Research and Development Program of China(Grant No.2014CB239203).
文摘Morphology of hydraulic fracture surface has significant effects on oil and gas flow,proppant migration and fracture closure,which plays an important role in oil and gas fracturing stimulation.In this paper,we analyzed the fracture surface characteristics induced by supercritical carbon dioxide(SC-CO_(2))and water in open-hole and perforation completion conditions under triaxial stresses.A simple calculation method was proposed to quantitatively analyze the fracture surface area and roughness in macro-level based on three-dimensional(3D)scanning data.In micro-level,scanning electron micrograph(SEM)was used to analyze the features of fracture surface.The results showed that the surface area of the induced fracture increases with perforation angle for both SC-CO_(2)and water fracturing,and the surface area of SC-CO_(2)-induced fracture is 6.49%e58.57%larger than that of water-induced fracture.The fractal dimension and surface roughness of water-induced fractures increase with the increase in perforation angle,while those of SC-CO_(2)-induced fractures decrease with the increasing perforation angle.A considerable number of microcracks and particle peeling pits can be observed on SC-CO_(2)-induced fracture surface while there are more flat particle surfaces in water-induced fracture surface through SEM images,indicating that fractures tend to propagate along the boundary of the particle for SC-CO_(2)fracturing while water-induced fractures prefer to cut through particles.These findings are of great significance for analyzing fracture mechanism and evaluating fracturing stimulation performance.
文摘This paper deals with detailed corrosion analysis of explanted devices. The study of total 6 different types of orthopedic metallic implant was carried out after collecting the clinical report from the doctors, who performed these implantations. The clinical report covered the purpose of implantation, body part where implantation was done, and physiological reasons of removal of implant. The metallurgical investigation to study corrosion and any other mechanical damage to the implant surface during their service period was done using the Scanning Electron Micrography. SEM presented in this paper reveals the presence of in-vitro corrosion and mechanical damage as well, which are corroborating well with clinical reports.
基金Project supported by the National Key R&D Program of China(Grant No.2016YFA0501100)the National Natural Science Foundation of China(Grant Nos.91530321,31570742,and 31570727)Science and Technology Planning Project of Hunan Province,China(Grant No.2017RS3033)
文摘Three-dimensional(3 D)reconstruction of icosahedral viruses has played a crucial role in the development of cryoelectron microscopy single-particle reconstruction,with many cryo-electron microscopy techniques first established for structural studies of icosahedral viruses,owing to their high symmetry and large mass.This review summarizes the computational methods for icosahedral and symmetry-mismatch reconstruction of viruses,as well as the likely challenges and bottlenecks in virus reconstruction,such as symmetry mismatch reconstruction,contrast transformation function(CTF)correction,and particle distortion.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFA0504700)the National Natural Science Foundation of China(Grant Nos.31570732 and 31770785)
文摘Recent technical breakthroughs in cryo-electron microscopy（cryo-EM） revolutionized structural biology, which led to the 2017 Nobel Prize in chemistry being awarded to three scientists, Jacques Dubochet, Joachim Frank, and Richard Henderson, who made groundbreaking contributions to the development of cryo-EM. In this review, I will give a comprehensive review of the developmental history of cryo-EM, the technical aspects of the breakthrough in cryo-EM leading to the structural biology revolution, including electron microscopy, image recording devices and image processing algorithms,and the major scientific achievements by Chinese researchers employing cryo-EM, covering protein complexes involved in or related to gene expression and regulation, protein synthesis and degradation, membrane proteins, immunity, and viruses.Finally, I will give a perspective outlook on the development of cryo-EM in the future.
文摘Cryo-electron microscopic images of biological molecules usually have high noise and low contrast.It is essential to suppress noise and enhance contrast in order to recognize particles in the images.A local adaptive denoising method based on non-local means filter[1],which can preserve signal details and simultaneously suppress noise for cryo-electron microscopy data is presented.This approach greatly suppress the noise and enhances the contrast on simulated image data compared to other widely used denoising methods[2-4].
基金supported by the National Key Research and Development Program of China(2022YFC2303300 to S.C.)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0490000 to S.C.)+1 种基金the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2022341 to G.R.)the Major Project of the Guangzhou National Laboratory(GZNL2024A01010 to G.R.).
文摘Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,starting from two baculoviral capsid proteins,we screened protein variants for the in vitro assembly of various nanotubes and nanosheets.These high-order assemblies were structurally characterized by cryo-electron microscopy techniques.Interfacial analysis of three groups of PNTs revealed that helical heterogeneity is largely the result of the redundancy of p2 symmetry-related contacting interfaces.The assembled nanosheets showed similar interfacial networks to their nanotubular counterparts.In addition,foreign macromolecules could be efficiently displayed on the sizecontrollable double-layered nanosheets.This study sheds light on the rational design of flexible nanosheets,and it also provides novel 2D protein scaffolds for developing biocompatible materials.
