Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide...Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide and C.crustorum LBK-CCMXJ1001(LBK1001)in Caenorhabditis elegans.Our findings revealed that fraction 1 of C.deserticola polysaccharide(CD-1)enhanced the proliferation and antioxidant properties of LBK1001 in vitro.LBK1001 significantly increased the number of head swinging,frequency of pharyngeal pumps,and the activities of antioxidant enzymes,including SOD,CAT,and GSH-px,and reduced the levels of oxidized free radicals,lipofuscin,and MDA in C.elegans.Furthermore,the LBK1001-CD-1 complex exhibited greater bioactive effects than LBK1001 alone.Both LBK1001 and the LBK1001-CD-1 complex decreased the expression of the daf-2 and akt-1 genes while increasing sod-3,daf-16,hsp-16.2,and skn-1 in C.elegans using qPCR analysis.Correlation analysis indicated that the expressions of daf-2 and akt-1 were negatively correlated with SOD,GSH-px,and CAT,but positively correlated with MDA,ROS,and lipofuscin.Conversely,daf-16,hsp-16.2,and skn-1 exhibited an inverse relationship with daf-2 and akt-1.This was closely associated with the reversal of aging symptoms in C.elegans.The LBK1001-CD-1 complex demonstrated enhanced effects on lifespan extension and antioxidant capacity in C.elegans compared to LBK1001 alone,and it prolonged lifespan by regulating the anti-oxidation system and the expressions of genes related to aging.This indicated that the LBK1001-CD-1 complex modulated the antioxidant defense system of C.elegans while scavenging peroxides,promoting motility,and delaying aging.展开更多
The role of the luxS/autoinducer-2(AI-2)quorum sensing system in probiotic efficacy is controversial.This study investigated the therapeutic potential of a luxS-deficient mutant(△luxS)of Companilactobacillus crustoru...The role of the luxS/autoinducer-2(AI-2)quorum sensing system in probiotic efficacy is controversial.This study investigated the therapeutic potential of a luxS-deficient mutant(△luxS)of Companilactobacillus crustorum MN047 in a dextran sulfate sodium(DSS)-induced colitis mouse model.Unexpectedly,the△luxS mutant developed a pronounced auto-aggregation phenotype(>90%)and significantly enhanced bile salt tolerance in vitro.Mechanistically,these physical adaptations were driven by the redirection of carbon flux toward exopo-lysaccharide biosynthesis via the upregulation of specific phosphotransferase systems(PTS).In vivo,these traits translated into superior therapeutic efficacy compared to the wild-type.The mutant effectively alleviated colitis symptoms and restored intestinal barrier integrity.Furthermore,the mutant reshaped the gut microbiota,spe-cifically enriching short-chain fatty acid(SCFA)producers(Bacteroides and Marinifilaceae),which elevated anti-inflammatory metabolites.These findings indicate that targeted modulation of the LuxS/AI-2 system could be a viable strategy for engineering next-generation probiotics with enhanced physiological resilience.展开更多
基金supported by the Talent Program“Tianchi Talent(Young Doctor)”in Xinjiang Uygur Autonomous Regionthe Corps Guiding Science and Technology program projects(grant number 2023ZD087)+2 种基金the National Natural Science Foundation of China(grant number 32060548)Key Project of Karamay City in Xinjiang(2023kqzdyf0026)the Xinjiang Uygur Autonomous Region Regional Collaborative Innovation Program(grant number 2022E01028).
文摘Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide and C.crustorum LBK-CCMXJ1001(LBK1001)in Caenorhabditis elegans.Our findings revealed that fraction 1 of C.deserticola polysaccharide(CD-1)enhanced the proliferation and antioxidant properties of LBK1001 in vitro.LBK1001 significantly increased the number of head swinging,frequency of pharyngeal pumps,and the activities of antioxidant enzymes,including SOD,CAT,and GSH-px,and reduced the levels of oxidized free radicals,lipofuscin,and MDA in C.elegans.Furthermore,the LBK1001-CD-1 complex exhibited greater bioactive effects than LBK1001 alone.Both LBK1001 and the LBK1001-CD-1 complex decreased the expression of the daf-2 and akt-1 genes while increasing sod-3,daf-16,hsp-16.2,and skn-1 in C.elegans using qPCR analysis.Correlation analysis indicated that the expressions of daf-2 and akt-1 were negatively correlated with SOD,GSH-px,and CAT,but positively correlated with MDA,ROS,and lipofuscin.Conversely,daf-16,hsp-16.2,and skn-1 exhibited an inverse relationship with daf-2 and akt-1.This was closely associated with the reversal of aging symptoms in C.elegans.The LBK1001-CD-1 complex demonstrated enhanced effects on lifespan extension and antioxidant capacity in C.elegans compared to LBK1001 alone,and it prolonged lifespan by regulating the anti-oxidation system and the expressions of genes related to aging.This indicated that the LBK1001-CD-1 complex modulated the antioxidant defense system of C.elegans while scavenging peroxides,promoting motility,and delaying aging.
基金supported by the National Natural Science Foundation of China(No.32302260)the Scientific Research Program Funded by Shaanxi Provincial Education Department(No.25JC008)+1 种基金the Shaanxi Province Postdoctoral Science Foundation(No.2025BSHEDZZ031)Shandong Province postdoctoral Fund project(No.SDCX-ZG-202400136).
文摘The role of the luxS/autoinducer-2(AI-2)quorum sensing system in probiotic efficacy is controversial.This study investigated the therapeutic potential of a luxS-deficient mutant(△luxS)of Companilactobacillus crustorum MN047 in a dextran sulfate sodium(DSS)-induced colitis mouse model.Unexpectedly,the△luxS mutant developed a pronounced auto-aggregation phenotype(>90%)and significantly enhanced bile salt tolerance in vitro.Mechanistically,these physical adaptations were driven by the redirection of carbon flux toward exopo-lysaccharide biosynthesis via the upregulation of specific phosphotransferase systems(PTS).In vivo,these traits translated into superior therapeutic efficacy compared to the wild-type.The mutant effectively alleviated colitis symptoms and restored intestinal barrier integrity.Furthermore,the mutant reshaped the gut microbiota,spe-cifically enriching short-chain fatty acid(SCFA)producers(Bacteroides and Marinifilaceae),which elevated anti-inflammatory metabolites.These findings indicate that targeted modulation of the LuxS/AI-2 system could be a viable strategy for engineering next-generation probiotics with enhanced physiological resilience.
