Background:Crossbow medicine needle is a traditional external therapy.However,acupuncture pain,inaccurate administration,secondary infection,and poor patient compliance limit the application of specialty therapies.Sin...Background:Crossbow medicine needle is a traditional external therapy.However,acupuncture pain,inaccurate administration,secondary infection,and poor patient compliance limit the application of specialty therapies.Since the most effective components of crossbow medicine are lipid-soluble,the crossbow medicine needle was prepared as an innovative O/W-type microemulsion to solve these problems.In this study,the crossbow medicine microemulsion preparation method was established and its quality was evaluated.Methods:A single factor and pseudoternary phase diagram,combined with a D-optimal mixture design,optimized the formulation of crossbow medicine microemulsion through particle size,polydispersity index,zeta potential,microstructure,and contents of benzoyl neoaconitine,benzoyl hypoaconitine,neoaconitine,periplocin,and methyl salicylate to evaluate the final prescription.Results:The best prescription for preparing crossbow medicine microemulsion is 0.1500 g crossbow medicine dry extract,0.1500 g isopropyl myristate,0.8625 g polyoxyethylene castor oil-35,0.2875 g ethanol,3.700 g water,and 5.1500 g microemulsion.The prepared microemulsion is an oil-in-water type with a clear and transparent appearance.Its average particle size is 19.83±0.78 nm,average polydispersity index is 0.15±0.02,and average zeta potential is−0.51±0.1 mV.The pH value is 5.17±0.02.The microstructure of crossbow medicine microemulsion illustrated a uniform distribution of spherical oil globules.The average contents of benzoyl neoaconitine,benzoyl hypoaconitine,neoaconitine,periplocin,and methyl salicylate were 13.34,113.05,43.45,628.27 and 27.93μg/g,respectively.Conclusion:The preparation process of crossbow medicine microemulsion is reasonable and feasible.The method to determine the content is accurate and straightforward,laying the foundation for further research.展开更多
目的观察改良型弩药针疗法治疗膝关节骨性关节炎(KOA)的临床疗效。方法按随机数字法将60例KOA患者分为两组,治疗组30例用改良型弩药针疗法治疗;对照组30例用传统弩药针疗法治疗;操作部位均为膝眼(双)、阳陵泉、鹤顶及膝关节局部疼痛点,2...目的观察改良型弩药针疗法治疗膝关节骨性关节炎(KOA)的临床疗效。方法按随机数字法将60例KOA患者分为两组,治疗组30例用改良型弩药针疗法治疗;对照组30例用传统弩药针疗法治疗;操作部位均为膝眼(双)、阳陵泉、鹤顶及膝关节局部疼痛点,2 d 1次,10次为1个疗程,共治疗2个疗程。评价其治疗后和治疗3个月后疗效。结果治疗组治疗后总有效率为90.00%,高于对照组的83.33%,治疗后3个月治疗组总有效率为86.67%,高于对照组的80.0%,治疗组与对照组治疗后组间及治疗后3个月组间VAS评分比较差异有统计学意义(P<0.05)。结论改良型弩药针疗法对KOA具有很好的疗效,且见效快,疗效稳定,方便易操作,值得进一步推广。展开更多
基金This work was supported by the Projects from Guiyang University of Traditional Chinese Medicine(No.(2017)5735-27)National Engineering Research Center of Miao’s Medicines(No.2014FU125Q09)Science and Technology Department of Guizhou Province(No.GNYL(2017)008,No.(2015)4030,No.(2017)5655,and No.YJSCXJH(2020)157).
文摘Background:Crossbow medicine needle is a traditional external therapy.However,acupuncture pain,inaccurate administration,secondary infection,and poor patient compliance limit the application of specialty therapies.Since the most effective components of crossbow medicine are lipid-soluble,the crossbow medicine needle was prepared as an innovative O/W-type microemulsion to solve these problems.In this study,the crossbow medicine microemulsion preparation method was established and its quality was evaluated.Methods:A single factor and pseudoternary phase diagram,combined with a D-optimal mixture design,optimized the formulation of crossbow medicine microemulsion through particle size,polydispersity index,zeta potential,microstructure,and contents of benzoyl neoaconitine,benzoyl hypoaconitine,neoaconitine,periplocin,and methyl salicylate to evaluate the final prescription.Results:The best prescription for preparing crossbow medicine microemulsion is 0.1500 g crossbow medicine dry extract,0.1500 g isopropyl myristate,0.8625 g polyoxyethylene castor oil-35,0.2875 g ethanol,3.700 g water,and 5.1500 g microemulsion.The prepared microemulsion is an oil-in-water type with a clear and transparent appearance.Its average particle size is 19.83±0.78 nm,average polydispersity index is 0.15±0.02,and average zeta potential is−0.51±0.1 mV.The pH value is 5.17±0.02.The microstructure of crossbow medicine microemulsion illustrated a uniform distribution of spherical oil globules.The average contents of benzoyl neoaconitine,benzoyl hypoaconitine,neoaconitine,periplocin,and methyl salicylate were 13.34,113.05,43.45,628.27 and 27.93μg/g,respectively.Conclusion:The preparation process of crossbow medicine microemulsion is reasonable and feasible.The method to determine the content is accurate and straightforward,laying the foundation for further research.
文摘目的观察改良型弩药针疗法治疗膝关节骨性关节炎(KOA)的临床疗效。方法按随机数字法将60例KOA患者分为两组,治疗组30例用改良型弩药针疗法治疗;对照组30例用传统弩药针疗法治疗;操作部位均为膝眼(双)、阳陵泉、鹤顶及膝关节局部疼痛点,2 d 1次,10次为1个疗程,共治疗2个疗程。评价其治疗后和治疗3个月后疗效。结果治疗组治疗后总有效率为90.00%,高于对照组的83.33%,治疗后3个月治疗组总有效率为86.67%,高于对照组的80.0%,治疗组与对照组治疗后组间及治疗后3个月组间VAS评分比较差异有统计学意义(P<0.05)。结论改良型弩药针疗法对KOA具有很好的疗效,且见效快,疗效稳定,方便易操作,值得进一步推广。
