A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synth...A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synthetic metabolic engineering offers a method to modify and redesign metabolic pathways to increase the nutritional value of crops.We summarize recent advances in the biofortification of key nutrients including provitamin A,vitamin C,vitamin B9,iron,zinc,anthocyanins,flavonoids,and unsaturated fatty acids.We discuss the potential of multi-gene stacking,gene editing,enzyme engineering,and artificial intelligence in synthetic metabolic engineering.We propose future research directions and potential solutions centered on leveraging AI-driven systems biology,precision gene editing,enzyme engineering,agrobacterium-mediated genotype-independent transformation,and modular metabolic engineering strategies to develop next-generation nutritionally enhanced super crops and transform global food systems.展开更多
[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-cr...[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum.展开更多
Increased human and industrial activities have exacerbated the release of toxic materials and acute envi-ronmental pollution in recent times.Biochar,a carbon-rich material produced from biomass,is gaining momentum as ...Increased human and industrial activities have exacerbated the release of toxic materials and acute envi-ronmental pollution in recent times.Biochar,a carbon-rich material produced from biomass,is gaining momentum as a versatile material for attaining a sustainable environment.The study reviews the application of functionalized biochar for energy storage,environmental remediation,catalysis,and sustainable agriculture,aiming to achieve a greener future.Thedeployment of crop residues as a renewable feedstock for biochar,and their properties,compositions,modification,and functionalization techniques are also discussed.Additionally,the avenues for applying functionalized biochar to achieve a greener future,future trends and innovations,challenges,and future research directions are highlighted.Despite the limitations of scalability,ecotoxicological risks,logistical issues,lack of characterization protocols,high production costs,poor social acceptance,and inadequate policy and regulatory frameworks,functionalized biochar offers a better surface area,improved porosity,enhanced functional groups,and higher recoverability,leading to improved performance,adsorption capacity,biodegradability,and applications in specialized fields.Future research should prioritize standardization,scalability,cost reduction strategies,expansion of application areas,integration of emerging tools such as artificial intelligence and predictive modeling,and the development of policy and regulatory frameworks,ensuring that biochar’s full potential is harnessed effectively to support a low-carbon,resource-efficient future and global sustainability goals.展开更多
Plant growth-promoting bacteria(PGPBs)can promote plant growth and improve crop yield.They can induce plant systemic resistance to resist biotic and abiotic stresses.In recent years,with the development of green ecolo...Plant growth-promoting bacteria(PGPBs)can promote plant growth and improve crop yield.They can induce plant systemic resistance to resist biotic and abiotic stresses.In recent years,with the development of green ecological agriculture,new biological fertilizers such as microbial inocula and microbial fertilizers based on PGPBs have been gradually applied in crop planting.Based on plant growth promotion and disease control,the application progress of PGPBs in crops from the aspects of growth promotion mechanism,growth promotion effect,resistance to biological and abiotic stresses were discussed,aiming to provide reference for the relevant research and application of PGPBs in crops.展开更多
The functional diversity of rhizosphere microflora which is also known as the “microbial community” is a sensitive indicator of soil quality subject to the type of winter cover crop and straw returning.In order to e...The functional diversity of rhizosphere microflora which is also known as the “microbial community” is a sensitive indicator of soil quality subject to the type of winter cover crop and straw returning.In order to evaluate the effects of different winter cover crops and returning patterns on the functional diversity of rhizosphere microflora in double-crop rice paddies,we designed five winter cover crops and straw returning combinations to analyze their effects on the functional diversity of rhizosphere microflora in rice paddies: ryegrass (Lolium multiflorumL.)–double-crop rice (Ry),milk vetch (Astragalus sinicus L.)–double-crop rice (Mv),Rape (Brassica napus L.)–Double-crop rice (Ra), Potato (Solanum tuberosum L.)–double-crop rice (Po),and winter fallow–doublecrop rice (CK,the control).In this paper,the average well color development (AWCD) in Biolog-GN plates indicated the capacity for carbon utilization by the rhizosphere microbial community.We analyzed the rhizosphere microbial community functional diversity of the paddy soils with the above five treatments by using the Biolog-GN system.The results showed that applications of winter cover crop and straw returning caused high increases in AWCD compared with CK,and the AWCD values for samples with Po treatment was greater than those with Ry and CK treatments at the early and late rice maturity stages. It was concluded that applications of winter cover crop and straw returning can cause changes in the carbon utilization efficiency of rhizosphere microflora.There were differences in the genetic diversity of the rhizosphere microflora among different treatments at the maturity stage of early and late rice.The richness,Shannon,and McIntosh Index under different winter cover crop and straw returning treatments were significantly different.The highest indexes were observed in the Po treatment and the lowest in the CK at the maturity stage of early and late rice.The richness, Shannon,and McIntosh Index under different treatments ranked in descending order is as follows: Po>Ra>Mv>Ry>CK.Principal Component Analysis (PCA) of substrate reactions were conducted in this research.The results indicated that the pattern of carbon source utilization varied with winter cover crop treatments,and that carbohydrates and amino acids were the main carbon sources of rhizosphere microorganisms.To conclude,the application of winter cover crop and straw returning to paddy fields could significantly increase the carbon source utilization, species richness,and species evenness of rhizosphere microflora in double-crop rice paddies.展开更多
Based on the analysis of the bioenergy crop production function of land use,combined with the status quo of Chinese land use,the cultivation of energy plants and the bioenergy crop production function of land use had ...Based on the analysis of the bioenergy crop production function of land use,combined with the status quo of Chinese land use,the cultivation of energy plants and the bioenergy crop production function of land use had been analyzes and discusses in this paper.Results show that there were a lot of unused lands and marginal lands which can be planted bioenergy crops to perform the bioenergy crop production function of land use with great potentials;and currently there were no food production problems.Therefore,it was very important for China to emphasize bioenergy crops planting in order to fully use land resources in our country,moderate the energy crisis and increase peasants' income.展开更多
Germplasm effect reflects the quantitative relation between production ability of germplasm elements and yield （quality） of a certain crop, which can be shown by mathematic function, namely, germplasm effect functio...Germplasm effect reflects the quantitative relation between production ability of germplasm elements and yield （quality） of a certain crop, which can be shown by mathematic function, namely, germplasm effect function. Germplasm effect of a crop variety is an aggregation of many effective factors, and is restrained by different effective factors; constant increase of any one effect of germplasm elements would lead to law of effect decline, therefore, possible modes of transgenic crops effect function were deduced according to the law of effect decline. The possible modes of single transgenic germplasm effect function and multi-transgenic germplasm effect regression equation were discussed, and the characteristics of germplasm effect regression equation were analyzed in this paper.展开更多
With the advances in genomics and bioinformatics,particularly the extensive application of high-throughput sequencing technology,a large number of non-coding RNAs(ncRNAs)have been discovered,of which long ncRNAs(lncRN...With the advances in genomics and bioinformatics,particularly the extensive application of high-throughput sequencing technology,a large number of non-coding RNAs(ncRNAs)have been discovered,of which long ncRNAs(lncRNAs)refer to a class of transcripts that are more than 200 nucleotides in length.Accumulating evidence demonstrates that lncRNAs play significant roles in a wide range of biological processes,including regulating plant growth and development as well as modulating biotic and abiotic stress responses.Although the study of lncRNAs has been a hotspot of biological research in recent years,the functional characteristics of plant lncRNAs are still in their initial phase and face great challenges.Here,we summarize the characteristics and screening methods of lncRNAs and highlight their biological functions in major vegetable crops,including tomato,Brassica genus crops,cucumber,pepper,carrot,radish,potato,and spinach,which are implicated in the interaction of lncRNAs and miRNAs.This review enhances the understanding of lncRNAs'roles and can guide crop improvement programs in the future.展开更多
[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous ...[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.展开更多
Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).Ho...Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).展开更多
Cr（Ⅲ） adsorption by biochars generated from peanut, soybean, canola and rice straws is investigated with batch methods. Adsorption of Cr（Ⅲ） increased as pH rose from 2.5 to 5.0. Adsorption of Cr（Ⅲ） led to pea...Cr（Ⅲ） adsorption by biochars generated from peanut, soybean, canola and rice straws is investigated with batch methods. Adsorption of Cr（Ⅲ） increased as pH rose from 2.5 to 5.0. Adsorption of Cr（Ⅲ） led to peak position shifts in the FFIR-PAS spectra of the biochars and made zeta potential values less negative, suggesting the formation of surface complexes between Cr^3＋ and functional groups on the biochars. The adsorption capacity of Cr（Ⅲ） followed the order： peanut straw char 〉 soybean straw char 〉 canola straw char 〉 rice straw char, which was consistent with the content of acidic functional groups on the biochars. The increase in Cr^3＋ hydrolysis as the pH rose was one of the main reasons for the increased adsorption of Cr（Ⅲ） by the biochars at higher pH values. Cr（llI） can be adsorbed by the biochars through electrostatic attraction between negative surfaces and Cr^3＋, but the relative contribution of electrostatic adsorption was less than 5%. Therefore, Cr（Ⅲ） was mainly adsorbed by the biochars through specific adsorption. The Langumir and Freundlich equations fitted the adsorption isotherms well and can therefore be used to describe the adsorption behavior of Cr（Ⅲ） by the crop straw biochars. The crop straw biochars have great adsorption capacities for Cr（Ⅲ） under acidic conditions and can be used as adsorbents to remove Cr（Ⅲ） from acidic wastewaters.展开更多
Plant growth regulators are biologically active signaling molecules that regulate a number of plant physiological processes. Auxin(indole-3-acetic acid) is an important plant growth regulator and is synthesized within...Plant growth regulators are biologically active signaling molecules that regulate a number of plant physiological processes. Auxin(indole-3-acetic acid) is an important plant growth regulator and is synthesized within plant tissues through L-tryptophan(L-TRP)-dependent and-independent pathways. It has been found that plants respond to exogenously applied L-TRP due to insufficient endogenous auxin biosynthesis. The exogenous application of L-TRP is highly significant for normal plant growth and development.L-tryptophan is applied through foliar spray, seed priming, and soil application. Soil-applied L-TRP is either directly taken up by plants or metabolized to auxin by soil microbiota and then absorbed by plant roots. Similarly, foliar spray and seed priming with L-TRP stimulates auxin synthesis within plants and improves the growth and productivity of agricultural crops. Furthermore, L-TRP contains approximately 14% nitrogen(N) in its composition, which is released upon its metabolism within a plant or in the rhizosphere and plays a role in enhancing crop productivity. This review deals with assessing crop responses under the exogenous application of L-TRP in normal and stressed environments, mode of action of L-TRP, advantages of using L-TRP over other auxin precursors, and role of the simultaneous use of L-TRP and auxin-producing microbes in improving the productivity of agricultural crops. To the best of our knowledge, this is the first review reporting the importance of the use of L-TRP in agriculture.展开更多
An increase in crop intensity could improve crop yield but may also lead to a series of environmental problems, such as depletion of ground water and increased soil salinity. The generation of high resolution(30 m) cr...An increase in crop intensity could improve crop yield but may also lead to a series of environmental problems, such as depletion of ground water and increased soil salinity. The generation of high resolution(30 m) crop intensity maps is an important method used to monitor these changes, but this is challenging because the temporal resolution of the 30-m image time series is low due to the long satellite revisit period and high cloud coverage. The recently launched Sentinel-2 satellite could provide optical images at 10–60 m resolution and thus improve the temporal resolution of the 30-m image time series. This study used harmonized Landsat Sentinel-2(HLS) data to identify crop intensity. The sixth polynomial function was used to fit the normalized difference vegetation index(NDVI) and enhanced vegetation index(EVI) curves. Then, 15-day NDVI and EVI time series were then generated from the fitted curves and used to generate the extent of croplands. Lastly, the first derivative of the fitted VI curves were used to calculate the VI peaks;spurious peaks were removed using artificially defined thresholds and crop intensity was generated by counting the number of remaining VI peaks. The proposed methods were tested in four study regions, with results showing that 15-day time series generated from the fitted curves could accurately identify cropland extent. Overall accuracy of cropland identification was higher than 95%. In addition, both the harmonized NDVI and EVI time series identified crop intensity accurately as the overall accuracies, producer’s accuracies and user’s accuracies of non-cropland, single crop cycle and double crop cycle were higher than 85%. NDVI outperformed EVI as identifying double crop cycle fields more accurately.展开更多
基金supported by grants from the Guangxi Science and Technology Major Project(GKAA24206023)the Biological Breeding-National Science and Technology Major Project(2024ZD04077)+2 种基金the National Natural Science Foundation of China(32272120)the National Key Research and Development Program of China(2024YFF1000800)the Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops Major Project(FCBRCE-202502,FCBRCE-202504).
文摘A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synthetic metabolic engineering offers a method to modify and redesign metabolic pathways to increase the nutritional value of crops.We summarize recent advances in the biofortification of key nutrients including provitamin A,vitamin C,vitamin B9,iron,zinc,anthocyanins,flavonoids,and unsaturated fatty acids.We discuss the potential of multi-gene stacking,gene editing,enzyme engineering,and artificial intelligence in synthetic metabolic engineering.We propose future research directions and potential solutions centered on leveraging AI-driven systems biology,precision gene editing,enzyme engineering,agrobacterium-mediated genotype-independent transformation,and modular metabolic engineering strategies to develop next-generation nutritionally enhanced super crops and transform global food systems.
文摘[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum.
文摘Increased human and industrial activities have exacerbated the release of toxic materials and acute envi-ronmental pollution in recent times.Biochar,a carbon-rich material produced from biomass,is gaining momentum as a versatile material for attaining a sustainable environment.The study reviews the application of functionalized biochar for energy storage,environmental remediation,catalysis,and sustainable agriculture,aiming to achieve a greener future.Thedeployment of crop residues as a renewable feedstock for biochar,and their properties,compositions,modification,and functionalization techniques are also discussed.Additionally,the avenues for applying functionalized biochar to achieve a greener future,future trends and innovations,challenges,and future research directions are highlighted.Despite the limitations of scalability,ecotoxicological risks,logistical issues,lack of characterization protocols,high production costs,poor social acceptance,and inadequate policy and regulatory frameworks,functionalized biochar offers a better surface area,improved porosity,enhanced functional groups,and higher recoverability,leading to improved performance,adsorption capacity,biodegradability,and applications in specialized fields.Future research should prioritize standardization,scalability,cost reduction strategies,expansion of application areas,integration of emerging tools such as artificial intelligence and predictive modeling,and the development of policy and regulatory frameworks,ensuring that biochar’s full potential is harnessed effectively to support a low-carbon,resource-efficient future and global sustainability goals.
基金Supported by Hebei Provincial Key R&D projects(21327306D)Hebei Provincial Key R&D projects(20326807D)Chengde Science and Technology Research and Development Planning Project(202103B003).
文摘Plant growth-promoting bacteria(PGPBs)can promote plant growth and improve crop yield.They can induce plant systemic resistance to resist biotic and abiotic stresses.In recent years,with the development of green ecological agriculture,new biological fertilizers such as microbial inocula and microbial fertilizers based on PGPBs have been gradually applied in crop planting.Based on plant growth promotion and disease control,the application progress of PGPBs in crops from the aspects of growth promotion mechanism,growth promotion effect,resistance to biological and abiotic stresses were discussed,aiming to provide reference for the relevant research and application of PGPBs in crops.
文摘The functional diversity of rhizosphere microflora which is also known as the “microbial community” is a sensitive indicator of soil quality subject to the type of winter cover crop and straw returning.In order to evaluate the effects of different winter cover crops and returning patterns on the functional diversity of rhizosphere microflora in double-crop rice paddies,we designed five winter cover crops and straw returning combinations to analyze their effects on the functional diversity of rhizosphere microflora in rice paddies: ryegrass (Lolium multiflorumL.)–double-crop rice (Ry),milk vetch (Astragalus sinicus L.)–double-crop rice (Mv),Rape (Brassica napus L.)–Double-crop rice (Ra), Potato (Solanum tuberosum L.)–double-crop rice (Po),and winter fallow–doublecrop rice (CK,the control).In this paper,the average well color development (AWCD) in Biolog-GN plates indicated the capacity for carbon utilization by the rhizosphere microbial community.We analyzed the rhizosphere microbial community functional diversity of the paddy soils with the above five treatments by using the Biolog-GN system.The results showed that applications of winter cover crop and straw returning caused high increases in AWCD compared with CK,and the AWCD values for samples with Po treatment was greater than those with Ry and CK treatments at the early and late rice maturity stages. It was concluded that applications of winter cover crop and straw returning can cause changes in the carbon utilization efficiency of rhizosphere microflora.There were differences in the genetic diversity of the rhizosphere microflora among different treatments at the maturity stage of early and late rice.The richness,Shannon,and McIntosh Index under different winter cover crop and straw returning treatments were significantly different.The highest indexes were observed in the Po treatment and the lowest in the CK at the maturity stage of early and late rice.The richness, Shannon,and McIntosh Index under different treatments ranked in descending order is as follows: Po>Ra>Mv>Ry>CK.Principal Component Analysis (PCA) of substrate reactions were conducted in this research.The results indicated that the pattern of carbon source utilization varied with winter cover crop treatments,and that carbohydrates and amino acids were the main carbon sources of rhizosphere microorganisms.To conclude,the application of winter cover crop and straw returning to paddy fields could significantly increase the carbon source utilization, species richness,and species evenness of rhizosphere microflora in double-crop rice paddies.
文摘Based on the analysis of the bioenergy crop production function of land use,combined with the status quo of Chinese land use,the cultivation of energy plants and the bioenergy crop production function of land use had been analyzes and discusses in this paper.Results show that there were a lot of unused lands and marginal lands which can be planted bioenergy crops to perform the bioenergy crop production function of land use with great potentials;and currently there were no food production problems.Therefore,it was very important for China to emphasize bioenergy crops planting in order to fully use land resources in our country,moderate the energy crisis and increase peasants' income.
文摘Germplasm effect reflects the quantitative relation between production ability of germplasm elements and yield （quality） of a certain crop, which can be shown by mathematic function, namely, germplasm effect function. Germplasm effect of a crop variety is an aggregation of many effective factors, and is restrained by different effective factors; constant increase of any one effect of germplasm elements would lead to law of effect decline, therefore, possible modes of transgenic crops effect function were deduced according to the law of effect decline. The possible modes of single transgenic germplasm effect function and multi-transgenic germplasm effect regression equation were discussed, and the characteristics of germplasm effect regression equation were analyzed in this paper.
基金supported by the National Natural Science Foundation of China (32172583)Natural Science Foundation of Hebei (C2021209005,C2021209019)the China Postdoctoral Science Foundation (2020M673188,2021T140097).
文摘With the advances in genomics and bioinformatics,particularly the extensive application of high-throughput sequencing technology,a large number of non-coding RNAs(ncRNAs)have been discovered,of which long ncRNAs(lncRNAs)refer to a class of transcripts that are more than 200 nucleotides in length.Accumulating evidence demonstrates that lncRNAs play significant roles in a wide range of biological processes,including regulating plant growth and development as well as modulating biotic and abiotic stress responses.Although the study of lncRNAs has been a hotspot of biological research in recent years,the functional characteristics of plant lncRNAs are still in their initial phase and face great challenges.Here,we summarize the characteristics and screening methods of lncRNAs and highlight their biological functions in major vegetable crops,including tomato,Brassica genus crops,cucumber,pepper,carrot,radish,potato,and spinach,which are implicated in the interaction of lncRNAs and miRNAs.This review enhances the understanding of lncRNAs'roles and can guide crop improvement programs in the future.
基金Supported by Agricultural Science and Technology Innovation Fund of Hunan Province(XCNZ[2021]No.15)Loudi Science and Technology Innovation Program(LKF[2022]29)+1 种基金Applied Characteristic Discipline Construction Project of Hunan Province:Plant ProtectionPostgraduate Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2022Y12).
文摘[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.
基金supported by the Jilin Science and Technology Development Program,China (20240602032RC)the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZD001)+1 种基金the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZY012)the Jilin Province Development and Reform Commission-Project for Improving the Independent Innovation Capacity of Major Grain Crops,China (2024C002)。
文摘Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).
基金supported by the National Natural Science Foundation of China(No.41230855)the National Key Technology R&D Program of China(No.2012BAJ24B06)
文摘Cr（Ⅲ） adsorption by biochars generated from peanut, soybean, canola and rice straws is investigated with batch methods. Adsorption of Cr（Ⅲ） increased as pH rose from 2.5 to 5.0. Adsorption of Cr（Ⅲ） led to peak position shifts in the FFIR-PAS spectra of the biochars and made zeta potential values less negative, suggesting the formation of surface complexes between Cr^3＋ and functional groups on the biochars. The adsorption capacity of Cr（Ⅲ） followed the order： peanut straw char 〉 soybean straw char 〉 canola straw char 〉 rice straw char, which was consistent with the content of acidic functional groups on the biochars. The increase in Cr^3＋ hydrolysis as the pH rose was one of the main reasons for the increased adsorption of Cr（Ⅲ） by the biochars at higher pH values. Cr（llI） can be adsorbed by the biochars through electrostatic attraction between negative surfaces and Cr^3＋, but the relative contribution of electrostatic adsorption was less than 5%. Therefore, Cr（Ⅲ） was mainly adsorbed by the biochars through specific adsorption. The Langumir and Freundlich equations fitted the adsorption isotherms well and can therefore be used to describe the adsorption behavior of Cr（Ⅲ） by the crop straw biochars. The crop straw biochars have great adsorption capacities for Cr（Ⅲ） under acidic conditions and can be used as adsorbents to remove Cr（Ⅲ） from acidic wastewaters.
文摘Plant growth regulators are biologically active signaling molecules that regulate a number of plant physiological processes. Auxin(indole-3-acetic acid) is an important plant growth regulator and is synthesized within plant tissues through L-tryptophan(L-TRP)-dependent and-independent pathways. It has been found that plants respond to exogenously applied L-TRP due to insufficient endogenous auxin biosynthesis. The exogenous application of L-TRP is highly significant for normal plant growth and development.L-tryptophan is applied through foliar spray, seed priming, and soil application. Soil-applied L-TRP is either directly taken up by plants or metabolized to auxin by soil microbiota and then absorbed by plant roots. Similarly, foliar spray and seed priming with L-TRP stimulates auxin synthesis within plants and improves the growth and productivity of agricultural crops. Furthermore, L-TRP contains approximately 14% nitrogen(N) in its composition, which is released upon its metabolism within a plant or in the rhizosphere and plays a role in enhancing crop productivity. This review deals with assessing crop responses under the exogenous application of L-TRP in normal and stressed environments, mode of action of L-TRP, advantages of using L-TRP over other auxin precursors, and role of the simultaneous use of L-TRP and auxin-producing microbes in improving the productivity of agricultural crops. To the best of our knowledge, this is the first review reporting the importance of the use of L-TRP in agriculture.
基金supported by the China Postdoctoral Science Foundation (2017M620075 and BX201700286)the National Natural Science Foundation of China (NSFC-61661136006)
文摘An increase in crop intensity could improve crop yield but may also lead to a series of environmental problems, such as depletion of ground water and increased soil salinity. The generation of high resolution(30 m) crop intensity maps is an important method used to monitor these changes, but this is challenging because the temporal resolution of the 30-m image time series is low due to the long satellite revisit period and high cloud coverage. The recently launched Sentinel-2 satellite could provide optical images at 10–60 m resolution and thus improve the temporal resolution of the 30-m image time series. This study used harmonized Landsat Sentinel-2(HLS) data to identify crop intensity. The sixth polynomial function was used to fit the normalized difference vegetation index(NDVI) and enhanced vegetation index(EVI) curves. Then, 15-day NDVI and EVI time series were then generated from the fitted curves and used to generate the extent of croplands. Lastly, the first derivative of the fitted VI curves were used to calculate the VI peaks;spurious peaks were removed using artificially defined thresholds and crop intensity was generated by counting the number of remaining VI peaks. The proposed methods were tested in four study regions, with results showing that 15-day time series generated from the fitted curves could accurately identify cropland extent. Overall accuracy of cropland identification was higher than 95%. In addition, both the harmonized NDVI and EVI time series identified crop intensity accurately as the overall accuracies, producer’s accuracies and user’s accuracies of non-cropland, single crop cycle and double crop cycle were higher than 85%. NDVI outperformed EVI as identifying double crop cycle fields more accurately.
