The mammalian liver has a very strong regeneration capacity after partial hepa- tectomy (PH). To further learn the genes participating in the liver regeneration (LR), 551 cDNAs selected from subtract...The mammalian liver has a very strong regeneration capacity after partial hepa- tectomy (PH). To further learn the genes participating in the liver regeneration (LR), 551 cDNAs selected from subtracted cDNA libraries of the regenerating rat liver were screened by microarray, and their expression pro?les were studied by cluster and generalization analyses. Among them, 177 genes were identi?ed unre- ported and up- or down-regulated more than twofold at one or more time points after PH, of which 62 genes were down-regulated to less than 0.5; 99 genes were up-regulated to 2–10 folds, and 16 genes were either up- or down-regulated at dif- ferent time points during LR. By using BLAST and GENSCAN, these genes were located on responsible chromosomes with 131 genes on the long arms of the chro- mosomes. The cluster and generalization analyses showed that the gene expression pro?les are similar in 2 and 4, 12 and 16, 96 and 144 h respectively after PH, suggesting that the actions of the genes expressed in the same pro?les are similar, and those expressed in di?erent pro?les have less similarity. However, the types, characteristics and functions of the 177 genes remain to be further studied.展开更多
基金This work was supported by National Natural Science Foundation of China (No. 30270673).
文摘The mammalian liver has a very strong regeneration capacity after partial hepa- tectomy (PH). To further learn the genes participating in the liver regeneration (LR), 551 cDNAs selected from subtracted cDNA libraries of the regenerating rat liver were screened by microarray, and their expression pro?les were studied by cluster and generalization analyses. Among them, 177 genes were identi?ed unre- ported and up- or down-regulated more than twofold at one or more time points after PH, of which 62 genes were down-regulated to less than 0.5; 99 genes were up-regulated to 2–10 folds, and 16 genes were either up- or down-regulated at dif- ferent time points during LR. By using BLAST and GENSCAN, these genes were located on responsible chromosomes with 131 genes on the long arms of the chro- mosomes. The cluster and generalization analyses showed that the gene expression pro?les are similar in 2 and 4, 12 and 16, 96 and 144 h respectively after PH, suggesting that the actions of the genes expressed in the same pro?les are similar, and those expressed in di?erent pro?les have less similarity. However, the types, characteristics and functions of the 177 genes remain to be further studied.
