Introduction:Type F Clostridium perfringens(C.perfringens)represents a significant pathogen in human gastrointestinal diseases,primarily through its cpe gene encoding C.perfringens enterotoxin(CPE).This investigation ...Introduction:Type F Clostridium perfringens(C.perfringens)represents a significant pathogen in human gastrointestinal diseases,primarily through its cpe gene encoding C.perfringens enterotoxin(CPE).This investigation examined the prevalence,antimicrobial resistance patterns,and genetic characteristics of Type F C.perfringens within the Chinese population.Methods:The study analyzed 2,068 stool samples collected from 11 provincial hospitals in 2024.Antimicrobial susceptibility testing was conducted following Clinical&Laboratory Standards Institute(CLSI)guidelines,while whole-genome sequencing provided detailed genetic profiles.Evolutionary relationships and clonal transmission patterns were investigated through phylogenetic and genetic environment analyses.Results:The prevalence of Type F C.perfringens was 2.38%,with isolates predominantly identified in human clinical samples and higher detection rates in gastroenterology departments.Notably,47.1%of isolates demonstrated high resistance to metronidazole,while all exhibited intermediate resistance to erythromycin.Phylogenetic analysis revealed high similarity among isolates from patients within the same province(single-nucleotide polymorphism(SNPs)<100),and genetic environment analysis indicated potential horizontal gene transfer between animal and human strains.Conclusions:This investigation predominantly identified Type F C.perfringens in human clinical cases,with sporadic detection in pets and food products.These findings highlight the emergence of Type F C.perfringens outbreaks among diarrheal patients,emphasizing the necessity for targeted interventions as virulence factors increase.展开更多
基金Supported by the National Key Research and Development Program of China(No.2022YFD1800400)the National Natural Science Foundation of China(No.22193064).
文摘Introduction:Type F Clostridium perfringens(C.perfringens)represents a significant pathogen in human gastrointestinal diseases,primarily through its cpe gene encoding C.perfringens enterotoxin(CPE).This investigation examined the prevalence,antimicrobial resistance patterns,and genetic characteristics of Type F C.perfringens within the Chinese population.Methods:The study analyzed 2,068 stool samples collected from 11 provincial hospitals in 2024.Antimicrobial susceptibility testing was conducted following Clinical&Laboratory Standards Institute(CLSI)guidelines,while whole-genome sequencing provided detailed genetic profiles.Evolutionary relationships and clonal transmission patterns were investigated through phylogenetic and genetic environment analyses.Results:The prevalence of Type F C.perfringens was 2.38%,with isolates predominantly identified in human clinical samples and higher detection rates in gastroenterology departments.Notably,47.1%of isolates demonstrated high resistance to metronidazole,while all exhibited intermediate resistance to erythromycin.Phylogenetic analysis revealed high similarity among isolates from patients within the same province(single-nucleotide polymorphism(SNPs)<100),and genetic environment analysis indicated potential horizontal gene transfer between animal and human strains.Conclusions:This investigation predominantly identified Type F C.perfringens in human clinical cases,with sporadic detection in pets and food products.These findings highlight the emergence of Type F C.perfringens outbreaks among diarrheal patients,emphasizing the necessity for targeted interventions as virulence factors increase.
