Background Fatty liver syndrome is a prevalent metabolic disorder in transition dairy cows,characterized by excessive hepatic lipid accumulation that impairs liver function and leads to systemic metabolic disturbances...Background Fatty liver syndrome is a prevalent metabolic disorder in transition dairy cows,characterized by excessive hepatic lipid accumulation that impairs liver function and leads to systemic metabolic disturbances.Docosahexaenoic acid(DHA),a prominent n-3 polyunsaturated fatty acid(PUFA),not only exhibits anti-inflammatory and anti-oxidative properties,but also holds potential in ameliorating lipid metabolism.This study integrated in vitro bovine primary hepatocyte models and in vivo dairy cow trials to investigate the regulatory effects of DHA on hepatic lipid deposition.Results In vitro,40μmol/L DHA significantly reduced triglyceride(TAG)accumulation in steatotic hepatocytes by downregulating genes involved in fatty acid transport(FABP-1,CD36)and lipogenesis(DGAT2,FAS,SREBP-1C),while upregulating markers of lipolysis(CGI-58,ATGL)and fatty acid oxidation(ACADL,CPT1A,CPT2).Transmission electron microscopy(TEM)confirmed DHA-mediated restoration of mitochondrial ultrastructure and enhanced lipid droplet(LD)-mitochondria interactions.In vivo,dietary rumen-protected DHA(180 g/d)supplementation reduced hepatic lipid deposition,improved liver function(evidenced by decreased total bilirubin and alanine aminotransferase),reduced oxidative stress and inflammation(suppressed malondialdehyde,glutathione peroxidase,and lipopolysaccharide),coincided with relieving insulin resistance(reduced insulin and glucose,as well increased adiponectin)in dairy cows with fatty liver.These improvements may be attributed to increased expression of TOMM20 and MtCo-1,promoting mitochondrial biogenesis andβ-oxidation,along with an elevated plasma n-3/n-6 ratio.Conclusions Collectively,these findings suggest that DHA supplementation represents a promising nutritional strategy for preventing spontaneous fatty liver in transition dairy cows by enhancing hepatic lipid clearance and restoring metabolic homeostasis.展开更多
Background During the transition period,cows are prone to negative energy balance,which can lead to a decline in production performance and health in severe cases.In recent years,it has been discovered that bile acids...Background During the transition period,cows are prone to negative energy balance,which can lead to a decline in production performance and health in severe cases.In recent years,it has been discovered that bile acids(BAs)can act not only as fat emulsifiers but also as signaling molecules to regulate body metabolism.Although BAs have been used to some extent in monogastric and aquatic animals,their role in ruminants,particularly in transition cows,remains unclear.Therefore,this study aimed to determine the effects of BAs on the production performance,milk and plasma fatty acid and BA composition,and fecal microbiota in transition dairy cows.Results Forty-six healthy transition Holstein dairy cows with similar conditions were randomly divided into two groups and supplemented with 0 or 20 g/d of BAs from 21 d before the expected calving to 21 d after calving.The production performance was tracked until 60 d after calving.The results indicated that BA supplementation significantly improved postpartum milk fat content and yields as well as the yields of unsaturated fatty acids,monounsaturated fatty acids,and polyunsaturated fatty acids in milk.There was a significant increase in the concentration of triglyceride and the proportion of C≤16 fatty acids in the plasma of cows supplemented with BAs,while the concentration of β-hydroxybutyrate and the proportion of C>16 fatty acids in the plasma decreased significantly.BA supplementation significantly altered the composition of the fecal bacterial community and increased the relative abundance of bacteria beneficial for BA metabolism and transformation(Romboutsia,Clostridium sensu_stricto_6,and Clostridium sensu_stricto_1).Functional prediction analysis showed that the relative abundance of bile salt hydrolase,7 α-hydroxysteroid dehydrogenase,and BA inducible E as well as the pathways related to BA metabolism also significantly increased in cows supplemented BAs.In addition,BA supplementation significantly altered the composition of plasma and fecal BAs,particularly increasing circulating secondary BA concentration,which might induce the complete oxidation of fatty acids in the liver and further reduce the concentration of β-hydroxybutyrate.Conclusions These findings highlight the potential benefits of BA supplementation in improving milk yields and quality,as well as influencing metabolic pathways in transition dairy cows.Meanwhile,further studies are needed to elucidate the underlying mechanisms and explore the broader implications of these results by using more tissue samples.展开更多
Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the envi...Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the environment as ammonia gas leading to environmental acidification and nutrient enrichment of sensitive habitats,and to watercourses contributing to aquatic eutrophication.While there is much evidence that NUE can be improved by reducing the crude protein(CP)content of dairy cow diets,the long-term impacts of feeding lower protein diets on cow performance and the rumen microbiome are less well understood.This study examined the effects of reducing the CP contents of dairy cow diets on cow performance,NUE,the relationship between NUE and residual feed intake(RFI),and the rumen microbiome.Results Dietary CP content did not affect feed intake,milk yield or milk composition(P>0.05),except for milk urea N(MUN),which increased with increasing diet CP content(P<0.05).The mean NUE was 34%,34%and 31%for the LCP(low-protein,15%),MCP(medium-protein,16%),and HCP(high-protein,17%)diets,respectively.RFI was negatively correlated with NUE(r=−0.57,P<0.001).The rumen ammonia-N concentrations increased with increasing dietary CP;however,the ruminal pH and volatile fatty acid(VFA)content of the rumen fluid remained constant.Predicted urinary N excretion was greater in the HCP and MCP diets than in the LCP diet.Reducing dietary CP content in dairy cow diets did not affect microbial composition,diversity and functional profiles.The family Bacteroidaceae was more abundant in HE(high-efficiency)cows,whereas the Methanobacteriaceae and the genus Methanobrevibacter were more abundant in LE(low-efficiency)cows.Additionally,propanoate metabolism,cysteine and methionine metabolism and amino acid biosynthesis pathways were more abundant in HE cows,whilst the methane(CH4)metabolism pathway was upregulated in LE cows.Conclusions The results demonstrate that diet CP can be reduced with no loss in cow performance,but with an associated reduction in N excretion.The abundance of microbial populations differed between low and high efficiency cows,which may contribute to the differences in efficiency observed.展开更多
This study focuses on the design and validation of a behavior classification system for cattle using behavioral data collected through accelerometer sensors.Data collection and behavioral analysis are achieved using m...This study focuses on the design and validation of a behavior classification system for cattle using behavioral data collected through accelerometer sensors.Data collection and behavioral analysis are achieved using machine learning(ML)algorithms through accelerometer sensors.However,behavioral analysis poses challenges due to the complexity of cow activities.The task becomes more challenging in a real-time behavioral analysis system with the requirement for shorter data windows and energy constraints.Shorter windows may lack sufficient information,reducing algorithm performance.Additionally,the sensor’s position on the cowsmay shift during practical use,altering the collected accelerometer data.This study addresses these challenges by employing a 3-s data window to analyze cow behaviors,specifically Feeding,Lying,Standing,and Walking.Data synchronization between accelerometer sensors placed on the neck and leg compensates for the lack of information in short data windows.Features such as the Vector of Dynamic Body Acceleration(VeDBA),Mean,Variance,and Kurtosis are utilized alongside the Decision Tree(DT)algorithm to address energy efficiency and ensure computational effectiveness.This study also evaluates the impact of sensor misalignment on behavior classification.Simulated datasets with varying levels of sensor misalignment were created,and the system’s classification accuracy exceeded 0.95 for the four behaviors across all datasets(including original and simulated misalignment datasets).Sensitivity(Sen)and PPV for all datasets were above 0.9.The study provides farmers and the dairy industry with a practical,energy-efficient system for continuously monitoring cattle behavior to enhance herd productivity while reducing labor costs.展开更多
Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G(IgG)content.Immediately after calving,colostrum samples were collected from 18 cows...Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G(IgG)content.Immediately after calving,colostrum samples were collected from 18 cows to measure IgG concentration.Based on colostrum IgG content,samples were classified through cluster analysis and were identified as poor,average,and excellent quality.The proteome was assessed with quantitative shotgun proteomics;abundance data were compared among the colostrum types;enrichment analysis of metabolic processes and proteins classes was performed as well.We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.Results On average,428 proteins were identified per sample,which belonged mainly to cellular process,biological regulation,response to stimulus,metabolic process,and immune system process.Most abundant proteins were complement C3(Q2UVX4),alpha-S1-casein(P02662),Ig-like domain-containing protein(A0A3Q1M032),albumin(A0A140T897),polymeric immunoglobulin receptor(P81265),lactotransferrrin(P24627),and IGHG1*01(X167014).Colostrum of excellent quality had greater(P<0.05)abundance of serpin A3-7(A2I7N3),complement factorl(A0A3Q1 MIF4),lipocalin/cytosolic fatty-acid binding domain-containing protein(A0A3Q1 MRQ2),complement C3(E1B805),complement component 4 binding protein alpha(A0AAF6ZHP5),and complement component C6(F1MM86).However,colostrum of excellent quality had lower(P<0.05)abundance of HGF activator(E1BCW0),alpha-S1-casein(P02662),and xanthine dehydrogenase/oxidase(P80457).This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway,complement activation,complement activation classical pathway,humoral immune response,leukocyte mediated immunity,and negative regulation of endopeptidase activity in excellent-quality colostrum.Additionally,some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of calves(P<0.05;r≥0.57).Conclusions This study provides new insights into the bovine colostrum proteome,demonstrating associations between IgG levels and the abundance of other proteins,as well as the enrichment of metabolic processes related to innate immune response.Thus,results suggest that the colostrum proteomic profile is associated with the content of IgG.Future research should deeply explore the association of these findings with pre-calving nutrition status and blood composition of the cow,and with passive immunity transfer to the calf.展开更多
A lactating dairy cow mammary epithelial cell(DCMEC)model is useful for studying milk synthesis and lactation in the dairy cow mammary gland.However,the cells can only maintain their original characteristics for a lim...A lactating dairy cow mammary epithelial cell(DCMEC)model is useful for studying milk synthesis and lactation in the dairy cow mammary gland.However,the cells can only maintain their original characteristics for a limited period in vitro.Thus,the proliferative capacity and lactation pattern of subcultured DCMECs need to be characterized.In the present study,subcultured DCMECs appeared to proliferate without changes in morphology or growth pattern up to the 12th passage.Subculturing had no obvious effect on the lactation capacity of the subcultured DCMEC up to the 10th passage in vitro.These results indicated that the DCMEC line could be subpassaged up to 10 times and still maintain its original milk synthesis characteristics.Moreover,DCMECs were frozen using different methods,and the method of placing the vials in the foam box was determined after cryopreservation with the best adherence of 91.2%.This method was recommended for long-term cryopreservation for DCMECs.展开更多
To address the issue of low recognition accuracy for eight types of behaviors including standing,walking,drinking,lying,eating,mounting,fighting and limping in complex multi-cow farm environments,a multi-target cow be...To address the issue of low recognition accuracy for eight types of behaviors including standing,walking,drinking,lying,eating,mounting,fighting and limping in complex multi-cow farm environments,a multi-target cow behavior recognition method based on an improved YOLOv11n algorithm was proposed.The detection capability for small targets in images was enhanced by incorporating a DASI module into the backbone network and a MDCR module into the neck network,based on YOLOv11.The improved YOLOv11 algorithm increased the mean average precision from the original 89.5%to 93%,with particularly notable improvements of 8.7%and 6.3%in the average precision for recognizing drinking and walking behaviors,respectively.These results fully demonstrate that the proposed method enhances the model s ability to recognize cow behaviors.展开更多
Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced...Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced enterocolitis syndrome,and eosinophilic esophagitis.This diagnostic overlap makes distin-guishing CMPA from its mimics difficult,resulting in potential misdiagnoses and unnecessary dietary restrictions.This review aims to comprehensively analyze CMPA and its mimicking conditions,highlighting their clinical presentations,diagnostic approaches,and management strategies to enhance diagnostic accu-racy and optimize patient care.A systematic literature search was conducted using PubMed,Scopus,Web of Science,and Google Scholar,focusing on studies published within the last 20 years.Articles addressing CMPA and its mimicking conditions were selected,with data synthesized into comparative analyses of diagnostic methods and management strategies.Accurate differentiation between CMPA and its mimics requires a thorough clinical evaluation supported by diagnostic tests such as skin prick tests,serum-specific IgE,and oral food challenges.Misdiagnosis can lead to nutritional deficiencies,psychological stress,and increased healthcare costs.Emerging diagnostic technologies,including component-resolved diagnostics and cytokine profiling,offer promising avenues for improving accuracy.A multidisciplinary approach involving pediatricians,allergists,and dietitians is essential for precise diagnosis and effective management.Ongoing research and education are crucial to enhancing clinical outcomes and reducing the burden on families.展开更多
[Objective] The research aimed to explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein cow.[Method] 36 Holstein cows with similar birth order and milk yield were equally ...[Objective] The research aimed to explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein cow.[Method] 36 Holstein cows with similar birth order and milk yield were equally and randomly divided into 3 groups which were control group, Chinese medicine Ⅰ group and Chinese medicine Ⅱ group. In control group, cows were fed with basic feed, while in Chinese medicine Ⅰ group, cows were fed with basic feed containing 4% compound Chinese herb additive and in Chinese medicine Ⅱ group, cows were fed with basic feed containing 3% compound Chinese herb additive.All cows in the 3 groups were analyzed comparatively.[Result] Adding some compound Chinese herb additive into basic feed would increase milk yield and anti-heat stress ability of Holstein cow,besides,it also could decrease cow diseases such as cow mastitis.Chinese medicine Ⅰ would generate the highest economic benefit of practical production.[Conclusion] Adding Chinese herb additive into basic feed is an effective way for reliefing heat stress and increasing milk yield.展开更多
[Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperatur...[Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperature,moisture,pH value,crude fiber,TOC,TN,GI during composting.[Result] The results showed that adding complex enzymes could accelerate degradation of organic matter in pre-composting period.Crude fiber of 2.0%,.1.5% enzyme treatment group and control group decreased 49.6%,47% and 29.1% respectively,TOC decreased 41.7%,35.3% and 21.1%,TN decreased 32.6%,26.8% and 19.2%.2.0%,1.5% enzyme treatment groups could reach basic maturity degree at 30 d.[Conclusion] Composting cycle be shortened by adding complex enzymes,which was useful for maturity of cow dung compost.展开更多
[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided...[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided into four groups, and were fed with forage supplemented with 0 （control group）, 0.2%, 0.4% and 0.6% Zengrujianniusan for 60 d. During this period, the contents of fat, protein and non-fat solid in milk were measured every 20 d. [Result] The milk production of the cows whose forage was added with 0.2 %, 0.4 % and 0.6% Zengrujianniusan was 4.02%, 12.50%, 14.00% higher than that of the control （P〉0.05）. The herbal feed additive had no significant influence on the contents of fat, milk and non-fat solid in milk, but significantly reduced the number of somatic cells. [Conclusion] The study will provide reference for developing feed additives which is safe and non-toxic to cows and their milk.展开更多
Using a reversed-phase microemulsion polymerization method, polyoxometalates(POMs) CoW 11Ti loaded starch nanoparticles were prepared and structurally characterized by elemental analyses, IR, UV-Vis and ESR spectrosco...Using a reversed-phase microemulsion polymerization method, polyoxometalates(POMs) CoW 11Ti loaded starch nanoparticles were prepared and structurally characterized by elemental analyses, IR, UV-Vis and ESR spectroscopy. The particle size of CoW 11Ti/starch was estimated by transmission electron microscope(TEM) and the size ranges by a 1000HSA MALVIRN Zetasizer instrument. The result shows that the polyoxometalate retained the parent structure after encapsulated by starch microspheres, which are able to enhance the stability and antitumoral activity of POMs and decrease the toxicity of POMs as well.展开更多
为了探究过瘤胃蛋氨酸(Rumen-protected methionine,RPM)对泌乳高峰期奶牛生产性能和全肠道消化率的影响,试验选取16头处于泌乳高峰期,体重、生理状况相似的健康荷斯坦奶牛,随机分为2组。对照组每日饲喂基础TMR日粮,RPM组每头牛在基础...为了探究过瘤胃蛋氨酸(Rumen-protected methionine,RPM)对泌乳高峰期奶牛生产性能和全肠道消化率的影响,试验选取16头处于泌乳高峰期,体重、生理状况相似的健康荷斯坦奶牛,随机分为2组。对照组每日饲喂基础TMR日粮,RPM组每头牛在基础日粮的基础上,每日单独补饲15 g RPM。结果表明:(1)泌乳高峰期奶牛日粮中添加RPM不影响体重、体况评分和干物质采食量(DMI)(P>0.05);(2)添加RPM对奶牛的产奶量和乳成分影响不显著(P>0.05);(3)添加RPM有降低瘤胃丁酸含量的趋势(P=0.069);(4)添加RPM不影响奶牛的表观消化率(P>0.05);(5)RPM显著提高了奶牛血浆尿素氮和二氧化碳水平(P<0.05);(6)RPM对血液中蛋氨酸的含量没有影响,脯氨酸浓度显著降低(P<0.05),具有降低血浆精氨酸和天冬氨酸含量的趋势(0.05<P<0.10)。总体而言,泌乳高峰期奶牛补饲RPM可能通过提高蛋氨酸吸收率、氨基酸代谢和机体蛋白合成改善泌乳高峰期奶牛健康状况。展开更多
基金supported by the National Key R&D Program of China(No.2022YFD1301001)。
文摘Background Fatty liver syndrome is a prevalent metabolic disorder in transition dairy cows,characterized by excessive hepatic lipid accumulation that impairs liver function and leads to systemic metabolic disturbances.Docosahexaenoic acid(DHA),a prominent n-3 polyunsaturated fatty acid(PUFA),not only exhibits anti-inflammatory and anti-oxidative properties,but also holds potential in ameliorating lipid metabolism.This study integrated in vitro bovine primary hepatocyte models and in vivo dairy cow trials to investigate the regulatory effects of DHA on hepatic lipid deposition.Results In vitro,40μmol/L DHA significantly reduced triglyceride(TAG)accumulation in steatotic hepatocytes by downregulating genes involved in fatty acid transport(FABP-1,CD36)and lipogenesis(DGAT2,FAS,SREBP-1C),while upregulating markers of lipolysis(CGI-58,ATGL)and fatty acid oxidation(ACADL,CPT1A,CPT2).Transmission electron microscopy(TEM)confirmed DHA-mediated restoration of mitochondrial ultrastructure and enhanced lipid droplet(LD)-mitochondria interactions.In vivo,dietary rumen-protected DHA(180 g/d)supplementation reduced hepatic lipid deposition,improved liver function(evidenced by decreased total bilirubin and alanine aminotransferase),reduced oxidative stress and inflammation(suppressed malondialdehyde,glutathione peroxidase,and lipopolysaccharide),coincided with relieving insulin resistance(reduced insulin and glucose,as well increased adiponectin)in dairy cows with fatty liver.These improvements may be attributed to increased expression of TOMM20 and MtCo-1,promoting mitochondrial biogenesis andβ-oxidation,along with an elevated plasma n-3/n-6 ratio.Conclusions Collectively,these findings suggest that DHA supplementation represents a promising nutritional strategy for preventing spontaneous fatty liver in transition dairy cows by enhancing hepatic lipid clearance and restoring metabolic homeostasis.
基金supported by the National Center of Technology Innovation for Dairy(No.2024-JSGG-021)the National Natural Science Foundation of China(No.32102570)the Key Research and Development Project of Ningxia(No.2024BBF01006).
文摘Background During the transition period,cows are prone to negative energy balance,which can lead to a decline in production performance and health in severe cases.In recent years,it has been discovered that bile acids(BAs)can act not only as fat emulsifiers but also as signaling molecules to regulate body metabolism.Although BAs have been used to some extent in monogastric and aquatic animals,their role in ruminants,particularly in transition cows,remains unclear.Therefore,this study aimed to determine the effects of BAs on the production performance,milk and plasma fatty acid and BA composition,and fecal microbiota in transition dairy cows.Results Forty-six healthy transition Holstein dairy cows with similar conditions were randomly divided into two groups and supplemented with 0 or 20 g/d of BAs from 21 d before the expected calving to 21 d after calving.The production performance was tracked until 60 d after calving.The results indicated that BA supplementation significantly improved postpartum milk fat content and yields as well as the yields of unsaturated fatty acids,monounsaturated fatty acids,and polyunsaturated fatty acids in milk.There was a significant increase in the concentration of triglyceride and the proportion of C≤16 fatty acids in the plasma of cows supplemented with BAs,while the concentration of β-hydroxybutyrate and the proportion of C>16 fatty acids in the plasma decreased significantly.BA supplementation significantly altered the composition of the fecal bacterial community and increased the relative abundance of bacteria beneficial for BA metabolism and transformation(Romboutsia,Clostridium sensu_stricto_6,and Clostridium sensu_stricto_1).Functional prediction analysis showed that the relative abundance of bile salt hydrolase,7 α-hydroxysteroid dehydrogenase,and BA inducible E as well as the pathways related to BA metabolism also significantly increased in cows supplemented BAs.In addition,BA supplementation significantly altered the composition of plasma and fecal BAs,particularly increasing circulating secondary BA concentration,which might induce the complete oxidation of fatty acids in the liver and further reduce the concentration of β-hydroxybutyrate.Conclusions These findings highlight the potential benefits of BA supplementation in improving milk yields and quality,as well as influencing metabolic pathways in transition dairy cows.Meanwhile,further studies are needed to elucidate the underlying mechanisms and explore the broader implications of these results by using more tissue samples.
基金funded by UK Research and Innovation(UKRI)doctoral training grant no:BB/T008776/1the Department of Agriculture,Environment and Rural Affairs(DAERA)by Trouw Nutrition and by John Thompsons and Sons Ltd.
文摘Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the environment as ammonia gas leading to environmental acidification and nutrient enrichment of sensitive habitats,and to watercourses contributing to aquatic eutrophication.While there is much evidence that NUE can be improved by reducing the crude protein(CP)content of dairy cow diets,the long-term impacts of feeding lower protein diets on cow performance and the rumen microbiome are less well understood.This study examined the effects of reducing the CP contents of dairy cow diets on cow performance,NUE,the relationship between NUE and residual feed intake(RFI),and the rumen microbiome.Results Dietary CP content did not affect feed intake,milk yield or milk composition(P>0.05),except for milk urea N(MUN),which increased with increasing diet CP content(P<0.05).The mean NUE was 34%,34%and 31%for the LCP(low-protein,15%),MCP(medium-protein,16%),and HCP(high-protein,17%)diets,respectively.RFI was negatively correlated with NUE(r=−0.57,P<0.001).The rumen ammonia-N concentrations increased with increasing dietary CP;however,the ruminal pH and volatile fatty acid(VFA)content of the rumen fluid remained constant.Predicted urinary N excretion was greater in the HCP and MCP diets than in the LCP diet.Reducing dietary CP content in dairy cow diets did not affect microbial composition,diversity and functional profiles.The family Bacteroidaceae was more abundant in HE(high-efficiency)cows,whereas the Methanobacteriaceae and the genus Methanobrevibacter were more abundant in LE(low-efficiency)cows.Additionally,propanoate metabolism,cysteine and methionine metabolism and amino acid biosynthesis pathways were more abundant in HE cows,whilst the methane(CH4)metabolism pathway was upregulated in LE cows.Conclusions The results demonstrate that diet CP can be reduced with no loss in cow performance,but with an associated reduction in N excretion.The abundance of microbial populations differed between low and high efficiency cows,which may contribute to the differences in efficiency observed.
基金funded by Vietnam National Foundation for Science and Technology Development(NAFOSTED)under grant number:02/2022/TN.
文摘This study focuses on the design and validation of a behavior classification system for cattle using behavioral data collected through accelerometer sensors.Data collection and behavioral analysis are achieved using machine learning(ML)algorithms through accelerometer sensors.However,behavioral analysis poses challenges due to the complexity of cow activities.The task becomes more challenging in a real-time behavioral analysis system with the requirement for shorter data windows and energy constraints.Shorter windows may lack sufficient information,reducing algorithm performance.Additionally,the sensor’s position on the cowsmay shift during practical use,altering the collected accelerometer data.This study addresses these challenges by employing a 3-s data window to analyze cow behaviors,specifically Feeding,Lying,Standing,and Walking.Data synchronization between accelerometer sensors placed on the neck and leg compensates for the lack of information in short data windows.Features such as the Vector of Dynamic Body Acceleration(VeDBA),Mean,Variance,and Kurtosis are utilized alongside the Decision Tree(DT)algorithm to address energy efficiency and ensure computational effectiveness.This study also evaluates the impact of sensor misalignment on behavior classification.Simulated datasets with varying levels of sensor misalignment were created,and the system’s classification accuracy exceeded 0.95 for the four behaviors across all datasets(including original and simulated misalignment datasets).Sensitivity(Sen)and PPV for all datasets were above 0.9.The study provides farmers and the dairy industry with a practical,energy-efficient system for continuously monitoring cattle behavior to enhance herd productivity while reducing labor costs.
基金supported by Austrian Federal Ministry for Digital and Economic Affairsthe National Foundation for Research,Technology and Developmentsupported using resources of the Vet Core Facility(Mass Spectrometry)of the University of Veterinary Medicine Vienna。
文摘Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G(IgG)content.Immediately after calving,colostrum samples were collected from 18 cows to measure IgG concentration.Based on colostrum IgG content,samples were classified through cluster analysis and were identified as poor,average,and excellent quality.The proteome was assessed with quantitative shotgun proteomics;abundance data were compared among the colostrum types;enrichment analysis of metabolic processes and proteins classes was performed as well.We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.Results On average,428 proteins were identified per sample,which belonged mainly to cellular process,biological regulation,response to stimulus,metabolic process,and immune system process.Most abundant proteins were complement C3(Q2UVX4),alpha-S1-casein(P02662),Ig-like domain-containing protein(A0A3Q1M032),albumin(A0A140T897),polymeric immunoglobulin receptor(P81265),lactotransferrrin(P24627),and IGHG1*01(X167014).Colostrum of excellent quality had greater(P<0.05)abundance of serpin A3-7(A2I7N3),complement factorl(A0A3Q1 MIF4),lipocalin/cytosolic fatty-acid binding domain-containing protein(A0A3Q1 MRQ2),complement C3(E1B805),complement component 4 binding protein alpha(A0AAF6ZHP5),and complement component C6(F1MM86).However,colostrum of excellent quality had lower(P<0.05)abundance of HGF activator(E1BCW0),alpha-S1-casein(P02662),and xanthine dehydrogenase/oxidase(P80457).This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway,complement activation,complement activation classical pathway,humoral immune response,leukocyte mediated immunity,and negative regulation of endopeptidase activity in excellent-quality colostrum.Additionally,some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of calves(P<0.05;r≥0.57).Conclusions This study provides new insights into the bovine colostrum proteome,demonstrating associations between IgG levels and the abundance of other proteins,as well as the enrichment of metabolic processes related to innate immune response.Thus,results suggest that the colostrum proteomic profile is associated with the content of IgG.Future research should deeply explore the association of these findings with pre-calving nutrition status and blood composition of the cow,and with passive immunity transfer to the calf.
基金Supported by the National Natural Science Foundation of China(31601141)。
文摘A lactating dairy cow mammary epithelial cell(DCMEC)model is useful for studying milk synthesis and lactation in the dairy cow mammary gland.However,the cells can only maintain their original characteristics for a limited period in vitro.Thus,the proliferative capacity and lactation pattern of subcultured DCMECs need to be characterized.In the present study,subcultured DCMECs appeared to proliferate without changes in morphology or growth pattern up to the 12th passage.Subculturing had no obvious effect on the lactation capacity of the subcultured DCMEC up to the 10th passage in vitro.These results indicated that the DCMEC line could be subpassaged up to 10 times and still maintain its original milk synthesis characteristics.Moreover,DCMECs were frozen using different methods,and the method of placing the vials in the foam box was determined after cryopreservation with the best adherence of 91.2%.This method was recommended for long-term cryopreservation for DCMECs.
基金Supported by The Three Vertical Basic Cultivation Project of Heilongjiang Bayi Agricultural University(ZRCPY202314).
文摘To address the issue of low recognition accuracy for eight types of behaviors including standing,walking,drinking,lying,eating,mounting,fighting and limping in complex multi-cow farm environments,a multi-target cow behavior recognition method based on an improved YOLOv11n algorithm was proposed.The detection capability for small targets in images was enhanced by incorporating a DASI module into the backbone network and a MDCR module into the neck network,based on YOLOv11.The improved YOLOv11 algorithm increased the mean average precision from the original 89.5%to 93%,with particularly notable improvements of 8.7%and 6.3%in the average precision for recognizing drinking and walking behaviors,respectively.These results fully demonstrate that the proposed method enhances the model s ability to recognize cow behaviors.
文摘Cow milk protein allergy(CMPA)is a prevalent food allergy in infancy.It often presents with symptoms that overlap with other conditions,such as gastroeso-phageal reflux disease,lactose intolerance,food protein-induced enterocolitis syndrome,and eosinophilic esophagitis.This diagnostic overlap makes distin-guishing CMPA from its mimics difficult,resulting in potential misdiagnoses and unnecessary dietary restrictions.This review aims to comprehensively analyze CMPA and its mimicking conditions,highlighting their clinical presentations,diagnostic approaches,and management strategies to enhance diagnostic accu-racy and optimize patient care.A systematic literature search was conducted using PubMed,Scopus,Web of Science,and Google Scholar,focusing on studies published within the last 20 years.Articles addressing CMPA and its mimicking conditions were selected,with data synthesized into comparative analyses of diagnostic methods and management strategies.Accurate differentiation between CMPA and its mimics requires a thorough clinical evaluation supported by diagnostic tests such as skin prick tests,serum-specific IgE,and oral food challenges.Misdiagnosis can lead to nutritional deficiencies,psychological stress,and increased healthcare costs.Emerging diagnostic technologies,including component-resolved diagnostics and cytokine profiling,offer promising avenues for improving accuracy.A multidisciplinary approach involving pediatricians,allergists,and dietitians is essential for precise diagnosis and effective management.Ongoing research and education are crucial to enhancing clinical outcomes and reducing the burden on families.
文摘[Objective] The research aimed to explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein cow.[Method] 36 Holstein cows with similar birth order and milk yield were equally and randomly divided into 3 groups which were control group, Chinese medicine Ⅰ group and Chinese medicine Ⅱ group. In control group, cows were fed with basic feed, while in Chinese medicine Ⅰ group, cows were fed with basic feed containing 4% compound Chinese herb additive and in Chinese medicine Ⅱ group, cows were fed with basic feed containing 3% compound Chinese herb additive.All cows in the 3 groups were analyzed comparatively.[Result] Adding some compound Chinese herb additive into basic feed would increase milk yield and anti-heat stress ability of Holstein cow,besides,it also could decrease cow diseases such as cow mastitis.Chinese medicine Ⅰ would generate the highest economic benefit of practical production.[Conclusion] Adding Chinese herb additive into basic feed is an effective way for reliefing heat stress and increasing milk yield.
基金Supported by National Science and Technology Support Program"Key Technology Integration Research and Demonstration in the High-efficiency Production Mode of Farmland Cycle "(2007BAD89B16)~~
文摘[Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperature,moisture,pH value,crude fiber,TOC,TN,GI during composting.[Result] The results showed that adding complex enzymes could accelerate degradation of organic matter in pre-composting period.Crude fiber of 2.0%,.1.5% enzyme treatment group and control group decreased 49.6%,47% and 29.1% respectively,TOC decreased 41.7%,35.3% and 21.1%,TN decreased 32.6%,26.8% and 19.2%.2.0%,1.5% enzyme treatment groups could reach basic maturity degree at 30 d.[Conclusion] Composting cycle be shortened by adding complex enzymes,which was useful for maturity of cow dung compost.
基金Supported by Science and Technology Development Program of Shijiazhuang City(08150132A)China Spark Program(2012GA6200025)~~
文摘[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided into four groups, and were fed with forage supplemented with 0 （control group）, 0.2%, 0.4% and 0.6% Zengrujianniusan for 60 d. During this period, the contents of fat, protein and non-fat solid in milk were measured every 20 d. [Result] The milk production of the cows whose forage was added with 0.2 %, 0.4 % and 0.6% Zengrujianniusan was 4.02%, 12.50%, 14.00% higher than that of the control （P〉0.05）. The herbal feed additive had no significant influence on the contents of fat, milk and non-fat solid in milk, but significantly reduced the number of somatic cells. [Conclusion] The study will provide reference for developing feed additives which is safe and non-toxic to cows and their milk.
文摘Using a reversed-phase microemulsion polymerization method, polyoxometalates(POMs) CoW 11Ti loaded starch nanoparticles were prepared and structurally characterized by elemental analyses, IR, UV-Vis and ESR spectroscopy. The particle size of CoW 11Ti/starch was estimated by transmission electron microscope(TEM) and the size ranges by a 1000HSA MALVIRN Zetasizer instrument. The result shows that the polyoxometalate retained the parent structure after encapsulated by starch microspheres, which are able to enhance the stability and antitumoral activity of POMs and decrease the toxicity of POMs as well.
文摘为了探究过瘤胃蛋氨酸(Rumen-protected methionine,RPM)对泌乳高峰期奶牛生产性能和全肠道消化率的影响,试验选取16头处于泌乳高峰期,体重、生理状况相似的健康荷斯坦奶牛,随机分为2组。对照组每日饲喂基础TMR日粮,RPM组每头牛在基础日粮的基础上,每日单独补饲15 g RPM。结果表明:(1)泌乳高峰期奶牛日粮中添加RPM不影响体重、体况评分和干物质采食量(DMI)(P>0.05);(2)添加RPM对奶牛的产奶量和乳成分影响不显著(P>0.05);(3)添加RPM有降低瘤胃丁酸含量的趋势(P=0.069);(4)添加RPM不影响奶牛的表观消化率(P>0.05);(5)RPM显著提高了奶牛血浆尿素氮和二氧化碳水平(P<0.05);(6)RPM对血液中蛋氨酸的含量没有影响,脯氨酸浓度显著降低(P<0.05),具有降低血浆精氨酸和天冬氨酸含量的趋势(0.05<P<0.10)。总体而言,泌乳高峰期奶牛补饲RPM可能通过提高蛋氨酸吸收率、氨基酸代谢和机体蛋白合成改善泌乳高峰期奶牛健康状况。
