Covalently linked phthalocyanine complexes were incorporated in bilayer lipid membranes(BLN) and deposited on SnO_2 transparent electrodes, Their photovoltages were measured and compared. It has been found that a more...Covalently linked phthalocyanine complexes were incorporated in bilayer lipid membranes(BLN) and deposited on SnO_2 transparent electrodes, Their photovoltages were measured and compared. It has been found that a more favorable orientation and closer proximity are attained in the diad compounds between the donor (phthalocyanine)-acceptor(anthraquinone) pair than in the simple compound for efficient light-induced charge separation and transfer. The triad compound is the best among all tested compounds.展开更多
This study aimed to improve the functional properties of egg white protein(EWP)by covalent modification of EWP with xanthan gum(XG)and gallic acid(GA)/tannic acid(TA)to form covalent complexes.The structure and functi...This study aimed to improve the functional properties of egg white protein(EWP)by covalent modification of EWP with xanthan gum(XG)and gallic acid(GA)/tannic acid(TA)to form covalent complexes.The structure and functional properties of EWP before and after modification were explored.The results showed that the grafting degree of EWP-GA-XG and EWP-TA-XG reached 37.23%and 44.01%respectively measured by the OPA method.The secondary structure of EWP became loose after reaction with GA/TA and XG,with a decrease in the content ofα-helix and an increase in theβ-sheet content,thus contributing to the improvement of its functional properties.The DPPH and ABTS radical scavenging rate and iron reduction ability of EWP-GA-XG(44.94%,56.38%,18.29 mg VC/μmol)and EWP-TA-XG(85.45%,88.39%,39.26 mg VC/μmol)complexes were significantly improved compared with EWP(7.90%,12.94%,0.12 mg VC/μmol).The emulsion activity index and emulsion stability index of EWP-GA-XG(10.05 m^(2)/g,96.43%)and EWP-TA-XG(11.04 m^(2)/g,96.53%)complexes were also improved as compared to those of EWP(5.94 m^(2)/g,51.45%).Moreover,the peak transition temperatures of EWP-GA-XG(138.09◦C)and EWP-TA-XG(143.96◦C)complexes were higher than that of EWP(125.00◦C).This study may provide the theoretical basis for improving the functional properties of EWP and expanding its application in the field of food.展开更多
DNA topoisomerases are essential nuclear enzymes in correcting topological DNA errors and maintaining DNA integrity.Topoisomerase inhibitors are a significant class of cancer chemotherapeutics with a definite curative...DNA topoisomerases are essential nuclear enzymes in correcting topological DNA errors and maintaining DNA integrity.Topoisomerase inhibitors are a significant class of cancer chemotherapeutics with a definite curative effect.Natural products are a rich source of lead compounds for drug discovery,including anti-tumor drugs.In this study,we found that narciclasine(NCS),an amaryllidaceae alkaloid,is a novel inhibitor of topoisomerase I(topo I).Our data demonstrated that NCS inhibited topo I activity and reversed its unwinding effect on p-HOT DNA substrate.However,it had no obvious effect on topo II activity.The molecular mechanism of NCS inhibited topo I showed that NCS did not stabilize topo-DNA covalent complexes in cells,indicating that NCS is not a topo I poison.A blind docking result showed that NCS could bind to topo I,suggesting that NCS might be a topo I suppressor.Additionally,NCS exhibited a potent anti-proliferation effect in various cancer cells.NCS arrested the cell cycle at G_(2)/M phase and induced cell apoptosis.Our study reveals the antitumor mechanisms of NCS and provides a good foundation for the development of anti-cancer drugs based on topo I inhibition.展开更多
文摘Covalently linked phthalocyanine complexes were incorporated in bilayer lipid membranes(BLN) and deposited on SnO_2 transparent electrodes, Their photovoltages were measured and compared. It has been found that a more favorable orientation and closer proximity are attained in the diad compounds between the donor (phthalocyanine)-acceptor(anthraquinone) pair than in the simple compound for efficient light-induced charge separation and transfer. The triad compound is the best among all tested compounds.
基金supported financially by the National Natural Science Foundation of China(No.22078143)Jiangxi Provincial Finance Science and Technology Special“Contract System”Demonstration Project(No.ZBG20230418035)the Research Project of State Key Laboratory of Food Science and Resources,Nanchang University(No.SKLFZZB-202331).
文摘This study aimed to improve the functional properties of egg white protein(EWP)by covalent modification of EWP with xanthan gum(XG)and gallic acid(GA)/tannic acid(TA)to form covalent complexes.The structure and functional properties of EWP before and after modification were explored.The results showed that the grafting degree of EWP-GA-XG and EWP-TA-XG reached 37.23%and 44.01%respectively measured by the OPA method.The secondary structure of EWP became loose after reaction with GA/TA and XG,with a decrease in the content ofα-helix and an increase in theβ-sheet content,thus contributing to the improvement of its functional properties.The DPPH and ABTS radical scavenging rate and iron reduction ability of EWP-GA-XG(44.94%,56.38%,18.29 mg VC/μmol)and EWP-TA-XG(85.45%,88.39%,39.26 mg VC/μmol)complexes were significantly improved compared with EWP(7.90%,12.94%,0.12 mg VC/μmol).The emulsion activity index and emulsion stability index of EWP-GA-XG(10.05 m^(2)/g,96.43%)and EWP-TA-XG(11.04 m^(2)/g,96.53%)complexes were also improved as compared to those of EWP(5.94 m^(2)/g,51.45%).Moreover,the peak transition temperatures of EWP-GA-XG(138.09◦C)and EWP-TA-XG(143.96◦C)complexes were higher than that of EWP(125.00◦C).This study may provide the theoretical basis for improving the functional properties of EWP and expanding its application in the field of food.
基金the National Natural Science Foundation of China(Grant Nos.21907044,81460559 and 82160697)Yunnan Fundamental Research Projects(Grant Nos.202101AT070155 and 202201AS070086)+2 种基金Basic Research Plan of Yunnan Provincial Science and Technology Department-Kunming Medical University(Grant Nos.202101AY070001-011,202201AY070001-003 and 202101AY070001-041)the Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province(Hongyu Zhou,Dandan Liu),Yunnan Academician Expert Workstation(Grant No.202305AF150054)Basic Research Project of Yunnan Provincial Department of Education(Grant No.2022J0213).
文摘DNA topoisomerases are essential nuclear enzymes in correcting topological DNA errors and maintaining DNA integrity.Topoisomerase inhibitors are a significant class of cancer chemotherapeutics with a definite curative effect.Natural products are a rich source of lead compounds for drug discovery,including anti-tumor drugs.In this study,we found that narciclasine(NCS),an amaryllidaceae alkaloid,is a novel inhibitor of topoisomerase I(topo I).Our data demonstrated that NCS inhibited topo I activity and reversed its unwinding effect on p-HOT DNA substrate.However,it had no obvious effect on topo II activity.The molecular mechanism of NCS inhibited topo I showed that NCS did not stabilize topo-DNA covalent complexes in cells,indicating that NCS is not a topo I poison.A blind docking result showed that NCS could bind to topo I,suggesting that NCS might be a topo I suppressor.Additionally,NCS exhibited a potent anti-proliferation effect in various cancer cells.NCS arrested the cell cycle at G_(2)/M phase and induced cell apoptosis.Our study reveals the antitumor mechanisms of NCS and provides a good foundation for the development of anti-cancer drugs based on topo I inhibition.
