Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectro...Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectroscopic evidents.展开更多
Four unreported monoterpene indole alkaloids,tabernaecorymines B−E(1−4),together with twenty-one known indole alkaloids(5−25)were obtained from the stem bark of Tabernaemontana corymbosa.Their structures and absolute ...Four unreported monoterpene indole alkaloids,tabernaecorymines B−E(1−4),together with twenty-one known indole alkaloids(5−25)were obtained from the stem bark of Tabernaemontana corymbosa.Their structures and absolute configurations were elucidated by extensive spectroscopy,quantum chemical calculations,DP4+probability analyses and Mo2(OAc)4-induced electronic circular dichroism experiment.The antibacterial and antifungal activities of these compounds were evaluated and some of them showed significant activity against Staphylococcus aureus,Bacillus subtilis,Streptococcus dysgalactiae and Candida albicans.展开更多
The present study,antioxidant,enzyme inhibitory,anti-inflammatory and antimicrobial activity of isolated compounds such as Decussatin(1),Gentiacaulein(2),Swertianin(3),1,8-dihydroxy-2,6-dimethoxy xanthone(methylswerti...The present study,antioxidant,enzyme inhibitory,anti-inflammatory and antimicrobial activity of isolated compounds such as Decussatin(1),Gentiacaulein(2),Swertianin(3),1,8-dihydroxy-2,6-dimethoxy xanthone(methylswertianin)(4)8-hydroxy-1,2,4,6-tetramethoxyxanthone(5)and 1,2-dihydroxy-6-methoxyxanthone-8-O--d-xylopyranosyl(6)were investigated using an in vitro model.Results of antioxidant studies revealed that the compound 6 possessed an efficient 2,2-diphenyl-1-picryl-hydrazyl(DPPH•)(IC5007.19±4.56μmol/mL),2,2-azinobis(3-ethylbenzothiozoline-6-sulfonic acid)(ABTS•^+)(42.62±0.25 mmol/L TE/g),superoxide(57.89±3.45μmol/mL),nitric oxide(18.45±1.23μmol/mL)and hydroxyl(12.13±2.76μmol/mL)radical scavenging activities,ferric reducing antioxidant power(14.76±0.10 molar Fe(II)/g),metal chelating(213.85±27.18 mg EDTA/g)ability.Compounds 6 and 3 exhibited significant anti-proliferative activity.Compound 6 displayed strongest antibacterial activity against Streptococcus pneumoniae and Escherichia coli with MIC value of 3.90μg/mL and 21.21±0.25 and 20.27±0.11 mm zone of inhibition at 25μg/mL concentration respectively.In the membrane stabilization and protein denaturation test 3 was the most potent with an IC50 value of 12.57,18.75μmol/mL respectively.展开更多
Objective:To evaluate the acute toxicity and to investigate the effect of Premna corymbosa ethanolic extract(PCEE) at doses of 200 and 400 mg /kg body weight in acute and chronic models of inflammation in experimental...Objective:To evaluate the acute toxicity and to investigate the effect of Premna corymbosa ethanolic extract(PCEE) at doses of 200 and 400 mg /kg body weight in acute and chronic models of inflammation in experimental animals.Methods:In the acute toxicity study,a single dose of PCEE of 2 000 mg/kg body weight,p.o.was administered and observed for 48 h.In acute models as egg albumin induced paw edema and chronic model as cotton pellet methods was followed. Results:In acute models,egg albumin induced paw edema PCEE significantly(P【0.01) inhibited the edema formation.In chronic model,cotton pellet induced granuloma formation in rats PCEE significantly(P【0.01) reduced the granuloma formation with percentage inhibition of 35.17%and 50.38%respectively.Conclusions:The present study establishes the antiinflammatory activity of Premna corymbosa leaves.展开更多
The study conducted a field survey of the invasive plant Oxalis corymbosa DC. in Kunming and an invasion risk assessment was carried out. The results showed that the invasion risk of Oxalis corymbosa DC. in four distr...The study conducted a field survey of the invasive plant Oxalis corymbosa DC. in Kunming and an invasion risk assessment was carried out. The results showed that the invasion risk of Oxalis corymbosa DC. in four districts of Kunming was 1.9 in Wuhua District, 2.05 in Panlong District, 0.6 in Xishan District, 1.45 in Guandu District and 0.6 in downtown area, among which Panlong District had the highest invasion risk. Schools(1.9) > Parks(1.45) > Residential communities(0.6). In addition, it was found in the survey that the Oxalis latifolia as a quarantine weed had a sporadic distribution in Yunnan University of Finance and Economics, so it is necessary to pay attention to prevention and control to avoid the harm caused by escape and spread.展开更多
Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabi...Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.展开更多
Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperi...Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperitoneal injection of CCl_4,1 mL/kg body weight for every 72 h.The ethanolic extract of Premna corymbosa leaves were administrated at doses of 200 & 400 mL/kg body weight, p.o.,daily for 14 days.The hepatotoxicity and its prevention was assessed by serum markers like serum alkaline phosphatase(SALP),serum triglycerides(STG),serum total protein(STP), serum cholesterol(SC),and liver wet weight and histopathological studies of the liver.Results:In treatment with the ethanolic extract,the toxic effect of CCl_4 was controlled significantly(P<0.01) by restoration of the levels of biochemical parameters as compared to normal and standard drug silymarin treated groups.The liver weight was reduced by the ethanolic extract treated groups. The histopathology of the liver sections evidenced the hepatoprotective activity.Conclusion:The ethanolic extract of the leaves of Premna corymbosa possess significant acute hepatoprotective activity.Premna corymbosa can be recommended for the liver disorders.展开更多
Over centuries, Sub-Saharan Africans have been using <i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</sp...Over centuries, Sub-Saharan Africans have been using <i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> leaf extract during labour with the claims that it aids and facilitates childbirth among others. This study aimed at preliminary investigating the phytochemical constituent as well as the safety. Fresh plant (leaves) specimen was harvested and identified. Aqueous </span><i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> leaf extraction was carried out and used for phytochemical evaluation and toxicity study. Thirteen (13) adult Wistar rats were divided into 3 groups of 3 animals (n = 3) each for the first phase of the toxicity study and treated with doses (10, 100 and 1000) mg/kg respectively and observed for 48 hours. Four groups consisting of 1 animal each (n = 1) were used for the second phase and treated with doses of (140, 225, 370 and 600) mg/kg respectively. Phytochemical screening revealed the presence of carbohydrates, alkaloids, flavonoids, tannins, saponins, cardiac glycosides, sterol, triterpenes and anthraquinones in the extract. The LD</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> of the extract was found to be 471.17 mg/kg. The results of this study show that the aqueous leaf extract of </span><i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> is slightly toxic with potentials for biological activities from phytochemicals present.</span>展开更多
Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the curren...Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the current system was ineffective.Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.Thin layer chromatography(TLC)was employed to separate HC from Hedyotis diffusa(Bai Hua She She Cao,白花蛇舌草,HD).Simultaneously,high-performance liquid chromatography(HPLC)was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A.Regarding the issue of systematicness,concentrations of water,total ash,acid-insoluble ash,heavy metals(including lead,arsenic,mercury,cadmium and copper)and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia.Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue.Three characteristic blue fluorescent spots were observed in the HC samples by TLC.There were five distinct characteristic peaks in HC but not in HD,and hedyotiscone A was the sharpest characteristic peak.The generated linear equation of hedyotiscone A was Y=3.15×108X+312.04(r2=1.000)between 0.00005270 and 0.002108 mg/mL,and the average recovery was 100.42%.Precision[relative standard deviation(RSD)0.92%],repeatability(RSD 0.14%),and recovery(RSD 0.85%)met the requirements for quantitative analysis.Hedyotiscone A concentrations ranged from 0.00017%~0.00113%across different habitats.Concentrations of water,ash and acid-insoluble ash were 8.80%~11.2%,7.00%~15.3%and 0.440%~7.70%,respectively.Concentrations of Pb,As,Hg,Cd and Cu were 1.60~4.60,0.650~1.60,0.0300~0.100,0.240~0.690 and 10.1~16.6μg/g,respectively.Pesticide residues were less than 0.00100 ppm.Conclusion The quality control system can effectively distinguish HC from HD,so as to comprehensively control the quality of HC,to provide references for the clinical use of HC.展开更多
目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 ...目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 :槲皮素 7 O α L 阿拉伯糖 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 7 O α L arabinosyl 3 O β D 6″ acetylglucopyranoside ,1 ) ;山奈酚 7 O α L 阿拉伯糖 3 O β D 吡喃葡糖苷 (kaempferol 7 O α L arabinosyl 3 O β D glucopyranoside ,2 ) ;槲皮素 3 O β D 吡喃葡糖苷 (quercetin 3 O β D glucopyranoside ,3) ;槲皮素 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 3 O β D 6″ acetylglucopyranoside,4 )。 结论 化合物 1为新化合物 。展开更多
文摘Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectroscopic evidents.
基金supported by the National Natural Science Foundation of China(Nos.31900290 and 32000274)Yunnan Province Young Academic Project(No.202105AC160078)+3 种基金the Scholar of Yunling(No.YNWR-YLXZ-2019-019)the Basic Project in Yunnan Province(No.202101AU070069)the Development and Application of Biotic Resource in Yunnan Province(No.202002AA100007)Yunnan Key Laboratory of Pharmacology for Natural Products Fund Project.
文摘Four unreported monoterpene indole alkaloids,tabernaecorymines B−E(1−4),together with twenty-one known indole alkaloids(5−25)were obtained from the stem bark of Tabernaemontana corymbosa.Their structures and absolute configurations were elucidated by extensive spectroscopy,quantum chemical calculations,DP4+probability analyses and Mo2(OAc)4-induced electronic circular dichroism experiment.The antibacterial and antifungal activities of these compounds were evaluated and some of them showed significant activity against Staphylococcus aureus,Bacillus subtilis,Streptococcus dysgalactiae and Candida albicans.
文摘The present study,antioxidant,enzyme inhibitory,anti-inflammatory and antimicrobial activity of isolated compounds such as Decussatin(1),Gentiacaulein(2),Swertianin(3),1,8-dihydroxy-2,6-dimethoxy xanthone(methylswertianin)(4)8-hydroxy-1,2,4,6-tetramethoxyxanthone(5)and 1,2-dihydroxy-6-methoxyxanthone-8-O--d-xylopyranosyl(6)were investigated using an in vitro model.Results of antioxidant studies revealed that the compound 6 possessed an efficient 2,2-diphenyl-1-picryl-hydrazyl(DPPH•)(IC5007.19±4.56μmol/mL),2,2-azinobis(3-ethylbenzothiozoline-6-sulfonic acid)(ABTS•^+)(42.62±0.25 mmol/L TE/g),superoxide(57.89±3.45μmol/mL),nitric oxide(18.45±1.23μmol/mL)and hydroxyl(12.13±2.76μmol/mL)radical scavenging activities,ferric reducing antioxidant power(14.76±0.10 molar Fe(II)/g),metal chelating(213.85±27.18 mg EDTA/g)ability.Compounds 6 and 3 exhibited significant anti-proliferative activity.Compound 6 displayed strongest antibacterial activity against Streptococcus pneumoniae and Escherichia coli with MIC value of 3.90μg/mL and 21.21±0.25 and 20.27±0.11 mm zone of inhibition at 25μg/mL concentration respectively.In the membrane stabilization and protein denaturation test 3 was the most potent with an IC50 value of 12.57,18.75μmol/mL respectively.
文摘Objective:To evaluate the acute toxicity and to investigate the effect of Premna corymbosa ethanolic extract(PCEE) at doses of 200 and 400 mg /kg body weight in acute and chronic models of inflammation in experimental animals.Methods:In the acute toxicity study,a single dose of PCEE of 2 000 mg/kg body weight,p.o.was administered and observed for 48 h.In acute models as egg albumin induced paw edema and chronic model as cotton pellet methods was followed. Results:In acute models,egg albumin induced paw edema PCEE significantly(P【0.01) inhibited the edema formation.In chronic model,cotton pellet induced granuloma formation in rats PCEE significantly(P【0.01) reduced the granuloma formation with percentage inhibition of 35.17%and 50.38%respectively.Conclusions:The present study establishes the antiinflammatory activity of Premna corymbosa leaves.
基金Sponsored by Scientific Research Fund Project of Yunnan provincial Department of Education(2019J1096)
文摘The study conducted a field survey of the invasive plant Oxalis corymbosa DC. in Kunming and an invasion risk assessment was carried out. The results showed that the invasion risk of Oxalis corymbosa DC. in four districts of Kunming was 1.9 in Wuhua District, 2.05 in Panlong District, 0.6 in Xishan District, 1.45 in Guandu District and 0.6 in downtown area, among which Panlong District had the highest invasion risk. Schools(1.9) > Parks(1.45) > Residential communities(0.6). In addition, it was found in the survey that the Oxalis latifolia as a quarantine weed had a sporadic distribution in Yunnan University of Finance and Economics, so it is necessary to pay attention to prevention and control to avoid the harm caused by escape and spread.
文摘Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.
文摘Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperitoneal injection of CCl_4,1 mL/kg body weight for every 72 h.The ethanolic extract of Premna corymbosa leaves were administrated at doses of 200 & 400 mL/kg body weight, p.o.,daily for 14 days.The hepatotoxicity and its prevention was assessed by serum markers like serum alkaline phosphatase(SALP),serum triglycerides(STG),serum total protein(STP), serum cholesterol(SC),and liver wet weight and histopathological studies of the liver.Results:In treatment with the ethanolic extract,the toxic effect of CCl_4 was controlled significantly(P<0.01) by restoration of the levels of biochemical parameters as compared to normal and standard drug silymarin treated groups.The liver weight was reduced by the ethanolic extract treated groups. The histopathology of the liver sections evidenced the hepatoprotective activity.Conclusion:The ethanolic extract of the leaves of Premna corymbosa possess significant acute hepatoprotective activity.Premna corymbosa can be recommended for the liver disorders.
文摘Over centuries, Sub-Saharan Africans have been using <i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> leaf extract during labour with the claims that it aids and facilitates childbirth among others. This study aimed at preliminary investigating the phytochemical constituent as well as the safety. Fresh plant (leaves) specimen was harvested and identified. Aqueous </span><i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> leaf extraction was carried out and used for phytochemical evaluation and toxicity study. Thirteen (13) adult Wistar rats were divided into 3 groups of 3 animals (n = 3) each for the first phase of the toxicity study and treated with doses (10, 100 and 1000) mg/kg respectively and observed for 48 hours. Four groups consisting of 1 animal each (n = 1) were used for the second phase and treated with doses of (140, 225, 370 and 600) mg/kg respectively. Phytochemical screening revealed the presence of carbohydrates, alkaloids, flavonoids, tannins, saponins, cardiac glycosides, sterol, triterpenes and anthraquinones in the extract. The LD</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> of the extract was found to be 471.17 mg/kg. The results of this study show that the aqueous leaf extract of </span><i><span style="font-family:Verdana;">Sida</span></i> <i><span style="font-family:Verdana;">corymbosa</span></i><span style="font-family:Verdana;"> is slightly toxic with potentials for biological activities from phytochemicals present.</span>
基金support from the National Science foundation of China(No.81503041)the Science Research Projects of Chinese Pharmacopoeia(No.Z18)+2 种基金the Science Research Projects of the Hunan Provincial Department of Education(No.17C1213)the Science Research Projects of the Hunan Provincial Department of Education(No.14C0860)and the Key Projects of the Changsha Municipal Science and Technology Bureau(No.K1406030-31)
文摘Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the current system was ineffective.Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.Thin layer chromatography(TLC)was employed to separate HC from Hedyotis diffusa(Bai Hua She She Cao,白花蛇舌草,HD).Simultaneously,high-performance liquid chromatography(HPLC)was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A.Regarding the issue of systematicness,concentrations of water,total ash,acid-insoluble ash,heavy metals(including lead,arsenic,mercury,cadmium and copper)and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia.Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue.Three characteristic blue fluorescent spots were observed in the HC samples by TLC.There were five distinct characteristic peaks in HC but not in HD,and hedyotiscone A was the sharpest characteristic peak.The generated linear equation of hedyotiscone A was Y=3.15×108X+312.04(r2=1.000)between 0.00005270 and 0.002108 mg/mL,and the average recovery was 100.42%.Precision[relative standard deviation(RSD)0.92%],repeatability(RSD 0.14%),and recovery(RSD 0.85%)met the requirements for quantitative analysis.Hedyotiscone A concentrations ranged from 0.00017%~0.00113%across different habitats.Concentrations of water,ash and acid-insoluble ash were 8.80%~11.2%,7.00%~15.3%and 0.440%~7.70%,respectively.Concentrations of Pb,As,Hg,Cd and Cu were 1.60~4.60,0.650~1.60,0.0300~0.100,0.240~0.690 and 10.1~16.6μg/g,respectively.Pesticide residues were less than 0.00100 ppm.Conclusion The quality control system can effectively distinguish HC from HD,so as to comprehensively control the quality of HC,to provide references for the clinical use of HC.
文摘目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 :槲皮素 7 O α L 阿拉伯糖 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 7 O α L arabinosyl 3 O β D 6″ acetylglucopyranoside ,1 ) ;山奈酚 7 O α L 阿拉伯糖 3 O β D 吡喃葡糖苷 (kaempferol 7 O α L arabinosyl 3 O β D glucopyranoside ,2 ) ;槲皮素 3 O β D 吡喃葡糖苷 (quercetin 3 O β D glucopyranoside ,3) ;槲皮素 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 3 O β D 6″ acetylglucopyranoside,4 )。 结论 化合物 1为新化合物 。
