Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of...Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC 50 values) were given. Results of measurements indicated that 2 h-LC 50 and 12 h-LC 50 values of formalin on P. coronatum were 59.00×10 -6 and 43.57×10 -6, while those of Lugol’s solutions were 90.13 and 67.84×10 -6 respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.展开更多
Kalapi(Kalappia celebica)is an endemic legume of Sulawesi and has been included in the endangered category since the early 1980s.Conservation of the species is possible through ex situ culture techniques.Arbuscular my...Kalapi(Kalappia celebica)is an endemic legume of Sulawesi and has been included in the endangered category since the early 1980s.Conservation of the species is possible through ex situ culture techniques.Arbuscular mycorrhizal fungi(AMF)can accelerate plant growth which in turn supports the conservation of endangered species.This study aimed to assess the efficacy of local AMF to accelerate the growth of kalapi and increase nutrient uptake in kalapi grown in gold mine tailing media.There were three AMF treatments,Glomus claroideum,Glomus coronatum,and a mixture of both,plus the control.Each treatment was replicated three times,each consisting of five plants.The results show that the highest AMF colony was obtained by kalapi seedlings inoculated with Glomus coronatum and the mixture of AMF.The range of mycorrhizae inoculation effect values was 59.7–71.3%.AMF inoculation increased growth and dry weight of 4-month-old seedlings compared to controls.Dry and total weights of kalapi inoculated with G.coronatum were significantly different from those inoculated with the AMF mixture.However,they are not significantly different from kalapi inoculated with G.claroideum.The results also show that AMF increased nitrogen and phosphorous uptake by the roots,as well as nitrogen,phosphorous,potassium,manganese and iron by the shoots.All AMF treatments decreased potassium uptake in the roots,except in kalapi inoculated with G.coronatum.The AMF mixture decreased iron contents the roots by 15%.AMF can be developed into biofertilizer to support the conservation of kalapi in tropical Indonesia.展开更多
基金This work is supported by the Natural Science Foundation of China(No.40206021).
文摘Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC 50 values) were given. Results of measurements indicated that 2 h-LC 50 and 12 h-LC 50 values of formalin on P. coronatum were 59.00×10 -6 and 43.57×10 -6, while those of Lugol’s solutions were 90.13 and 67.84×10 -6 respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.
基金supported by the funding support(No.171.SP2H/LT/DRPM/2019)from Directorate General of Resource,Science,Technology,Higher Education,Ministry of Research,Technology of the Republic of Indonesia。
文摘Kalapi(Kalappia celebica)is an endemic legume of Sulawesi and has been included in the endangered category since the early 1980s.Conservation of the species is possible through ex situ culture techniques.Arbuscular mycorrhizal fungi(AMF)can accelerate plant growth which in turn supports the conservation of endangered species.This study aimed to assess the efficacy of local AMF to accelerate the growth of kalapi and increase nutrient uptake in kalapi grown in gold mine tailing media.There were three AMF treatments,Glomus claroideum,Glomus coronatum,and a mixture of both,plus the control.Each treatment was replicated three times,each consisting of five plants.The results show that the highest AMF colony was obtained by kalapi seedlings inoculated with Glomus coronatum and the mixture of AMF.The range of mycorrhizae inoculation effect values was 59.7–71.3%.AMF inoculation increased growth and dry weight of 4-month-old seedlings compared to controls.Dry and total weights of kalapi inoculated with G.coronatum were significantly different from those inoculated with the AMF mixture.However,they are not significantly different from kalapi inoculated with G.claroideum.The results also show that AMF increased nitrogen and phosphorous uptake by the roots,as well as nitrogen,phosphorous,potassium,manganese and iron by the shoots.All AMF treatments decreased potassium uptake in the roots,except in kalapi inoculated with G.coronatum.The AMF mixture decreased iron contents the roots by 15%.AMF can be developed into biofertilizer to support the conservation of kalapi in tropical Indonesia.
