The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predom...The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predominantly agricultural state in Brazil and has many rivers and permanent and seasonal lakes, in which the Hedychium coronarium, an aquatic plant, is found the swampy environments. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA and UV detection for the presence of thiamethoxam. The thiamethoxam was recovered from these samples at rates ranging from 81.16% - 99.93%. The coefficient of variation in the quantitative analysis of the thiamethoxam was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with determination coefficients of r2 = 0.9992 (water and soil) and r2 = 0.9990 (leaves and rhizomes) for the linear range of the analytical calibration curves of the samples. The detection limit was 0.36 μg.L-1 and quantification limit was 1.2 μg.L-1 for thiamethoxam. The method was considered sensitive for quantification of the thiamethoxam in water, soil and rhizomes and leaves of Hedychium coronarium.展开更多
为了优化大孔吸附树脂分离纯化茼蒿总黄酮(TFC)的工艺条件,试验以TFC吸附量和回收率为指标,考察5种大孔吸附树脂静态、动态的吸附及解吸附性能。优选影响因素——上样浓度,上样p H值,洗脱浓度和洗脱流速4种试验条件,测定大孔树脂的泄露...为了优化大孔吸附树脂分离纯化茼蒿总黄酮(TFC)的工艺条件,试验以TFC吸附量和回收率为指标,考察5种大孔吸附树脂静态、动态的吸附及解吸附性能。优选影响因素——上样浓度,上样p H值,洗脱浓度和洗脱流速4种试验条件,测定大孔树脂的泄露曲线和洗脱曲线。研究发现,D-101大孔树脂对TFC的吸附纯化显示出最佳的性能。泄露曲线显示:上样体积泄漏点60 m L,饱和点210 m L。洗脱曲线显示:体积分数95%乙醇溶液80 m L洗脱效果最好。响应面试验方差分析显示:上样p H对TFC回收率影响差异达到显著水平,而上样浓度、洗脱浓度和洗脱速度3个因素对TFC回收率影响差异不显著。通过回归方程模拟预测最优纯化条件组合——上样浓度0.86mg/m L,p H=6,洗脱浓度74.27%,洗脱流速2.23 m L/min,TFC回收率由65.19%提升至87.36%。D-101大孔树脂对于TFC的分离纯化性能较好,适用于工业化推广。展开更多
文摘The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predominantly agricultural state in Brazil and has many rivers and permanent and seasonal lakes, in which the Hedychium coronarium, an aquatic plant, is found the swampy environments. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA and UV detection for the presence of thiamethoxam. The thiamethoxam was recovered from these samples at rates ranging from 81.16% - 99.93%. The coefficient of variation in the quantitative analysis of the thiamethoxam was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with determination coefficients of r2 = 0.9992 (water and soil) and r2 = 0.9990 (leaves and rhizomes) for the linear range of the analytical calibration curves of the samples. The detection limit was 0.36 μg.L-1 and quantification limit was 1.2 μg.L-1 for thiamethoxam. The method was considered sensitive for quantification of the thiamethoxam in water, soil and rhizomes and leaves of Hedychium coronarium.
文摘为了优化大孔吸附树脂分离纯化茼蒿总黄酮(TFC)的工艺条件,试验以TFC吸附量和回收率为指标,考察5种大孔吸附树脂静态、动态的吸附及解吸附性能。优选影响因素——上样浓度,上样p H值,洗脱浓度和洗脱流速4种试验条件,测定大孔树脂的泄露曲线和洗脱曲线。研究发现,D-101大孔树脂对TFC的吸附纯化显示出最佳的性能。泄露曲线显示:上样体积泄漏点60 m L,饱和点210 m L。洗脱曲线显示:体积分数95%乙醇溶液80 m L洗脱效果最好。响应面试验方差分析显示:上样p H对TFC回收率影响差异达到显著水平,而上样浓度、洗脱浓度和洗脱速度3个因素对TFC回收率影响差异不显著。通过回归方程模拟预测最优纯化条件组合——上样浓度0.86mg/m L,p H=6,洗脱浓度74.27%,洗脱流速2.23 m L/min,TFC回收率由65.19%提升至87.36%。D-101大孔树脂对于TFC的分离纯化性能较好,适用于工业化推广。
