[Objective] This study aimed to optimize the conditions for liquid culture of P. comucopiaeseeds. [Method] ＂P. cornucopiae 2＇ selected by our laboratory was used as the experimental strain in this study. Single fact...[Objective] This study aimed to optimize the conditions for liquid culture of P. comucopiaeseeds. [Method] ＂P. cornucopiae 2＇ selected by our laboratory was used as the experimental strain in this study. Single factor experiment in conjunction with Plackett-Burman （PB） design as well as Response Surface Methodology（RSM） based on Central Composite Design （CCD） were used to screen and optimize the eight factors influencing the technique for liquid culture of P. cornucopiae seeds. [Result] Firstly, the eight factors influencing the biomass of P. cornucopiae seeds in liquid culture were confirmed by means of single factor experiment; secondly, through Plackett-Burman experiment design, three main factors influencing the biomass of P. cornucopiae seeds in liquid culture were screened, namely the amount of added mannitol, the initial pH and culture temperature; lastly, the second order model, that is Y=-70.72＋2.44X3 ＋2.67X7 ＋3.72X8 ＋0.08X3 X7 -0.01X7 X8-0.05X32- 0.34X72-0.08X82, was obtained through Central Composite Design （CCD） along with Response Surface Methodology （RSM）. [Conclusion] The optimal conditions for the production of P. cornucopiae seeds in liquid culture were as follows： the amount of added mannitol of 28.17 g/L, the initial pH controlled at 5.90, 250 ml erlenmeyer flask filled with 100 ml of liquid medium, inoculum size of 5%（v/v）, rotation speed of 150 r/min, and gyratory culture at 23.97℃ for 5 days.展开更多
This study was carried out to investigate the therapeutic role of the ethanolic extract of Pleurotus cornucopiae on sodium arsenite induced nephrotoxicity in rats. Sodium arsenite at the dose of 8 mg•kg–1 bo...This study was carried out to investigate the therapeutic role of the ethanolic extract of Pleurotus cornucopiae on sodium arsenite induced nephrotoxicity in rats. Sodium arsenite at the dose of 8 mg•kg–1 body weight orally caused renal damage in rats as manifested by the significant rise in serum levels of serum urea, uric acid and creatinine level compared with control. Ethanolic extracts of P. cornucopiae (400 mg•kg–1 body weight per day) was administered orally for 30 days to sodium arsenite pre-treated rats. The results show significant decrease in the serum urea, uric acid and creatinine levels in comparison to the arsenic treated denotes the nephroprotective effect of P. cornucopiae against sodium arsenite induced toxicity. Furthermore, it also possesses antioxidant effect as lipid peroxidation (MDA) levels decreased in P. cornucopiae treated group in comparison to arsenic treated group. Thus, the present study reveals that P. cornucopiae possesses nephroprotective as well as antioxidant property against arsenic induced toxicity.展开更多
On April 1,the National Museum of China held its first exchange exhibition,the Art of Enlightenment.This exhibition was a joint event hosted with three German museums presenting nearly 600 exhibits that had
基金Supported by the Agricultural "Three Terms" Program in Jiangsu Province (sx (2009) 133)the Plan Program for Demonstration of Scientific and Technological Achievements in Agriculture of Lianyungang City(SF0902)~~
文摘[Objective] This study aimed to optimize the conditions for liquid culture of P. comucopiaeseeds. [Method] ＂P. cornucopiae 2＇ selected by our laboratory was used as the experimental strain in this study. Single factor experiment in conjunction with Plackett-Burman （PB） design as well as Response Surface Methodology（RSM） based on Central Composite Design （CCD） were used to screen and optimize the eight factors influencing the technique for liquid culture of P. cornucopiae seeds. [Result] Firstly, the eight factors influencing the biomass of P. cornucopiae seeds in liquid culture were confirmed by means of single factor experiment; secondly, through Plackett-Burman experiment design, three main factors influencing the biomass of P. cornucopiae seeds in liquid culture were screened, namely the amount of added mannitol, the initial pH and culture temperature; lastly, the second order model, that is Y=-70.72＋2.44X3 ＋2.67X7 ＋3.72X8 ＋0.08X3 X7 -0.01X7 X8-0.05X32- 0.34X72-0.08X82, was obtained through Central Composite Design （CCD） along with Response Surface Methodology （RSM）. [Conclusion] The optimal conditions for the production of P. cornucopiae seeds in liquid culture were as follows： the amount of added mannitol of 28.17 g/L, the initial pH controlled at 5.90, 250 ml erlenmeyer flask filled with 100 ml of liquid medium, inoculum size of 5%（v/v）, rotation speed of 150 r/min, and gyratory culture at 23.97℃ for 5 days.
文摘This study was carried out to investigate the therapeutic role of the ethanolic extract of Pleurotus cornucopiae on sodium arsenite induced nephrotoxicity in rats. Sodium arsenite at the dose of 8 mg•kg–1 body weight orally caused renal damage in rats as manifested by the significant rise in serum levels of serum urea, uric acid and creatinine level compared with control. Ethanolic extracts of P. cornucopiae (400 mg•kg–1 body weight per day) was administered orally for 30 days to sodium arsenite pre-treated rats. The results show significant decrease in the serum urea, uric acid and creatinine levels in comparison to the arsenic treated denotes the nephroprotective effect of P. cornucopiae against sodium arsenite induced toxicity. Furthermore, it also possesses antioxidant effect as lipid peroxidation (MDA) levels decreased in P. cornucopiae treated group in comparison to arsenic treated group. Thus, the present study reveals that P. cornucopiae possesses nephroprotective as well as antioxidant property against arsenic induced toxicity.
文摘On April 1,the National Museum of China held its first exchange exhibition,the Art of Enlightenment.This exhibition was a joint event hosted with three German museums presenting nearly 600 exhibits that had
