The research mainly analyzed effects of waxy corn/soybean intercropping on yields of the two crops, as well as agronomic characters, and the economic benefits of the mode. The results showed that although yields of wa...The research mainly analyzed effects of waxy corn/soybean intercropping on yields of the two crops, as well as agronomic characters, and the economic benefits of the mode. The results showed that although yields of waxy corn and soybean went down by 21.19% and 31.04% per unit area, land equivalent ratio（1.48） kept higher than 1, suggesting the intercropping improves land use rate. Besides, due to the practice of intercropping, many characters of waxy corn grew, but of soybean declined. The economic benefits from high to low were waxy corn/soybean intercropping, monoculture of waxy corn, and monoculture of soybean.展开更多
[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercroppin...[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercropping fields in Siyang County.[Methods]The trial comprised six herbicide treatments and one blank control,with investigations conducted to assess efficacy,safety,and yield.[Results]Each herbicide treatment effectively controlled weeds,demonstrated high safety,and enhanced the yields of both soybeans and corn.The combined application of soil sealing with stem and leaf spray exhibited superior overall weed control compared to soil sealing alone.At 28 d following stem and leaf spray,the plant control effect and fresh weight control effect against weeds in the combined treatment of soil sealing with stem and leaf spray all exceeded 89%.[Conclusions]This study offers technical support for advancing the practice of strip intercropping between corn and soybeans.展开更多
The soybean and corn strip compound planting technology is a crucial measure for improving land use efficiency and ensuring food security.This paper deeply analyzed the principles,advantages,and key technical aspects ...The soybean and corn strip compound planting technology is a crucial measure for improving land use efficiency and ensuring food security.This paper deeply analyzed the principles,advantages,and key technical aspects of this technology,including variety selection,planting pattern,sowing management,and field management.It also illustrated its application effectiveness through practical cases and proposed corresponding solutions to existing challenges in its promotion.This study provides theoretical support and practical reference for the widespread adoption and efficient application of this technology.展开更多
Struvite (MgNH<sub>4</sub>PO<sub>4</sub>·6H<sub>2</sub>O) produced synthetically from a stock solution of known phosphorus (P) and nitrogen (N) concentrations has been shown to...Struvite (MgNH<sub>4</sub>PO<sub>4</sub>·6H<sub>2</sub>O) produced synthetically from a stock solution of known phosphorus (P) and nitrogen (N) concentrations has been shown to be an effective, alternative fertilizer-P source for various crops, but little is known about the potential agronomic effectiveness of struvite created from an actual municipal wastewater source. The objective of this study was to evaluate the effects of soil [i.e., Creldon silt loam (Oxyaquic Fragiudalf) and Calloway silt loam (Aquic Fraglossudalf) series], fertilizer-P source [i.e., synthetically produced electrochemically precipitated struvite (ECSTsyn), real-wastewater-derived ECST (ECSTreal), chemically precipitated struvite (CPST), monoammonium phosphate (MAP), and an unamended control (UC)], and irrigation water type (i.e., tapwater and struvite-removed wastewater) on corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] growth and N, P, and magnesium (Mg) uptake in a 60-day, greenhouse potted-plant study. Crop growth and N, P, and Mg uptakes for the struvite treatments (i.e., CPST, ECSTsyn, and ECSTreal) were generally similar to or at least 1.2 times greater than MAP. The ECSTsyn material commonly had up to five times greater N, P, and Mg uptake in corn and soybean than any other fertilizer-P source. Struvite-removed wastewater resulted in at least 1.3 times lower dry matter and N, P, and Mg uptake than tapwater. Similar corn and soybean results from the struvite fertilizers among the various soil-water type combinations compared to MAP suggest that struvite generates similar crop responses as at least one widely used, commercially available, multi-nutrient fertilizer-P source.展开更多
Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin f...Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin first”strategy,corn straw is converted to valuable chemicals including lignin monomers,furfural and 5-methoxymethylfurfural via a two steps process.The key of this research lies in the development of a green and low-cost catalytic process utilizing magnetic Raney Ni catalyst and high boiling point ethylene glycol.The utilization of neat ethylene glycol as the sole slovent under atmospheric conditions obviates the need for additional additives,thereby facilitating the entire process to be conducted in glass flasks and rendering it highly convenient for scaling up.In the initial step,depolymerization of corn straw lignin resulted in a monomer yield of 18.1 wt%.Subsequently,in a dimethyl carbonate system,the carbohydrate component underwent complete conversion in a one-pot process,yielding furfural and 5-methoxymethylfurfural as the primary products with an impressive yield of 47.7%.展开更多
Background Reduction of the particle size of corn increases energy digestibility and concentrations of digestible and metabolizable energy.Pelleting may also reduce particle size of grain,but it is not known if there ...Background Reduction of the particle size of corn increases energy digestibility and concentrations of digestible and metabolizable energy.Pelleting may also reduce particle size of grain,but it is not known if there are interactions between particle size reduction and pelleting.The objective of this experiment was to test the hypothesis that particle size reduction and pelleting,separately or in combination,increase N balance,apparent total tract digestibility(ATTD)of fiber and fat,and net energy(NE)in corn-soybean meal diets fed to group-housed pigs.Methods Six corn-soybean meal-based diets were used in a 3×2 factorial design with 3 particle sizes of corn(i.e.,700,500,or 300μm)and 2 diet forms(i.e.,meal or pelleted).Pigs were allowed ad libitum access to feed and water.Twenty-four castrated male pigs(initial weight:29.52 kg;standard diviation:1.40)were allotted to the 6 diets using a 6×6 Latin square design with 6 calorimeter chambers(i.e.,4 pigs/chamber)and 6 periods.Oxygen consumption and CO_(2)and CH_(4)productions were measured during fed and fasting states and fecal and urine samples were collected.Results Regardless of particle size of corn,the ATTD of gross energy(GE),N,and acid-hydrolyzed ether extract(AEE),and the concentration of NE were greater(P<0.05)in pelleted diets than in meal diets.Regardless of diet form,the ATTD of GE,N,and AEE,and the concentration of NE were increased(linear;P<0.05)by reducing the particle size of corn,but the increase was greater in meal diets than in pelleted diets(interaction;P<0.05).Conclusions Both pelleting and reduction of corn particle size increased nutrient digestibility and NE,but increases were greater in meal diets than in pelleted diets.展开更多
To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subseq...To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.展开更多
Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop mor...Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop morphological development and increase yield.Here,a three-year study was performed to verify the feasibility of adjusting row spacing to further enhance yield in densely planted soybeans.Of three row-spacing configurations(40-40,20-40,and 20-60 cm)and two planting densities(normal 180,000 plants ha 1 and high 270,000 plants ha 1).The differences in canopy structure,plant morphological development,photosynthetic capacity and their impact on yield were analyzed.Row spacing configurations have a significant effect on canopy transmittance(CT).The 20-60 cm row spacing configuration increased CT and creates a favorable canopy light environment,in which plant height is reduced,while branching is promoted.This approach reduces plant competition,optimizes the developments of leaf area per plant,specific leaf area,leaf area development rate,leaf area duration and photosynthetic physiological indices(F_(v)/F_(m),ETR,P_(n)).The significant increase of 11.9%-34.2%in canopy apparent photosynthesis(CAP)is attributed to the significant optimization of plant growth and photosynthetic physiology through CT,an important contributing factor to yield increases.The yield in the 20-60 cm treatment is 4.0%higher than in equidistant planting under normal planting density,but 5.9%under high density,primarily driven by CAP and pod number.These findings suggest that suitable row spacing configurations optimize the light environment for plants,promote source-sink transformation in soybeans,and further improve yield.In practice,a 20-60 cm row spacing configuration could be employed for high-density soybean planting to achieve a more substantial yield gain.展开更多
A flower-like BiOBr photocatalyst(CS/BiOBr)was prepared by using the carbon material derived from corn straw(CS)as the carrier.The prepared composites were characterized by X-ray diffraction(XRD),Fourier transform inf...A flower-like BiOBr photocatalyst(CS/BiOBr)was prepared by using the carbon material derived from corn straw(CS)as the carrier.The prepared composites were characterized by X-ray diffraction(XRD),Fourier transform infrared(FIIR)spectra,scanning electron microscope(SEM),X-ray photoelectron spectra(XPS),and UV-Vis diffuse reflectance spectra(UV-Vis DRS).The SEM analyses indicate that the introduction of CS promotes the formation of a unique flower-like structure in BiOBr,which not only optimizes the efficiency of light capture but also increases the specific surface area of BiOBr.The bandgap of the composite was narrower compared with the pure BiOBr.The CS/BiOBr composites exhibited higher photocatalytic activity than pure CS and BiOBr under visible light irradiation,and a higher first-order reaction rate constant(k)of 0.0437 min-1 than BiOBr(0.0146 min^(-1)),and exhibited excellent stability and reusability during the cyclic run.The enhanced photocatalytic activity is attributed to the efficient separation of photoinduced electrons and holes.Superoxide radicals and holes were the major active species.展开更多
Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value....Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value.Despite these benefits,SBM contains various antigen proteins such as glycinin andβ-conglycinin,which account for approximately 70%of the total proteins of the SBM and reduce digestibility and damage intestinal function(Peng et al.,2018).展开更多
Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is...Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is intercropped with different soybean varieties.In this study,field experiments were conducted to compare the growth and yield of two soybean(Glycine max)varieties,BD2 and YC03-3,and one maize(Zea mays)variety,Huazhen,in mono-cropped and intercropped cultures.The plant biomass and N content of both crops in BD2/maize intercropping were significantly improved compared to their monoculture,but no such effects were observed in the plants of YC03-3/maize intercropping.The yield of BD2 intercropped with maize exhibited a 37.5%increment above that of BD2 in monoculture.Moreover,19.2-29.1%longer root length of maize and 19.0-39.4%larger root volume of BD2 were observed in BD2/maize intercropping than in monoculture,but no growth advantage was observed in YC03-3/maize intercropping.Maize showed root avoidance when intercropped with BD2,but space competition when intercropped with YC03-3.16S rRNA amplicon sequencing showed that compared with the monoculture system,rhizobacteria community composition in BD2/maize intercropping changed more significantly than that of the YC03-3/maize intercropping system.In BD2/maize intercropping,most of the rhizobacteria community biomarker bacteria of BD2 were positively correlated with plant biomass,as well as plant P and N content.Maize tended to recruit Rhizobiales and Proteobacteria,which showed positive correlation with plant biomass and N content,respectively,as well as soil available N.In conclusion,soybean varieties determined the advantages of maize/soybean intercropping through root-root interactions and modification of rhizobacteria communities.Our insight emphasizes a linkage between root traits and the rhizobacteria community,which shows the importance of optimizing intercropping systems by selection of appropriate crop varieties.展开更多
A field experiment was conducted to investigate the effect of integrated nano-fertilizer spray and vermicompost in growth and quality of corn yield in calcareous soil at College ofAgricultural Engineering Sciences/Uni...A field experiment was conducted to investigate the effect of integrated nano-fertilizer spray and vermicompost in growth and quality of corn yield in calcareous soil at College ofAgricultural Engineering Sciences/University of Baghdad/Iraq during the autumn season of 2022 using Split Split Plot Design in a randomized complete block design with three replicates.Main plots were designated to two types of vermicompost(local and imported).The secondary plots were assigned to vermicompost added to the soil at three levels of 0,10 and 20 Mg ha-1.The third plots were assigned to nano-fertilizer containing N,P,K,Zn,Fe and Mn at 0,50,100 and 150 mg L-1 sprayed on plant at 20,40 and 60 days after planting.The results showed a significant effect of local vermicompost achieving available nitrogen and phosphorus in the soil as 43.31 mg N kg-1 soil and 19.25 mg P kg-1 soil.In the leaves,nitrogen was 3.86%,potassium 4.54%,iron 174.22 mg Fe kg-1 and Zinc was 73.53 mg Zn kg-1,grain yield was 165.48 Mg ha-1.Adding vermicompost to the soil at 20 Mg ha-1 achieving in available nitrogen in the soil of 43.74 mg N kg-1 soil,available phosphorus of 19.72 mg P kg-1 soil and available potassium of 196.93 mg K kg-1 soil.In the leaves,nitrogen was 4.37%,phosphorus was 0.44%,potassium 4.87%,iron was 182.63 mg Fe kg-1,zinc was 73.70 mg Zn kg-1 and grain yield was 168.43 Mg ha-1.Spraying nano-fertilizer on the plant at 150 mg L-1 achieved In the leaves,nitrogen 4.28%,phosphorus of 0.46%,potassium of 5.10%,iron of 204.83 mg Fe kg-1,zinc of 89.28 mg Zn kg-1 and manganese of 234.07 mg Mn kg-1 and grain yield was 172.88 Mg ha-1.展开更多
Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and t...Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.展开更多
As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production an...As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.展开更多
Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chl...Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chlorophyll accumulation,lower net photosynthesis rate,and fewer grain number per plant than the wild type.To identify genes associated with chlorophyll content,we performed a largescale linkage mapping study using recombinant inbred lines from a cross between the yl mutant and a green-leaf cultivar across three environments.Using quantitative trait locus sequencing(QTL-seq)analysis,we mapped 12 QTL to chromosomes 5,13,15,19,and 20.Of these QTL,one new dominant locus with the largest LOD,named qCC1,was identified consistently and explained 31.73% of the total phenotypic variation on average.Notably,qCC1 was also associated with yield-related traits,including plant height and pod number per plant.Fine-mapping narrowed down qCC1 to an 82.29-kb region.Within this interval,we identified Glyma.15 g087500.1,encoding an ankyrin repeat-containing protein,as the most likely candidate gene,because its homologs are reported to function in thylakoid membrane biogenesis during plastid development.Phenotypic analysis of near-isogenic lines(NILs)revealed that those harboring the qCC1 allele conferring green leaves displayed significantly enhanced chlorophyll content by 136.53%-323.92%,net photosynthesis rate by 11.64%-42.13%,and yield by 111.32% compared with NILs carrying the allele conferring yellow leaves.Comparative transcriptome profiling of NILs coupled with RT-qPCR validation demonstrated that qCC1 up-regulated one differentially expressed gene(DEG)associated with chlorophyll biosynthesis and six DEGs related to photosystem,whereas it downregulated one gene involved in chlorophyll degradation.These findings provide valuable insights into the biological function and regulatory mechanism of chlorophyll metabolism and offer guidance for breeding soybean varieties with enhanced photosynthetic efficiency and high yield.展开更多
Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes ...Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes through gut microbiota modulation.During fermentation,microbial activity alters the starch matrix,enhancing the production of bioactive compounds such as resistant starch,isomalto-oligosaccharides,and resistant dextrin,which improve insulin sensitivity,reduce inflammation,and support glycemic control.Additionally,fermented corn starch harbors beneficial microbial strains including Lactiplantibacillus fermentum,Bifidobacterium breve,and Saccharomyces cerevisiae,which reinforce gut barrier integrity,stimulate incretin secretion,and suppress systemic inflammation.Postbiotic metabolites such as short-chain fatty acids,exopolysaccharides,and bacteriocins further contribute to glucose homeostasis through immune modulation and gut hormone regulation.Despite its promise,the clinical translation of fermented corn starch is limited by safety concerns(e.g.,contamination with pathogens or mycotoxins),lack of standardized fermentation protocols,and a scarcity of targeted studies.This review synthesizes current evidence on the antidiabetic potential of fermented corn starch,advocating for its integration into precision nutrition approaches and supporting further research to address safety and standardization challenges in functional food development.展开更多
Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes h...Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.展开更多
The objective of this study was to characterize the rumination time in lactating dairy cows fed with corn silage.Rumination time was recorded 24 h/day using direct visual observation.Six trials were conducted during 2...The objective of this study was to characterize the rumination time in lactating dairy cows fed with corn silage.Rumination time was recorded 24 h/day using direct visual observation.Six trials were conducted during 2018,2019 and 2020,and rumination time was recorded in 480-2-hour periods from 40 Holstein Friesian cows.In each trial,6 or 8 cows were selected and balanced for days in milk(DIM),milk production and number of lactations.Each cow was recorded continuously for periods of 2 h at a time to complete a full 24-h period per week(12 values per day).Data from all cows were associated with 4 reproductive statuses of cows:Inseminated(1-45 days after insemination),Open(45-150 days after calving),not-pregnant and pregnant.The longest rumination time(RT)was found in pregnant cows(average 536.9±29.87 min/day),and the shortest RT was in open cows(average 420.3±63.2 min/day).Inseminated and non-pregnant cows were found with intermediate values(527.3±82.4 min/day and 467.1±30.7 respectively).Significantly different RT means were found between pregnant and open cows(p<0.0001),pregnant vs.inseminated cows(p<0.0001),and between inseminated and open cows(p=0.0005).We concluded that some gynecological conditions of lactating cows affect the RT.Measurement of RT by visual observations proved to be acceptable for the conditions of this study when cows were housed indoors and were fed with partial mixed ration(PMR)based on corn silage.展开更多
Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in So...Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.展开更多
The aim of this research is to assess the energy potential of cow dung and corn cob inputs,with a view to estimating their biogas potential.The inputs were characterized in the microbiology laboratory of the National ...The aim of this research is to assess the energy potential of cow dung and corn cob inputs,with a view to estimating their biogas potential.The inputs were characterized in the microbiology laboratory of the National Control Office Quality in Matoto,Conakry.The experiment to produce methane from cow dung and maize cob was carried out at the Applied Research in Natural Sciences Laboratory of the University of Kindia(UK)using the following equipment:Three digesters were each connected to an air chamber(gasometer)by means of flexible pipes 8 mm in diameter,connected by clamps,liquid adhesives,valves and Teflon.This research focused on determining the quantity of biogas contained in each type of substrate(cow dung,maize cob and their mixture).Three experiments on the methanization of these inputs were carried out,with anaerobic digestion lasting 27 days,in a temperature range of 27℃ to 31℃(mesophilic range).The results were as follows:maize cob 28.4 L,cow dung 22.6 L and codigestion 38.7 L.These results compared with similar studies revealed a coincidence.展开更多
基金Supported by Double-establishment of Chengdu Cereals and Commercial Crop Industries in 2014~~
文摘The research mainly analyzed effects of waxy corn/soybean intercropping on yields of the two crops, as well as agronomic characters, and the economic benefits of the mode. The results showed that although yields of waxy corn and soybean went down by 21.19% and 31.04% per unit area, land equivalent ratio（1.48） kept higher than 1, suggesting the intercropping improves land use rate. Besides, due to the practice of intercropping, many characters of waxy corn grew, but of soybean declined. The economic benefits from high to low were waxy corn/soybean intercropping, monoculture of waxy corn, and monoculture of soybean.
文摘[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercropping fields in Siyang County.[Methods]The trial comprised six herbicide treatments and one blank control,with investigations conducted to assess efficacy,safety,and yield.[Results]Each herbicide treatment effectively controlled weeds,demonstrated high safety,and enhanced the yields of both soybeans and corn.The combined application of soil sealing with stem and leaf spray exhibited superior overall weed control compared to soil sealing alone.At 28 d following stem and leaf spray,the plant control effect and fresh weight control effect against weeds in the combined treatment of soil sealing with stem and leaf spray all exceeded 89%.[Conclusions]This study offers technical support for advancing the practice of strip intercropping between corn and soybeans.
基金Supported by Special Project for the Construction of the National Modern Agricultural Industry Technology System(CARS-04-CES16).
文摘The soybean and corn strip compound planting technology is a crucial measure for improving land use efficiency and ensuring food security.This paper deeply analyzed the principles,advantages,and key technical aspects of this technology,including variety selection,planting pattern,sowing management,and field management.It also illustrated its application effectiveness through practical cases and proposed corresponding solutions to existing challenges in its promotion.This study provides theoretical support and practical reference for the widespread adoption and efficient application of this technology.
文摘Struvite (MgNH<sub>4</sub>PO<sub>4</sub>·6H<sub>2</sub>O) produced synthetically from a stock solution of known phosphorus (P) and nitrogen (N) concentrations has been shown to be an effective, alternative fertilizer-P source for various crops, but little is known about the potential agronomic effectiveness of struvite created from an actual municipal wastewater source. The objective of this study was to evaluate the effects of soil [i.e., Creldon silt loam (Oxyaquic Fragiudalf) and Calloway silt loam (Aquic Fraglossudalf) series], fertilizer-P source [i.e., synthetically produced electrochemically precipitated struvite (ECSTsyn), real-wastewater-derived ECST (ECSTreal), chemically precipitated struvite (CPST), monoammonium phosphate (MAP), and an unamended control (UC)], and irrigation water type (i.e., tapwater and struvite-removed wastewater) on corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] growth and N, P, and magnesium (Mg) uptake in a 60-day, greenhouse potted-plant study. Crop growth and N, P, and Mg uptakes for the struvite treatments (i.e., CPST, ECSTsyn, and ECSTreal) were generally similar to or at least 1.2 times greater than MAP. The ECSTsyn material commonly had up to five times greater N, P, and Mg uptake in corn and soybean than any other fertilizer-P source. Struvite-removed wastewater resulted in at least 1.3 times lower dry matter and N, P, and Mg uptake than tapwater. Similar corn and soybean results from the struvite fertilizers among the various soil-water type combinations compared to MAP suggest that struvite generates similar crop responses as at least one widely used, commercially available, multi-nutrient fertilizer-P source.
基金supported by the Fundamental Research Funds for the Central Universities(QNTD202302)National Natural Science Foundation of China(22378024)the Foreign expert program(G2022109001L).
文摘Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin first”strategy,corn straw is converted to valuable chemicals including lignin monomers,furfural and 5-methoxymethylfurfural via a two steps process.The key of this research lies in the development of a green and low-cost catalytic process utilizing magnetic Raney Ni catalyst and high boiling point ethylene glycol.The utilization of neat ethylene glycol as the sole slovent under atmospheric conditions obviates the need for additional additives,thereby facilitating the entire process to be conducted in glass flasks and rendering it highly convenient for scaling up.In the initial step,depolymerization of corn straw lignin resulted in a monomer yield of 18.1 wt%.Subsequently,in a dimethyl carbonate system,the carbohydrate component underwent complete conversion in a one-pot process,yielding furfural and 5-methoxymethylfurfural as the primary products with an impressive yield of 47.7%.
基金The financial support from the National Pork Board,Des Moines,IA,USA,is greatly appreciated。
文摘Background Reduction of the particle size of corn increases energy digestibility and concentrations of digestible and metabolizable energy.Pelleting may also reduce particle size of grain,but it is not known if there are interactions between particle size reduction and pelleting.The objective of this experiment was to test the hypothesis that particle size reduction and pelleting,separately or in combination,increase N balance,apparent total tract digestibility(ATTD)of fiber and fat,and net energy(NE)in corn-soybean meal diets fed to group-housed pigs.Methods Six corn-soybean meal-based diets were used in a 3×2 factorial design with 3 particle sizes of corn(i.e.,700,500,or 300μm)and 2 diet forms(i.e.,meal or pelleted).Pigs were allowed ad libitum access to feed and water.Twenty-four castrated male pigs(initial weight:29.52 kg;standard diviation:1.40)were allotted to the 6 diets using a 6×6 Latin square design with 6 calorimeter chambers(i.e.,4 pigs/chamber)and 6 periods.Oxygen consumption and CO_(2)and CH_(4)productions were measured during fed and fasting states and fecal and urine samples were collected.Results Regardless of particle size of corn,the ATTD of gross energy(GE),N,and acid-hydrolyzed ether extract(AEE),and the concentration of NE were greater(P<0.05)in pelleted diets than in meal diets.Regardless of diet form,the ATTD of GE,N,and AEE,and the concentration of NE were increased(linear;P<0.05)by reducing the particle size of corn,but the increase was greater in meal diets than in pelleted diets(interaction;P<0.05).Conclusions Both pelleting and reduction of corn particle size increased nutrient digestibility and NE,but increases were greater in meal diets than in pelleted diets.
文摘To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.
基金supported by the Biological Breeding-National Science and Technology Major Project(2023ZD0403305)National Natural Science Foundation of China(32101845)+1 种基金the National Key Research and Development Program of China(2023YFE0105000)the China Agriculture Research System(CARS-04).
文摘Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop morphological development and increase yield.Here,a three-year study was performed to verify the feasibility of adjusting row spacing to further enhance yield in densely planted soybeans.Of three row-spacing configurations(40-40,20-40,and 20-60 cm)and two planting densities(normal 180,000 plants ha 1 and high 270,000 plants ha 1).The differences in canopy structure,plant morphological development,photosynthetic capacity and their impact on yield were analyzed.Row spacing configurations have a significant effect on canopy transmittance(CT).The 20-60 cm row spacing configuration increased CT and creates a favorable canopy light environment,in which plant height is reduced,while branching is promoted.This approach reduces plant competition,optimizes the developments of leaf area per plant,specific leaf area,leaf area development rate,leaf area duration and photosynthetic physiological indices(F_(v)/F_(m),ETR,P_(n)).The significant increase of 11.9%-34.2%in canopy apparent photosynthesis(CAP)is attributed to the significant optimization of plant growth and photosynthetic physiology through CT,an important contributing factor to yield increases.The yield in the 20-60 cm treatment is 4.0%higher than in equidistant planting under normal planting density,but 5.9%under high density,primarily driven by CAP and pod number.These findings suggest that suitable row spacing configurations optimize the light environment for plants,promote source-sink transformation in soybeans,and further improve yield.In practice,a 20-60 cm row spacing configuration could be employed for high-density soybean planting to achieve a more substantial yield gain.
文摘A flower-like BiOBr photocatalyst(CS/BiOBr)was prepared by using the carbon material derived from corn straw(CS)as the carrier.The prepared composites were characterized by X-ray diffraction(XRD),Fourier transform infrared(FIIR)spectra,scanning electron microscope(SEM),X-ray photoelectron spectra(XPS),and UV-Vis diffuse reflectance spectra(UV-Vis DRS).The SEM analyses indicate that the introduction of CS promotes the formation of a unique flower-like structure in BiOBr,which not only optimizes the efficiency of light capture but also increases the specific surface area of BiOBr.The bandgap of the composite was narrower compared with the pure BiOBr.The CS/BiOBr composites exhibited higher photocatalytic activity than pure CS and BiOBr under visible light irradiation,and a higher first-order reaction rate constant(k)of 0.0437 min-1 than BiOBr(0.0146 min^(-1)),and exhibited excellent stability and reusability during the cyclic run.The enhanced photocatalytic activity is attributed to the efficient separation of photoinduced electrons and holes.Superoxide radicals and holes were the major active species.
基金supported by the Ten-thousand Talents Plan of Zhejiang Province(No.2022R52021)the Key Research and Development Program of Zhejiang Province(No.2021C04016)the Pioneer and Leading Goose R&D Program of Zhejiang(No.2022C04020),China.
文摘Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value.Despite these benefits,SBM contains various antigen proteins such as glycinin andβ-conglycinin,which account for approximately 70%of the total proteins of the SBM and reduce digestibility and damage intestinal function(Peng et al.,2018).
基金supported by the National Key Research and Development Program of China(2021YFF1000504 and 2023YFD1901300)the National Natural Science Foundation of China(32172658,32172659 and 32302662)the Natural Science Foundation of Guangdong Province,China(2021A1515010826).
文摘Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is intercropped with different soybean varieties.In this study,field experiments were conducted to compare the growth and yield of two soybean(Glycine max)varieties,BD2 and YC03-3,and one maize(Zea mays)variety,Huazhen,in mono-cropped and intercropped cultures.The plant biomass and N content of both crops in BD2/maize intercropping were significantly improved compared to their monoculture,but no such effects were observed in the plants of YC03-3/maize intercropping.The yield of BD2 intercropped with maize exhibited a 37.5%increment above that of BD2 in monoculture.Moreover,19.2-29.1%longer root length of maize and 19.0-39.4%larger root volume of BD2 were observed in BD2/maize intercropping than in monoculture,but no growth advantage was observed in YC03-3/maize intercropping.Maize showed root avoidance when intercropped with BD2,but space competition when intercropped with YC03-3.16S rRNA amplicon sequencing showed that compared with the monoculture system,rhizobacteria community composition in BD2/maize intercropping changed more significantly than that of the YC03-3/maize intercropping system.In BD2/maize intercropping,most of the rhizobacteria community biomarker bacteria of BD2 were positively correlated with plant biomass,as well as plant P and N content.Maize tended to recruit Rhizobiales and Proteobacteria,which showed positive correlation with plant biomass and N content,respectively,as well as soil available N.In conclusion,soybean varieties determined the advantages of maize/soybean intercropping through root-root interactions and modification of rhizobacteria communities.Our insight emphasizes a linkage between root traits and the rhizobacteria community,which shows the importance of optimizing intercropping systems by selection of appropriate crop varieties.
文摘A field experiment was conducted to investigate the effect of integrated nano-fertilizer spray and vermicompost in growth and quality of corn yield in calcareous soil at College ofAgricultural Engineering Sciences/University of Baghdad/Iraq during the autumn season of 2022 using Split Split Plot Design in a randomized complete block design with three replicates.Main plots were designated to two types of vermicompost(local and imported).The secondary plots were assigned to vermicompost added to the soil at three levels of 0,10 and 20 Mg ha-1.The third plots were assigned to nano-fertilizer containing N,P,K,Zn,Fe and Mn at 0,50,100 and 150 mg L-1 sprayed on plant at 20,40 and 60 days after planting.The results showed a significant effect of local vermicompost achieving available nitrogen and phosphorus in the soil as 43.31 mg N kg-1 soil and 19.25 mg P kg-1 soil.In the leaves,nitrogen was 3.86%,potassium 4.54%,iron 174.22 mg Fe kg-1 and Zinc was 73.53 mg Zn kg-1,grain yield was 165.48 Mg ha-1.Adding vermicompost to the soil at 20 Mg ha-1 achieving in available nitrogen in the soil of 43.74 mg N kg-1 soil,available phosphorus of 19.72 mg P kg-1 soil and available potassium of 196.93 mg K kg-1 soil.In the leaves,nitrogen was 4.37%,phosphorus was 0.44%,potassium 4.87%,iron was 182.63 mg Fe kg-1,zinc was 73.70 mg Zn kg-1 and grain yield was 168.43 Mg ha-1.Spraying nano-fertilizer on the plant at 150 mg L-1 achieved In the leaves,nitrogen 4.28%,phosphorus of 0.46%,potassium of 5.10%,iron of 204.83 mg Fe kg-1,zinc of 89.28 mg Zn kg-1 and manganese of 234.07 mg Mn kg-1 and grain yield was 172.88 Mg ha-1.
基金supported by the National Key Research and Development Program of China(2022YFF1001502)Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)Major Projects of Technological Innovation 2030(2023ZD04037).
文摘Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.
基金Supported by Special Fund for National Modern Agricultural Industry Technology System Construction(CARS-04-CES16).
文摘As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.
基金supported by the Heilongjiang Provincial Natural Science Foundation of China(SS2023C002)。
文摘Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chlorophyll accumulation,lower net photosynthesis rate,and fewer grain number per plant than the wild type.To identify genes associated with chlorophyll content,we performed a largescale linkage mapping study using recombinant inbred lines from a cross between the yl mutant and a green-leaf cultivar across three environments.Using quantitative trait locus sequencing(QTL-seq)analysis,we mapped 12 QTL to chromosomes 5,13,15,19,and 20.Of these QTL,one new dominant locus with the largest LOD,named qCC1,was identified consistently and explained 31.73% of the total phenotypic variation on average.Notably,qCC1 was also associated with yield-related traits,including plant height and pod number per plant.Fine-mapping narrowed down qCC1 to an 82.29-kb region.Within this interval,we identified Glyma.15 g087500.1,encoding an ankyrin repeat-containing protein,as the most likely candidate gene,because its homologs are reported to function in thylakoid membrane biogenesis during plastid development.Phenotypic analysis of near-isogenic lines(NILs)revealed that those harboring the qCC1 allele conferring green leaves displayed significantly enhanced chlorophyll content by 136.53%-323.92%,net photosynthesis rate by 11.64%-42.13%,and yield by 111.32% compared with NILs carrying the allele conferring yellow leaves.Comparative transcriptome profiling of NILs coupled with RT-qPCR validation demonstrated that qCC1 up-regulated one differentially expressed gene(DEG)associated with chlorophyll biosynthesis and six DEGs related to photosystem,whereas it downregulated one gene involved in chlorophyll degradation.These findings provide valuable insights into the biological function and regulatory mechanism of chlorophyll metabolism and offer guidance for breeding soybean varieties with enhanced photosynthetic efficiency and high yield.
基金Supported by the Research Office,University of KwaZulu-Natal,Durbanan Incentive Grant from the National Research Foundation,Pretoria,South Africa,No.145943.
文摘Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes through gut microbiota modulation.During fermentation,microbial activity alters the starch matrix,enhancing the production of bioactive compounds such as resistant starch,isomalto-oligosaccharides,and resistant dextrin,which improve insulin sensitivity,reduce inflammation,and support glycemic control.Additionally,fermented corn starch harbors beneficial microbial strains including Lactiplantibacillus fermentum,Bifidobacterium breve,and Saccharomyces cerevisiae,which reinforce gut barrier integrity,stimulate incretin secretion,and suppress systemic inflammation.Postbiotic metabolites such as short-chain fatty acids,exopolysaccharides,and bacteriocins further contribute to glucose homeostasis through immune modulation and gut hormone regulation.Despite its promise,the clinical translation of fermented corn starch is limited by safety concerns(e.g.,contamination with pathogens or mycotoxins),lack of standardized fermentation protocols,and a scarcity of targeted studies.This review synthesizes current evidence on the antidiabetic potential of fermented corn starch,advocating for its integration into precision nutrition approaches and supporting further research to address safety and standardization challenges in functional food development.
基金supported by the National Key R&D Program of China(2022YFD1201802)the Shandong Key R&D Program,China(2022CXGC010607)+2 种基金the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202109)the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2017-ICS)the Subproject of the Major Project of Science and Technology in Shanxi Province,China(202201140601025-1-02)。
文摘Southern corn rust(SCR)is an airborne fungal disease caused by Puccinia polysora Underw.(P.polysora)that adversely impacts maize quality and yields worldwide.Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.Using phenotypic SCR resistance-related data collected over two years and three geographical environments,a genome-wide association study was carried out in this work,which eventually identified 91 loci that were substantially correlated with SCR susceptibility.These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes(B73_RefGen_v4).Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection,with 2,586 and 797 differentially expressed genes(DEGs)ultimately being identified in the resistant Qi319and susceptible 8112 inbred lines following P.polysora infection,respectively,including 306 genes common to both lines.Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.One of these genes is ZmHCT9,which encodes the protein hydroxycinnamoyl transferase 9.This gene was up-regulated in susceptible inbred lines and linked to greater P.polysora resistance as confirmed through cucumber mosaic virus(CMV)-based virus induced-gene silencing(VIGS)system-mediated gene silencing.These data provide important insights into the genetic basis of the maize SCR response.They will be useful for for future research on potential genes related to SCR resistance in maize.
文摘The objective of this study was to characterize the rumination time in lactating dairy cows fed with corn silage.Rumination time was recorded 24 h/day using direct visual observation.Six trials were conducted during 2018,2019 and 2020,and rumination time was recorded in 480-2-hour periods from 40 Holstein Friesian cows.In each trial,6 or 8 cows were selected and balanced for days in milk(DIM),milk production and number of lactations.Each cow was recorded continuously for periods of 2 h at a time to complete a full 24-h period per week(12 values per day).Data from all cows were associated with 4 reproductive statuses of cows:Inseminated(1-45 days after insemination),Open(45-150 days after calving),not-pregnant and pregnant.The longest rumination time(RT)was found in pregnant cows(average 536.9±29.87 min/day),and the shortest RT was in open cows(average 420.3±63.2 min/day).Inseminated and non-pregnant cows were found with intermediate values(527.3±82.4 min/day and 467.1±30.7 respectively).Significantly different RT means were found between pregnant and open cows(p<0.0001),pregnant vs.inseminated cows(p<0.0001),and between inseminated and open cows(p=0.0005).We concluded that some gynecological conditions of lactating cows affect the RT.Measurement of RT by visual observations proved to be acceptable for the conditions of this study when cows were housed indoors and were fed with partial mixed ration(PMR)based on corn silage.
基金supported by National Key R&D Program of China(2022YFF1100300)Joint Fund of Henan Province Science and Technology R&D Program(235200810051)+1 种基金Key Project in Science and Technology Agency of Henan Province(242102310561)key research projects of higher education institutions in Henan Province(24B350002).
文摘Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.
文摘The aim of this research is to assess the energy potential of cow dung and corn cob inputs,with a view to estimating their biogas potential.The inputs were characterized in the microbiology laboratory of the National Control Office Quality in Matoto,Conakry.The experiment to produce methane from cow dung and maize cob was carried out at the Applied Research in Natural Sciences Laboratory of the University of Kindia(UK)using the following equipment:Three digesters were each connected to an air chamber(gasometer)by means of flexible pipes 8 mm in diameter,connected by clamps,liquid adhesives,valves and Teflon.This research focused on determining the quantity of biogas contained in each type of substrate(cow dung,maize cob and their mixture).Three experiments on the methanization of these inputs were carried out,with anaerobic digestion lasting 27 days,in a temperature range of 27℃ to 31℃(mesophilic range).The results were as follows:maize cob 28.4 L,cow dung 22.6 L and codigestion 38.7 L.These results compared with similar studies revealed a coincidence.
