Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to p...Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found.展开更多
Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing p...Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.展开更多
Background:Onion grass(Romulea rosea)is a common weed that infests native and improved pastures in the Mediterranean environments of southern Australia.It is a very challenging weed to control due to its distinctive g...Background:Onion grass(Romulea rosea)is a common weed that infests native and improved pastures in the Mediterranean environments of southern Australia.It is a very challenging weed to control due to its distinctive growth and survival mechanisms involving corms.Methods:Three glasshouse experiments were conducted in Australia to investigate the response of onion grass to defoliation and fertiliser application,the development and growth of onion grass corms and the chemical control of the weed in winter and spring.Results:Defoliating consistently to 1 cm above ground reduced corm weight by 84%compared with the control.Medium or high fertiliser application did not increase herbage mass of onion grass compared with the nonfertilised treatment.The corm weight of onion grass declined from Weeks 1 to 8 after emergence,remained at 53-60 mg dry matter(DM)corm−1 from Weeks 8 to 19 and then declined on average to 37mgDMcorm−1 up to Week 26 after emergence.New corms started to develop at Week 6 after emergence.A small proportion of the onion grass plants(7.5%)developed multiple new corms(3-6 corms)from a single old corm.The most effective herbicide control was a winter application of metsulfuron methyl or imazamox as an alternative product to protect clover.Conclusions:This study has identified the growth and development patterns of onion grass corms,how the plant responded to defoliation and fertiliser application and effective chemical control of this weed.These findings have significant practical implications for the improvement of onion grass-infested pastures.展开更多
利用^(15)N同位素示踪和转录组测序,系统地研究了花魔芋(Amorphophallus konjac K. Koch)球茎发育过程中各器官氮素分配特征,以及氮代谢相关基因表达情况,以理解氮素利用情况并指导合理用肥。结果表明,在球茎发育过程中,叶片的含氮量最...利用^(15)N同位素示踪和转录组测序,系统地研究了花魔芋(Amorphophallus konjac K. Koch)球茎发育过程中各器官氮素分配特征,以及氮代谢相关基因表达情况,以理解氮素利用情况并指导合理用肥。结果表明,在球茎发育过程中,叶片的含氮量最高;随球茎膨大,叶片的含氮量、Ndff值、^(15)N分配率和利用率呈下降趋势,而球茎对氮素的需求增加,^(15)N利用率高峰出现在球茎膨大中后期(散叶后45~75 d)。整个植株^(15)N利用率呈现先增加后降低的趋势,在散叶后60 d时整个植株中的N含量达到峰值,^(15)N利用率最高,为22.68%,之后在各个器官中N素含量与^(15)N利用率均逐步降低,说明在60d时是氮素的最大利用效率期,从此开始植物吸收的氮素由主要分配给叶片转向分配给球茎,此时为追施氮肥的适宜期。叶片中氮代谢相关基因表达高峰出现在球茎膨大中后期(散叶后105d),此时叶片的氮代谢反应加强,有利于氮同化产物合成和输出。叶片中硝酸还原酶基因AkNIA1和谷氨酰胺合成酶基因AkGSR2-1表达水平与叶片和根中N含量显著正相关,表明其在促进氮吸收和氮积累中的重要作用,可作为氮利用率遗传改良的候选基因。展开更多
Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stre...Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stress, the biomass allocations to different plant components and some related morphological parameters were examined along an elevational gradient within a salt marsh. Authors found that S. mariqueter performed best at medium elevation within the marsh, with relatively high density of shoot and individual ramet dry mass. Biomass allocation to corm was the highest at low elevations, and the least at high elevations, suggesting that a conservative strategy was adopted by the species to cope with the harsh physical conditions at the low elevation. The investment in rhizome decreased from low to high elevations, while the proportion of inflorescence mass increased, indicating that during the life history, the species shifts from predominant asexual reproduction to predominant sexual reproduction. This may be favourable for the species to colonize larger area, and to spread and persist at a meta_population level. Correlation analyses showed that sexual reproduction was inversely related to growth and asexual reproduction. However, it is difficult to determine the relationship between asexual reproduction and growth possibly because of the varied function of the corms of the species in different life history stages.展开更多
Comparative morphological study of five varieties of Colocasia esculenta (L.) Schott present in Anambra State, Nigeria was carried out, in order to furnish plant taxonomists with information which could be of great he...Comparative morphological study of five varieties of Colocasia esculenta (L.) Schott present in Anambra State, Nigeria was carried out, in order to furnish plant taxonomists with information which could be of great help in delimitation of the varieties. Significant difference was established at p < 0.05. The result showed that adaxial surface of “kochuo” had a purplish dot on the centre, abaxial leaf surface had a conspicuous purplish colour at the point of attachment to the leaf, and the cormels had numerous striking vertical purple stripes on the surface. There was presence of foliaceous (leaf-like) appendages at the veins of the abaxial surface of the leaf of “ogeriobosi”. The leaf length ranged from 35.6 ± 7.70 cm (“kochuo”) to 49.9 ± 3.55 cm (“ogeriobosi”). Petiole length of “ogeriobosi” was the highest (63.3 ± 3.83 cm), whereas the least was Colocasia esculenta var. antiquorum (26.67 ± 2.20 cm). The corm length ranged from 4.10 ± 0.10 cm (Colocasia esculenta var. antiquorum) to 8.60 ± 0.35 cm (“ogeriobosi”), while the cormel length ranged from 3.70 ± 0.96 cm (Colocasia esculenta var. antiquorum) to 7.03 ± 0.36 cm (“ogeriobosi”). This work has revealed diagnostic and differential morphological characters, which could be useful for identification and description of varieties of C. esculenta. In addition, it provided additional information which might be helpful in resolving the on-going controversy in the taxonomy of Colocasia, which would, in turn, probably lead to possible delimitation of C. esculenta.展开更多
基金Item supported by science and technologycommittee of Shanghai municipality(003113010)
文摘Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found.
文摘Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals;therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R<sub>0</sub> - 1);2) when the plants shed (R<sub>0</sub>) and 3) at two weeks after the plants shed (R<sub>0</sub> + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.
基金Department of Jobs,Precincts and Regions and the Glenelg-Hopkins Catchment Management,Victoria,Australia.
文摘Background:Onion grass(Romulea rosea)is a common weed that infests native and improved pastures in the Mediterranean environments of southern Australia.It is a very challenging weed to control due to its distinctive growth and survival mechanisms involving corms.Methods:Three glasshouse experiments were conducted in Australia to investigate the response of onion grass to defoliation and fertiliser application,the development and growth of onion grass corms and the chemical control of the weed in winter and spring.Results:Defoliating consistently to 1 cm above ground reduced corm weight by 84%compared with the control.Medium or high fertiliser application did not increase herbage mass of onion grass compared with the nonfertilised treatment.The corm weight of onion grass declined from Weeks 1 to 8 after emergence,remained at 53-60 mg dry matter(DM)corm−1 from Weeks 8 to 19 and then declined on average to 37mgDMcorm−1 up to Week 26 after emergence.New corms started to develop at Week 6 after emergence.A small proportion of the onion grass plants(7.5%)developed multiple new corms(3-6 corms)from a single old corm.The most effective herbicide control was a winter application of metsulfuron methyl or imazamox as an alternative product to protect clover.Conclusions:This study has identified the growth and development patterns of onion grass corms,how the plant responded to defoliation and fertiliser application and effective chemical control of this weed.These findings have significant practical implications for the improvement of onion grass-infested pastures.
文摘Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stress, the biomass allocations to different plant components and some related morphological parameters were examined along an elevational gradient within a salt marsh. Authors found that S. mariqueter performed best at medium elevation within the marsh, with relatively high density of shoot and individual ramet dry mass. Biomass allocation to corm was the highest at low elevations, and the least at high elevations, suggesting that a conservative strategy was adopted by the species to cope with the harsh physical conditions at the low elevation. The investment in rhizome decreased from low to high elevations, while the proportion of inflorescence mass increased, indicating that during the life history, the species shifts from predominant asexual reproduction to predominant sexual reproduction. This may be favourable for the species to colonize larger area, and to spread and persist at a meta_population level. Correlation analyses showed that sexual reproduction was inversely related to growth and asexual reproduction. However, it is difficult to determine the relationship between asexual reproduction and growth possibly because of the varied function of the corms of the species in different life history stages.
文摘Comparative morphological study of five varieties of Colocasia esculenta (L.) Schott present in Anambra State, Nigeria was carried out, in order to furnish plant taxonomists with information which could be of great help in delimitation of the varieties. Significant difference was established at p < 0.05. The result showed that adaxial surface of “kochuo” had a purplish dot on the centre, abaxial leaf surface had a conspicuous purplish colour at the point of attachment to the leaf, and the cormels had numerous striking vertical purple stripes on the surface. There was presence of foliaceous (leaf-like) appendages at the veins of the abaxial surface of the leaf of “ogeriobosi”. The leaf length ranged from 35.6 ± 7.70 cm (“kochuo”) to 49.9 ± 3.55 cm (“ogeriobosi”). Petiole length of “ogeriobosi” was the highest (63.3 ± 3.83 cm), whereas the least was Colocasia esculenta var. antiquorum (26.67 ± 2.20 cm). The corm length ranged from 4.10 ± 0.10 cm (Colocasia esculenta var. antiquorum) to 8.60 ± 0.35 cm (“ogeriobosi”), while the cormel length ranged from 3.70 ± 0.96 cm (Colocasia esculenta var. antiquorum) to 7.03 ± 0.36 cm (“ogeriobosi”). This work has revealed diagnostic and differential morphological characters, which could be useful for identification and description of varieties of C. esculenta. In addition, it provided additional information which might be helpful in resolving the on-going controversy in the taxonomy of Colocasia, which would, in turn, probably lead to possible delimitation of C. esculenta.
