A rapid, specific, sensitive, and precise reverse-phase HPLC method for the quantitative determination of process related and degradation impurities of Apixaban, an anticoagulant drug is described. The developed RP-HP...A rapid, specific, sensitive, and precise reverse-phase HPLC method for the quantitative determination of process related and degradation impurities of Apixaban, an anticoagulant drug is described. The developed RP-HPLC method was successfully applied to the analysis of both Apixaban drug substance and drug product. The chromatographic separation was achieved on a Sigma-Aldrich’s Ascentis Express®C18 (4.6 mm × 100 mm, 2.7 μ) HPLC column with a runtime of 40 min. Mobile phase-A and mobile phase-B were phosphate buffer and acetonitrile respectively. The column oven temperature was set at 35°C and photodiode array detector was set at 225 nm. Nine process related impurities (Imp-1 to Imp-9) have been detected in test sample of Apixaban by using newly developed RP-HPLC method. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic and thermal conditions to demonstrate the stability-indicating nature of the developed RP-HPLC method. The developed method was validated as per ICH guideline and found to be specific, precise, sensitive and robust.展开更多
The behavior of Beam-Column Joints in moment resisting frame structures are susceptible to damage caused by seismic effects due to poor performance of the joint.A good number of researches were carried out to understa...The behavior of Beam-Column Joints in moment resisting frame structures are susceptible to damage caused by seismic effects due to poor performance of the joint.A good number of researches were carried out to understand the complex mechanism of RC joints which are considered in seismic design code practices presently adopted.The traditional construction detailing of transverse reinforcement have shown serious joint failure. This paper introduces a new design philosophy involving the use of additional diagonal bars within the joint particularly suitable for low to medium seismic effects in earthquake zones throughout the world.In lieu to this study,ten(10) full-scale interior beam-column specimens were constructed with various additional reinforcement details and configurations as will be discussed in the later.The experiment provided adequate results to proof the idea of additional bars as suitable approach in reinforced concrete structures where earthquake is eminent.While compared with overall cracking observation during the test,the specimen with additional bars (diagonal and straight) had shown few cracks on the column than the ones without.Furthermore,concrete confinement is certainly an important design method as recommended by certain international codes.展开更多
文摘A rapid, specific, sensitive, and precise reverse-phase HPLC method for the quantitative determination of process related and degradation impurities of Apixaban, an anticoagulant drug is described. The developed RP-HPLC method was successfully applied to the analysis of both Apixaban drug substance and drug product. The chromatographic separation was achieved on a Sigma-Aldrich’s Ascentis Express®C18 (4.6 mm × 100 mm, 2.7 μ) HPLC column with a runtime of 40 min. Mobile phase-A and mobile phase-B were phosphate buffer and acetonitrile respectively. The column oven temperature was set at 35°C and photodiode array detector was set at 225 nm. Nine process related impurities (Imp-1 to Imp-9) have been detected in test sample of Apixaban by using newly developed RP-HPLC method. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic and thermal conditions to demonstrate the stability-indicating nature of the developed RP-HPLC method. The developed method was validated as per ICH guideline and found to be specific, precise, sensitive and robust.
基金Research Institute of Structural Engineering and Seismic Disaster Prevention,Tongji University
文摘The behavior of Beam-Column Joints in moment resisting frame structures are susceptible to damage caused by seismic effects due to poor performance of the joint.A good number of researches were carried out to understand the complex mechanism of RC joints which are considered in seismic design code practices presently adopted.The traditional construction detailing of transverse reinforcement have shown serious joint failure. This paper introduces a new design philosophy involving the use of additional diagonal bars within the joint particularly suitable for low to medium seismic effects in earthquake zones throughout the world.In lieu to this study,ten(10) full-scale interior beam-column specimens were constructed with various additional reinforcement details and configurations as will be discussed in the later.The experiment provided adequate results to proof the idea of additional bars as suitable approach in reinforced concrete structures where earthquake is eminent.While compared with overall cracking observation during the test,the specimen with additional bars (diagonal and straight) had shown few cracks on the column than the ones without.Furthermore,concrete confinement is certainly an important design method as recommended by certain international codes.
