To estimate the impact of copying on the indoor air quality, and to investigate whether ozone emitted during such a process induces pathological oxidative stress and potential oxidative damage in the bodies of operato...To estimate the impact of copying on the indoor air quality, and to investigate whether ozone emitted during such a process induces pathological oxidative stress and potential oxidative damage in the bodies of operators. Methods 67 copying operators (CO) and 67 healthy volunteers (HV) were enrolled in a random control study, in which levels of lipoperoxide (LPO) in plasma and erythrocytes, and levels of vitamin C (VC), vitamin E (VE) and b-carotene (b-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined by spectrophotometric methods. Results Compared with the HV group, the average values of LPO in plasma and erythrocytes in the CO group were significantly increased (P<0.0001), while those of VC, VE and b-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the CO group were significantly decreased (P<0.0001). Pearson product-moment correlation analysis showed that with increase of ozone level in copying sites and duration of exposure to ozone, the values of LPO in plasma and erythrocytes in the bodies of operators were gradually increased,while those of VC, VE, b-CAR, SOD, CAT and GPX were decreased in the same manner. Odds ratio (OR) of risk of biochemical parameters reflecting potential oxidative damage of the copying operators ranged from 4.440 to 13.516, and 95 % CI of OR was from 2.113 to 34.061. Reliability coefficient () of the biochemical parameters used to reflect the potential oxidative damage of the operators was 0.8156, standardized item =0.9929, P<0.0001. Conclusion Findings in the present study suggest that there exist a series of free radical chain reactions and pathological oxidative stress induced by high dose ozone in the operators, thereby causing potential oxidative and lipoperoxidative damages in their bodies.展开更多
Mate-choice copying is a fascinating and widespread mate-choice strategy. Individuals gather public information about potential mates by observing others during sexual interactions and choose or reject the same indivi...Mate-choice copying is a fascinating and widespread mate-choice strategy. Individuals gather public information about potential mates by observing others during sexual interactions and choose or reject the same individual as a mate as the observed individual did before. The influence of copying behavior on an individual＇s mate choice can be so strong that socially acquired information can override genetically based preferences for certain phenotypes. Thus, mate-choice copying enforces dy- namic processes in sexual selection. Here, we review the current state of research on mate-choice copying and focus on sex-specific aspects. We present evidence that mate-choice copying can support the evolution of novel sexual ornaments, and we discuss potential costs of mate-choice copying when public information is not reliable. Moreover, we discuss the conflict faced by males that copy since mate-choice copying increases sperm competition. In conclusion we suggest interesting topics for future research in mate-choice copying .展开更多
A criterion is proposed to the feasibility on radial copying grinding, i.e.the pressure angleapplying to a point on the workpiece contour to be profiled should be smaller than its angle limit.Therefore, the expression...A criterion is proposed to the feasibility on radial copying grinding, i.e.the pressure angleapplying to a point on the workpiece contour to be profiled should be smaller than its angle limit.Therefore, the expressions of the angle applying to copying grinding and the angle limit to copyingmechanism are derived, with the measures taken for the quality improvement of copying movementin contour grinding discussed.展开更多
Mate choice copying-when individuals learn to prefer mates or mate types that have been chosen by others-can influence trait evo-lution and speciation(Varela et al.2018;Dion et al.2019).Most examples of mate choice co...Mate choice copying-when individuals learn to prefer mates or mate types that have been chosen by others-can influence trait evo-lution and speciation(Varela et al.2018;Dion et al.2019).Most examples of mate choice copying are from fish,birds,and mammals including humans(Varela et al.2018).However,2 invertebrate examples-fruit flies and wolf spiders-have been used to argue that the phenomenon may be phylogenetically widespread,and perhaps the rule rather than the exception in nature(Varela et al.2018).Here,we revisit the evidence for mate choice copying in wolf spiders(Fowler-Fimn et al.2015)in light of new data(Gilman et al.2018).Then,we discuss why mate choice copying is a phenomenon that is likely to occur in wolf spiders,and why this deserves attention.展开更多
We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubit...We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubitsystem. There may exist inter-three-qubit entanglement and inter-two-qubit entanglement for the three-qubit system.We show that both local and non-local copying processes degrade quantum entanglement in the three-particle systemdue to a residual correlation between the copied output and the copying machine. We also show that the inter-two-qubitentanglement is preserved better than the inter-three-qubit entanglement in the local cloning process. We find thatnon-local cloning is much more efficient than the local copying for broadcasting entanglement, and output state vianon-local cloning exhibits the fidelity better than local cloning.展开更多
We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubit...We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubitsystem. There may exist inter-three-qubit entanglement and inter-two-qubit entanglement for the three-qubit system.We show that both local and non-local copying processes degrade quantum entanglement in the three-particle systemdue to a residual correlation between the copied output and the copying machine. We also show that the inter-two-qubitentanglement is preserved better than the inter-three-qubit entanglement in the local cloning process. We find thatnon-local cloning is much more efficient than the local copying for broadcasting entanglement, and output state vianon-local cloning exhibits the fidelity better than local cloning.展开更多
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,para...Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).展开更多
In order to celebrate its second anniversary,a shopping center in Guiyang,capital of southwest China’s Guizhou Province,organized a tomato fight on July 20.The idea was based on the famed tomato festivalin Spain.
Implementation of robot-based motion control in optical machining demonstrably enhances the machining quality.The introduction of motion-copying method enables learning and replicating manipulation from experienced te...Implementation of robot-based motion control in optical machining demonstrably enhances the machining quality.The introduction of motion-copying method enables learning and replicating manipulation from experienced technicians.Nevertheless,the location uncertainties of objects and frequent switching of manipulated spaces in practical applications impose constraints on their further advancement.To address this issue,a motion-copying system with a symbol-sequence-based phase switch control(SSPSC)scheme was developed by transferring the operating skills and intelligence of technicians to mechanisms.The manipulation process is decomposed,symbolised,rearranged,and reproduced according to the manufacturing characteristics regardless of the change in object location.A force-sensorless adaptive sliding-mode-assisted reaction force observer(ASMARFOB),wherein a novel dual-layer adaptive law was designed for high-performance fine force sensing,was established.The uniformly ultimate boundedness(UUB)of the ASMARFOB is guaranteed based on the Lyapunov stability theory,and the switching stability of the SSPSC was examined.Validation simulations and experiments demonstrated that the proposed method enables better motion reproduction with high consistency and adaptability.The findings of this study can provide effective theoretical and practical guidance for high-precision intelligent optical manufacturing.展开更多
Subtropical evergreen broad-leaved trees are usually vulnerable to freezing stress,while hexaploid wild Camellia oleifera shows strong freezing tolerance.As a valuable genetic resource of woody oil crop C.oleifera,wil...Subtropical evergreen broad-leaved trees are usually vulnerable to freezing stress,while hexaploid wild Camellia oleifera shows strong freezing tolerance.As a valuable genetic resource of woody oil crop C.oleifera,wild C.oleifera can serve as a case for studying the molecular bases of adaptive evolution to freezing stress.Here,47 wild C.oleifera from 11 natural distribution sites in China and 4 relative species of C.oleifera were selected for genome sequencing.“Min Temperature of Coldest Month”(BIO6)had the highest comprehensive contribution to wild C.oleifera distribution.The population genetic structure of wild C.oleifera could be divided into two groups:in cold winter(BIO6≤0℃)and warm winter(BIO6>0℃)areas.Wild C.oleifera in cold winter areas might have experienced stronger selection pressures and population bottlenecks with lower N_(e) than those in warm winter areas.155 singlenucleotide polymorphisms(SNPs)were significantly correlated with the key bioclimatic variables(106 SNPs significantly correlated with BIO6).Twenty key SNPs and 15 key copy number variation regions(CNVRs)were found with genotype differentiation>50%between the two groups of wild C.oleifera.Key SNPs in cis-regulatory elements might affect the expression of key genes associated with freezing tolerance,and they were also found within a CNVR suggesting interactions between them.Some key CNVRs in the exon regions were closely related to the differentially expressed genes under freezing stress.The findings suggest that rich SNPs and CNVRs in polyploid trees may contribute to the adaptive evolution to freezing stress.展开更多
Hua Shou(1304-1386)styled himself Ying Ning Sheng.He was also known as Hua Boren.His ancestral home was in Xiang Town,Xu Prefecture(present Xuchang,Henan province).His birth place was Yizhen,Jiangsu province.Later he ...Hua Shou(1304-1386)styled himself Ying Ning Sheng.He was also known as Hua Boren.His ancestral home was in Xiang Town,Xu Prefecture(present Xuchang,Henan province).His birth place was Yizhen,Jiangsu province.Later he moved to Yuyao,Zhejiang province.He was one of the distinguished practitioners of the late Yuan and early Ming dynasties.His writings include Elaboration of the Fourteen Meridians(Shi Si Jing Fa Hui),Notes on Handwritten Copy of Basic Questions(Du Su Wen Chao),The Essentials for Physicians(Zhen Jia Shu Yao).展开更多
Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise fr...Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise from the brain processing of an efference copy of the motor command in sensory areas.However,recent research suggests that feedback from muscle spindles plays a significant role in this perception.In this study,tendon vibration protocols were employed to attenuate sensory feedback during subsequent cycling exercises.The aim was to assess whether vibration would increase cycling power output,muscle activation,and heart rate at fixed perceived effort intensities.Methods:Fifteen healthy young participants completed 2 experimental visits(vibration and sham).In each visit,participants performed two 3-min cycling bouts,1 at a moderate perceived effort intensity and 1 at a strong perceived effort intensity,before(pre)and after(post)an actual or a sham vibration protocol.Vibration was applied bilaterally on the patellar and Achilles tendons for 10 min.Power output,heart rate,and vastus lateralis electromyography(VL EMG)were recorded and averaged for each bout.Absolute values as well as relative change(%)between pre and post conditions were compared across sham and vibration conditions.Results:At moderate perceived effort,power output,heart rate,and VL EMG increased post-vibration compared to pre-vibration(p<0.05),while no difference was observed in the sham condition.At strong perceived effort,power output and VL EMG decreased post-sham(p<0.05)but remained unchanged post-vibration.Moreover,the relative change between pre and post conditions was significantly higher in the vibration conditions compared to the sham condition for all variables.Conclusion:This study shows that tendon vibration reduces effort perception during subsequent cycling bouts.This effect is likely a consequence of vibration-induced reduction in muscle spindle reafferent signaling to the brain,but this mechanism remains to be further elucidated.From an applied perspective,these findings highlight tendon vibration as a promising tool for enhancing physical activity engagement.展开更多
The mitochondrial DNA copy number(mtDNAcn)plays a vital role in cellular energy metabolism and mitochondrial health.As mitochondria are responsible for adenosine triphosphate production through oxidative phosphorylati...The mitochondrial DNA copy number(mtDNAcn)plays a vital role in cellular energy metabolism and mitochondrial health.As mitochondria are responsible for adenosine triphosphate production through oxidative phosphorylation,maintaining an appropriate mtDNAcn level is vital for the overall cellular function.Alterations in mtDNAcn have been linked to various diseases,including neurodegenerative disorders,metabolic conditions,and cancers,making it an important biomarker for understanding the disease pathogenesis.The accurate estimation of mtDNAcn is essential for clinical applications.Quantitative polymerase chain reaction and next-generation sequencing are commonly employed techniques with distinct advantages and limitations.Clinically,mtDNAcn serves as a valuable indicator for early diagnosis,disease progression,and treatment response.For instance,in oncology,elevated mtDNAcn levels in blood samples are associated with tumor aggressiveness and can aid in monitoring treatment efficacy.In neurodegenerative diseases such as Alzheimer’s and Parkinson’s,altered mtDNAcn patterns provide insights into disease mechanisms and progression.Understanding and estimating mtDNAcn are critical for advancing diagnostic and therapeutic strategies in various medical fields.As research continues to uncover the implications of mtDNAcn alterations,its potential as a clinical biomarker is likely to expand,thereby enhancing our ability to diagnose and manage complex diseases.展开更多
Toxic effects in terms of mitochondria and hereditary substances have been characterized in vitro for individual rare earth elements,while,the joint effects of mixed elements exposure in the population remain ambiguou...Toxic effects in terms of mitochondria and hereditary substances have been characterized in vitro for individual rare earth elements,while,the joint effects of mixed elements exposure in the population remain ambiguous.Based on the Occupational Chromate Exposure Dynamic Cohort of China,this study investigated the relationship between 15 blood rare earth elements(cerium,dysprosium,erbium,europium,gadolinium,holmium,lanthanum,lutetium,neodymium,praseodymium,samarium,terbium,thulium,yttrium,and ytterbium)and mitochondrial DNA copy number(MtDNACN)as well as peripheral blood lymphocyte micronucleus frequency(MNF).The elastic net was used to select elements highly correlated with effect indicators,whose dose-response relationships were further illustrated by restricted cubic splines.Bayesian kernel regressionwas employed to explore the combined effects of elements and the contributions of single element.The results showed that most rare earth elementswere positively correlatedwith effect indicators,with yttrium showing the strongest association(β(95%CI):0.139(0.1089–0.189)for MtDNACN,0.937(0.345–1.684)for MNF).In the mixed exposure model,with the exposure level fixed at the 50th percentile as the reference,the effect estimates on MtDNACN and MNF increased by 0.228 and 0.598 units,respectively,at the 75th percentile.The single effect analysis implied that yttrium,lanthanum and terbium contributed the most to the elevation of MtDNACN,while yttrium posed the highest risk for genetic damage,accordingly,we provided recommendations to prioritize these elements of concern.In addition,we observed a chief mediating effect of MtDNACN on the elevation of MNF caused by lanthanum,whereas further mechanistic exploration is required to confirm this finding.展开更多
In order to isolate the total cDNA of rice (Oryza sativa L.) epsps gene, RT-PCR was carried out with template of rice first-strand cDNA and primers designed according to rice EPSP synthase genomic sequence obtained in...In order to isolate the total cDNA of rice (Oryza sativa L.) epsps gene, RT-PCR was carried out with template of rice first-strand cDNA and primers designed according to rice EPSP synthase genomic sequence obtained in previous study. A 1 585-bp cDNA fragment was amplified and cloned. The 1 585-bp cDNA contains an open reading frame (ORF) comprising of 1 533 nucleotides (nt) which encodes a 511 residue polypepetides, including 67 amino acids chloroplast transit peptide and 444 amino acids EPSP synthase mature peptide. A comparison between the EPSP synthase of different sources indicates that the mature peptide shows more than 51% identity except for the fungi EPSP synthase and the transit peptide shows considerably less sequence conservation. The copy number of rice epsps gene is estimated to be one copy per haploid rice genome using southern blot. RT-PCR indicated that rice epsps gene is expressed in rice leaves, endosperms and roots and has the highest expression level in leaves.展开更多
[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DN...[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.展开更多
文摘To estimate the impact of copying on the indoor air quality, and to investigate whether ozone emitted during such a process induces pathological oxidative stress and potential oxidative damage in the bodies of operators. Methods 67 copying operators (CO) and 67 healthy volunteers (HV) were enrolled in a random control study, in which levels of lipoperoxide (LPO) in plasma and erythrocytes, and levels of vitamin C (VC), vitamin E (VE) and b-carotene (b-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined by spectrophotometric methods. Results Compared with the HV group, the average values of LPO in plasma and erythrocytes in the CO group were significantly increased (P<0.0001), while those of VC, VE and b-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the CO group were significantly decreased (P<0.0001). Pearson product-moment correlation analysis showed that with increase of ozone level in copying sites and duration of exposure to ozone, the values of LPO in plasma and erythrocytes in the bodies of operators were gradually increased,while those of VC, VE, b-CAR, SOD, CAT and GPX were decreased in the same manner. Odds ratio (OR) of risk of biochemical parameters reflecting potential oxidative damage of the copying operators ranged from 4.440 to 13.516, and 95 % CI of OR was from 2.113 to 34.061. Reliability coefficient () of the biochemical parameters used to reflect the potential oxidative damage of the operators was 0.8156, standardized item =0.9929, P<0.0001. Conclusion Findings in the present study suggest that there exist a series of free radical chain reactions and pathological oxidative stress induced by high dose ozone in the operators, thereby causing potential oxidative and lipoperoxidative damages in their bodies.
文摘Mate-choice copying is a fascinating and widespread mate-choice strategy. Individuals gather public information about potential mates by observing others during sexual interactions and choose or reject the same individual as a mate as the observed individual did before. The influence of copying behavior on an individual＇s mate choice can be so strong that socially acquired information can override genetically based preferences for certain phenotypes. Thus, mate-choice copying enforces dy- namic processes in sexual selection. Here, we review the current state of research on mate-choice copying and focus on sex-specific aspects. We present evidence that mate-choice copying can support the evolution of novel sexual ornaments, and we discuss potential costs of mate-choice copying when public information is not reliable. Moreover, we discuss the conflict faced by males that copy since mate-choice copying increases sperm competition. In conclusion we suggest interesting topics for future research in mate-choice copying .
文摘A criterion is proposed to the feasibility on radial copying grinding, i.e.the pressure angleapplying to a point on the workpiece contour to be profiled should be smaller than its angle limit.Therefore, the expressions of the angle applying to copying grinding and the angle limit to copyingmechanism are derived, with the measures taken for the quality improvement of copying movementin contour grinding discussed.
基金R.T.G.was partially supported by Natural Environment Research Council(grant no.NE/L002469/1).
文摘Mate choice copying-when individuals learn to prefer mates or mate types that have been chosen by others-can influence trait evo-lution and speciation(Varela et al.2018;Dion et al.2019).Most examples of mate choice copying are from fish,birds,and mammals including humans(Varela et al.2018).However,2 invertebrate examples-fruit flies and wolf spiders-have been used to argue that the phenomenon may be phylogenetically widespread,and perhaps the rule rather than the exception in nature(Varela et al.2018).Here,we revisit the evidence for mate choice copying in wolf spiders(Fowler-Fimn et al.2015)in light of new data(Gilman et al.2018).Then,we discuss why mate choice copying is a phenomenon that is likely to occur in wolf spiders,and why this deserves attention.
基金The project supported in part by the National Fundamental Research Program, National Natural Science Foundation of China, EYTFof the Educational Department of China, and Hunan Province STF
文摘We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubitsystem. There may exist inter-three-qubit entanglement and inter-two-qubit entanglement for the three-qubit system.We show that both local and non-local copying processes degrade quantum entanglement in the three-particle systemdue to a residual correlation between the copied output and the copying machine. We also show that the inter-two-qubitentanglement is preserved better than the inter-three-qubit entanglement in the local cloning process. We find thatnon-local cloning is much more efficient than the local copying for broadcasting entanglement, and output state vianon-local cloning exhibits the fidelity better than local cloning.
基金the National Fundamental Research Program，国家自然科学基金，教育部EYTF项目，湖南省科研项目
文摘We study the degree to which quantum entanglement survives when a three-qubit entangled state iscopied by using local and non-local processes, respectively, and investigate iterating quantum copyingfor the three-qubitsystem. There may exist inter-three-qubit entanglement and inter-two-qubit entanglement for the three-qubit system.We show that both local and non-local copying processes degrade quantum entanglement in the three-particle systemdue to a residual correlation between the copied output and the copying machine. We also show that the inter-two-qubitentanglement is preserved better than the inter-three-qubit entanglement in the local cloning process. We find thatnon-local cloning is much more efficient than the local copying for broadcasting entanglement, and output state vianon-local cloning exhibits the fidelity better than local cloning.
文摘Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of upper and lower motor neurons in the brainstem and spinal cord,leading to muscle weakness,paralysis,and respiratory failure (Morgan and Orrell,2016).
文摘In order to celebrate its second anniversary,a shopping center in Guiyang,capital of southwest China’s Guizhou Province,organized a tomato fight on July 20.The idea was based on the famed tomato festivalin Spain.
基金supported in part by the National Science Foundation of China,under grant numbers T2122001 and 12203049in part by the Key Research Program of Frontier Sciences,CAS,under grant number ZDBS-LY-JSC044in part by the Youth Innovation Promotion Association of the Chinese Academy of Sciences under Grant number 2023230.
文摘Implementation of robot-based motion control in optical machining demonstrably enhances the machining quality.The introduction of motion-copying method enables learning and replicating manipulation from experienced technicians.Nevertheless,the location uncertainties of objects and frequent switching of manipulated spaces in practical applications impose constraints on their further advancement.To address this issue,a motion-copying system with a symbol-sequence-based phase switch control(SSPSC)scheme was developed by transferring the operating skills and intelligence of technicians to mechanisms.The manipulation process is decomposed,symbolised,rearranged,and reproduced according to the manufacturing characteristics regardless of the change in object location.A force-sensorless adaptive sliding-mode-assisted reaction force observer(ASMARFOB),wherein a novel dual-layer adaptive law was designed for high-performance fine force sensing,was established.The uniformly ultimate boundedness(UUB)of the ASMARFOB is guaranteed based on the Lyapunov stability theory,and the switching stability of the SSPSC was examined.Validation simulations and experiments demonstrated that the proposed method enables better motion reproduction with high consistency and adaptability.The findings of this study can provide effective theoretical and practical guidance for high-precision intelligent optical manufacturing.
基金funded by the National Natural Science Foundation of China(grant no.32270238 and 31870311).
文摘Subtropical evergreen broad-leaved trees are usually vulnerable to freezing stress,while hexaploid wild Camellia oleifera shows strong freezing tolerance.As a valuable genetic resource of woody oil crop C.oleifera,wild C.oleifera can serve as a case for studying the molecular bases of adaptive evolution to freezing stress.Here,47 wild C.oleifera from 11 natural distribution sites in China and 4 relative species of C.oleifera were selected for genome sequencing.“Min Temperature of Coldest Month”(BIO6)had the highest comprehensive contribution to wild C.oleifera distribution.The population genetic structure of wild C.oleifera could be divided into two groups:in cold winter(BIO6≤0℃)and warm winter(BIO6>0℃)areas.Wild C.oleifera in cold winter areas might have experienced stronger selection pressures and population bottlenecks with lower N_(e) than those in warm winter areas.155 singlenucleotide polymorphisms(SNPs)were significantly correlated with the key bioclimatic variables(106 SNPs significantly correlated with BIO6).Twenty key SNPs and 15 key copy number variation regions(CNVRs)were found with genotype differentiation>50%between the two groups of wild C.oleifera.Key SNPs in cis-regulatory elements might affect the expression of key genes associated with freezing tolerance,and they were also found within a CNVR suggesting interactions between them.Some key CNVRs in the exon regions were closely related to the differentially expressed genes under freezing stress.The findings suggest that rich SNPs and CNVRs in polyploid trees may contribute to the adaptive evolution to freezing stress.
文摘Hua Shou(1304-1386)styled himself Ying Ning Sheng.He was also known as Hua Boren.His ancestral home was in Xiang Town,Xu Prefecture(present Xuchang,Henan province).His birth place was Yizhen,Jiangsu province.Later he moved to Yuyao,Zhejiang province.He was one of the distinguished practitioners of the late Yuan and early Ming dynasties.His writings include Elaboration of the Fourteen Meridians(Shi Si Jing Fa Hui),Notes on Handwritten Copy of Basic Questions(Du Su Wen Chao),The Essentials for Physicians(Zhen Jia Shu Yao).
基金supported by the French National Research Agency(Agence Nationale de la Recherche,ANR,Grant No.ANR-23-CE37-0014)for the Effort_(LESS)Project.
文摘Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise from the brain processing of an efference copy of the motor command in sensory areas.However,recent research suggests that feedback from muscle spindles plays a significant role in this perception.In this study,tendon vibration protocols were employed to attenuate sensory feedback during subsequent cycling exercises.The aim was to assess whether vibration would increase cycling power output,muscle activation,and heart rate at fixed perceived effort intensities.Methods:Fifteen healthy young participants completed 2 experimental visits(vibration and sham).In each visit,participants performed two 3-min cycling bouts,1 at a moderate perceived effort intensity and 1 at a strong perceived effort intensity,before(pre)and after(post)an actual or a sham vibration protocol.Vibration was applied bilaterally on the patellar and Achilles tendons for 10 min.Power output,heart rate,and vastus lateralis electromyography(VL EMG)were recorded and averaged for each bout.Absolute values as well as relative change(%)between pre and post conditions were compared across sham and vibration conditions.Results:At moderate perceived effort,power output,heart rate,and VL EMG increased post-vibration compared to pre-vibration(p<0.05),while no difference was observed in the sham condition.At strong perceived effort,power output and VL EMG decreased post-sham(p<0.05)but remained unchanged post-vibration.Moreover,the relative change between pre and post conditions was significantly higher in the vibration conditions compared to the sham condition for all variables.Conclusion:This study shows that tendon vibration reduces effort perception during subsequent cycling bouts.This effect is likely a consequence of vibration-induced reduction in muscle spindle reafferent signaling to the brain,but this mechanism remains to be further elucidated.From an applied perspective,these findings highlight tendon vibration as a promising tool for enhancing physical activity engagement.
文摘The mitochondrial DNA copy number(mtDNAcn)plays a vital role in cellular energy metabolism and mitochondrial health.As mitochondria are responsible for adenosine triphosphate production through oxidative phosphorylation,maintaining an appropriate mtDNAcn level is vital for the overall cellular function.Alterations in mtDNAcn have been linked to various diseases,including neurodegenerative disorders,metabolic conditions,and cancers,making it an important biomarker for understanding the disease pathogenesis.The accurate estimation of mtDNAcn is essential for clinical applications.Quantitative polymerase chain reaction and next-generation sequencing are commonly employed techniques with distinct advantages and limitations.Clinically,mtDNAcn serves as a valuable indicator for early diagnosis,disease progression,and treatment response.For instance,in oncology,elevated mtDNAcn levels in blood samples are associated with tumor aggressiveness and can aid in monitoring treatment efficacy.In neurodegenerative diseases such as Alzheimer’s and Parkinson’s,altered mtDNAcn patterns provide insights into disease mechanisms and progression.Understanding and estimating mtDNAcn are critical for advancing diagnostic and therapeutic strategies in various medical fields.As research continues to uncover the implications of mtDNAcn alterations,its potential as a clinical biomarker is likely to expand,thereby enhancing our ability to diagnose and manage complex diseases.
基金supported by the National Natural Science Foundation of China(Nos.U200420282273603 and 82003427).
文摘Toxic effects in terms of mitochondria and hereditary substances have been characterized in vitro for individual rare earth elements,while,the joint effects of mixed elements exposure in the population remain ambiguous.Based on the Occupational Chromate Exposure Dynamic Cohort of China,this study investigated the relationship between 15 blood rare earth elements(cerium,dysprosium,erbium,europium,gadolinium,holmium,lanthanum,lutetium,neodymium,praseodymium,samarium,terbium,thulium,yttrium,and ytterbium)and mitochondrial DNA copy number(MtDNACN)as well as peripheral blood lymphocyte micronucleus frequency(MNF).The elastic net was used to select elements highly correlated with effect indicators,whose dose-response relationships were further illustrated by restricted cubic splines.Bayesian kernel regressionwas employed to explore the combined effects of elements and the contributions of single element.The results showed that most rare earth elementswere positively correlatedwith effect indicators,with yttrium showing the strongest association(β(95%CI):0.139(0.1089–0.189)for MtDNACN,0.937(0.345–1.684)for MNF).In the mixed exposure model,with the exposure level fixed at the 50th percentile as the reference,the effect estimates on MtDNACN and MNF increased by 0.228 and 0.598 units,respectively,at the 75th percentile.The single effect analysis implied that yttrium,lanthanum and terbium contributed the most to the elevation of MtDNACN,while yttrium posed the highest risk for genetic damage,accordingly,we provided recommendations to prioritize these elements of concern.In addition,we observed a chief mediating effect of MtDNACN on the elevation of MNF caused by lanthanum,whereas further mechanistic exploration is required to confirm this finding.
基金supported by the National Natural Science Foundation of China(21173039)Pujiang Foundation of Shanghai,China(08PJ14096)+3 种基金Natural Science Foundation of Shanghai Science and Technology Committee,China(09ZR1433300)Opening Foundation of Zhejing Provincial Top Key Discipline,China(20110927)Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China(2009(1001))Shanghai Leading Academic Discipline Project Fund,China(B604)~~
文摘In order to isolate the total cDNA of rice (Oryza sativa L.) epsps gene, RT-PCR was carried out with template of rice first-strand cDNA and primers designed according to rice EPSP synthase genomic sequence obtained in previous study. A 1 585-bp cDNA fragment was amplified and cloned. The 1 585-bp cDNA contains an open reading frame (ORF) comprising of 1 533 nucleotides (nt) which encodes a 511 residue polypepetides, including 67 amino acids chloroplast transit peptide and 444 amino acids EPSP synthase mature peptide. A comparison between the EPSP synthase of different sources indicates that the mature peptide shows more than 51% identity except for the fungi EPSP synthase and the transit peptide shows considerably less sequence conservation. The copy number of rice epsps gene is estimated to be one copy per haploid rice genome using southern blot. RT-PCR indicated that rice epsps gene is expressed in rice leaves, endosperms and roots and has the highest expression level in leaves.
基金Funded by Program of Technology Bureau of Harbin(2010RFQXN101)Sub-project of Transgenic Significant Specific Project(2008ZX08012-001)~~
文摘[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.
