Magnesium-doped calcium silicate(CS)bioceramic scaffolds have unique advantages in mandibular defect repair;however,they lack antibacterial properties to cope with the complex oral microbiome.Herein,for the first time...Magnesium-doped calcium silicate(CS)bioceramic scaffolds have unique advantages in mandibular defect repair;however,they lack antibacterial properties to cope with the complex oral microbiome.Herein,for the first time,the CS scaffold was functionally modified with a novel copper-containing polydopamine(PDA(Cu^(2+)))rapid deposition method,to construct internally modified(*P),externally modified(@PDA),and dually modified(*P@PDA)scaffolds.The morphology,degradation behavior,and mechanical properties of the obtained scaffolds were evaluated in vitro.The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance.During the prolonged immersion stage,the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release.The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA,while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects.Finally,the PDA(Cu^(2+))-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model.Overall,it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu^(2+))modification,and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.展开更多
Infrared spectral studies of copper-containing film from steel sample (worn in pair with brass in glycerol) friction zone are performed in the study. The protective film formed by the interaction of the friction triad...Infrared spectral studies of copper-containing film from steel sample (worn in pair with brass in glycerol) friction zone are performed in the study. The protective film formed by the interaction of the friction triad having functional groups typical for macromolecular compounds is shown.展开更多
Bone-related diseases pose a major challenge in contemporary society,with significant implications for both health and economy.Copper,a vital trace metal in the human body,facilitates a wide range of physiological pro...Bone-related diseases pose a major challenge in contemporary society,with significant implications for both health and economy.Copper,a vital trace metal in the human body,facilitates a wide range of physiological processes by being crucial for the function of proteins and enzymes.Numerous studies have validated copper's role in bone regeneration and protection,particularly in the development and expansion of bone collagen.Owing to copper's numerous biological advantages,an increasing number of scientists are endeavoring to fabricate novel,multifunctional copper-containing biomaterials as an effective treatment strategy for bone disorders.This review integrates the current understanding regarding the biological functions of copper from the molecular and cellular levels,highlighting its potential for bone regeneration and protection.It also reviews the novel fabrication techniques for developing copper-containing biomaterials,including copper-modified metals,calcium phosphate bioceramics,bioactive glasses,bone cements,hydrogels and biocomposites.The fabrication strategies and various applications of these biomaterials in addressing conditions such as fractures,bone tumors,osteomyelitis,osteoporosis,osteoarthritis and osteonecrosis are carefully elaborated.Moreover,the long-term safety and toxicity assessments of these biomaterials are also presented.Finally,the review addresses current challenges and future prospects,in particular the regulatory challenges and safety issues faced in clinical implementation,with the aim of guiding the strategic design of multifunctional copper-based biomaterials to effectively manage bone-related diseases.展开更多
Large-scale growth of copper oxide nanowires was realized on surfaces of various copper-containing substrates, including copper grids, high-purity copper foils, and small copper blocks, by the stress-induced method. A...Large-scale growth of copper oxide nanowires was realized on surfaces of various copper-containing substrates, including copper grids, high-purity copper foils, and small copper blocks, by the stress-induced method. A relatively low heating temperature of 340 ~C was demonstrated to give rise to dense nanowire growth with fine crystal structures and high aspect ratio of approximately 300. Gradual cooling process, which is positive for the growth of nanowires on multi-layer substrates, is shown to have no effect on the nanowire growth on other pure copper substrates. Diameter of as-obtained nanowires is mainly dependent on the heating temperature. Moreover, the nanowires growing on copper grids are much longer than those growing on two other substrates.展开更多
This research was to assess the dietary copper(Cu)requirement of broiler chickens fed a practical cornsoybean meal diet during 22—42 d of age.A total of 288 numbered Arbor Acres male broilers at 22 d of age were rand...This research was to assess the dietary copper(Cu)requirement of broiler chickens fed a practical cornsoybean meal diet during 22—42 d of age.A total of 288 numbered Arbor Acres male broilers at 22 d of age were randomly allotted 6 treatments with 8 replicate cages(6 broilers per cage)per treatment.Broilers were fed a Cu-unsupplemented corn-soybean meal basal diet(control,containing 7.36 mg Cu/kg)or the basal diet added with 3,6,9,12,or 15 mg Cu/kg from CuSO_(4)·5H_(2)O for 21 d.Quadratic,asymptotic and broken-line models were fitted and the best fitted models were selected to determine dietary Cu requirements.The results revealed that the contents of Cu in serum and liver,m RNA expression levels of Cu-and zinc-containing superoxide dismutase(CuZnSOD)in liver and monoamine oxidase b(MAO B)in heart,as well as protein expression level of CuZnSOD in liver were affected(P<0.05)by supplemental Cu levels,and the above indices varied linearly and quadratically(P<0.05)with increasing Cu levels.Dietary Cu requirements assessed according to the best fitted broken-line models(P<0.05)of the above indexes were 10.45—13.81 mg/kg.It was concluded that mRNA expression levels of CuZnSOD in liver and MAO B in heart,as well as liver CuZnSOD protein expression level were new specific sensitive biomarkers for estimating dietary Cu requirements,and the dietary Cu requirement was recommended to be 14 mg/kg to support Cu metabolic needs related to key Cucontaining enzymes in broilers fed the corn-soybean meal diet during 22—42 d of age,which was higher than the dietary Cu requirement(8 mg/kg)for broilers at the corresponding stage suggested by the Chinese Feeding Standard of Chicken.展开更多
基金supported by the Key Research and Development Program of Zhejiang Province Foundation(No.2019C03027)the Zhejiang Provincial Natural Science Foundation of China(No.LZ22E020002)+1 种基金the Scientific Research Fund of Zhejiang Provincial Education Department(No.Y202148333)the Zhejiang Provincial Basic Research for Public Welfare Funds(Nos.LGF22E030002,LGF21H140001,and LTGY23H140005),China.
文摘Magnesium-doped calcium silicate(CS)bioceramic scaffolds have unique advantages in mandibular defect repair;however,they lack antibacterial properties to cope with the complex oral microbiome.Herein,for the first time,the CS scaffold was functionally modified with a novel copper-containing polydopamine(PDA(Cu^(2+)))rapid deposition method,to construct internally modified(*P),externally modified(@PDA),and dually modified(*P@PDA)scaffolds.The morphology,degradation behavior,and mechanical properties of the obtained scaffolds were evaluated in vitro.The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance.During the prolonged immersion stage,the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release.The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA,while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects.Finally,the PDA(Cu^(2+))-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model.Overall,it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu^(2+))modification,and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.
文摘Infrared spectral studies of copper-containing film from steel sample (worn in pair with brass in glycerol) friction zone are performed in the study. The protective film formed by the interaction of the friction triad having functional groups typical for macromolecular compounds is shown.
基金financed by the Third People's Hospital Independent Research Project[No.CSY-YN-01-2023-017]Chengdu Medical Research Project[No.2023021]+2 种基金Southwest Jiaotong University Medical and Industrial Training Project[No.2682024ZTPY001]National Natural Science Foundation of China[32471402]National Natural Science Foundation of China[52071277].
文摘Bone-related diseases pose a major challenge in contemporary society,with significant implications for both health and economy.Copper,a vital trace metal in the human body,facilitates a wide range of physiological processes by being crucial for the function of proteins and enzymes.Numerous studies have validated copper's role in bone regeneration and protection,particularly in the development and expansion of bone collagen.Owing to copper's numerous biological advantages,an increasing number of scientists are endeavoring to fabricate novel,multifunctional copper-containing biomaterials as an effective treatment strategy for bone disorders.This review integrates the current understanding regarding the biological functions of copper from the molecular and cellular levels,highlighting its potential for bone regeneration and protection.It also reviews the novel fabrication techniques for developing copper-containing biomaterials,including copper-modified metals,calcium phosphate bioceramics,bioactive glasses,bone cements,hydrogels and biocomposites.The fabrication strategies and various applications of these biomaterials in addressing conditions such as fractures,bone tumors,osteomyelitis,osteoporosis,osteoarthritis and osteonecrosis are carefully elaborated.Moreover,the long-term safety and toxicity assessments of these biomaterials are also presented.Finally,the review addresses current challenges and future prospects,in particular the regulatory challenges and safety issues faced in clinical implementation,with the aim of guiding the strategic design of multifunctional copper-based biomaterials to effectively manage bone-related diseases.
文摘Large-scale growth of copper oxide nanowires was realized on surfaces of various copper-containing substrates, including copper grids, high-purity copper foils, and small copper blocks, by the stress-induced method. A relatively low heating temperature of 340 ~C was demonstrated to give rise to dense nanowire growth with fine crystal structures and high aspect ratio of approximately 300. Gradual cooling process, which is positive for the growth of nanowires on multi-layer substrates, is shown to have no effect on the nanowire growth on other pure copper substrates. Diameter of as-obtained nanowires is mainly dependent on the heating temperature. Moreover, the nanowires growing on copper grids are much longer than those growing on two other substrates.
基金supported by Jiangsu Shuang Chuang Tuan Dui program(JSSCTD202147)Jiangsu Shuang Chuang Ren Cai program(JSSCRC2021541)+1 种基金Young Elite Scientists Sponsorship Program by CAST(2022QNRC001)the Initiation Funds of Yangzhou University for Distinguished Scientists
文摘This research was to assess the dietary copper(Cu)requirement of broiler chickens fed a practical cornsoybean meal diet during 22—42 d of age.A total of 288 numbered Arbor Acres male broilers at 22 d of age were randomly allotted 6 treatments with 8 replicate cages(6 broilers per cage)per treatment.Broilers were fed a Cu-unsupplemented corn-soybean meal basal diet(control,containing 7.36 mg Cu/kg)or the basal diet added with 3,6,9,12,or 15 mg Cu/kg from CuSO_(4)·5H_(2)O for 21 d.Quadratic,asymptotic and broken-line models were fitted and the best fitted models were selected to determine dietary Cu requirements.The results revealed that the contents of Cu in serum and liver,m RNA expression levels of Cu-and zinc-containing superoxide dismutase(CuZnSOD)in liver and monoamine oxidase b(MAO B)in heart,as well as protein expression level of CuZnSOD in liver were affected(P<0.05)by supplemental Cu levels,and the above indices varied linearly and quadratically(P<0.05)with increasing Cu levels.Dietary Cu requirements assessed according to the best fitted broken-line models(P<0.05)of the above indexes were 10.45—13.81 mg/kg.It was concluded that mRNA expression levels of CuZnSOD in liver and MAO B in heart,as well as liver CuZnSOD protein expression level were new specific sensitive biomarkers for estimating dietary Cu requirements,and the dietary Cu requirement was recommended to be 14 mg/kg to support Cu metabolic needs related to key Cucontaining enzymes in broilers fed the corn-soybean meal diet during 22—42 d of age,which was higher than the dietary Cu requirement(8 mg/kg)for broilers at the corresponding stage suggested by the Chinese Feeding Standard of Chicken.
