Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract...Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.展开更多
Accumulating evidence has demonstrated that endophytic fungi metabolites possess important biological activities.Ageratum conyzoides L.(Asteraceae)is an invasive aromatic herb with immense therapeutic importance.In th...Accumulating evidence has demonstrated that endophytic fungi metabolites possess important biological activities.Ageratum conyzoides L.(Asteraceae)is an invasive aromatic herb with immense therapeutic importance.In the present study,we aimed to isolate and identify endophytic fungi in the roots,stems,leaves and flowers of Ageratum conyzoides L.and evaluate its antioxidant activity(DPPH,ABTS^(+) and FRAP assays),the contents of flavonoids and phenolic and antibacterial activity of their ethyl acetate extracts.Accordingly,a total of 25 endophytic fungi were isolated from Ageratum conyzoides L.and classified into 14 genera based on the Internal transcribed spacer sequence,among which Fusarium sp.(20%)were found to be the dominant groups.All fungal extracts exhibited antioxidant activity,and the extracts of fungi WZ07 exhibited antioxidant activities as well as higher flavonoids and phenolic content in all tests.Moreover,the extracts of fungi WL01 had good antibacterial activity against both Gram-positive and Gram-negative bacteria.We,for the first time,reported the antioxidant and antibacterial activity of endophytic fungi from Ageratum conyzoides L.,which may help lay the foundation for developing other medicinal components.展开更多
Sustainable weed management strategies are essential to reduce chemical and labor inputs. This study aimed to evaluate the effect of water extracts from sweet potato [Ipomoea batatas (L.) Lamarck] on seed germination ...Sustainable weed management strategies are essential to reduce chemical and labor inputs. This study aimed to evaluate the effect of water extracts from sweet potato [Ipomoea batatas (L.) Lamarck] on seed germination of Ageratum conyzoides L. under controlled conditions. The aqueous was produced from plant parts i.e., roots, stems, and leaves of sweet potato at concentrations of 0.025, 0.050, 0.075, and 0.100 g·mL<sup>-1</sup>. The results showed that the plant parts of sweet potato all contained allelopathic substances, which showed high-concentration inhibition and low-concentration promotion of seed germination of A. conyzoides. When the aqueous extract concentrations were 0.050, 0.075, and 0.100 g·mL<sup>-1</sup>, the germination of A. conyzoides seeds was inhibited, while the germination was promoted at a concentration of 0.025 g·mL<sup>-1</sup>. This shows that when the planting density of sweet potato is large, it can form an obvious prevention and control effect on A. conyzoides, and thus improve herbicide resistance management.展开更多
The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suav...The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’ oral bacteria. Healthy green leaves of the plants were collected in Ishaka Uganda, processed and portions separately extracted with hot distilled water and cold ethanol. The susceptibility, MIC and MBC of each extract were determined using standard protocols. The bacteria had significant (p Bidens pilosa, Ageratum conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by traditional healers and local people in South-Western Uganda. We recommend a detailed study of structural identities and activities of the active antibacterial principle(s) in these plants for possible new drug entities and verification of the interactive effects of the principle(s) with ARVs and cotrimoxazole used daily by HIV/AIDS patients.展开更多
Cowpea is a vegetable that contributes to food security in Guinea. Despite its importance, more than 30% of its production is lost between harvesting, storage and consumption. The main cowpea pest is Callosobruchus ma...Cowpea is a vegetable that contributes to food security in Guinea. Despite its importance, more than 30% of its production is lost between harvesting, storage and consumption. The main cowpea pest is Callosobruchus maculatus. The commonly used control strategy is essentially based on chemicals whose use is toxic, expensive and restrictive. In the search for alternatives to chemical control, this work was undertaken to evaluate the insecticidal potential of Ageratum conyzoid leaf powders and Securidaca longepedunculata roots. Powder toxicity and insecticidal efficacy tests were performed separately on groups of 20 Callosobruchus maculatus using 3 doses of Ageratum conyzoid leaf powders and Securidaca longepedunculata roots in jars each containing 100 g cowpea. The mortality of Callosobruchus maculatus and the impact of powders were noted respectively for 96 h and 4 months after infestation. The germination capacity of the treated seeds was assessed at the start of the study. Doses of 1 and 8 g of Ageratum conyzoides leaf powders and Securidaca longepedunculata roots were applied respectively to every 100 g of cowpea and led to 100% mortality of Callosobruchus maculatus. At these same doses, more than half of the treated seeds were attacked during the 4 months of storage. Germination tests have shown that powders of Ageratum conyzoides leaves and roots of Securidaca longepedunculata have no negative effect on the germination power of cowpea seeds. Therefore, they could be considered as excellent bio-insecticides that socio-professional strata (farmers and warehouse workers) can use in the fight against cowpea insects intended for storage.展开更多
[Objcetives]To study on the original plant,medicinal materials and microscopic identification of Yi Nationality crude herb Te-zhipa of Ageratum conyzoides L.,and provide experimental data for identification and applic...[Objcetives]To study on the original plant,medicinal materials and microscopic identification of Yi Nationality crude herb Te-zhipa of Ageratum conyzoides L.,and provide experimental data for identification and application. [Methods] The original plant identification,character identification,microscopic structure identification,powder identification were adopted in theresearch.[Results] The characteristics of Yi Nationality crude herb Te-zhi-pa were obvious in the original plant,crude herband microscopic characteristics. [Conclusions]The result was provided some reference for the formulation of quality standards and original plant identification for Yi Nationality crude herb " Te-zhi-pa",and provided the basis for the further research.展开更多
Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and JEE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the acti...Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and JEE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver enzymes.Methods:Acute toxicity of JME and JEE was determined using Lorke’s method.In vitro and in vivo opening of the mitochondrial membrane permeability transition pore(MMPT pore)was spectrophotometrically assayed.Production of malondialdehyde(MDA)as an index of lipid peroxidation and the activity of mitochondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase(ALP),aspartate and alanine aminotransferase(AST and ALT)and gamma-glutamyl transferase(GGT)was also investigated.Results:JME had an LD_(50) of 3808 mg/kg b.w whereas JEE had an LD_(50) greater than 5000 mg/kg b.w.of rats.After the rats have been fed with both extracts,a photomicrograph of a piece of liver tissue showed no apparent symptoms of toxicity.From the in vitro and in vivo studies,both extracts prompted intact mitochondria to open their MMPT pores.When compared to the control,lipid peroxide product release and ATPase activity were significantly increased(P<0.05)in vitro and in vivo.The activities of AST,ALT,and GGT were all reduced at 50 mg/kg when treated with JME,but the activity of AST was considerably enhanced when treated with JEE(P<0.05).The results revealed that both JME and JEE of the Jatonik polyherbal mixture had low toxicity,profound MMPTpore induction,and enhanced ATPase activity,but an increased MDA production.Conclusion:Jatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis,however,further studies are needed to better clarify the molecular mechanism involved in these phenomena.展开更多
基金supported by the Ministry of Research Technology and Higher Education of Indonesia 2016
文摘Objective:To analyze the effects of Ageratum conyzoides L.on the monosodium iodoacetate induced osteoarthritis rats.Methods:Thin layer chromatography was performed to analyze the constituents of the babandotan extract leaves.White male Sprague-Dawley rats used in this study were divided into 6 groups:normal control and negative control groups,both given0.5%carboxymethyl cellulose;the positive control group that was given glucosamine and chondroitin suspension(486 mg/200 g B.W.);the 3 dose variation extract groups including dose 1,2,and 3 that were given 40,80,and 160 mg/200 g B.W.respectively on day 29 until50.All the groups were induced with 0.05 mL monosodium iodoacetate(20 mg/mL)on day1,except normal control induced by saline.Measurement of edema volume of rat knees was performed on day 0,8,15,22,29,43,and 50.Hematology data was measured at day 1,29and 50.Serum was collected at day 50 to evaluate TNF-α and MMP-9 by ELISA.Cartilage histopathology was evaluated by staining with H&E and Safranin-o-fast green staining on day 50.Results:The babandotan leaves extract dose 2(80 mg/200 g B.W.)and dose 3(160mg/200 g B.W.)could decrease the edema volume,increase the area and thickness of articular cartilage,and increase proteoglycan level.Particularly,dose 3(160 mg/200 g B.W.)of extract babandotan leaves were able to significantly decrease the number of leukocytes,lymphocytes and udem volume,and decrease TNF alpha and MMP-9 levels.Conclusions:Babandotan leaves extract can recover inflammation and cartilages degradation by inhibiting TNF-αin inflammation processes and MMP-9 in the collagenase reaction in the cartilages.
基金National Natural Science Foundation of China(Grant No.31500162)the National Traditional Chinese Medicine Resources Survey Project for Public Health Service Subsidy for Traditional Chinese Medicine in 2018(Grant No.2018-43)+1 种基金the Natural Science Foundation of Fujian Province(Grant No.2020J01078)Project for Postgraduates’Innovative Fund in Scientific Research of Huaqiao University(Grant No.18014087015)。
文摘Accumulating evidence has demonstrated that endophytic fungi metabolites possess important biological activities.Ageratum conyzoides L.(Asteraceae)is an invasive aromatic herb with immense therapeutic importance.In the present study,we aimed to isolate and identify endophytic fungi in the roots,stems,leaves and flowers of Ageratum conyzoides L.and evaluate its antioxidant activity(DPPH,ABTS^(+) and FRAP assays),the contents of flavonoids and phenolic and antibacterial activity of their ethyl acetate extracts.Accordingly,a total of 25 endophytic fungi were isolated from Ageratum conyzoides L.and classified into 14 genera based on the Internal transcribed spacer sequence,among which Fusarium sp.(20%)were found to be the dominant groups.All fungal extracts exhibited antioxidant activity,and the extracts of fungi WZ07 exhibited antioxidant activities as well as higher flavonoids and phenolic content in all tests.Moreover,the extracts of fungi WL01 had good antibacterial activity against both Gram-positive and Gram-negative bacteria.We,for the first time,reported the antioxidant and antibacterial activity of endophytic fungi from Ageratum conyzoides L.,which may help lay the foundation for developing other medicinal components.
文摘Sustainable weed management strategies are essential to reduce chemical and labor inputs. This study aimed to evaluate the effect of water extracts from sweet potato [Ipomoea batatas (L.) Lamarck] on seed germination of Ageratum conyzoides L. under controlled conditions. The aqueous was produced from plant parts i.e., roots, stems, and leaves of sweet potato at concentrations of 0.025, 0.050, 0.075, and 0.100 g·mL<sup>-1</sup>. The results showed that the plant parts of sweet potato all contained allelopathic substances, which showed high-concentration inhibition and low-concentration promotion of seed germination of A. conyzoides. When the aqueous extract concentrations were 0.050, 0.075, and 0.100 g·mL<sup>-1</sup>, the germination of A. conyzoides seeds was inhibited, while the germination was promoted at a concentration of 0.025 g·mL<sup>-1</sup>. This shows that when the planting density of sweet potato is large, it can form an obvious prevention and control effect on A. conyzoides, and thus improve herbicide resistance management.
文摘The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’ oral bacteria. Healthy green leaves of the plants were collected in Ishaka Uganda, processed and portions separately extracted with hot distilled water and cold ethanol. The susceptibility, MIC and MBC of each extract were determined using standard protocols. The bacteria had significant (p Bidens pilosa, Ageratum conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by traditional healers and local people in South-Western Uganda. We recommend a detailed study of structural identities and activities of the active antibacterial principle(s) in these plants for possible new drug entities and verification of the interactive effects of the principle(s) with ARVs and cotrimoxazole used daily by HIV/AIDS patients.
文摘Cowpea is a vegetable that contributes to food security in Guinea. Despite its importance, more than 30% of its production is lost between harvesting, storage and consumption. The main cowpea pest is Callosobruchus maculatus. The commonly used control strategy is essentially based on chemicals whose use is toxic, expensive and restrictive. In the search for alternatives to chemical control, this work was undertaken to evaluate the insecticidal potential of Ageratum conyzoid leaf powders and Securidaca longepedunculata roots. Powder toxicity and insecticidal efficacy tests were performed separately on groups of 20 Callosobruchus maculatus using 3 doses of Ageratum conyzoid leaf powders and Securidaca longepedunculata roots in jars each containing 100 g cowpea. The mortality of Callosobruchus maculatus and the impact of powders were noted respectively for 96 h and 4 months after infestation. The germination capacity of the treated seeds was assessed at the start of the study. Doses of 1 and 8 g of Ageratum conyzoides leaf powders and Securidaca longepedunculata roots were applied respectively to every 100 g of cowpea and led to 100% mortality of Callosobruchus maculatus. At these same doses, more than half of the treated seeds were attacked during the 4 months of storage. Germination tests have shown that powders of Ageratum conyzoides leaves and roots of Securidaca longepedunculata have no negative effect on the germination power of cowpea seeds. Therefore, they could be considered as excellent bio-insecticides that socio-professional strata (farmers and warehouse workers) can use in the fight against cowpea insects intended for storage.
基金Supported by National Science and Technology Support Program(2015BAC05B02)Sichuan Science and Technology Support Program(2015SZ0034)Education and Teaching Research and Refrom Program of Southwest Minzu University(2015)
文摘[Objcetives]To study on the original plant,medicinal materials and microscopic identification of Yi Nationality crude herb Te-zhipa of Ageratum conyzoides L.,and provide experimental data for identification and application. [Methods] The original plant identification,character identification,microscopic structure identification,powder identification were adopted in theresearch.[Results] The characteristics of Yi Nationality crude herb Te-zhi-pa were obvious in the original plant,crude herband microscopic characteristics. [Conclusions]The result was provided some reference for the formulation of quality standards and original plant identification for Yi Nationality crude herb " Te-zhi-pa",and provided the basis for the further research.
文摘Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and JEE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver enzymes.Methods:Acute toxicity of JME and JEE was determined using Lorke’s method.In vitro and in vivo opening of the mitochondrial membrane permeability transition pore(MMPT pore)was spectrophotometrically assayed.Production of malondialdehyde(MDA)as an index of lipid peroxidation and the activity of mitochondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase(ALP),aspartate and alanine aminotransferase(AST and ALT)and gamma-glutamyl transferase(GGT)was also investigated.Results:JME had an LD_(50) of 3808 mg/kg b.w whereas JEE had an LD_(50) greater than 5000 mg/kg b.w.of rats.After the rats have been fed with both extracts,a photomicrograph of a piece of liver tissue showed no apparent symptoms of toxicity.From the in vitro and in vivo studies,both extracts prompted intact mitochondria to open their MMPT pores.When compared to the control,lipid peroxide product release and ATPase activity were significantly increased(P<0.05)in vitro and in vivo.The activities of AST,ALT,and GGT were all reduced at 50 mg/kg when treated with JME,but the activity of AST was considerably enhanced when treated with JEE(P<0.05).The results revealed that both JME and JEE of the Jatonik polyherbal mixture had low toxicity,profound MMPTpore induction,and enhanced ATPase activity,but an increased MDA production.Conclusion:Jatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis,however,further studies are needed to better clarify the molecular mechanism involved in these phenomena.
