[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta ...[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.展开更多
According to the literature retrieval,the composition,detection method,preparation method and product development of total flavones in Marchantia convoluta were reviewed and prospected. This paper will provide a theor...According to the literature retrieval,the composition,detection method,preparation method and product development of total flavones in Marchantia convoluta were reviewed and prospected. This paper will provide a theoretical basis for further study and product development of total flavones in M. convoluta.展开更多
基金Supported by Natural Science Foundation of China(No.81260620)Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars
文摘[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.
基金Supported by National Natural Science Foundation of China(81260620)Guangxi Province-Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars(J13162)
文摘According to the literature retrieval,the composition,detection method,preparation method and product development of total flavones in Marchantia convoluta were reviewed and prospected. This paper will provide a theoretical basis for further study and product development of total flavones in M. convoluta.
