Strain OH11, a Gram-negative, nonspore forming, rod-shaped bacterium with powerful antagonistic activity, was isolated from rhizosphere of green pepper in Jiangsu Academy of Agricultural Sciences of China and characte...Strain OH11, a Gram-negative, nonspore forming, rod-shaped bacterium with powerful antagonistic activity, was isolated from rhizosphere of green pepper in Jiangsu Academy of Agricultural Sciences of China and characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain OH11 belongs to the Gammaproteobacteria and had the highest degree of sequence similarity to Lysobacter enzymogenes strain C3 （AY074793） （99%）, Lysobacter enzymogenes strain N4-7 （U89965） （99%）, Lysobacter antibioticus strain （AB019582） （97%）, and Lysobacter gummosus strain （AB16136） （97%）. Chemotaxonomic data revealed that strain OHI 1 possesses a quinine system with Q-8 as the predominant compound and C15：0 iso, C17：1 iso ω9c as the predominant iso-branched fatty acids, all of which corroborated the assignment of strain OH11 to the genus Lysobacter. Results of DNA-DNA hybridization and physiological and biochemical tests clearly showed that strain OH11 was classified as Lysobacter enzymogenes. Strain OH11 could produce protease, chitinase, and β-1,3-glucanase. It showed strong in vitro antifungal activity against Rhizoctonia solani, Sclerotinia scletotiorum, and several other phytopathogenic fungi. This is the first report of identification and characterization of Lysobacter enzymogenes as a biological control agent of plant diseases in China.展开更多
The Neogene fluvial reservoir in the Bohai oilfield is one of the leading development horizons for increasing reserves and production in the Bohai oilfield. However, the development of offshore fluvial reservoirs is f...The Neogene fluvial reservoir in the Bohai oilfield is one of the leading development horizons for increasing reserves and production in the Bohai oilfield. However, the development of offshore fluvial reservoirs is faced with the problems of thin reservoir thickness, narrow plane width, rapid lateral change, and thin well pattern. Taking the KLA oilfield as an example, this paper discusses the nuanced characterization and configuration of a single channel controlled by sedimentary facies to guide developing offshore river facies’ narrow channel main control oilfield. Firstly, based on a large number of core data, the acceptable sedimentary facies identification is realized, the sedimentary model of the study area is established, the delicate calibration of logging facies and seismic facies is realized under the constraint of the sedimentary model, and a set of technical methods for nuanced reservoir characterization guided by seismic sedimentology is summarized, to realize the boundary identification of composite channel configuration and further realize the nuanced characterization of the single narrow channel. Based on this set of technology, it guides the smooth implementation of horizontal wells in the oilfield. The drilling encounter rate of the reservoir in the horizontal section of the single well exceeds 90%, ensuring the injection production connectivity and increasing the reserve production rate by more than 10%.展开更多
基金supported by the National High Technology Research and Development Program of China(2006AA10A211)
文摘Strain OH11, a Gram-negative, nonspore forming, rod-shaped bacterium with powerful antagonistic activity, was isolated from rhizosphere of green pepper in Jiangsu Academy of Agricultural Sciences of China and characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain OH11 belongs to the Gammaproteobacteria and had the highest degree of sequence similarity to Lysobacter enzymogenes strain C3 （AY074793） （99%）, Lysobacter enzymogenes strain N4-7 （U89965） （99%）, Lysobacter antibioticus strain （AB019582） （97%）, and Lysobacter gummosus strain （AB16136） （97%）. Chemotaxonomic data revealed that strain OHI 1 possesses a quinine system with Q-8 as the predominant compound and C15：0 iso, C17：1 iso ω9c as the predominant iso-branched fatty acids, all of which corroborated the assignment of strain OH11 to the genus Lysobacter. Results of DNA-DNA hybridization and physiological and biochemical tests clearly showed that strain OH11 was classified as Lysobacter enzymogenes. Strain OH11 could produce protease, chitinase, and β-1,3-glucanase. It showed strong in vitro antifungal activity against Rhizoctonia solani, Sclerotinia scletotiorum, and several other phytopathogenic fungi. This is the first report of identification and characterization of Lysobacter enzymogenes as a biological control agent of plant diseases in China.
文摘The Neogene fluvial reservoir in the Bohai oilfield is one of the leading development horizons for increasing reserves and production in the Bohai oilfield. However, the development of offshore fluvial reservoirs is faced with the problems of thin reservoir thickness, narrow plane width, rapid lateral change, and thin well pattern. Taking the KLA oilfield as an example, this paper discusses the nuanced characterization and configuration of a single channel controlled by sedimentary facies to guide developing offshore river facies’ narrow channel main control oilfield. Firstly, based on a large number of core data, the acceptable sedimentary facies identification is realized, the sedimentary model of the study area is established, the delicate calibration of logging facies and seismic facies is realized under the constraint of the sedimentary model, and a set of technical methods for nuanced reservoir characterization guided by seismic sedimentology is summarized, to realize the boundary identification of composite channel configuration and further realize the nuanced characterization of the single narrow channel. Based on this set of technology, it guides the smooth implementation of horizontal wells in the oilfield. The drilling encounter rate of the reservoir in the horizontal section of the single well exceeds 90%, ensuring the injection production connectivity and increasing the reserve production rate by more than 10%.
