Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combine...Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combined with centrifugal precipitation method was used to complete the inspection of antibiotic ointment,and the final result was analyzed.Results:The application of membrane filtration method combined with centrifugal precipitation method to complete the test of antibiotic ointment has a significant effect.The test solution is milky white.The possibility if it can exert the effect of eliminating antibacterial components,so that Pseudomonas aeruginosa and Staphylococcus caureus can show an affecting state in the positive test tube,and colonies can be found after observation.Conclusion:The application of membrane filtration method combined with centrifugal precipitation method to complete the inspection of antibiotic ointment can achieve satisfactory results.The method is simple to operate and has high feasibility.It is worthy of further promotion and application.展开更多
Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia pl...Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lingam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum. The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control untreated and infested plants.展开更多
Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was...Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was put into a heat treatment machine in pilot plan, and harmless condition in short time was examined. The results indicated, pathogenic indicator bacteria such as coliform bacteria, fecal coliform, Escherichia coli and salmonella were all 106 cfu/g dw at the beginning, died rapidly when cow dung temperature rose to above 50~C, and not detected at 54-68~C for 6-24 h heat treatment. Coliform bacteria and salmonella in heated cow dung were not detected by re-growth culture and enrichment culture examination. Moreover, it was hardly influenced on the fermentation ability of composting microbe, organic decomposition bacteria. During heat treatment, the mesophile decreased rapidly and the thermophile stabilized or increased, and the most of composting microbe were bacillus in cow dung by fluorescence microscope, this indicated that bacillus was dominator and composting microbe in composting process.展开更多
The possibility of employing antagonistic bacteria for the control of tobacco brown spot was studied. Approximately 136 strains of bacteria were isolated from phyllospheres of tobacco and 9 of these possessed high lev...The possibility of employing antagonistic bacteria for the control of tobacco brown spot was studied. Approximately 136 strains of bacteria were isolated from phyllospheres of tobacco and 9 of these possessed high levels of antagonistic properties, They significantly reduced brown spot in detached tobacco leaves when artificially inoculated with Alternaria alternata. Culture filtrate of the most effective bacterial isolate which designated as Tpb88 was shown to be very efficient in inhibiting mycelial growth of A alternata in dual cultures. Culture filtrate of Tpb88 inhibited germination and germ tube elongation of A alternata. The results showed that the culture filtrate directly inhibited spore germination of A alternata, especially during the first hours of the paired cultivation. The rate of antagonistic activity of culture filtrate of Tpb88 depended on its concentration in the mixture. The greatest inhibition of spore germination was ob served at the highest concentration of filtrate （filtrate to fungal spores inocula mixed in proportion 1 ： 50）. These suggest that the hypothetic mechanism of Tpb88 against tobacco brown spot is to produce antagonistic substances.展开更多
Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 202...Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 2022. Methodology: During this eleven-month prospective study, we set up a quality control device (QCD). For microbiological quality control, we performed different cultures of bacteria with different bacteriological and morphological characteristics at T0 (no sterilization) and at T30 (after 30 minutes of sterilization under DISTER-UV). Results: After the realization, the DCQ attached to the DISTER-UV1 allows to display of the UV wavelength present in the light box. This device also displays and alerts when the UV intensity emitted by the lamps is below 250 nm. During microbiological quality control, the cultures carried out at T30 and incubated for 24 hours did not reveal any bacterial colonies. This shows the bactericidal character of DISTER-UV-2. Conclusion: The improvement and the microbiological quality control allowed us to switch from DISTER-UV1 (without sensor) to DISTER-UV-2 (with sensor or DCQ). The biological control allowed us to affirm that the DISTER-UV-2 is bactericidal.展开更多
Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical a...Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses, were conducted to identify the local isolates of Erwinia amylovora. The alternative antagonistic microorganisms which combat bacterium E. amylovora were tested within in vitro and in vivo conditions. The results revealed the ability of Streptomyces antagonistic bacteria to decrease fire blight severity on pear and apple trees during the first stage of the fire blight disease in leaf tissues. Streptomyces strain C1-4 suppressed E. amylovora disease symptoms in the leaf tissues and excised apple and pear shoots. The incidence of fire blight on leaves was reduced by about 70% with two applications of bacterial antagonists. Further studies at different locations in Kyrgyzstan, using large scale application, would allow for stronger recommendations to be made, including studies and recommendations on their ability to prevent disease and to use them as main components in an integrated pest management program.展开更多
Objective:The acceptability of herbal remedies for alleviating discomforts and ill-health has become very popular, on the account of the increasing cost of allopathic medicine for personal health maintenance.The obser...Objective:The acceptability of herbal remedies for alleviating discomforts and ill-health has become very popular, on the account of the increasing cost of allopathic medicine for personal health maintenance.The observable non-adherence of herbalists to the established World Health Organization(WHO) / National Agency for Food and Drug Administration Control(NAFDAC) regulations for the quality control of herbal medicines is an issue for concern.In view of this,34 popular and widely consumed crude herbal remedies in southwestern,Nigeria were screened for compliance with standard limits for bacterial contamination,bacteria flora and their antibiotic susceptibility pattern.Methods:Isolates recovered from samples were identified using the cultural, morphological and biochemical characteristics.They were also tested for drug sensitivity using standard procedures. Results:A heavy bacteria load ranging from 3.00×10~3-9.58×10~5 CFU/ML and 1.20×10~5- 5.41×10~5 CFU/ML was observed for water and spirit extracted preparations respectively.The bacteria flora cum contaminants were:Staphylococcus aureus,Bacillus cereus,Bacillus subtilis,Pseudomonas aeruginosa, Micrococcus luteus,Lactobacillus plantarum,Klebsiella pneumoniae,Escherichia coli,streptococcus,Shigella, Neisseria,Arthrobacter,Kurthia and Clostridium species.All the isolates were multi-drug resistant(MDR) strains.Conclusion:The crude herbal preparations consumed in Nigeria failed to comply with the internationally recognized standards regarding bacteria load and flora.The presence of MDR pathogens is of greatest concern. It poses a great risk to consumers health and could be a source of introducing MDR organisms into the human population.There is the need for the enforcement of established guidelines to ensure the safety of these preparations.展开更多
文摘Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combined with centrifugal precipitation method was used to complete the inspection of antibiotic ointment,and the final result was analyzed.Results:The application of membrane filtration method combined with centrifugal precipitation method to complete the test of antibiotic ointment has a significant effect.The test solution is milky white.The possibility if it can exert the effect of eliminating antibacterial components,so that Pseudomonas aeruginosa and Staphylococcus caureus can show an affecting state in the positive test tube,and colonies can be found after observation.Conclusion:The application of membrane filtration method combined with centrifugal precipitation method to complete the inspection of antibiotic ointment can achieve satisfactory results.The method is simple to operate and has high feasibility.It is worthy of further promotion and application.
文摘Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lingam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum. The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control untreated and infested plants.
文摘Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was put into a heat treatment machine in pilot plan, and harmless condition in short time was examined. The results indicated, pathogenic indicator bacteria such as coliform bacteria, fecal coliform, Escherichia coli and salmonella were all 106 cfu/g dw at the beginning, died rapidly when cow dung temperature rose to above 50~C, and not detected at 54-68~C for 6-24 h heat treatment. Coliform bacteria and salmonella in heated cow dung were not detected by re-growth culture and enrichment culture examination. Moreover, it was hardly influenced on the fermentation ability of composting microbe, organic decomposition bacteria. During heat treatment, the mesophile decreased rapidly and the thermophile stabilized or increased, and the most of composting microbe were bacillus in cow dung by fluorescence microscope, this indicated that bacillus was dominator and composting microbe in composting process.
基金State Tobacco Monopoly Administration of China (110200201008)
文摘The possibility of employing antagonistic bacteria for the control of tobacco brown spot was studied. Approximately 136 strains of bacteria were isolated from phyllospheres of tobacco and 9 of these possessed high levels of antagonistic properties, They significantly reduced brown spot in detached tobacco leaves when artificially inoculated with Alternaria alternata. Culture filtrate of the most effective bacterial isolate which designated as Tpb88 was shown to be very efficient in inhibiting mycelial growth of A alternata in dual cultures. Culture filtrate of Tpb88 inhibited germination and germ tube elongation of A alternata. The results showed that the culture filtrate directly inhibited spore germination of A alternata, especially during the first hours of the paired cultivation. The rate of antagonistic activity of culture filtrate of Tpb88 depended on its concentration in the mixture. The greatest inhibition of spore germination was ob served at the highest concentration of filtrate （filtrate to fungal spores inocula mixed in proportion 1 ： 50）. These suggest that the hypothetic mechanism of Tpb88 against tobacco brown spot is to produce antagonistic substances.
文摘Introduction: The aim of this study was to improve the DISTER-UV and to perform microbiological quality control at the biomedical laboratory of the West African Polytechnic University from January 2022 to November 2022. Methodology: During this eleven-month prospective study, we set up a quality control device (QCD). For microbiological quality control, we performed different cultures of bacteria with different bacteriological and morphological characteristics at T0 (no sterilization) and at T30 (after 30 minutes of sterilization under DISTER-UV). Results: After the realization, the DCQ attached to the DISTER-UV1 allows to display of the UV wavelength present in the light box. This device also displays and alerts when the UV intensity emitted by the lamps is below 250 nm. During microbiological quality control, the cultures carried out at T30 and incubated for 24 hours did not reveal any bacterial colonies. This shows the bactericidal character of DISTER-UV-2. Conclusion: The improvement and the microbiological quality control allowed us to switch from DISTER-UV1 (without sensor) to DISTER-UV-2 (with sensor or DCQ). The biological control allowed us to affirm that the DISTER-UV-2 is bactericidal.
文摘Erwinia amylovora species were isolated from the blossoms, exudates, infected fruits, leaves and bent branches of diseased apple, pear and hawthorn trees, selected in the Chy, Osh and Jalal Abad regions. Biochemical and pathogenicity tests, alongside PCR analyses, were conducted to identify the local isolates of Erwinia amylovora. The alternative antagonistic microorganisms which combat bacterium E. amylovora were tested within in vitro and in vivo conditions. The results revealed the ability of Streptomyces antagonistic bacteria to decrease fire blight severity on pear and apple trees during the first stage of the fire blight disease in leaf tissues. Streptomyces strain C1-4 suppressed E. amylovora disease symptoms in the leaf tissues and excised apple and pear shoots. The incidence of fire blight on leaves was reduced by about 70% with two applications of bacterial antagonists. Further studies at different locations in Kyrgyzstan, using large scale application, would allow for stronger recommendations to be made, including studies and recommendations on their ability to prevent disease and to use them as main components in an integrated pest management program.
文摘Objective:The acceptability of herbal remedies for alleviating discomforts and ill-health has become very popular, on the account of the increasing cost of allopathic medicine for personal health maintenance.The observable non-adherence of herbalists to the established World Health Organization(WHO) / National Agency for Food and Drug Administration Control(NAFDAC) regulations for the quality control of herbal medicines is an issue for concern.In view of this,34 popular and widely consumed crude herbal remedies in southwestern,Nigeria were screened for compliance with standard limits for bacterial contamination,bacteria flora and their antibiotic susceptibility pattern.Methods:Isolates recovered from samples were identified using the cultural, morphological and biochemical characteristics.They were also tested for drug sensitivity using standard procedures. Results:A heavy bacteria load ranging from 3.00×10~3-9.58×10~5 CFU/ML and 1.20×10~5- 5.41×10~5 CFU/ML was observed for water and spirit extracted preparations respectively.The bacteria flora cum contaminants were:Staphylococcus aureus,Bacillus cereus,Bacillus subtilis,Pseudomonas aeruginosa, Micrococcus luteus,Lactobacillus plantarum,Klebsiella pneumoniae,Escherichia coli,streptococcus,Shigella, Neisseria,Arthrobacter,Kurthia and Clostridium species.All the isolates were multi-drug resistant(MDR) strains.Conclusion:The crude herbal preparations consumed in Nigeria failed to comply with the internationally recognized standards regarding bacteria load and flora.The presence of MDR pathogens is of greatest concern. It poses a great risk to consumers health and could be a source of introducing MDR organisms into the human population.There is the need for the enforcement of established guidelines to ensure the safety of these preparations.
