Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuo...Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuous light exposure.This study aimed to investigate the morphological and functional alterations in BAT under continuous light conditions and to identify traditional Chinese medicine compounds capable of reversing these changes.Methods:A metabolic disorder model was established by subjecting mice to continuous light exposure for 5 weeks.During this period,body weight,food intake,and body fat percentage were monitored.Serum levels of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)were measured to assess lipid metabolism.Histological changes in BAT were examined using H&E staining.The expression of the thermogenic marker uncoupling protein 1(UCP1)in BAT was determined by RT-qPCR and Western blot to evaluate thermogenic function.RNA sequencing(RNA-seq)was employed to identify differentially expressed genes(DEGs)involved in BAT whitening induced by prolonged continuous light exposure.DEGs were analyzed using the connectivity map(CMap)database to identify potential preventive and therapeutic compounds.The therapeutic efficacy of the selected compounds was subsequently evaluated using the above indicators,and key pathways were validated through western blot analysis.Results:After 5 weeks of continuous light exposure,mice exhibited increased body fat percentage and serum levels of TG,impaired mitochondrial function,reduced thermogenic capacity,and whitening of BAT.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses indicated that BAT whitening was primarily associated with the adenosine 5'-monophosphate-activated protein kinase(AMPK)signaling pathway,fatty acid metabolism,and circadian rhythm.Ten hub genes identified using Cytoscape were mainly related to AMPK signaling and heat shock proteins.In vivo experiments showed that cordycepin significantly attenuated the increase in body fat percentage caused by prolonged light exposure.This effect was mediated by activation of the AMPK/PGC-1α/UCP1 signaling pathway,which restored the multilocular morphology and thermogenic function of BAT.Conclusion:Cordycepin mitigates continuous light-induced BAT whitening and metabolic disturbances by activating the AMPK signaling pathway.展开更多
Studies have indicated that night lights interfere with the circadian rhythm in the human body and threaten human health. Our previous studies indicated that continuous light exposure severely damages the reproductive...Studies have indicated that night lights interfere with the circadian rhythm in the human body and threaten human health. Our previous studies indicated that continuous light exposure severely damages the reproductive endocrine system of female rats resembles polycystic ovary syndrome in women. In this study, we used the continuous measurement method to observe changes in the basal physiological indicators of female rats in an abnormal light exposure environment. Our study results indicated that in female rats: 1) the body temperature first continuously and gradually increased followed by a gradually decrease;2) the increase in body weight slowed down at the late stage of the experimental process;3) the random blood glucose level increased, and the fasting serum insulin level decreased;and 4) the serum C-reactive protein level increased. Our study investigated for the first time the correlation between the duration of continuous light exposure in female rats and the continuously measured basal physiological indicators and preliminarily discussed the effect of continuous light exposure on female basal metabolism and the possible inflammation mechanism. We propose that long-term continuous exposure to night lights in females severely damages their immune and metabolic systems.展开更多
The continuous white light(CWL)covering the visible and near-infrared(NIR)regions can be observed in various absorptive media excited by continuous-wave(CW)lasers.It is valuable to stimulate more efforts in unravellin...The continuous white light(CWL)covering the visible and near-infrared(NIR)regions can be observed in various absorptive media excited by continuous-wave(CW)lasers.It is valuable to stimulate more efforts in unravelling the involved photophysical processes and exploring its potential applications in diverse fields.Here,we proved that the enhanced thermal-field can boost the CWL emission.Using rare earth(RE)ions(Pr^(3+),Er^(3+)and Yb^(3+))as the photothermally active centers in Y_(2)SiO_(5)phosphor,we reveal that absorbing more excitation energy and isolating the heat conduction can lead to rapid thermal field accumulation inside the material,thereby significantly reducing the excitation threshold and enhancing white light emission.Our results might have important implications for the understanding of thermally enhanced radiation and may facilitate the CWL commercial application in night vision,bioimaging,and non-destructive detection.展开更多
Objective: to investigate the effect difference of intermittent and continuous treatment with Yinzhi Huang oral liquid in the treatment of neonatal jaundice (NNJ). Methods: a total of 200 children with NNJ from Januar...Objective: to investigate the effect difference of intermittent and continuous treatment with Yinzhi Huang oral liquid in the treatment of neonatal jaundice (NNJ). Methods: a total of 200 children with NNJ from January 2019 to December 2019 in our hospital were divided into two groups by odd-even method. The levels of bilirubin (BILirubin) before and after treatment, the outcome, the time of disease improvement and the incidence of phototherapy damage events in the two groups were compared. Results: there were significant differences in BILI level, outcome, improvement time and incidence of phototherapy damage events between the intermittent blue light treatment group and the continuous blue light treatment group (P < 0.05). Conclusion: in the aspect of blue light therapy for CHILDREN with NNJ, intermittent intervention can more significantly promote the decline of BILI level, accelerate the regression of yellow skin stain, accelerate the rehabilitation of children, and improve bilirubin level, which is worth promoting.展开更多
This research investigates the reliability of two measures intended to reduce moose-vehicle collisions(MVCs):continuous lighting and clearing/grubbing of roadway corridors.Individual analyses and a combined regression...This research investigates the reliability of two measures intended to reduce moose-vehicle collisions(MVCs):continuous lighting and clearing/grubbing of roadway corridors.Individual analyses and a combined regression analysis were conducted to measure the effects of several combinations of variables on MVC rates,including clearing and grubbing,continuous lighting,clearing without grubbing,moose population,precipitation,snowfall,and maximum snow depth.Nine corridor improvement projects were analyzed based on the variables present.In previous studies,it has been hypothesized that MVC rates are influenced by environmental conditions such as snowfall and daylight.The Alaska Department of Transportation and Public Facilities(DOT&PF)has performed many studies on MVCs along several corridors.Some corridors showed a significant drop in the number of MVCs after the installation of continuous lighting.The results show there is a consistent drop in MVCs after clearing and grubbing,with the exception of one corridor.The combined clearing/grubbing and continuous lighting projects also resulted in a consistent drop in MVCs.The projects with clearing and grubbing as a component had varying trends in MVCs,which may indicate that DOT&PF Maintenance and Operations performed clearing of re-vegetated areas,or that older growth is less of an attractant for moose.展开更多
文摘Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuous light exposure.This study aimed to investigate the morphological and functional alterations in BAT under continuous light conditions and to identify traditional Chinese medicine compounds capable of reversing these changes.Methods:A metabolic disorder model was established by subjecting mice to continuous light exposure for 5 weeks.During this period,body weight,food intake,and body fat percentage were monitored.Serum levels of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)were measured to assess lipid metabolism.Histological changes in BAT were examined using H&E staining.The expression of the thermogenic marker uncoupling protein 1(UCP1)in BAT was determined by RT-qPCR and Western blot to evaluate thermogenic function.RNA sequencing(RNA-seq)was employed to identify differentially expressed genes(DEGs)involved in BAT whitening induced by prolonged continuous light exposure.DEGs were analyzed using the connectivity map(CMap)database to identify potential preventive and therapeutic compounds.The therapeutic efficacy of the selected compounds was subsequently evaluated using the above indicators,and key pathways were validated through western blot analysis.Results:After 5 weeks of continuous light exposure,mice exhibited increased body fat percentage and serum levels of TG,impaired mitochondrial function,reduced thermogenic capacity,and whitening of BAT.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses indicated that BAT whitening was primarily associated with the adenosine 5'-monophosphate-activated protein kinase(AMPK)signaling pathway,fatty acid metabolism,and circadian rhythm.Ten hub genes identified using Cytoscape were mainly related to AMPK signaling and heat shock proteins.In vivo experiments showed that cordycepin significantly attenuated the increase in body fat percentage caused by prolonged light exposure.This effect was mediated by activation of the AMPK/PGC-1α/UCP1 signaling pathway,which restored the multilocular morphology and thermogenic function of BAT.Conclusion:Cordycepin mitigates continuous light-induced BAT whitening and metabolic disturbances by activating the AMPK signaling pathway.
文摘Studies have indicated that night lights interfere with the circadian rhythm in the human body and threaten human health. Our previous studies indicated that continuous light exposure severely damages the reproductive endocrine system of female rats resembles polycystic ovary syndrome in women. In this study, we used the continuous measurement method to observe changes in the basal physiological indicators of female rats in an abnormal light exposure environment. Our study results indicated that in female rats: 1) the body temperature first continuously and gradually increased followed by a gradually decrease;2) the increase in body weight slowed down at the late stage of the experimental process;3) the random blood glucose level increased, and the fasting serum insulin level decreased;and 4) the serum C-reactive protein level increased. Our study investigated for the first time the correlation between the duration of continuous light exposure in female rats and the continuously measured basal physiological indicators and preliminarily discussed the effect of continuous light exposure on female basal metabolism and the possible inflammation mechanism. We propose that long-term continuous exposure to night lights in females severely damages their immune and metabolic systems.
基金supported by the National Natural Science Foundation of China(12104336,U20A20211,62075152,12004274)Fundamental Research Program of Shanxi Province(20210302124162)。
文摘The continuous white light(CWL)covering the visible and near-infrared(NIR)regions can be observed in various absorptive media excited by continuous-wave(CW)lasers.It is valuable to stimulate more efforts in unravelling the involved photophysical processes and exploring its potential applications in diverse fields.Here,we proved that the enhanced thermal-field can boost the CWL emission.Using rare earth(RE)ions(Pr^(3+),Er^(3+)and Yb^(3+))as the photothermally active centers in Y_(2)SiO_(5)phosphor,we reveal that absorbing more excitation energy and isolating the heat conduction can lead to rapid thermal field accumulation inside the material,thereby significantly reducing the excitation threshold and enhancing white light emission.Our results might have important implications for the understanding of thermally enhanced radiation and may facilitate the CWL commercial application in night vision,bioimaging,and non-destructive detection.
文摘Objective: to investigate the effect difference of intermittent and continuous treatment with Yinzhi Huang oral liquid in the treatment of neonatal jaundice (NNJ). Methods: a total of 200 children with NNJ from January 2019 to December 2019 in our hospital were divided into two groups by odd-even method. The levels of bilirubin (BILirubin) before and after treatment, the outcome, the time of disease improvement and the incidence of phototherapy damage events in the two groups were compared. Results: there were significant differences in BILI level, outcome, improvement time and incidence of phototherapy damage events between the intermittent blue light treatment group and the continuous blue light treatment group (P < 0.05). Conclusion: in the aspect of blue light therapy for CHILDREN with NNJ, intermittent intervention can more significantly promote the decline of BILI level, accelerate the regression of yellow skin stain, accelerate the rehabilitation of children, and improve bilirubin level, which is worth promoting.
基金the Alaska Department of Transportation and Public Facilities(DOT&PF)and the Federal Highway Administration(FHWA)for providing funding for this project under grant number Z586520000/RSA 2558049,and for the cooperation of Alaska Department of Fish and Game(ADF&G).
文摘This research investigates the reliability of two measures intended to reduce moose-vehicle collisions(MVCs):continuous lighting and clearing/grubbing of roadway corridors.Individual analyses and a combined regression analysis were conducted to measure the effects of several combinations of variables on MVC rates,including clearing and grubbing,continuous lighting,clearing without grubbing,moose population,precipitation,snowfall,and maximum snow depth.Nine corridor improvement projects were analyzed based on the variables present.In previous studies,it has been hypothesized that MVC rates are influenced by environmental conditions such as snowfall and daylight.The Alaska Department of Transportation and Public Facilities(DOT&PF)has performed many studies on MVCs along several corridors.Some corridors showed a significant drop in the number of MVCs after the installation of continuous lighting.The results show there is a consistent drop in MVCs after clearing and grubbing,with the exception of one corridor.The combined clearing/grubbing and continuous lighting projects also resulted in a consistent drop in MVCs.The projects with clearing and grubbing as a component had varying trends in MVCs,which may indicate that DOT&PF Maintenance and Operations performed clearing of re-vegetated areas,or that older growth is less of an attractant for moose.
