[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous ...[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.展开更多
First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provi...First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.展开更多
Panax ginseng,a perennial herbaceous plant and a representative of the Panax genus,is renowned for its exceptional medicinal value and economic benefits,often referred to as the“King of Herbs.”With the increasingmar...Panax ginseng,a perennial herbaceous plant and a representative of the Panax genus,is renowned for its exceptional medicinal value and economic benefits,often referred to as the“King of Herbs.”With the increasingmarket demand and the limited availability of suitable cultivation land,the issue of continuous cropping obstacles for P.ginseng has become increasingly prominent,directly hindering the sustainable development of the ginseng industry.This article summarizes the concept and hazards of continuous cropping obstacles and,drawing on the latest research,provides an in-depth analysis of the causes and responsemechanisms.This work aims to establish a solid foundation for future research into the mechanisms of continuous cropping obstacles in P.ginseng.展开更多
Due to the increasing global population and limited land resources, continuous cropping has become common. However, after a few years of continuous cropping, obstacles often arise that cause soil degeneration, decreas...Due to the increasing global population and limited land resources, continuous cropping has become common. However, after a few years of continuous cropping, obstacles often arise that cause soil degeneration, decreased crop yield and quality, and increased disease incidence, resulting in significant economic losses. It is essential to understand the causes and mitigation mechanisms of continuous cropping obstacles(CCOs) and then develop appropriate methods to overcome them. This review systematically summarizes the causes and mitigation measures of soil degradation in continuous cropping through a meta-analysis. It was concluded that not all continuous cropping systems are prone to CCOs. Therefore, it is necessary to grasp the principles governing the occurrence of diseases caused by soilborne pathogens in different cropping systems, consider plant–soil-organisms interactions as a system, scientifically regulate the physical and chemical properties of soils from a systems perspective, and then regulate the structure of microbial food webs in the soil to achieve a reduction in diseases caused by soilborne pathogens and increase crop yield ultimately. This review provides reference data and guidance for addressing this fundamental problem.展开更多
Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic ...Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.展开更多
Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reducti...Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.展开更多
Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potent...Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.展开更多
At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degr...At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.展开更多
Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequa...Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequately addressed. The objective of the present study was to assess the effects on soil microbial community and yields of waxy maize（Zea mays L.） intercropped with or without residue retention in a continuous broccoli（Brassica oleracea L.） cropping system, i.e., relay intercropping of broccoli and waxy maize（B/M-B）, relay intercropping of broccoli and waxy maize with residue retention（B/MR-B）, and broccoli monoculture（B-B）. The biomass yields of spring and autumn vegetables in B/MR-B were 16.3%–32.5% and 30.1%–46.1% higher than those of B-B,respectively. Autumn vegetable economic yields of B/MR-B were 28.2%–40.3% higher than B-B. The average well color development followed the order： B/MR-B ＆gt; B/M-B ＆gt; B-B. The Shannon index, Simpson index, and Mc Intosh index were higher in B/MR-B than under monoculture. A principal component analysis showed that microbial communities of B/MR-B soils differed from those of B/M-B and B-B soils. Carbon（C） sources utilized by the rhizosphere microorganisms were mainly carbohydrates, carboxylic acids, amino acids, and polymers; however, the C sources for the soil microbial community differed between intercropping and monoculture. The communities from B/MR-B preferred amino acids and polymers. Available nitrogen（N）, potassium（K）, and phosphorus（P） had an obvious impact on soil microbial community. Additionally, the C source utilization by microorganisms was significantly affected by p H and available K and P. Cropping system diversification through relay intercropping and residue retention effectively improved the functional diversity of the soil microbial communities and increased the yields of vegetables.展开更多
Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study wa...Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.展开更多
To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of fore...To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of forest land, increase the in-come of forest farmers and protect the ecological environment, the cultivation tech-niques of high-quality P. notoginseng seedlings from Wenshan, Yunnan under four kinds of forests （walnut forest, China fir forest, grape forest and kiwi forest） were in-vestigated in this study. The results showed that the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng_under walnut forest were higher than those under the other three kinds of forests; the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng under China fir forest were higher than those under grape forest and kiwi forest; and the crown di-ameter and survival rate under grape forest were higher, and the height growth and tuber weight under grape forest were lower than those under kiwi forest. Walnut is a broad-leaved deciduous tree species, so large-scale cultivation of P. notoginseng_should be conducted under broadleaf deciduous forest with canopy density around 0.8, taking advantage of the cool environment and rich humus layer under forest. This cultivation technology could save labor, shade, fertilizer and other costs, and accord with the ecological habit and the growth rules of P. notoginseng, thus im-proving yield and achieving high economic benefit.展开更多
Objective To study the effect of autotoxicity and the co-effect of autotoxicity and soil microbes from continuous cropping soil on Angelica sinensis growth, root yield and content of essential oils, and soilmicrobalpo...Objective To study the effect of autotoxicity and the co-effect of autotoxicity and soil microbes from continuous cropping soil on Angelica sinensis growth, root yield and content of essential oils, and soilmicrobalpopulation. Methods The pot experiments were conducted upon A. sinensis seedlings in continuous cropping soil. At the different growth stages, we determined the seedlings in growth parameters, root yield, content of essential oils, ethanol extract, and cultivable microbial populations in rhizosphere soil. Results A. sinensis seedlings were significantly inhibited in growth, root yield and quality. Compared with the control, the composition and structure of soil microbes were changed and the diversity indexes of bacteria functional groups were reduced in rhizosphere soil ofA. sinensis. A. sinensiscropping problems were more seriously after the treatment with combination of autotoxicity and soil microbes than with autotoxicity alone. Conclusion The autotoxicityand soil microbes from continuous cropping soil of A. sinensis could cause the continuous cropping obstacle together.展开更多
Panax notoginseng(Araliaceae)is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years.In recent years?.notoginseng has faced serious continuous-croppi...Panax notoginseng(Araliaceae)is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years.In recent years?.notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation.In this study,we aim to explore the allelochemicals of P.notoginseng and their interactions with various plants and rhizosphere microor-ganisms.The chemical constituents of the soil cultivated with 3-year-old P.notoginseng were studied by column chromatography,spectroscopic and GC-MS analyses.We identified 13 volatile components and isolated six triterpenes(1-4,6-7)and one anthraquinone(5).Compounds 1-7 were tested for their effects on seed germination and root elongation in P.notoginseng,corn,wheat,turnip,water spinach and Arabidopsis thaliana.We also examined the effect of compounds 1-7on the growth of ten rhizosphere microorganisms of P.notoginseng.At a concentration of 1.0 ug mL',compounds 3 and 5-7 caused the death of P.notoginseng root cells and compounds 2,6 and 7 induced the death of root cells of A.thaliana.Compounds 1-5and 7 inhibited elongation of A.thaliana root tip cells at a concentration of 10.0μg mL^-1.Moreover,at a concentration of 0.1 mg mL^-1,compounds 3,4,6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P.notoginseng.These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P.notoginseng.展开更多
[Objective] The aim was to identify changes in a nematode community in response to crop rotation and to determine the appropriate catch crop for a greenhouse. [Method] The experiment was carried out in a typical 6-yea...[Objective] The aim was to identify changes in a nematode community in response to crop rotation and to determine the appropriate catch crop for a greenhouse. [Method] The experiment was carried out in a typical 6-year-old greenhouse,in which cucumber crops were cultivated twice each year(in spring and autumn),and catch crops were planted in summer. The total number of nematodes was counted and nematode community indices were calculated after collecting soil samples in different stages. [Result] Total nematode abundance was decreased in the soils of catch crop in contrast with former crops(cucumber crops). The abundance of the nematode community was reduced in the treatment of crop rotation compared to the soils of catch crop. In addition, the number of nematode taxa was significantly reduced by the treatment of crown daisy compared to the treatments of following crops. Crop rotation regulated the functional composition of the nematode community by increasing the omnivores-predators functional group and decreasing the relative abundance of root herbivores. [Conclusion] These results indicate that crop rotation affects the nematode community in abundance, diversity and functional composition of the nematode community and crown daisy can be served as the most appropriate catch crop in the greenhouse.展开更多
Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiologi...Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiological and photosynthetic characteristics of Z.simulans to provide a theoretical reference for continuous Z.simulans crop cultivation.A bioorganic fertilizer was used to treat seedlings growing in 25-year-old continuous cropping soil.Five fertilizer treatments were applied.The impacts of the treatments on the activity of defense enzyme and photosynthetic parameters of Z.simulans leaves were determined.The different concentrations of bioorganic fertilizer reduced to varying degrees the malondialdehyde(MDA)content and intercellular CO_(2) concentration(Ci),and increased the activity of peroxidase(POD),superoxide dismutase(SOD),and ascorbate peroxidase(APX),as well as the chlorophyll content,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of Z.simulans leaves.The results showed that most significant increases or decreases were achieved with 100 g/L bioorganic fertilizer(Y2).Thus,the application of bioorganic fertilizer at a rate of 100 g/L can significantly improve the activity of relevant defense enzymes and photosynthetic parameters of Z.simulans,and reduce the MDA content,enhancing the stress resistance of the plants,promoting their growth and addressing,to some extent,obstacles associated with continuous cultivation.展开更多
Root-knot nematodes(Meloidogyne spp.)have garnered significant attention from researchers owing to the substantial damage they cause to crops and their worldwide distribution.However,controlling these nematodes is cha...Root-knot nematodes(Meloidogyne spp.)have garnered significant attention from researchers owing to the substantial damage they cause to crops and their worldwide distribution.However,controlling these nematodes is challenging because a limited number of chemical pesticides and biocontrol agents are effective against them.Here,we demonstrate that pepper rotation markedly reduces Meloidogyne incognita infection in cucumber and diminishes the presence of p-hydroxybenzoic acid in the soil,a compound known to exacerbate M.incognita infection.Pepper rotation also restructures the rhizobacterial community,leading to the colonization of the cucumber rhizosphere by two Pseudarthrobacter oxydans strains(RH60 and RH97),facilitated by enrichment of palmitic acid in pepper root exudates.Both strains exhibit high nematocidal activity against M.incognita and have the ability to biosynthesize indoleacetic acid and biodegrade p-hydroxybenzoic acid.RH60 and RH97 also induce systemic resistance in cucumber plants and promote their growth.These data suggest that the pepper root exudate palmitic acid alleviates M.incognita infection by recruiting beneficial P.oxydans to the cucumber rhizosphere.Our analyses identify a novel chemical component in root exudates and reveal its pivotal role in crop rotation for disease control,providing intriguing insights into the keystone function of root exudates in plant protection against root-knot nematode infection.展开更多
基金Supported by Agricultural Science and Technology Innovation Fund of Hunan Province(XCNZ[2021]No.15)Loudi Science and Technology Innovation Program(LKF[2022]29)+1 种基金Applied Characteristic Discipline Construction Project of Hunan Province:Plant ProtectionPostgraduate Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2022Y12).
文摘[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.
文摘First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.
基金funded byNational Natural Science Foundation of China(82003902),Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2021B014)Fundamental Research Funds for Central Public Welfare Research Institutes(ZZXT202008/ZZ14-YQ-046)the National Key R&D Program of China(2023YFC3400585,2020YFA0908000).
文摘Panax ginseng,a perennial herbaceous plant and a representative of the Panax genus,is renowned for its exceptional medicinal value and economic benefits,often referred to as the“King of Herbs.”With the increasingmarket demand and the limited availability of suitable cultivation land,the issue of continuous cropping obstacles for P.ginseng has become increasingly prominent,directly hindering the sustainable development of the ginseng industry.This article summarizes the concept and hazards of continuous cropping obstacles and,drawing on the latest research,provides an in-depth analysis of the causes and responsemechanisms.This work aims to establish a solid foundation for future research into the mechanisms of continuous cropping obstacles in P.ginseng.
基金funded by the National Key Research and Development Program of China (2022YFD1901500, 2022YFD1901501, and 2023YFD1700203)the National Natural Science Foundation of China (32372810 and 32102469)+1 种基金the Science and Technology Major Project of Yunnan Province of China (202102AE090053)the Science and Technology Training Program (202305AF150055)。
文摘Due to the increasing global population and limited land resources, continuous cropping has become common. However, after a few years of continuous cropping, obstacles often arise that cause soil degeneration, decreased crop yield and quality, and increased disease incidence, resulting in significant economic losses. It is essential to understand the causes and mitigation mechanisms of continuous cropping obstacles(CCOs) and then develop appropriate methods to overcome them. This review systematically summarizes the causes and mitigation measures of soil degradation in continuous cropping through a meta-analysis. It was concluded that not all continuous cropping systems are prone to CCOs. Therefore, it is necessary to grasp the principles governing the occurrence of diseases caused by soilborne pathogens in different cropping systems, consider plant–soil-organisms interactions as a system, scientifically regulate the physical and chemical properties of soils from a systems perspective, and then regulate the structure of microbial food webs in the soil to achieve a reduction in diseases caused by soilborne pathogens and increase crop yield ultimately. This review provides reference data and guidance for addressing this fundamental problem.
基金supported by the National Key Research and Development Program of China(2023YFD1700203and 2022YFD1901501)the Tianchi Talent Introduction Program of Xinjiang Autonomous Region,China(2023-“2+5”)the Tingzhou Talent Introduction Program of Changji Autonomous Region,China(2023)。
文摘Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.
基金Supported by Key Project of Yunnan Provincial Science and Technology Plan(202202AE090015)Scientific Research Fund of Yunnan Education Department(2024Y742+3 种基金2023Y0863)National Natural Science Foundation of China(42067009)2023 Undergraduate Innovation and Entrepreneurship Training Program of Yunnan Education Department(S202311393044S202311393061).
文摘Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.
基金Supported by National Natural Science Foundation of China(42067009)Scientific Research Project of Yunnan Provincial Department of Education(2024Y742,2023Y0863)+2 种基金Project of Kunming Municipal Commission of Development and Reform(Kunming Spring City Industrial Technology Leading Talents)Science and Technology Major Project of Yunnan Province Science and Technology Department(202202AE090015-02)Yunnan Students’innovation and entrepreneurship training program(S202311393044,S202311393061)。
文摘Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.
基金Supported by Scientific Research Fund of Yunnan Education Department(2024Y742,2023Y0863)National Natural Science Foundation of China(42067009)+1 种基金College Students'Innovative Training Plan Program of Yunnan Education Department in 2023(S202311393044,S202311393061)Key Project of Science and Technology Program of Yunnan Province(202202AE090015).
文摘At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.
基金financially supported by the Special Research Funding for Public Benefit Industries (Agriculture) of China (No. 201503121)the Key Projects in the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period, China (No. 2012BAD14B07)
文摘Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequately addressed. The objective of the present study was to assess the effects on soil microbial community and yields of waxy maize（Zea mays L.） intercropped with or without residue retention in a continuous broccoli（Brassica oleracea L.） cropping system, i.e., relay intercropping of broccoli and waxy maize（B/M-B）, relay intercropping of broccoli and waxy maize with residue retention（B/MR-B）, and broccoli monoculture（B-B）. The biomass yields of spring and autumn vegetables in B/MR-B were 16.3%–32.5% and 30.1%–46.1% higher than those of B-B,respectively. Autumn vegetable economic yields of B/MR-B were 28.2%–40.3% higher than B-B. The average well color development followed the order： B/MR-B ＆gt; B/M-B ＆gt; B-B. The Shannon index, Simpson index, and Mc Intosh index were higher in B/MR-B than under monoculture. A principal component analysis showed that microbial communities of B/MR-B soils differed from those of B/M-B and B-B soils. Carbon（C） sources utilized by the rhizosphere microorganisms were mainly carbohydrates, carboxylic acids, amino acids, and polymers; however, the C sources for the soil microbial community differed between intercropping and monoculture. The communities from B/MR-B preferred amino acids and polymers. Available nitrogen（N）, potassium（K）, and phosphorus（P） had an obvious impact on soil microbial community. Additionally, the C source utilization by microorganisms was significantly affected by p H and available K and P. Cropping system diversification through relay intercropping and residue retention effectively improved the functional diversity of the soil microbial communities and increased the yields of vegetables.
基金supported by the National Key R&D Program of China(2018YFD1000902)the Natural Science Foundation of Shandong Province(ZR2021QC163).
文摘Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.
基金Supported by Central Financial Forestry Science and Technology Extension Project of China([2016]XT001)Science and Technology Development Project of Hunan Province(S2014F209021)~~
文摘To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of forest land, increase the in-come of forest farmers and protect the ecological environment, the cultivation tech-niques of high-quality P. notoginseng seedlings from Wenshan, Yunnan under four kinds of forests （walnut forest, China fir forest, grape forest and kiwi forest） were in-vestigated in this study. The results showed that the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng_under walnut forest were higher than those under the other three kinds of forests; the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng under China fir forest were higher than those under grape forest and kiwi forest; and the crown di-ameter and survival rate under grape forest were higher, and the height growth and tuber weight under grape forest were lower than those under kiwi forest. Walnut is a broad-leaved deciduous tree species, so large-scale cultivation of P. notoginseng_should be conducted under broadleaf deciduous forest with canopy density around 0.8, taking advantage of the cool environment and rich humus layer under forest. This cultivation technology could save labor, shade, fertilizer and other costs, and accord with the ecological habit and the growth rules of P. notoginseng, thus im-proving yield and achieving high economic benefit.
基金Natural Science Foundation of China(31060182)Agricultural Biotechnology Research and Application Development of Gansu province(GNSW-2010-18)
文摘Objective To study the effect of autotoxicity and the co-effect of autotoxicity and soil microbes from continuous cropping soil on Angelica sinensis growth, root yield and content of essential oils, and soilmicrobalpopulation. Methods The pot experiments were conducted upon A. sinensis seedlings in continuous cropping soil. At the different growth stages, we determined the seedlings in growth parameters, root yield, content of essential oils, ethanol extract, and cultivable microbial populations in rhizosphere soil. Results A. sinensis seedlings were significantly inhibited in growth, root yield and quality. Compared with the control, the composition and structure of soil microbes were changed and the diversity indexes of bacteria functional groups were reduced in rhizosphere soil ofA. sinensis. A. sinensiscropping problems were more seriously after the treatment with combination of autotoxicity and soil microbes than with autotoxicity alone. Conclusion The autotoxicityand soil microbes from continuous cropping soil of A. sinensis could cause the continuous cropping obstacle together.
基金Acknowledgments The authors are grateful to the members of the Analytical Group in State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,for measuring all the spectra.We also appreciate Prof.Wei-Qi Li,Dr.Yan-Xia Jia and Dr.Xing Huang for providing materials and technical support on plant bioassays.This work is supported by the Science and Technology Planning Project(2013FC008)the Major Science and Technique Programs(2016ZF001-001)Yunnan Province,China,and Yung-Chi Cheng academician workstation of Yunnan provincial academy of science and technology(2015IC017).
文摘Panax notoginseng(Araliaceae)is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years.In recent years?.notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation.In this study,we aim to explore the allelochemicals of P.notoginseng and their interactions with various plants and rhizosphere microor-ganisms.The chemical constituents of the soil cultivated with 3-year-old P.notoginseng were studied by column chromatography,spectroscopic and GC-MS analyses.We identified 13 volatile components and isolated six triterpenes(1-4,6-7)and one anthraquinone(5).Compounds 1-7 were tested for their effects on seed germination and root elongation in P.notoginseng,corn,wheat,turnip,water spinach and Arabidopsis thaliana.We also examined the effect of compounds 1-7on the growth of ten rhizosphere microorganisms of P.notoginseng.At a concentration of 1.0 ug mL',compounds 3 and 5-7 caused the death of P.notoginseng root cells and compounds 2,6 and 7 induced the death of root cells of A.thaliana.Compounds 1-5and 7 inhibited elongation of A.thaliana root tip cells at a concentration of 10.0μg mL^-1.Moreover,at a concentration of 0.1 mg mL^-1,compounds 3,4,6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P.notoginseng.These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P.notoginseng.
基金supported by a Key Grant from the Earmarked Fund for Beijing Leaf Vegetables Innovation Team of Modern Agro-industry Technology Research System (blvt-08)the Natural Science Foundation of Beijing (Project 6972014)the National Science Foundation of China (Grant No. 30972034)
文摘[Objective] The aim was to identify changes in a nematode community in response to crop rotation and to determine the appropriate catch crop for a greenhouse. [Method] The experiment was carried out in a typical 6-year-old greenhouse,in which cucumber crops were cultivated twice each year(in spring and autumn),and catch crops were planted in summer. The total number of nematodes was counted and nematode community indices were calculated after collecting soil samples in different stages. [Result] Total nematode abundance was decreased in the soils of catch crop in contrast with former crops(cucumber crops). The abundance of the nematode community was reduced in the treatment of crop rotation compared to the soils of catch crop. In addition, the number of nematode taxa was significantly reduced by the treatment of crown daisy compared to the treatments of following crops. Crop rotation regulated the functional composition of the nematode community by increasing the omnivores-predators functional group and decreasing the relative abundance of root herbivores. [Conclusion] These results indicate that crop rotation affects the nematode community in abundance, diversity and functional composition of the nematode community and crown daisy can be served as the most appropriate catch crop in the greenhouse.
基金This study was supported by the Central Committee for guiding the local science and technology development sub-project“Study on the Structural Adjustment and Optimization Research and Platform Construction of the Characteristic Economic Forest and Pepper in Longnan”,and thanks for the International Science Editing(http://www.Internationalscienceediting.com)for editing this manuscript.
文摘Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiological and photosynthetic characteristics of Z.simulans to provide a theoretical reference for continuous Z.simulans crop cultivation.A bioorganic fertilizer was used to treat seedlings growing in 25-year-old continuous cropping soil.Five fertilizer treatments were applied.The impacts of the treatments on the activity of defense enzyme and photosynthetic parameters of Z.simulans leaves were determined.The different concentrations of bioorganic fertilizer reduced to varying degrees the malondialdehyde(MDA)content and intercellular CO_(2) concentration(Ci),and increased the activity of peroxidase(POD),superoxide dismutase(SOD),and ascorbate peroxidase(APX),as well as the chlorophyll content,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of Z.simulans leaves.The results showed that most significant increases or decreases were achieved with 100 g/L bioorganic fertilizer(Y2).Thus,the application of bioorganic fertilizer at a rate of 100 g/L can significantly improve the activity of relevant defense enzymes and photosynthetic parameters of Z.simulans,and reduce the MDA content,enhancing the stress resistance of the plants,promoting their growth and addressing,to some extent,obstacles associated with continuous cultivation.
基金supported by the National Key Research and Development Program of China(2023YFD1400400,2017YFD0200603)the National Natural Science Foundation of China(32272493)the Key Research and Development Program of Jiangxi Province(20181ACF60018).
文摘Root-knot nematodes(Meloidogyne spp.)have garnered significant attention from researchers owing to the substantial damage they cause to crops and their worldwide distribution.However,controlling these nematodes is challenging because a limited number of chemical pesticides and biocontrol agents are effective against them.Here,we demonstrate that pepper rotation markedly reduces Meloidogyne incognita infection in cucumber and diminishes the presence of p-hydroxybenzoic acid in the soil,a compound known to exacerbate M.incognita infection.Pepper rotation also restructures the rhizobacterial community,leading to the colonization of the cucumber rhizosphere by two Pseudarthrobacter oxydans strains(RH60 and RH97),facilitated by enrichment of palmitic acid in pepper root exudates.Both strains exhibit high nematocidal activity against M.incognita and have the ability to biosynthesize indoleacetic acid and biodegrade p-hydroxybenzoic acid.RH60 and RH97 also induce systemic resistance in cucumber plants and promote their growth.These data suggest that the pepper root exudate palmitic acid alleviates M.incognita infection by recruiting beneficial P.oxydans to the cucumber rhizosphere.Our analyses identify a novel chemical component in root exudates and reveal its pivotal role in crop rotation for disease control,providing intriguing insights into the keystone function of root exudates in plant protection against root-knot nematode infection.
