Dynamic control is essential to guarantee the stable performance of continuous chromatography.AutoMAb dynamic control strategy has been developed to ensure a consistent protein load in twincolumn CaptureSMB continuous...Dynamic control is essential to guarantee the stable performance of continuous chromatography.AutoMAb dynamic control strategy has been developed to ensure a consistent protein load in twincolumn CaptureSMB continuous capture by integrating the UV signal of breakthrough.In this study,the process risk of CaptureSMB continuous capture under AutoMAb control towards the feedstock variations was assessed by a mechanistic model developed by us.The effects of target protein and impurities under the variation range of±10 mAU·min^(-1) on load amount,protein loss,process productivity,and resin capacity utilization were investigated.The results showed that the CaptureSMB process could be successfully controlled by AutoMAb towards increased or slightly decreased concentration of feedstock.However,the load process would be out of control with drastically decreased target protein or impurities,and the decreased impurities would lead to protein loss.It was found that AutoMAb control would cause 44.7%non-operational areas and 18.3%protein loss areas in the variation range of±10 mAU·min^(-1).To improve the stability of the CaptureSMB process,a modified AutoMAb control that would stop the load procedure when the absolute value of the integral area reached the preset value,was proposed to reduce the risk of protein loss and the non-operational area.展开更多
A well designed continuous annular chromatograph （CAC） column was set to stndy the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a ...A well designed continuous annular chromatograph （CAC） column was set to stndy the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a width of 15 mm. The weak basic resin （Diaion WA21J）, the boric acid and water were used as the absorbent, the feed and the eluent respectively. The effects of CAC rotating speed, concentration of boric acid and flow ratio of eluent to feed on the enrichment of 10B were investigated. The concentration of boron isotope was determined by a Thermal Elemental X7 ICP-MS （Thermo Electron Co., USA）. It is shown from the experimental results that 10B and 11B can be separated effectively by this CAC column. By fitting the experimental elution profiles based on reliable mathematic models and software the mass transfer coefficient k and adsorp- tion equilibrium constant K values of 10B and 11B in CAC column were estimated. It is clear from this study that the CAC has practical continuous operational capabilities in comparison with fixed batch chromatography and is an effective technology for seoarating boron isotones.展开更多
基金supported by the Zhejiang Key Science and Technology Project(2023C03116)National Natural Science Foundation of China(22078286)National Key Research and Development Program of China(2021YFE0113300).
文摘Dynamic control is essential to guarantee the stable performance of continuous chromatography.AutoMAb dynamic control strategy has been developed to ensure a consistent protein load in twincolumn CaptureSMB continuous capture by integrating the UV signal of breakthrough.In this study,the process risk of CaptureSMB continuous capture under AutoMAb control towards the feedstock variations was assessed by a mechanistic model developed by us.The effects of target protein and impurities under the variation range of±10 mAU·min^(-1) on load amount,protein loss,process productivity,and resin capacity utilization were investigated.The results showed that the CaptureSMB process could be successfully controlled by AutoMAb towards increased or slightly decreased concentration of feedstock.However,the load process would be out of control with drastically decreased target protein or impurities,and the decreased impurities would lead to protein loss.It was found that AutoMAb control would cause 44.7%non-operational areas and 18.3%protein loss areas in the variation range of±10 mAU·min^(-1).To improve the stability of the CaptureSMB process,a modified AutoMAb control that would stop the load procedure when the absolute value of the integral area reached the preset value,was proposed to reduce the risk of protein loss and the non-operational area.
基金supported by the Scientific and Technological Innovation Capacity Promotion Plan Project of Qinghai Province of China(2010-J-749)
文摘A well designed continuous annular chromatograph （CAC） column was set to stndy the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a width of 15 mm. The weak basic resin （Diaion WA21J）, the boric acid and water were used as the absorbent, the feed and the eluent respectively. The effects of CAC rotating speed, concentration of boric acid and flow ratio of eluent to feed on the enrichment of 10B were investigated. The concentration of boron isotope was determined by a Thermal Elemental X7 ICP-MS （Thermo Electron Co., USA）. It is shown from the experimental results that 10B and 11B can be separated effectively by this CAC column. By fitting the experimental elution profiles based on reliable mathematic models and software the mass transfer coefficient k and adsorp- tion equilibrium constant K values of 10B and 11B in CAC column were estimated. It is clear from this study that the CAC has practical continuous operational capabilities in comparison with fixed batch chromatography and is an effective technology for seoarating boron isotones.
