Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene I （MEIG 1） plays an essential role in the regulation of spermiogenesis. To explore potential mech...Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene I （MEIG 1） plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIGI＇s action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane occupation and recognition nexus repeat containing 3 （MORN3）. MORN3 mRNA is only abundant in mouse testis. In the testis, Morn3 mRNA is highly expressed in the spermiogenesis stage. Specific anti-MORN3 polyclonal antibody was generated against N-terminus of the full-length MORN3 protein, and MORN3 expression and localization was examined in vitro and in vivo. In transfected Chinese hamster ovary cells, the antibody specifically crossed-reacted the full-length MORN3 protein, and immunofluorescence staining revealed that MORN3 was localized throughout the cytoplasm. Among multiple mouse tissues, about 25 kDa protein, was identified only in the testis. The protein was highly expressed after day 20 of birth. Immunofluorescence staining on mixed testicular cells isolated from adult wild-type mice demonstrated that MORN3 was expressed in the acrosome in germ cells throughout spermiogenesis. The protein was also present in the manchette of elongating spermatids. The total MORN3 expression and acrosome localization were not changed in the Meig/-deficient mice. However, its expression in manchette was dramatically reduced in the mutant mice. Our studies suggest that MORN3 is another regulator for spermatogenesis, probably together with MEIG1.展开更多
A method is put forward to realize the recognition and guiding of initial welding position. The weld seams are marked with black lines, which simplify the computational complexity of image processing greatly. A two-ti...A method is put forward to realize the recognition and guiding of initial welding position. The weld seams are marked with black lines, which simplify the computational complexity of image processing greatly. A two-time template matching method has been advanced to search for the target point, which is simple and has higher calculation speed. According to the depth computing principle with the special point matching using binocular stereovision, the initial welding position can be confirmed by calculating the middle point of the perpendicular line of two radials in the space. Taking the welding of propellant fuel container for example, good results are obtained with the algorithms. Finally, similar method for terminating welding position is also advanced.展开更多
文摘Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene I （MEIG 1） plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIGI＇s action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane occupation and recognition nexus repeat containing 3 （MORN3）. MORN3 mRNA is only abundant in mouse testis. In the testis, Morn3 mRNA is highly expressed in the spermiogenesis stage. Specific anti-MORN3 polyclonal antibody was generated against N-terminus of the full-length MORN3 protein, and MORN3 expression and localization was examined in vitro and in vivo. In transfected Chinese hamster ovary cells, the antibody specifically crossed-reacted the full-length MORN3 protein, and immunofluorescence staining revealed that MORN3 was localized throughout the cytoplasm. Among multiple mouse tissues, about 25 kDa protein, was identified only in the testis. The protein was highly expressed after day 20 of birth. Immunofluorescence staining on mixed testicular cells isolated from adult wild-type mice demonstrated that MORN3 was expressed in the acrosome in germ cells throughout spermiogenesis. The protein was also present in the manchette of elongating spermatids. The total MORN3 expression and acrosome localization were not changed in the Meig/-deficient mice. However, its expression in manchette was dramatically reduced in the mutant mice. Our studies suggest that MORN3 is another regulator for spermatogenesis, probably together with MEIG1.
基金This project is supported by National Natural Science Foundation of China(No.60474036) and Shanghai Municipal Science and Technology CommitteeFoundation, China (No.021111116).
文摘A method is put forward to realize the recognition and guiding of initial welding position. The weld seams are marked with black lines, which simplify the computational complexity of image processing greatly. A two-time template matching method has been advanced to search for the target point, which is simple and has higher calculation speed. According to the depth computing principle with the special point matching using binocular stereovision, the initial welding position can be confirmed by calculating the middle point of the perpendicular line of two radials in the space. Taking the welding of propellant fuel container for example, good results are obtained with the algorithms. Finally, similar method for terminating welding position is also advanced.
