The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure ...The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure variations,potentially causing catastrophic damage to the container.Current studies mainly focus on non-deforming projectiles,such as fragments,with limited exploration of shaped charge jets.In this paper,a uniquely experimental system was designed to record cavity profiles in behind-armor liquid-filled containers subjected to shaped charge jet impacts.The impact process was then numerically reproduced using the explicit simulation program ANSYS LS-DYNA with the Structured Arbitrary Lagrangian-Eulerian(S-ALE)solver.The formation mechanism,along with the dimensional and shape evolution of the cavity was investigated.Additionally,the influence of the impact kinetic energy of the jet on the cavity characteristics was analyzed.The findings reveal that the cavity profile exhibits a conical shape,primarily driven by direct jet impact and inertial effects.The expansion rates of both cavity length and maximum radius increase with jet impact kinetic energy.When the impact kinetic energy is reduced to 28.2 kJ or below,the length-to-diameter ratio of the cavity ultimately stabilizes at approximately 7.展开更多
The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the bloo...The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.展开更多
The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The ...The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The idea of using shipping containers as a building component is by no means new in the Accra Metropolis as most shipping containers are re-constructed architecturally and used for temporary accommodation needs like storage,make-shift shops,emergency shelters and site offices.The concept of using these shipping containers as modular building components in architecture however,is still foreign to building practitioners and the nation at large.This research paper set out to use the containers not for luxury apartments but to harness the merits of availability,low-cost of resource,speed of construction and structural stability of the International Standard Organization(ISO)shipping containers in addressing the housing deficiency problem in the nation by meeting the basic need of shelter.Based on the hypothesis of being a cheaper alternative to the concrete and sand-crete blocks,which is the main construction technology used now,similar house types of both technologies were compared to ascertain the variation as part of the methodology for this research.The methodology also included literature reviews and case studies.The scope of this study was limited to 2-bedroom single-storey and multi-storey house types in Accra,the capital city of Ghana.In the final analysis however,this research proved that the container house is not cheaper than the traditional blockwork and concrete construction method is and is better used in temporary accommodation,in situations where time is essential.展开更多
In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share...In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share GPUs,which serve as coprocessors of central processing units(CPUs)and are activated only if tasks demand GPU computation.In a container environment,where resources can be shared among multiple users,GPU utilization can be increased by minimizing idle time because the tasks of many users run on a single GPU.However,unlike CPUs and memory,GPUs cannot logically multiplex their resources.Additionally,GPU memory does not support over-utilization:when it runs out,tasks will fail.Therefore,it is necessary to regulate the order of execution of concurrently running GPU tasks to avoid such task failures and to ensure equitable GPU sharing among users.In this paper,we propose a GPU task execution order management technique that controls GPU usage via time-based containers.The technique seeks to ensure equal GPU time among users in a container environment to prevent task failures.In the meantime,we use a deferred processing method to prevent GPU memory shortages when GPU tasks are executed simultaneously and to determine the execution order based on the GPU usage time.As the order of GPU tasks cannot be externally adjusted arbitrarily once the task commences,the GPU task is indirectly paused by pausing the container.In addition,as container pause/unpause status is based on the information about the available GPU memory capacity,overuse of GPU memory can be prevented at the source.As a result,the strategy can prevent task failure and the GPU tasks can be experimentally processed in appropriate order.展开更多
Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves a...Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves and fragments.To explore the protective effect of polyurea elastomer on LFC,the damage characteristics of polyurea coated liquid-filled container(PLFC)under the combined loading of blast shock wave and fragments were studied experimentally.The microstructure of the polyurea layer was observed by scanning electron microscopy,and the fracture and self-healing phenomena were analyzed.The simulation approach was used to explain the combined blast-and fragments-induced on the PLFC in detail.Finally,the effects of shock wave and fragment alone and in combination on the damage of PLFC were comprehensively compared.Results showed that the polyurea reduces the perforation rate of the fragment to the LFC,and the self-healing phenomenon could also reduce the liquid loss rate inside the container.The polyurea reduces the degree of depression in the center of the LFC,resulting in a decrease in the distance between adjacent fragments penetrating the LFC,and an increase in the probability of transfixion and fracture between holes.Under the close-in blast,the detonation shock wave reached the LFC before the fragment.Polyurea does not all have an enhanced effect on the protection of LFC.The presence of internal water enhances the anti-blast performance of the container,and the hydrodynamic ram(HRAM)formed by the fragment impacting the water aggravated the plastic deformation of the container.The combined action has an enhancement effect on the deformation of the LFC.The depth of the container depression was 27%higher than that of the blast shock wave alone;thus,it cannot be simply summarized as linear superposition.展开更多
Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositori...Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositories results in increased overload due to the adoption of cloud services.Moreover,the migration of applications on the cloud with optimized resource allocation is a herculean task even though it is employed for minimizing the dilemma of allocating resources.In this paper,a Fire Hawk Optimization enabled Deep Learning Scheme(FHOEDLS)is proposed for minimizing the overload and optimizing the resource allocation on the hybrid cloud container architecture for migrating interoperability based applications This FHOEDLS achieves the load prediction through the utilization of deep CNN-GRU-AM model for attaining resource allocation and better migration of applications.It specifically adopted the Fire Hawk Optimization Algorithm(FHOA)for optimizing the parameters that influence the factors that aid in better interoperable application migration with improved resource allocation and minimized overhead.It considered the factors of resource capacity,transmission cost,demand,and predicted load into account during the formulation of the objective function utilized for resource allocation and application migration.The cloud simulation of this FHOEDLS is achieved using a container,Virtual Machine(VM),and Physical Machine(PM).The results of this proposed FHOEDLS confirmed a better resource capability of 0.418 and a minimized load of 0.0061.展开更多
The impact of high-velocity penetrators into liquid-filled containers can generate hydrodynamic ram effects,potentially causing catastrophic structural damage to the container.Previous studies have primarily focused o...The impact of high-velocity penetrators into liquid-filled containers can generate hydrodynamic ram effects,potentially causing catastrophic structural damage to the container.Previous studies have primarily focused on undeformed penetrators,such as fragments or bullets,with limited attention directed toward shaped charge jets.This study investigates the penetration characteristics of shaped charge jets impacting behind-armor liquid-filled containers,with particular emphasis on jet-liquid interactions.A theoretical penetration model incorporating material compressibility and jet stretching was developed based on the virtual origin theory.A high-speed imaging experimental system was designed to capture the jet motion within the container.The impact process was numerically reproduced using ANSYS/LSDYNA,and the effects of standoff and overmatch on jet penetration were analyzed.The results reveal that jet stretching induced by increased standoff enhances the penetration velocity of the jet.A proportional relationship between the stretching factor(λ)and the overmatch parameter(I)was identified,withλranging from approximately 1.22 to 1.38 times I across the studied standoff range(80-220 mm).The findings offer a basis for future studies on the pressure distribution in the liquid and the structural response of containers.展开更多
Container-based virtualization technology has been more widely used in edge computing environments recently due to its advantages of lighter resource occupation, faster startup capability, and better resource utilizat...Container-based virtualization technology has been more widely used in edge computing environments recently due to its advantages of lighter resource occupation, faster startup capability, and better resource utilization efficiency. To meet the diverse needs of tasks, it usually needs to instantiate multiple network functions in the form of containers interconnect various generated containers to build a Container Cluster(CC). Then CCs will be deployed on edge service nodes with relatively limited resources. However, the increasingly complex and timevarying nature of tasks brings great challenges to optimal placement of CC. This paper regards the charges for various resources occupied by providing services as revenue, the service efficiency and energy consumption as cost, thus formulates a Mixed Integer Programming(MIP) model to describe the optimal placement of CC on edge service nodes. Furthermore, an Actor-Critic based Deep Reinforcement Learning(DRL) incorporating Graph Convolutional Networks(GCN) framework named as RL-GCN is proposed to solve the optimization problem. The framework obtains an optimal placement strategy through self-learning according to the requirements and objectives of the placement of CC. Particularly, through the introduction of GCN, the features of the association relationship between multiple containers in CCs can be effectively extracted to improve the quality of placement.The experiment results show that under different scales of service nodes and task requests, the proposed method can obtain the improved system performance in terms of placement error ratio, time efficiency of solution output and cumulative system revenue compared with other representative baseline methods.展开更多
Images and videos play an increasingly vital role in daily life and are widely utilized as key evidentiary sources in judicial investigations and forensic analysis.Simultaneously,advancements in image and video proces...Images and videos play an increasingly vital role in daily life and are widely utilized as key evidentiary sources in judicial investigations and forensic analysis.Simultaneously,advancements in image and video processing technologies have facilitated the widespread availability of powerful editing tools,such as Deepfakes,enabling anyone to easily create manipulated or fake visual content,which poses an enormous threat to social security and public trust.To verify the authenticity and integrity of images and videos,numerous approaches have been proposed,which are primarily based on content analysis and their effectiveness is susceptible to interference from various image or video post-processing operations.Recent research has highlighted the potential of file containers analysis as a promising forensic approach that offers efficient and interpretable results.However,there is still a lack of review articles on this kind of approach.In order to fill this gap,we present a comprehensive review of file containers-based image and video forensics in this paper.Specifically,we categorize the existing methods into two distinct stages,qualitative analysis and quantitative analysis.In addition,an overall framework is proposed to organize the exiting approaches.Then,the advantages and disadvantages of the schemes used across different forensic tasks are provided.Finally,we outline the trends in this research area,aiming to provide valuable insights and technical guidance for future research.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is at the forefront of the global spectrum of cancer incidence and mortality,with conventional therapies like tyrosine kinase inhibitors limited by resistance.Recent studies hav...BACKGROUND Hepatocellular carcinoma(HCC)is at the forefront of the global spectrum of cancer incidence and mortality,with conventional therapies like tyrosine kinase inhibitors limited by resistance.Recent studies have highlighted the promising anticancer effects of nitidine chloride(NC)against HCC.SAC3 domain containing 1(SAC3D1)is critical for centrosome replication and spindle formation.However,research on SAC3D1 in HCC and NC remains limited.AIM To investigate the mechanisms underlying SAC3D1’s role in HCC progression and evaluated its potential as a therapeutic target of NC.METHODS RNA sequencing(RNA-seq)identified SAC3D1 expression changes in HCC cells after NC treatment.Molecular docking was further employed to validate the direct binding between NC and SAC3D1.Additionally,HCC multicenter data(The Cancer Genome Atlas_GTEx,ArrayExpress),pathway analysis,Pearson correlation analysis and SAC3D1 in vitro knockdown experiments were integrated to explore the molecular mechanisms underlying SAC3D1's involvement in HCC progression.RESULTS RNA-seq showed that NC treatment significantly downregulated SAC3D1 expression[log2(fold change)=-0.95,P<0.05],with molecular docking revealing that NC directly bound to SAC3D1 proteins(binding energy:-9.7 kcal/mol).Enrichment analysis showed that most pathways were closely related to the cell cycle.Pearson correlation analysis indicated that SAC3D1 and cell cycle genes were significantly positively correlated(correlation coefficient≥0.3,P<0.05).SAC3D1 knockdown inhibited HCC cell invasion,migration,and proliferation by arresting cells in the S and G2/M phases.Flow cytometry confirmed that after SAC3D1 knockdown,the early and total apoptosis percentage of HCC cells decreased,while the late apoptosis percentage increased.CONCLUSION As a potential target of NC,SAC3D1 may inhibit HCC progression through cell cycle regulation following its downregulation by NC.展开更多
The passive containment heat removal system(PCS)is one of the key passive safety systems of China’s third-generation advanced pressurized water reactor-Hua-long Pressurized Reactor(HPR1000),used to prevent overpressu...The passive containment heat removal system(PCS)is one of the key passive safety systems of China’s third-generation advanced pressurized water reactor-Hua-long Pressurized Reactor(HPR1000),used to prevent overpressure of large concrete containment under severe accident scenarios.This paper provides an overview of the development of the HPR1000 passive containment heat removal system,including its operating principles and configuration,internal heat exchanger design,feasibility tests,engineering-scale PCS verification tests,comprehensive tests on PCS-containment coupling characteristics,among other key supporting studies.These extensive studies demonstrated that the PCS of HPR1000,which is designed based on flashing-driven open natural circulation and efficient condensation heat transfer theory,can work effectively and ensure the integrity of the containment under various accident scenarios.The system has been applied to Fuqing No.5 and No.6 nuclear power units and Zhangzhou No.1 and No.2 units of China’s first million-kilowatt third-generation nuclear power HPR1000.It is also applied to K-2/K-3 units of Karachi Nuclear Power Plant in Pakistan.展开更多
The evolution mechanism of railway transportation network nodes driven by sea-rail intermodal transport(SRIT),a globally prevalent logistics method,has not been thoroughly investigated.From the perspective of SRIT,thi...The evolution mechanism of railway transportation network nodes driven by sea-rail intermodal transport(SRIT),a globally prevalent logistics method,has not been thoroughly investigated.From the perspective of SRIT,this study constructed a framework for understanding the evolution of railway container transport network nodes using Northeast China from 2013 to 2020 as a case study.It leverages proprietary data from 95306 Railway Freight E-commerce Platform.By employing the hybrid EWM-GA-TOPSIS model,complex network analysis,modified gravity model,and correlation and regression analyses,this study delves into the spatiotemporal patterns and dynamic transformations of railway container freight stations(RCFS).Finally,the long-term relationship between the RCFS and SRIT is explored.The results indicate that the spatial and temporal analysis of the RCFS in Northeast China from 2013 to 2020 revealed a clear polarisation trend,with the top-ranked stations mainly concentrated near ports and important transportation hubs.Additionally,the RCFS exhibited an expansionary trend;however,its development was uneven,and there was a significant increase in the number of new stations compared to abandoned stations,indicating an overall positive growth tendency.Moreover,the intensity of the SRIT at the RCFS in Northeast China notably increased.A significant positive linear relationship exists between SRIT and the freight capacity of all stations.A relatively pronounced correlation was observed for high-intensity stations,whereas a relatively weak correlation was observed for low-intensity stations.This study not only provides an effective framework for future research on RCFS within the context of SRIT but also serves as a scientific reference for promoting the implementation of the national strategy for multimodal transportation.展开更多
Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transport...Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transportation capabilities,allowing them tomove in the opposite directionwithout turning around,which helps reduce transportation time.This paper aims at the problem of AGV scheduling and bidirectional conflict-free routing with battery swapping in automated terminals.A bi-level mixed integer programming(MIP)model is proposed,taking into account task assignment,bidirectional conflict-free routing,and battery swapping.The upper model focuses on container task assignment and AGV battery swapping planning,while the lower model ensures conflict-free movement of AGVs.A double-threshold battery swapping strategy is introduced,allowing AGVs to utilize waiting time for loading for battery swapping.An improved differential evolution variable neighborhood search(IDE-VNS)algorithm is developed to solve the bi-level MIP model,aiming to minimize the completion time of all jobs.Experimental results demonstrate that compared to the differential evolution(DE)algorithm and the genetic algorithm(GA),the IDEVNS algorithmreduces fitness values by 44.49% and 45.22%,though it does increase computation time by 56.28% and 62.03%,respectively.Bidirectional transportation reduces the fitness value by an average of 10.97% when the container scale is small.As the container scale increases,the fitness value of bidirectional transportation gradually approaches that of unidirectional transportation.The results further show that the double-threshold battery swapping strategy enhances AGV utilization and reduces the fitness value.展开更多
Structural properties of the ship container logistics network of China(SCLNC)are studied in the light of recent investigations of complex networks.SCLNC is composed of a set of routes and ports located along the sea o...Structural properties of the ship container logistics network of China(SCLNC)are studied in the light of recent investigations of complex networks.SCLNC is composed of a set of routes and ports located along the sea or river.Network properties including the degree distribution,degree correlations,clustering,shortest path length,centrality and betweenness are studied in different definition of network topology.It is found that geographical constraint plays an important role in the network topology of SCLNC.We also study the traffic flow of SCLNC based on the weighted network representation,and demonstrate the weight distribution can be described by power law or exponential function depending on the assumed definition of network topology.Other features related to SCLNC are also investigated.展开更多
BACKGROUND Metabolic dysregulation is considered a significant hallmark of hepatocellular carcinoma(HCC).SAC3 domain containing 1(SAC3D1)functions in the cell cycle,and its expression is upregulated in various cancers...BACKGROUND Metabolic dysregulation is considered a significant hallmark of hepatocellular carcinoma(HCC).SAC3 domain containing 1(SAC3D1)functions in the cell cycle,and its expression is upregulated in various cancers.It is known that metabolic changes occur at different stages of the cell cycle to maintain the biosynthesis and replication of both normal and cancer cells.Based on the role of SAC3D1 in mitosis,we hypothesize that abnormal expression of SAC3D1 may affect cellular metabolism.However,it remains unclear whether SAC3D1 mediates the progression of HCC by regulating metabolic reprogramming.AIM To comprehensively elucidate the impact and molecular mechanism of SAC3D1 on the progression of HCC by regulating the metabolic reprogramming.METHODS The constructed SAC3D1 overexpression and knockdown HCC cell lines were used for detecting cell proliferation,migration capabilities,as well as glycolysis and adenosine triphosphate(ATP)production rate assays.They were also employed for examining molecular markers associated with cell migration and glycolysis.The transcriptome sequencing data of cells have revealed the pathways potentially influenced by SAC3D1.The tail vein metastasis model and xenograft tumor experiments were utilized to demonstrate SAC3D1’s tumor-promoting effects in vivo.RESULTS SAC3D1 expression was upregulated and associated with poor prognosis in HCC patients.SAC3D1 enhanced the proliferation and migration abilities and reduced the population dependence of HCC cells in vitro and in vivo.The upregulation of SAC3D1 enhanced cellular glycolysis and ATP production.The cell transcriptome sequencing data revealed that SAC3D1 activated Wnt signaling pathway.SAC3D1 did not modulate the transcription ofβ-Catenin,while might inhibit its degradation.Further investigations indicated that the increase of SAC3D1 leads to moreβ-Catenin accumulating in the nucleus,facilitating the expression of c-Myc,one of the upstream regulatory factors of glycolysis.The iCRT3,an antagonist ofβ-Catenin,could counteract the increase of c-Myc induced by SAC3D1,while also downregulating the expression of glycolysis-related proteins.CONCLUSION This study found that SAC3D1 enhances HCC cell glycolysis and ATP production via theβ-Catenin/c-Myc signaling axis,thereby promoting the progression of HCC.展开更多
BACKGROUND The mortality rate for severe cases of acute pancreatitis(AP),a common gastrointestinal emergency,is as high as 30%.Our previous study has shown that rutaecarpine(Rut)has a therapeutic effect on AP.AIM To i...BACKGROUND The mortality rate for severe cases of acute pancreatitis(AP),a common gastrointestinal emergency,is as high as 30%.Our previous study has shown that rutaecarpine(Rut)has a therapeutic effect on AP.AIM To investigate the role of F-box and WD repeat domain containing 11(FBXW11)in AP models and to assess whether Rut mitigates AP by regulating FBXW11.METHODS AP rat model was established and treated with Rut,followed by biochemical analysis of serum amylase and lipase,hematoxylin and eosin staining of pancreatic tissue,and immunohistochemistry detection of pancreatic Ly6G,CD11b,and myeloperoxidase.Assay kits were used to detect oxidative stress-related indicators in pancreatic tissue and inflammatory factors in serum.AR42J cells were treated with cerulein to model AP and subjected to Cell Counting Kit-8 viability assay,flow cytometry apoptosis assay,and immunofluorescence detection of reactive oxygen species to elucidate the mechanistic involvement of the enhancer of zeste homolog 2(EZH2)-FBXW11 axis in Rut-mediated protection against AP.The EZH2-histone H3 binding and H3 methylation were evaluated using co-immunoprecipitation.RESULTS Rut treatment ameliorated AP severity,as evidenced by reduced serum levels of pancreatic enzymes(amylase and lipase)and attenuated histological damage.Rut also decreased inflammatory markers(interleukin-1 beta,interleukin-6,and tumor necrosis factor alpha),tissue oxidative stress(malondialdehyde),and neutrophil infiltration(Ly6G,CD11b,and myeloperoxidase)levels in rats with AP.Moreover,Rut restored pancreatic antioxidant capacity(glutathione and superoxide dismutase).In vitro,Rut pre-incubation enhanced cell viability and suppressed cerulein-induced apoptosis and oxidative stress.Rut increased EZH2 expression while decreasing FBXW11 expression.FBXW11 overexpression eliminated the protective effect of Rut against AP.Further analysis revealed that EZH2 binds to H3 and upregulates H3 methylation levels,thereby inhibiting FBXW11 expression.CONCLUSION Collectively,our findings demonstrate that Rut ameliorates AP by upregulating EZH2,thereby enhancing H3 methylation and suppressing FBXW11 expression.展开更多
An Interval Type-2(IT-2)fuzzy controller design approach is proposed in this research to simultaneously achievemultiple control objectives inNonlinearMulti-Agent Systems(NMASs),including formation,containment,and coll...An Interval Type-2(IT-2)fuzzy controller design approach is proposed in this research to simultaneously achievemultiple control objectives inNonlinearMulti-Agent Systems(NMASs),including formation,containment,and collision avoidance.However,inherent nonlinearities and uncertainties present in practical control systems contribute to the challenge of achieving precise control performance.Based on the IT-2 Takagi-Sugeno Fuzzy Model(T-SFM),the fuzzy control approach can offer a more effective solution for NMASs facing uncertainties.Unlike existing control methods for NMASs,the Formation and Containment(F-and-C)control problem with collision avoidance capability under uncertainties based on the IT-2 T-SFM is discussed for the first time.Moreover,an IT-2 fuzzy tracking control approach is proposed to solve the formation task for leaders in NMASs without requiring communication.This control scheme makes the design process of the IT-2 fuzzy Formation Controller(FC)more straightforward and effective.According to the communication interaction protocol,the IT-2 Containment Controller(CC)design approach is proposed for followers to ensure convergence into the region defined by the leaders.Leveraging the IT-2 T-SFM representation,the analysis methods developed for linear Multi-Agent Systems(MASs)are successfully extended to perform containment analysis without requiring the additional assumptions imposed in existing research.Notably,the IT-2 fuzzy tracking controller can also be applied in collision avoidance situations to track the desired trajectories calculated by the avoidance algorithm under the Artificial Potential Field(APF).Benefiting from the combination of vortex and source APFs,the leaders can properly adjust the system dynamics to prevent potential collision risk.Integrating the fuzzy theory and APFs avoidance algorithm,an IT-2 fuzzy controller design approach is proposed to achieve the F-and-C purposewhile ensuring collision avoidance capability.Finally,amulti-ship simulation is conducted to validate the feasibility and effectiveness of the designed IT-2 fuzzy controller.展开更多
BACKGROUND The role of NLR family pyrin domain containing 3(NLRP3)in post-endoscopic submucosal dissection(ESD)esophageal stricture remains incompletely understood.The effect of celastrol(CEL)on the prevention of esop...BACKGROUND The role of NLR family pyrin domain containing 3(NLRP3)in post-endoscopic submucosal dissection(ESD)esophageal stricture remains incompletely understood.The effect of celastrol(CEL)on the prevention of esophageal strictures has not yet been investigated.AIM To explore the effect of CEL on the prevention of esophageal stricture in rats.METHODS NLRP3,interleukin(IL)-1β,and IL-18 mRNA levels were measured in patients’tissues after esophageal ESD.NLRP3 expression in esophageal fibroblasts was determined using immunohistochemistry and immunofluorescence staining.Lentiviral transfection was used to induce NLRP3 overexpression and thioredoxin reductase 1(TXNRD1)silencing.The CCK8 assay was used to determine the optimal CEL concentration.Reactive oxygen species(ROS)generation was detected via fluorescence and flow cytometry.Masson’s trichrome staining and barium esophagography were performed to assess collagen deposition and esophageal stenosis.RESULTS The mRNA levels of NLRP3 and IL-1βwere higher in human tissues from the ESD resection bed than in normal esophageal mucosa.NLRP3 overexpression in primary rat esophageal fibroblasts led to high collagen 1 expression.Thus,NLRP3 participated in esophageal inflammation and tissue repair after ESD.Comparable to prednisolone,CEL significantly inhibited NLRP3 activation in vitro and in vivo,and esophageal strictures were markedly alleviated.Mechanistically,CEL upregulated TXNRD1 expression and reduced ROS production,thereby inhibiting NLRP3 expression.This effect was reversed by TXNRD1 silencing.Furthermore,TXNRD1 interacted with NLRP3 and promoted its ubiquitination.CONCLUSION CEL is a promising alternative therapeutic agent for the prevention of post-ESD esophageal strictures.展开更多
BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untran...BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.展开更多
An innovative multi-layer composite explosion containment vessel(CECV)utilizing a sliding steel platealuminum honeycomb-fiber cloth sandwich is put forward to improve the anti-explosion capacity of a conventional sing...An innovative multi-layer composite explosion containment vessel(CECV)utilizing a sliding steel platealuminum honeycomb-fiber cloth sandwich is put forward to improve the anti-explosion capacity of a conventional single-layer explosion containment vessel(SECV).Firstly,a series of experiments and finite element(FE)simulations of internal explosions are implemented to understand the basic anti-explosion characteristics of a SECV and the rationality of the computational models and methods is verified by the comparison between the experimental results and simulation results.Based on this,the CECV is designed in detail and a variety of FE simulations are carried out to investigate effects of the sandwich structure,the explosive quantity and the laying mode of the fiber cloth on anti-explosion performance and dynamic response of the CECV under internal explosions.Simulation results indicate that the end cover is the critical position for both the SECV and CECV.The maximum pressure of the explosion shock wave and the maximum strain of the CECV can be extremely declined compared to those of the SECV.As a result,the explosive quantity the CECV can sustain is up to 20 times of that the SECV can sustain.Besides,as the explosive quantity increases,the internal pressure of the CECV keeps growing and the plastic deformation and failure of the sandwich structure become more and more severe,yielding plastic strain of the CECV in addition to elastic strain.The results also reveal that the laying angles of the fiber cloth's five layers have an impact on the anti-explosion performance of the CECV.For example,the CECV with fiber cloth layered in 0°/45°/90°/45°/0°mode has the optimal anti-capacity,compared to 0°/0°/0°/0°/0°and 0°/30°/60°/30°/0°modes.Overall,owing to remarkable anti-explosion capacity,this CECV can be regarded as a promising candidate for explosion resistance.展开更多
基金financial support from the National Natural Science Foundation of China(Grant No.11572159).
文摘The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure variations,potentially causing catastrophic damage to the container.Current studies mainly focus on non-deforming projectiles,such as fragments,with limited exploration of shaped charge jets.In this paper,a uniquely experimental system was designed to record cavity profiles in behind-armor liquid-filled containers subjected to shaped charge jet impacts.The impact process was then numerically reproduced using the explicit simulation program ANSYS LS-DYNA with the Structured Arbitrary Lagrangian-Eulerian(S-ALE)solver.The formation mechanism,along with the dimensional and shape evolution of the cavity was investigated.Additionally,the influence of the impact kinetic energy of the jet on the cavity characteristics was analyzed.The findings reveal that the cavity profile exhibits a conical shape,primarily driven by direct jet impact and inertial effects.The expansion rates of both cavity length and maximum radius increase with jet impact kinetic energy.When the impact kinetic energy is reduced to 28.2 kJ or below,the length-to-diameter ratio of the cavity ultimately stabilizes at approximately 7.
基金supported by the National Natural Science Foundation of China,No.82104412(to TD)Shaanxi Provincial Key R&D Program,No.2023-YBSF-165(to TD)+1 种基金the Natural Science Foundation of Shaanxi Department of Science and Technology,No.2018JM7022(to FM)Shaanxi Provincial Key Industry Chain Project,No.2021ZDLSF04-11(to PW)。
文摘The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.
文摘The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The idea of using shipping containers as a building component is by no means new in the Accra Metropolis as most shipping containers are re-constructed architecturally and used for temporary accommodation needs like storage,make-shift shops,emergency shelters and site offices.The concept of using these shipping containers as modular building components in architecture however,is still foreign to building practitioners and the nation at large.This research paper set out to use the containers not for luxury apartments but to harness the merits of availability,low-cost of resource,speed of construction and structural stability of the International Standard Organization(ISO)shipping containers in addressing the housing deficiency problem in the nation by meeting the basic need of shelter.Based on the hypothesis of being a cheaper alternative to the concrete and sand-crete blocks,which is the main construction technology used now,similar house types of both technologies were compared to ascertain the variation as part of the methodology for this research.The methodology also included literature reviews and case studies.The scope of this study was limited to 2-bedroom single-storey and multi-storey house types in Accra,the capital city of Ghana.In the final analysis however,this research proved that the container house is not cheaper than the traditional blockwork and concrete construction method is and is better used in temporary accommodation,in situations where time is essential.
基金supported by“Regional Innovation Strategy(RIS)”through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(MOE)(2023RIS-009).
文摘In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share GPUs,which serve as coprocessors of central processing units(CPUs)and are activated only if tasks demand GPU computation.In a container environment,where resources can be shared among multiple users,GPU utilization can be increased by minimizing idle time because the tasks of many users run on a single GPU.However,unlike CPUs and memory,GPUs cannot logically multiplex their resources.Additionally,GPU memory does not support over-utilization:when it runs out,tasks will fail.Therefore,it is necessary to regulate the order of execution of concurrently running GPU tasks to avoid such task failures and to ensure equitable GPU sharing among users.In this paper,we propose a GPU task execution order management technique that controls GPU usage via time-based containers.The technique seeks to ensure equal GPU time among users in a container environment to prevent task failures.In the meantime,we use a deferred processing method to prevent GPU memory shortages when GPU tasks are executed simultaneously and to determine the execution order based on the GPU usage time.As the order of GPU tasks cannot be externally adjusted arbitrarily once the task commences,the GPU task is indirectly paused by pausing the container.In addition,as container pause/unpause status is based on the information about the available GPU memory capacity,overuse of GPU memory can be prevented at the source.As a result,the strategy can prevent task failure and the GPU tasks can be experimentally processed in appropriate order.
基金supported by the National Natural Science Foundation of China(Grant Nos.12102480,52278543 and 51978660)Natural Science Foundation of Jiangsu Province(Grant No.BK20231489)。
文摘Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves and fragments.To explore the protective effect of polyurea elastomer on LFC,the damage characteristics of polyurea coated liquid-filled container(PLFC)under the combined loading of blast shock wave and fragments were studied experimentally.The microstructure of the polyurea layer was observed by scanning electron microscopy,and the fracture and self-healing phenomena were analyzed.The simulation approach was used to explain the combined blast-and fragments-induced on the PLFC in detail.Finally,the effects of shock wave and fragment alone and in combination on the damage of PLFC were comprehensively compared.Results showed that the polyurea reduces the perforation rate of the fragment to the LFC,and the self-healing phenomenon could also reduce the liquid loss rate inside the container.The polyurea reduces the degree of depression in the center of the LFC,resulting in a decrease in the distance between adjacent fragments penetrating the LFC,and an increase in the probability of transfixion and fracture between holes.Under the close-in blast,the detonation shock wave reached the LFC before the fragment.Polyurea does not all have an enhanced effect on the protection of LFC.The presence of internal water enhances the anti-blast performance of the container,and the hydrodynamic ram(HRAM)formed by the fragment impacting the water aggravated the plastic deformation of the container.The combined action has an enhancement effect on the deformation of the LFC.The depth of the container depression was 27%higher than that of the blast shock wave alone;thus,it cannot be simply summarized as linear superposition.
文摘Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositories results in increased overload due to the adoption of cloud services.Moreover,the migration of applications on the cloud with optimized resource allocation is a herculean task even though it is employed for minimizing the dilemma of allocating resources.In this paper,a Fire Hawk Optimization enabled Deep Learning Scheme(FHOEDLS)is proposed for minimizing the overload and optimizing the resource allocation on the hybrid cloud container architecture for migrating interoperability based applications This FHOEDLS achieves the load prediction through the utilization of deep CNN-GRU-AM model for attaining resource allocation and better migration of applications.It specifically adopted the Fire Hawk Optimization Algorithm(FHOA)for optimizing the parameters that influence the factors that aid in better interoperable application migration with improved resource allocation and minimized overhead.It considered the factors of resource capacity,transmission cost,demand,and predicted load into account during the formulation of the objective function utilized for resource allocation and application migration.The cloud simulation of this FHOEDLS is achieved using a container,Virtual Machine(VM),and Physical Machine(PM).The results of this proposed FHOEDLS confirmed a better resource capability of 0.418 and a minimized load of 0.0061.
基金the financial support from the National Natural Science Foundation of China(Grant No.11572159)。
文摘The impact of high-velocity penetrators into liquid-filled containers can generate hydrodynamic ram effects,potentially causing catastrophic structural damage to the container.Previous studies have primarily focused on undeformed penetrators,such as fragments or bullets,with limited attention directed toward shaped charge jets.This study investigates the penetration characteristics of shaped charge jets impacting behind-armor liquid-filled containers,with particular emphasis on jet-liquid interactions.A theoretical penetration model incorporating material compressibility and jet stretching was developed based on the virtual origin theory.A high-speed imaging experimental system was designed to capture the jet motion within the container.The impact process was numerically reproduced using ANSYS/LSDYNA,and the effects of standoff and overmatch on jet penetration were analyzed.The results reveal that jet stretching induced by increased standoff enhances the penetration velocity of the jet.A proportional relationship between the stretching factor(λ)and the overmatch parameter(I)was identified,withλranging from approximately 1.22 to 1.38 times I across the studied standoff range(80-220 mm).The findings offer a basis for future studies on the pressure distribution in the liquid and the structural response of containers.
文摘Container-based virtualization technology has been more widely used in edge computing environments recently due to its advantages of lighter resource occupation, faster startup capability, and better resource utilization efficiency. To meet the diverse needs of tasks, it usually needs to instantiate multiple network functions in the form of containers interconnect various generated containers to build a Container Cluster(CC). Then CCs will be deployed on edge service nodes with relatively limited resources. However, the increasingly complex and timevarying nature of tasks brings great challenges to optimal placement of CC. This paper regards the charges for various resources occupied by providing services as revenue, the service efficiency and energy consumption as cost, thus formulates a Mixed Integer Programming(MIP) model to describe the optimal placement of CC on edge service nodes. Furthermore, an Actor-Critic based Deep Reinforcement Learning(DRL) incorporating Graph Convolutional Networks(GCN) framework named as RL-GCN is proposed to solve the optimization problem. The framework obtains an optimal placement strategy through self-learning according to the requirements and objectives of the placement of CC. Particularly, through the introduction of GCN, the features of the association relationship between multiple containers in CCs can be effectively extracted to improve the quality of placement.The experiment results show that under different scales of service nodes and task requests, the proposed method can obtain the improved system performance in terms of placement error ratio, time efficiency of solution output and cumulative system revenue compared with other representative baseline methods.
基金supported in part by Natural Science Foundation of Hubei Province of China under Grant 2023AFB016the 2022 Opening Fund for Hubei Key Laboratory of Intelligent Vision Based Monitoring for Hydroelectric Engineering under Grant 2022SDSJ02the Construction Fund for Hubei Key Laboratory of Intelligent Vision Based Monitoring for Hydroelectric Engineering under Grant 2019ZYYD007.
文摘Images and videos play an increasingly vital role in daily life and are widely utilized as key evidentiary sources in judicial investigations and forensic analysis.Simultaneously,advancements in image and video processing technologies have facilitated the widespread availability of powerful editing tools,such as Deepfakes,enabling anyone to easily create manipulated or fake visual content,which poses an enormous threat to social security and public trust.To verify the authenticity and integrity of images and videos,numerous approaches have been proposed,which are primarily based on content analysis and their effectiveness is susceptible to interference from various image or video post-processing operations.Recent research has highlighted the potential of file containers analysis as a promising forensic approach that offers efficient and interpretable results.However,there is still a lack of review articles on this kind of approach.In order to fill this gap,we present a comprehensive review of file containers-based image and video forensics in this paper.Specifically,we categorize the existing methods into two distinct stages,qualitative analysis and quantitative analysis.In addition,an overall framework is proposed to organize the exiting approaches.Then,the advantages and disadvantages of the schemes used across different forensic tasks are provided.Finally,we outline the trends in this research area,aiming to provide valuable insights and technical guidance for future research.
基金Supported by National Natural Science Foundation of China,No.82160762 and No.82460783Guangxi Medical University“Four New”Project,No.SX202403+2 种基金Innovation Project of Guangxi Graduate Education,No.JGY2023068Guangxi Higher Education Undergraduate Teaching Reform Project,No.2022JGA146China Undergraduate Innovation and Entrepreneurship Training Program,No.202310598045.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is at the forefront of the global spectrum of cancer incidence and mortality,with conventional therapies like tyrosine kinase inhibitors limited by resistance.Recent studies have highlighted the promising anticancer effects of nitidine chloride(NC)against HCC.SAC3 domain containing 1(SAC3D1)is critical for centrosome replication and spindle formation.However,research on SAC3D1 in HCC and NC remains limited.AIM To investigate the mechanisms underlying SAC3D1’s role in HCC progression and evaluated its potential as a therapeutic target of NC.METHODS RNA sequencing(RNA-seq)identified SAC3D1 expression changes in HCC cells after NC treatment.Molecular docking was further employed to validate the direct binding between NC and SAC3D1.Additionally,HCC multicenter data(The Cancer Genome Atlas_GTEx,ArrayExpress),pathway analysis,Pearson correlation analysis and SAC3D1 in vitro knockdown experiments were integrated to explore the molecular mechanisms underlying SAC3D1's involvement in HCC progression.RESULTS RNA-seq showed that NC treatment significantly downregulated SAC3D1 expression[log2(fold change)=-0.95,P<0.05],with molecular docking revealing that NC directly bound to SAC3D1 proteins(binding energy:-9.7 kcal/mol).Enrichment analysis showed that most pathways were closely related to the cell cycle.Pearson correlation analysis indicated that SAC3D1 and cell cycle genes were significantly positively correlated(correlation coefficient≥0.3,P<0.05).SAC3D1 knockdown inhibited HCC cell invasion,migration,and proliferation by arresting cells in the S and G2/M phases.Flow cytometry confirmed that after SAC3D1 knockdown,the early and total apoptosis percentage of HCC cells decreased,while the late apoptosis percentage increased.CONCLUSION As a potential target of NC,SAC3D1 may inhibit HCC progression through cell cycle regulation following its downregulation by NC.
基金supported by China Nuclear Power Engineering Co.,Ltd.and Harbin Engineering University,and granted financial resources by China’s National Energy Administration and China National Nuclear Corporation.
文摘The passive containment heat removal system(PCS)is one of the key passive safety systems of China’s third-generation advanced pressurized water reactor-Hua-long Pressurized Reactor(HPR1000),used to prevent overpressure of large concrete containment under severe accident scenarios.This paper provides an overview of the development of the HPR1000 passive containment heat removal system,including its operating principles and configuration,internal heat exchanger design,feasibility tests,engineering-scale PCS verification tests,comprehensive tests on PCS-containment coupling characteristics,among other key supporting studies.These extensive studies demonstrated that the PCS of HPR1000,which is designed based on flashing-driven open natural circulation and efficient condensation heat transfer theory,can work effectively and ensure the integrity of the containment under various accident scenarios.The system has been applied to Fuqing No.5 and No.6 nuclear power units and Zhangzhou No.1 and No.2 units of China’s first million-kilowatt third-generation nuclear power HPR1000.It is also applied to K-2/K-3 units of Karachi Nuclear Power Plant in Pakistan.
基金National Natural Science Foundation of ChinaNo.72174035+5 种基金The National Key Research and Development ProjectNo.2023YFB4302200111 Project of ChinaNo.B20082The Talent Planning in DalianNo.2022RG05。
文摘The evolution mechanism of railway transportation network nodes driven by sea-rail intermodal transport(SRIT),a globally prevalent logistics method,has not been thoroughly investigated.From the perspective of SRIT,this study constructed a framework for understanding the evolution of railway container transport network nodes using Northeast China from 2013 to 2020 as a case study.It leverages proprietary data from 95306 Railway Freight E-commerce Platform.By employing the hybrid EWM-GA-TOPSIS model,complex network analysis,modified gravity model,and correlation and regression analyses,this study delves into the spatiotemporal patterns and dynamic transformations of railway container freight stations(RCFS).Finally,the long-term relationship between the RCFS and SRIT is explored.The results indicate that the spatial and temporal analysis of the RCFS in Northeast China from 2013 to 2020 revealed a clear polarisation trend,with the top-ranked stations mainly concentrated near ports and important transportation hubs.Additionally,the RCFS exhibited an expansionary trend;however,its development was uneven,and there was a significant increase in the number of new stations compared to abandoned stations,indicating an overall positive growth tendency.Moreover,the intensity of the SRIT at the RCFS in Northeast China notably increased.A significant positive linear relationship exists between SRIT and the freight capacity of all stations.A relatively pronounced correlation was observed for high-intensity stations,whereas a relatively weak correlation was observed for low-intensity stations.This study not only provides an effective framework for future research on RCFS within the context of SRIT but also serves as a scientific reference for promoting the implementation of the national strategy for multimodal transportation.
基金supported by National Natural Science Foundation of China(No.62073212)Shanghai Science and Technology Commission(No.23ZR1426600).
文摘Automated guided vehicles(AGVs)are key equipment in automated container terminals(ACTs),and their operational efficiency can be impacted by conflicts and battery swapping.Additionally,AGVs have bidirectional transportation capabilities,allowing them tomove in the opposite directionwithout turning around,which helps reduce transportation time.This paper aims at the problem of AGV scheduling and bidirectional conflict-free routing with battery swapping in automated terminals.A bi-level mixed integer programming(MIP)model is proposed,taking into account task assignment,bidirectional conflict-free routing,and battery swapping.The upper model focuses on container task assignment and AGV battery swapping planning,while the lower model ensures conflict-free movement of AGVs.A double-threshold battery swapping strategy is introduced,allowing AGVs to utilize waiting time for loading for battery swapping.An improved differential evolution variable neighborhood search(IDE-VNS)algorithm is developed to solve the bi-level MIP model,aiming to minimize the completion time of all jobs.Experimental results demonstrate that compared to the differential evolution(DE)algorithm and the genetic algorithm(GA),the IDEVNS algorithmreduces fitness values by 44.49% and 45.22%,though it does increase computation time by 56.28% and 62.03%,respectively.Bidirectional transportation reduces the fitness value by an average of 10.97% when the container scale is small.As the container scale increases,the fitness value of bidirectional transportation gradually approaches that of unidirectional transportation.The results further show that the double-threshold battery swapping strategy enhances AGV utilization and reduces the fitness value.
基金supported by Youth Foundation for Research of the Waterborne Transportation Institute.
文摘Structural properties of the ship container logistics network of China(SCLNC)are studied in the light of recent investigations of complex networks.SCLNC is composed of a set of routes and ports located along the sea or river.Network properties including the degree distribution,degree correlations,clustering,shortest path length,centrality and betweenness are studied in different definition of network topology.It is found that geographical constraint plays an important role in the network topology of SCLNC.We also study the traffic flow of SCLNC based on the weighted network representation,and demonstrate the weight distribution can be described by power law or exponential function depending on the assumed definition of network topology.Other features related to SCLNC are also investigated.
基金Supported by the Shanghai Yangpu District Science and Technology Commission,No.YPQ202303Shanghai Municipal Health Commission Clinical Research Special Project,No.202240122Shanghai Medical Innovation Research Special Project,No.22Y11908600.
文摘BACKGROUND Metabolic dysregulation is considered a significant hallmark of hepatocellular carcinoma(HCC).SAC3 domain containing 1(SAC3D1)functions in the cell cycle,and its expression is upregulated in various cancers.It is known that metabolic changes occur at different stages of the cell cycle to maintain the biosynthesis and replication of both normal and cancer cells.Based on the role of SAC3D1 in mitosis,we hypothesize that abnormal expression of SAC3D1 may affect cellular metabolism.However,it remains unclear whether SAC3D1 mediates the progression of HCC by regulating metabolic reprogramming.AIM To comprehensively elucidate the impact and molecular mechanism of SAC3D1 on the progression of HCC by regulating the metabolic reprogramming.METHODS The constructed SAC3D1 overexpression and knockdown HCC cell lines were used for detecting cell proliferation,migration capabilities,as well as glycolysis and adenosine triphosphate(ATP)production rate assays.They were also employed for examining molecular markers associated with cell migration and glycolysis.The transcriptome sequencing data of cells have revealed the pathways potentially influenced by SAC3D1.The tail vein metastasis model and xenograft tumor experiments were utilized to demonstrate SAC3D1’s tumor-promoting effects in vivo.RESULTS SAC3D1 expression was upregulated and associated with poor prognosis in HCC patients.SAC3D1 enhanced the proliferation and migration abilities and reduced the population dependence of HCC cells in vitro and in vivo.The upregulation of SAC3D1 enhanced cellular glycolysis and ATP production.The cell transcriptome sequencing data revealed that SAC3D1 activated Wnt signaling pathway.SAC3D1 did not modulate the transcription ofβ-Catenin,while might inhibit its degradation.Further investigations indicated that the increase of SAC3D1 leads to moreβ-Catenin accumulating in the nucleus,facilitating the expression of c-Myc,one of the upstream regulatory factors of glycolysis.The iCRT3,an antagonist ofβ-Catenin,could counteract the increase of c-Myc induced by SAC3D1,while also downregulating the expression of glycolysis-related proteins.CONCLUSION This study found that SAC3D1 enhances HCC cell glycolysis and ATP production via theβ-Catenin/c-Myc signaling axis,thereby promoting the progression of HCC.
基金Supported by Hunan Provincial Natural Science Foundation of China,No.2022JJ40836the Key Project of Research and Development Plan of Hunan Province,No.2023DK2002the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation,No.GZC20242045.
文摘BACKGROUND The mortality rate for severe cases of acute pancreatitis(AP),a common gastrointestinal emergency,is as high as 30%.Our previous study has shown that rutaecarpine(Rut)has a therapeutic effect on AP.AIM To investigate the role of F-box and WD repeat domain containing 11(FBXW11)in AP models and to assess whether Rut mitigates AP by regulating FBXW11.METHODS AP rat model was established and treated with Rut,followed by biochemical analysis of serum amylase and lipase,hematoxylin and eosin staining of pancreatic tissue,and immunohistochemistry detection of pancreatic Ly6G,CD11b,and myeloperoxidase.Assay kits were used to detect oxidative stress-related indicators in pancreatic tissue and inflammatory factors in serum.AR42J cells were treated with cerulein to model AP and subjected to Cell Counting Kit-8 viability assay,flow cytometry apoptosis assay,and immunofluorescence detection of reactive oxygen species to elucidate the mechanistic involvement of the enhancer of zeste homolog 2(EZH2)-FBXW11 axis in Rut-mediated protection against AP.The EZH2-histone H3 binding and H3 methylation were evaluated using co-immunoprecipitation.RESULTS Rut treatment ameliorated AP severity,as evidenced by reduced serum levels of pancreatic enzymes(amylase and lipase)and attenuated histological damage.Rut also decreased inflammatory markers(interleukin-1 beta,interleukin-6,and tumor necrosis factor alpha),tissue oxidative stress(malondialdehyde),and neutrophil infiltration(Ly6G,CD11b,and myeloperoxidase)levels in rats with AP.Moreover,Rut restored pancreatic antioxidant capacity(glutathione and superoxide dismutase).In vitro,Rut pre-incubation enhanced cell viability and suppressed cerulein-induced apoptosis and oxidative stress.Rut increased EZH2 expression while decreasing FBXW11 expression.FBXW11 overexpression eliminated the protective effect of Rut against AP.Further analysis revealed that EZH2 binds to H3 and upregulates H3 methylation levels,thereby inhibiting FBXW11 expression.CONCLUSION Collectively,our findings demonstrate that Rut ameliorates AP by upregulating EZH2,thereby enhancing H3 methylation and suppressing FBXW11 expression.
基金founded by the National Science and Technology Council of the Republic of China under contract NSTC113-2221-E-019-032.
文摘An Interval Type-2(IT-2)fuzzy controller design approach is proposed in this research to simultaneously achievemultiple control objectives inNonlinearMulti-Agent Systems(NMASs),including formation,containment,and collision avoidance.However,inherent nonlinearities and uncertainties present in practical control systems contribute to the challenge of achieving precise control performance.Based on the IT-2 Takagi-Sugeno Fuzzy Model(T-SFM),the fuzzy control approach can offer a more effective solution for NMASs facing uncertainties.Unlike existing control methods for NMASs,the Formation and Containment(F-and-C)control problem with collision avoidance capability under uncertainties based on the IT-2 T-SFM is discussed for the first time.Moreover,an IT-2 fuzzy tracking control approach is proposed to solve the formation task for leaders in NMASs without requiring communication.This control scheme makes the design process of the IT-2 fuzzy Formation Controller(FC)more straightforward and effective.According to the communication interaction protocol,the IT-2 Containment Controller(CC)design approach is proposed for followers to ensure convergence into the region defined by the leaders.Leveraging the IT-2 T-SFM representation,the analysis methods developed for linear Multi-Agent Systems(MASs)are successfully extended to perform containment analysis without requiring the additional assumptions imposed in existing research.Notably,the IT-2 fuzzy tracking controller can also be applied in collision avoidance situations to track the desired trajectories calculated by the avoidance algorithm under the Artificial Potential Field(APF).Benefiting from the combination of vortex and source APFs,the leaders can properly adjust the system dynamics to prevent potential collision risk.Integrating the fuzzy theory and APFs avoidance algorithm,an IT-2 fuzzy controller design approach is proposed to achieve the F-and-C purposewhile ensuring collision avoidance capability.Finally,amulti-ship simulation is conducted to validate the feasibility and effectiveness of the designed IT-2 fuzzy controller.
基金Supported by National Natural Science Foundation of China,No.82002609.
文摘BACKGROUND The role of NLR family pyrin domain containing 3(NLRP3)in post-endoscopic submucosal dissection(ESD)esophageal stricture remains incompletely understood.The effect of celastrol(CEL)on the prevention of esophageal strictures has not yet been investigated.AIM To explore the effect of CEL on the prevention of esophageal stricture in rats.METHODS NLRP3,interleukin(IL)-1β,and IL-18 mRNA levels were measured in patients’tissues after esophageal ESD.NLRP3 expression in esophageal fibroblasts was determined using immunohistochemistry and immunofluorescence staining.Lentiviral transfection was used to induce NLRP3 overexpression and thioredoxin reductase 1(TXNRD1)silencing.The CCK8 assay was used to determine the optimal CEL concentration.Reactive oxygen species(ROS)generation was detected via fluorescence and flow cytometry.Masson’s trichrome staining and barium esophagography were performed to assess collagen deposition and esophageal stenosis.RESULTS The mRNA levels of NLRP3 and IL-1βwere higher in human tissues from the ESD resection bed than in normal esophageal mucosa.NLRP3 overexpression in primary rat esophageal fibroblasts led to high collagen 1 expression.Thus,NLRP3 participated in esophageal inflammation and tissue repair after ESD.Comparable to prednisolone,CEL significantly inhibited NLRP3 activation in vitro and in vivo,and esophageal strictures were markedly alleviated.Mechanistically,CEL upregulated TXNRD1 expression and reduced ROS production,thereby inhibiting NLRP3 expression.This effect was reversed by TXNRD1 silencing.Furthermore,TXNRD1 interacted with NLRP3 and promoted its ubiquitination.CONCLUSION CEL is a promising alternative therapeutic agent for the prevention of post-ESD esophageal strictures.
基金Supported by General Program of National Natural Science Foundation of China,No.81770197Scientific and Technological Research Major Program of Chongqing Municipal Education Commission,No.KJZD-M202312802+1 种基金Chongqing Natural Science Foundation of China,No.CSTB2022NSCQ-MSX0190,No.CSTB2022NSCQ-MSX0176,and No.cstc2020jcyj-msxmX0051Xinqiao Young Postdoc Talent Incubation Program,No.2022YQB098.
文摘BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.
基金supported by the National Natural Science Foundation of China (Grant No.11902157)Natural Science Foundation of Jiangsu Province (Grant No.BK20180417)the Scientific and Technological Innovation Project of Army Engineering Univeristy of PLA (Grant No.KYGYZXJK150025)。
文摘An innovative multi-layer composite explosion containment vessel(CECV)utilizing a sliding steel platealuminum honeycomb-fiber cloth sandwich is put forward to improve the anti-explosion capacity of a conventional single-layer explosion containment vessel(SECV).Firstly,a series of experiments and finite element(FE)simulations of internal explosions are implemented to understand the basic anti-explosion characteristics of a SECV and the rationality of the computational models and methods is verified by the comparison between the experimental results and simulation results.Based on this,the CECV is designed in detail and a variety of FE simulations are carried out to investigate effects of the sandwich structure,the explosive quantity and the laying mode of the fiber cloth on anti-explosion performance and dynamic response of the CECV under internal explosions.Simulation results indicate that the end cover is the critical position for both the SECV and CECV.The maximum pressure of the explosion shock wave and the maximum strain of the CECV can be extremely declined compared to those of the SECV.As a result,the explosive quantity the CECV can sustain is up to 20 times of that the SECV can sustain.Besides,as the explosive quantity increases,the internal pressure of the CECV keeps growing and the plastic deformation and failure of the sandwich structure become more and more severe,yielding plastic strain of the CECV in addition to elastic strain.The results also reveal that the laying angles of the fiber cloth's five layers have an impact on the anti-explosion performance of the CECV.For example,the CECV with fiber cloth layered in 0°/45°/90°/45°/0°mode has the optimal anti-capacity,compared to 0°/0°/0°/0°/0°and 0°/30°/60°/30°/0°modes.Overall,owing to remarkable anti-explosion capacity,this CECV can be regarded as a promising candidate for explosion resistance.
