[Objective] The study aimed at evaluating the uncertainty in measuring the construct-specific fragments of genetically modified maize MON863 by real time quantitative PCR.[Method] The content of construct-specific fra...[Objective] The study aimed at evaluating the uncertainty in measuring the construct-specific fragments of genetically modified maize MON863 by real time quantitative PCR.[Method] The content of construct-specific fragments in MON863 samples was determined by real time quantitative PCR,and then the uncertainty of measurement result was evaluated according to the sources of uncertainty like the PCR system,the data processing and the micropipette.[Result] Type A evaluation of uncertainty（uA） in the measurement was 1.7×10^-2;Type B evaluation of uncertainty（uB） was 9.0×10^-4;the combined standard uncertainty（uC） was 1.7×10^-2;the expanded uncertainty（U95） was 0.036 and the finally measured result was 1.08%±0.036.[Conclusion] The main uncertainty of the result measured by real time quantitative PCR came from the randomizing effect in the experimental process.展开更多
In the current era where visual media dominate audiences'daily entertainment and other activities,the"fragmented"pattern of information consumption seems to have become the mainstream.However,long-form n...In the current era where visual media dominate audiences'daily entertainment and other activities,the"fragmented"pattern of information consumption seems to have become the mainstream.However,long-form narrative programs in online audio,represented by podcasts and audio dramas,have emerged as a non-negligible communication force.This paper analyzes this form from three aspects:content construction,value dimensions,and cultural significance.In terms of content,it explores the diversity of narrative subjects,the cross-border integration of themes,and the innovation of narrative structures;in terms of value,it elaborates on its role in knowledge dissemination,emotional connection,and preservation of cultural memory;in terms of significance,it reveals its impact on the balance of media ecology,the expansion of public discourse space,and the revival of auditory culture.Long-form narrative content in online audio holds significant contemporary significance that deserves attention.展开更多
基金Supported by the Fund from Sichuan Academy of Agricultural Sciences for Distinguished Young Scholars (2009QNJJ-037)a Grantfor Adventive Species Monitoring from Ministry of Agriculture~~
文摘[Objective] The study aimed at evaluating the uncertainty in measuring the construct-specific fragments of genetically modified maize MON863 by real time quantitative PCR.[Method] The content of construct-specific fragments in MON863 samples was determined by real time quantitative PCR,and then the uncertainty of measurement result was evaluated according to the sources of uncertainty like the PCR system,the data processing and the micropipette.[Result] Type A evaluation of uncertainty（uA） in the measurement was 1.7×10^-2;Type B evaluation of uncertainty（uB） was 9.0×10^-4;the combined standard uncertainty（uC） was 1.7×10^-2;the expanded uncertainty（U95） was 0.036 and the finally measured result was 1.08%±0.036.[Conclusion] The main uncertainty of the result measured by real time quantitative PCR came from the randomizing effect in the experimental process.
文摘In the current era where visual media dominate audiences'daily entertainment and other activities,the"fragmented"pattern of information consumption seems to have become the mainstream.However,long-form narrative programs in online audio,represented by podcasts and audio dramas,have emerged as a non-negligible communication force.This paper analyzes this form from three aspects:content construction,value dimensions,and cultural significance.In terms of content,it explores the diversity of narrative subjects,the cross-border integration of themes,and the innovation of narrative structures;in terms of value,it elaborates on its role in knowledge dissemination,emotional connection,and preservation of cultural memory;in terms of significance,it reveals its impact on the balance of media ecology,the expansion of public discourse space,and the revival of auditory culture.Long-form narrative content in online audio holds significant contemporary significance that deserves attention.
