The discharge of micro-polluted water from sources such as agricultural runoff,urban stormwater,and treated effluents presents significant challenges to aquatic ecosystems.Constructed wetlands(CWs)have gained recog-ni...The discharge of micro-polluted water from sources such as agricultural runoff,urban stormwater,and treated effluents presents significant challenges to aquatic ecosystems.Constructed wetlands(CWs)have gained recog-nition as an eco-friendly solution for removing pollutants from various wastewater sources and are increasingly applied for micro-polluted water treatment.By reviewing 78 full-scale CW studies from Web of Science,it is summarized that the ranges of ammonium nitrogen(NH4+-N)concentrations in runoff,wastewater treatment plant effluent and polluted river were 0.1–6.6,0.3–12.3,and 0.2–41.1 mg/L,respectively.The ranges of ni-trate nitrogen concentrations were 0.2–14.2,0–5.7,and 0–2.6 mg/L,respectively.Removal efficiencies of CWs for micro-polluted water varied by CW types.The total nitrogen removal efficiencies for subsurface-flow CWs,free-water surface-flow CWs,and hybrid CWs ranged from 27.4%to 66.5%,16.8%to 89.8%,and 19.4%to 88.2%,respectively.The NH4+-N removal efficiencies ranged from 34.2%to 73.6%,38.4%to 89.4%and 13.5%to 94.2%,respectively.Additionally,other factors influencing contaminant removal efficiency such as hydraulic retention time,vegetation types,redox micro-environment and influent water quality were evaluated.Based on these findings,two strategies for improving the purification performance of CWs were proposed:the selection of incorporating electron donor substrates and the optimization of operation parameters.This paper serves as a synthesis of information to guide future research and full-scale CW applications in micro-polluted water treatment.展开更多
Anammox bacteria in constructed wetlands(CWs)play pivotal role in sustainable nitrogen transformation,yet existing studies lack comprehensive analysis of environmental gradients and microbial interactions,both key fac...Anammox bacteria in constructed wetlands(CWs)play pivotal role in sustainable nitrogen transformation,yet existing studies lack comprehensive analysis of environmental gradients and microbial interactions,both key factors in anammox bacteria enrichment.This study investigated the mechanisms driving anammox bacteria enrichment in lab-scale simulated CWs treating high-nitrogen wastewater,focusing on bacterial community re-sponses across wetland layers with various strategies,including continuous up-flow influent,nitrogen loading increase,effluent recirculation,intermittent influent,and anammox bacteria inoculation.Results showed that total relative and absolute abundances of anammox bacteria ranged from 0.77%to 12.50%and from 0.13 to 6.46×10^(7) copies/g,respectively.Dissolved oxygen and pH had significant positive correlations with the absolute abundance of anammox bacteria,while organic matter and nitrate negatively impacted their relative abundance.Permutational multivariate analysis of variance indicated that spatial heterogeneity explained more variation in anammox bacteria abundance(43.44%)compared to operational strategies(8.58%).In terms of microbial interactions,60 dominant species exhibited potential correlations with anammox bacteria,comprising 170 interactions(105 positive and 65 negative),which suggested that anammox bacteria generally foster cooperative relationships with dominant bacteria.Notably,significant interspecies interactions were observed between Candidatus Kuenenia(dominant anammox bacteria in CWs)and species within the genera Chitinivibrio-nia and Anaerolineaceae,suggesting that microbial interactions primarily manifest as indirect facilitative effects rather than direct mutualistic relationships.Given that the Normalized Stochasticity Ratio in CWs were<50%,this study inferred that environmental gradients have greater influence on anammox bacteria than microbial interactions.展开更多
There is an urgent need to investigate the distinct performance and mechanism of traditional constructed wetland(CW),microbial fuel cells-constructed wetland(MFC-CW)and direct-enchanced constructed wetland(EC-CW)for a...There is an urgent need to investigate the distinct performance and mechanism of traditional constructed wetland(CW),microbial fuel cells-constructed wetland(MFC-CW)and direct-enchanced constructed wetland(EC-CW)for antibiotics removal.To address this issues,three kinds of CWs were bulit and distinct performance and mechanism for levofloxacin(LVFX)removal was expored in this study.MFC-CW,EC-CW achieved high LVFX removal efficiencies compared with traditional constructed wetland(96.0%in MFC-CW,95.0%in EC-CW and 91.2%in CW)and the biodegradation was the key contributor(90.7%-95.3%).MFC-CW is better capable to stimulate microbial activity and strengthen the removal effect of LVFX.MFC-CW performed well in reducing the effluent's ecotoxicity(dehydrogenase activity)and antibiotic resistance genes(ARGs).ARGs were mainly distributed in the anode region of MFC-CW while accumulating in the cathode region of EC-CW.Atribacteria,Chlorobi,Synergistetes and Firmicutes significicant effect on the efficiencies of TN,NH_(4)^(+),and LVFX.The core node for microbial community interaction in CW was only Pseudomonas in Proteobacteria(OTU4537).While MFC-CW included Opitutae_vadin HA64(OTU3153)in Verrucomicrobia,Desulfomicrobium(OTU3629)in Proteobacteria,and Actinobacteria Gaiellales(OTU4206),and EC-CW included Mesotoga(OTU1104)in Thermotogae,Syntrophus(OTU3207)in Proteobacteria and Emticicia(OTU3788)in Bacteroidetes.MFC-CW and EC-CW increased the abundance of critical microbial communities,and the microbial communities were more closely related.This study improved the understanding of the molecular mechanisms underlying the enhancement of CW by the two bioelectrochemical methods.展开更多
Constructed wetlands(CWs) are a promising method to treat effluent from wastewater treatment plants(WWTPs),However,low carbon/nitrogen(C/N) ratios of the influent inhibit denitrification in CWs,resulting in poor nitro...Constructed wetlands(CWs) are a promising method to treat effluent from wastewater treatment plants(WWTPs),However,low carbon/nitrogen(C/N) ratios of the influent inhibit denitrification in CWs,resulting in poor nitrogen removal efficiency.Herein,we compared traditional(control),biochar(BC), andβ-cyclodextrin-fu nctionalized biochar(BC@β-CD) CW systems to investigate nitrogen removal from influent with low C/N ratios,and the mechanisms that enhance this process.The highest nitrogen removal rates were observed in the BC@β-CD group,with rates 45.89% and 42.48% higher than those of the control,accompanied by a 70.57% and 85.45% decrease in nitrous oxide release,when the C/N ratio decreased from4 to 2,respectively.Metagenomic and enzymatic analyses indicated that BC@β-CD enhances nitrogen removal by coordinately promoting carbon metabolism and increasing denitrification enzyme activities,without affecting microbial species diversity in CWs.Structural equation modeling confirmed that the foremost advantages of BC@β-CD were effective electron generation and transportation resulting from increased activities of nicotinamide adenine dinucleotide(NADH) dehydrogenase and the electron transfer system(ETS),thereby strategically reallocating more carbon metabolic flow to support denitrification.Our results show that the application of BC@β-CD in CWs to optimize the reallocation of electrons from carbon metabolism is a feasible strategy to enhance denitrification under low C/N conditions.展开更多
Constructed Wetlands (CWs) are currently one of the most promising techniques for wastewater treatment, having demonstrated their effectiveness. However, the choice of substrate particle size is critical to the smooth...Constructed Wetlands (CWs) are currently one of the most promising techniques for wastewater treatment, having demonstrated their effectiveness. However, the choice of substrate particle size is critical to the smooth operation of the process, as hydrodynamic constraints require a coarse particle size, whereas wastewater treatment recommends a fine particle size. This study investigates the suitability of laterite and shale as substrates of different sizes (1 - 3, 3 - 5 and 5 - 8 mm) in CWs for domestic wastewater treatment. The study was carried out in an experimental pilot plant consisting of 12 parallelepiped beds (C × C = 0.4 × 0.4 m2;H = 0.6 m) filled from bottom to top with 0.1 m of gravel and 0.4 m of shale or laterite of different grain sizes with two replications. During the six months of operation, plant biomass and stem diameter of Pennisetum purpureum used as vegetation in the CWs were determined. Raw and treated water were also sampled and analyzed for pollutants, including chemical oxygen demand (COD), biochemical oxygen demand (BOD5), total Kjedahl nitrogen (TKN), total phosphorus (TP), and total suspended solids (TSS), using International Organization for Standardization (ISO) analytical methods. P. purpureum developed much better in the CW beds lined with shale;plant biomass ranged from 13.8 to 14.7 kg/m2 and from 11.2 to 12.5 kg/m2 in the beds lined with shale and laterite, respectively, as did stump diameter, which ranged from 15.5 to 16.1 cm and from 11.10 to 12.7 cm, respectively. However, the highest values for biomass and stump diameter for each material were obtained in the beds lined with 1 - 3 mm geomaterials. Pollutant removal efficiencies were highest in the CWs lined with laterite and shale of 1 - 3 mm grain size (76.9% - 83% COD, 78% - 84.7% BOD5, 55.5% - 72.2% TKN, 58.4% - 72.4% TP, 78.1% - 80.2% TSS), with the highest values recorded in the shale-lined beds. However, the 3 - 5 mm grain size of both materials provided quality filtrates (140 - 174 mg/L COD, 78.5 - 94.8 mg/L BOD5, 4.6 - 5.7 mg/L TP) in line with local wastewater discharge levels. This size of geomaterials appears to be suitable for optimization purposes, although further work with these materials, such as increasing the depth of the wetland, is required to improve the level of NTK and TSS discharge.展开更多
Discharge from sewage treatment plants can deteriorate river water quality due to improper management or insufficient levels of treatment.To address these challenges,constructed wetland systems(CWSs)have been widely u...Discharge from sewage treatment plants can deteriorate river water quality due to improper management or insufficient levels of treatment.To address these challenges,constructed wetland systems(CWSs)have been widely used as a sustainable and effective solution for wastewater management.This study provides an overview of the conceptual design for a CWS to treat the discharge from the Papan Regional Sewage Treatment Plant(RSTP).The design influent for the CWS was determined,and the proposed sizing was based on simulation results to achieve the targeted concentrations consistent with Water Quality Index(WQI)Class II.The MUSIC-X software was used in this study to simulate pollutant removal performance,hydraulic behavior,and effluent quality under various flow conditions.The model was set up using site-specific inflow parameters,targeted effluent concentrations,and wetland configuration inputs,including surface area,depth,vegetation zones,and retention times.This study introduces an innovative approach by adopting the paddy field concept as the geometrical design of a multi-cell CWS,covering a total surface area of 1,250,000m^(2)and divided into two sections(Wetland 1 and Wetland 2),with different planting plots separated by a gabion wall.The innovation lies in reimagining abandoned mining ponds as functional wetland zones,while using a paddy field-inspired grid layout to enhance hydraulic control,pollutant removal,and land efficiency.This approach transforms degraded land into an eco-engineered,cost-effective,and scalable wastewater treatment system-a novel model for tropical developing regions.展开更多
Microbial fuel cell(MFC)coupled constructedwetland(CW)is regarded as a promising green technology due to its simultaneous removal performance for the co-occurrence of various contaminants in wastewater.In this study,t...Microbial fuel cell(MFC)coupled constructedwetland(CW)is regarded as a promising green technology due to its simultaneous removal performance for the co-occurrence of various contaminants in wastewater.In this study,the simultaneous removal performance of sulfamethazine(SMZ)and hexavalent chromium Cr(VI)in the CW and MFC-CW systems was investigated.The removal efficiencies of total nitrogen(N),total phosphorus(P),and chemical oxygen demand(COD)were also examined.The results demonstrated that Cr(VI)was effectively eliminated with an excellent removal efficiency of>98.0%,followed by SMZ with a removal efficiency of 70.3%-85.6%.Additionally,during the long-term operation period,the average removal efficiency for N,P,and COD ranged from 74.0%to 96.1%,83.6%to 94.1%,and 91.1%to 95.3%,respectively.The microbial community and antibiotic resistance genes(ARGs)in the anode and cathode were also analyzed separately to evaluate the SMZ and Cr(VI)removal performance of MFC-CW.The abundance of corresponding ARGs was slightly different in the anode and cathode regions.The average abundance of sul4 in the SMZ+Cr(VI)treatment MFC-CW was significantly higher than that of other sul1-3.This study offers valuable insights for the simultaneous removal of SMZ and Cr(VI)from wastewater by MFC-CW.展开更多
A membrane-less constructed wetland microbial fuel cell （CW-MFC） is constructed and operated under continuous flow with a hydraulic retention time （HRT） of 2 d. Fed with glucose, the CW-MFC generates a stable curr...A membrane-less constructed wetland microbial fuel cell （CW-MFC） is constructed and operated under continuous flow with a hydraulic retention time （HRT） of 2 d. Fed with glucose, the CW-MFC generates a stable current density of over 2 A/m3 with a resistor of 1 kΩ and has a chemical oxygen demand （COD） removal efficiency of more than 90% after the startup of 2 to 3 d. A series of systems with the electrode spacings of 10, 20, 30 and 40 cm are compared. It is found that the container with the electrode spacing of 20 cm gains the highest voltage of 560 mV, the highest power density of 0. 149 W/m 3, and the highest Coulombic efficiency of 0.313%. It also has the highest COD removal efficiency of 94. 9%. In addition, the dissolved oxygen （DO） concentrations are observed as the lowest level in the middle of all the CW-MFC reactors. The results show that the more COD is removed, the greater power is generated, and the relatively higher Coulombic efficiency will be achieved. The present study indicates that the CW-MFC process can be used as a cost-effective and environmentally friendly wastewater treatment with simultaneous power generation.展开更多
Constructed wetlands as a wastewater eco-treatment technology has devel-oped for decades. Combining wastewater-treatment with water recycle in an efficient way, it plays an important role in water body restoration and...Constructed wetlands as a wastewater eco-treatment technology has devel-oped for decades. Combining wastewater-treatment with water recycle in an efficient way, it plays an important role in water body restoration and ecological construction. Constructed wetlands with unique advantages have attracted intensive attention since it developed, and have been widely used in treatment of domestic sewage, industrial wastewater, and mine wastewater. In this paper, we summarized the clas-sification, composition, combination, operation mode and pol utant removal mecha-nism of constructed wetlands, as wel as the research progress on the application of constructed wetland in wastewater treatment at home and abroad. In addition, in view of the problems of using constructed wetlands to treat wastewater in China, corresponding solutions were put forward, including setting up system construction standards for constructed wetlands, developing the regulatory and enhancing tech-niques of nitrogen and phosphorus removal, and taking advantage of the unique features of constructed wetlands to improve and restore ecological environment.展开更多
[Objective] The control effect of floating plants constructed wetland to nitrogen and phosphorus pollution from rice field drainage was studied.[Method] Firstly,the characteristic of floating plants and the purificati...[Objective] The control effect of floating plants constructed wetland to nitrogen and phosphorus pollution from rice field drainage was studied.[Method] Firstly,the characteristic of floating plants and the purification principle of pollutants were introduced,and then purification effect was analyzed through controlling nitrogen and phosphorus pollution from rice field drainage by floating plants constructed wetland.[Result] By means of floating plants constructed wetland,the average removal rate of total nitrogen(TN) from double-season early and late rice field drainage reached 52.17% and 62.23%,respectively,while that of total phosphorus(TP) was 45.69% and 74.37%,respectively,with better removal effect.Meanwhile,floating plants have ecological and ornamental value to some extent.[Conclusion] Floating plants constructed wetland could not only control nitrogen and phosphorus pollution in field drainage effectively but also keep farmland ecosystem in healthy state.展开更多
The basic chemistry laboratories of three colleges and universities in Guizhou Province were investigated completely,especially the pollution situations of the basic chemistry projects were counted,and the discharge a...The basic chemistry laboratories of three colleges and universities in Guizhou Province were investigated completely,especially the pollution situations of the basic chemistry projects were counted,and the discharge amounts of the poisonous and harmful pollutants in the waste liquid from laboratories were monitored and analyzed.On the basis of the summing-up and analysis of the current research findings,a simple and feasible treatment scheme through flocculating,precipitating and constructed wetlands was designed to control the three kinds of excessive pollutants.展开更多
In this study, the project of constructed wetland for treatment of tailwater from the wastewater treatment plant in Wudang Mountain was taken as an example, and the technological processes, pollution load, wetland bed...In this study, the project of constructed wetland for treatment of tailwater from the wastewater treatment plant in Wudang Mountain was taken as an example, and the technological processes, pollution load, wetland bed structure, bed filler, selection of plants and hydraulic conditions of the subsurface flow constructed wetland were discussed. A subsurface flow constructed wetland, which covered an area of 7 227 m^2 was finally designed. It could treat 7 000 m^3 of tailwater from the wastewater treatment plant a day. In addition, the system could reduce the emission of COD, BODs, TN, TP and NH3-N by 25.55, 25.55, 12.78, 1.28 and 17.89 t respectively a day. The outlet water was proved to reach the Standard A of the first class in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant （GB 18918-2002）.展开更多
[Objective]The activities of urease and phosphatase in integrated vertical flow constructed wetland and purification effect of black and malodorous river were studied.[Method]Honeycomb stone and soft filler were used ...[Objective]The activities of urease and phosphatase in integrated vertical flow constructed wetland and purification effect of black and malodorous river were studied.[Method]Honeycomb stone and soft filler were used as matrix,the biological grid integrated vertical flow constructed wetland system was built to strengthen treatment for black and malodorous river,and the activities of urease and phosphatase of two fillers in different functional areas of constructed wetland were tested to study the film formation property of two fillers in different functional areas.[Result]Both urease and phosphatase activities on biofilm of soft filler were significantly higher than on biofilm of honeycomb stone filler;indicating that choosing soft filler was more advantageous to the growth of biofilm;and urease activity had significant correlation with total nitrogen removal rate,while phosphatase activity had no significant correlation with total phosphorus removal rate.[Conclusion]This study provided evidences for utilizing the activities of urease and phosphatase to evaluate the purification effect of black and malodorous river and choose appropriate filler.展开更多
[Objective] The aim was to choose appropriate floating plant in the wetland in the north China.[Method] Pistia stratiotes L.,Eichhornia crassipes,and Hydrocharis dubia（Bl.） Backer were planted in the aquatic biologi...[Objective] The aim was to choose appropriate floating plant in the wetland in the north China.[Method] Pistia stratiotes L.,Eichhornia crassipes,and Hydrocharis dubia（Bl.） Backer were planted in the aquatic biological pool of constructed subsurface flow wetland system in the reservoir.Through filed investigation,the growth of the three kinds of plants was studied and their adaptability to the northern climate was concluded.[Result] Judging from the growth speed and state of the three kinds of floating plants,the biological characteristic of Pistia stratiotes L.can perfectly adapt the environment in the pool in the reservoir,followed by the Eichhornia crassipes.The growth state of the Hydrocharis dubia（Bl.） Backer was the worst one and it can not adapt to the north environment.[Conclusion] It provided references for the choice of artificial floating plant in the north area.展开更多
The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied....The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied.The results revealed that,in Summer and Autumn,especially in August,C.lacryma-jobi could obtain higher biomass and N,P accumulations,thereby achieving the most efficient purification; from the breeding value point of view,N,P contents could be improved by the appropriate increase of harvest times of C.lacryma-jobi,while too many harvest times was inappropriate to the wastewater purification by C.lacryma-jobi and the nutrients accumulation.So,C.lacryma-jobi should be harvested completely in August during its growth period,and twice-3 times of harvests was appropriate.展开更多
[Objective] The aim was to explore the distributions of main microorganisms in constructed wetland of Iris pseudacorus L.and their function and position in wastewater treatment.[Method]Distributions of main microorgan...[Objective] The aim was to explore the distributions of main microorganisms in constructed wetland of Iris pseudacorus L.and their function and position in wastewater treatment.[Method]Distributions of main microorganisms in the upstream vertical-flow constructed wetland of Iris pseudacorus was studied by training and observing 4 kinds of microorganisms.[Result]The total number of microorganisms on root surface in unit area was 15.32 times as many as that on filler surfacein,among them bacteria and actinomycete were 17.14 times and 19.84 times as many as those on filler surfacein,respectively.[Conclusion]The distributions of microorganisms are significantly different in different parts of wetland system,obviously on root surface.Bacteria are the main populations of biological wastewater treatment.展开更多
[Objection] The aim was to study application of channels constructed wetland in treatment of domestic sewages in new countryside. [Method] Supported by construction of new rural areas project in China, we surveyed on ...[Objection] The aim was to study application of channels constructed wetland in treatment of domestic sewages in new countryside. [Method] Supported by construction of new rural areas project in China, we surveyed on new rural areas in Gannan, Jiangxi Province, summarized source, characteristics and treatment of domestic sewage in countryside, and designed channels constructed wetland for sewage treatment. [Result] The technique is proven effective in sewage treatment. After the technique was conducted for a trial run for four months, removal rates of COD, TP and TN averaged 73.07%, 73.25% and 72.36%, respectively. After contin- uous sampling for six times, effluent COD was analyzed 20-35 mg/L, TP was 0.60- 1.19 mg/L and TN was 6.88-11.21 mg/L, better than that of Standard 1B ruled by Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant. [Conclusion] Thanks for low investment, channels constructed wetland can be built by trans- formation of bottom land, proving a good way for treatment of diffuse pollution source and control of water non-point pollution.展开更多
[Objective] The aim was to al eviate the constructed wetland clogging problems and to explore to the effects of hydraulic loading on wetland clogging. [Method] The experiment, through artificial soil columns, simulate...[Objective] The aim was to al eviate the constructed wetland clogging problems and to explore to the effects of hydraulic loading on wetland clogging. [Method] The experiment, through artificial soil columns, simulated vertical flow arti-ficial wetland, set four hydraulic load level at 50, 100, 150 and 200 cm/d, to identify the impact of hydraulic loading on wetland clogging and to explore the factors run-ning threshold. [Result] The results show that the different levels of hydraulic loading have greater impact; in the constructed wetland clogging process under high hy-draulic loading of 200 cm/d, the effective life was only six months, and the single factor can be speculated that the threshold of the hydraulic load should be at 100-150 cm/d; system can last for six months at low hydraulic loading and last for three months at medium hydraulic load. [Conclusion] The research provides references for wetland clogging experiments in future.展开更多
cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones,...cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.展开更多
[ Objective] This study was to research the treatment effect of different media in undercurrent wetland on contamination, so as to pro- vide reference for rehabitating water body of river along small towns. [ Method ]...[ Objective] This study was to research the treatment effect of different media in undercurrent wetland on contamination, so as to pro- vide reference for rehabitating water body of river along small towns. [ Method ] Three different media of gravel, cobblestone and shale were used to rehabilitate water quality of contaminated river. [ Result ] Gravel, cobblestone and shale all performed well in removing TN, TP and CODw,, in contaminated water, of which gravel stuffed undercurrent wetland run best, averagely removing 49.4% TN, 34.7% and 48. 5% COD~, respectively. [ Conclusion] Undercurrent constructed wetland can effectively improve the water quality of contaminated river, and it is cheaper in cost and simpler in operation, thus suitable for generalizing in small towns of China.展开更多
基金supported by the Natural Science Foundation of China(No.52470105)the Young Taishan Scholars Program of Shandong Province(No.358202103017).
文摘The discharge of micro-polluted water from sources such as agricultural runoff,urban stormwater,and treated effluents presents significant challenges to aquatic ecosystems.Constructed wetlands(CWs)have gained recog-nition as an eco-friendly solution for removing pollutants from various wastewater sources and are increasingly applied for micro-polluted water treatment.By reviewing 78 full-scale CW studies from Web of Science,it is summarized that the ranges of ammonium nitrogen(NH4+-N)concentrations in runoff,wastewater treatment plant effluent and polluted river were 0.1–6.6,0.3–12.3,and 0.2–41.1 mg/L,respectively.The ranges of ni-trate nitrogen concentrations were 0.2–14.2,0–5.7,and 0–2.6 mg/L,respectively.Removal efficiencies of CWs for micro-polluted water varied by CW types.The total nitrogen removal efficiencies for subsurface-flow CWs,free-water surface-flow CWs,and hybrid CWs ranged from 27.4%to 66.5%,16.8%to 89.8%,and 19.4%to 88.2%,respectively.The NH4+-N removal efficiencies ranged from 34.2%to 73.6%,38.4%to 89.4%and 13.5%to 94.2%,respectively.Additionally,other factors influencing contaminant removal efficiency such as hydraulic retention time,vegetation types,redox micro-environment and influent water quality were evaluated.Based on these findings,two strategies for improving the purification performance of CWs were proposed:the selection of incorporating electron donor substrates and the optimization of operation parameters.This paper serves as a synthesis of information to guide future research and full-scale CW applications in micro-polluted water treatment.
基金supported by Natural Science Foundation of Xiamen,China(No.3502Z20227232)the STS Project of Fujian-CAS(No.2023T3018)Bureau of International Cooperation,Chinese Academy of Sciences(No.322GJHZ2022035MI).
文摘Anammox bacteria in constructed wetlands(CWs)play pivotal role in sustainable nitrogen transformation,yet existing studies lack comprehensive analysis of environmental gradients and microbial interactions,both key factors in anammox bacteria enrichment.This study investigated the mechanisms driving anammox bacteria enrichment in lab-scale simulated CWs treating high-nitrogen wastewater,focusing on bacterial community re-sponses across wetland layers with various strategies,including continuous up-flow influent,nitrogen loading increase,effluent recirculation,intermittent influent,and anammox bacteria inoculation.Results showed that total relative and absolute abundances of anammox bacteria ranged from 0.77%to 12.50%and from 0.13 to 6.46×10^(7) copies/g,respectively.Dissolved oxygen and pH had significant positive correlations with the absolute abundance of anammox bacteria,while organic matter and nitrate negatively impacted their relative abundance.Permutational multivariate analysis of variance indicated that spatial heterogeneity explained more variation in anammox bacteria abundance(43.44%)compared to operational strategies(8.58%).In terms of microbial interactions,60 dominant species exhibited potential correlations with anammox bacteria,comprising 170 interactions(105 positive and 65 negative),which suggested that anammox bacteria generally foster cooperative relationships with dominant bacteria.Notably,significant interspecies interactions were observed between Candidatus Kuenenia(dominant anammox bacteria in CWs)and species within the genera Chitinivibrio-nia and Anaerolineaceae,suggesting that microbial interactions primarily manifest as indirect facilitative effects rather than direct mutualistic relationships.Given that the Normalized Stochasticity Ratio in CWs were<50%,this study inferred that environmental gradients have greater influence on anammox bacteria than microbial interactions.
基金supported by the Regional Cooperation Program of Shanxi Province(No.202304041101039)ICC-CAS(No.SCJC-WRW-2023-18)+1 种基金the Fundamental Research Program of Shanxi Province(No.202203021222404)the Open Research Fund of State Environmental Protection Key Laboratory for Lake Pollution Control(No.2022HPYB-09)。
文摘There is an urgent need to investigate the distinct performance and mechanism of traditional constructed wetland(CW),microbial fuel cells-constructed wetland(MFC-CW)and direct-enchanced constructed wetland(EC-CW)for antibiotics removal.To address this issues,three kinds of CWs were bulit and distinct performance and mechanism for levofloxacin(LVFX)removal was expored in this study.MFC-CW,EC-CW achieved high LVFX removal efficiencies compared with traditional constructed wetland(96.0%in MFC-CW,95.0%in EC-CW and 91.2%in CW)and the biodegradation was the key contributor(90.7%-95.3%).MFC-CW is better capable to stimulate microbial activity and strengthen the removal effect of LVFX.MFC-CW performed well in reducing the effluent's ecotoxicity(dehydrogenase activity)and antibiotic resistance genes(ARGs).ARGs were mainly distributed in the anode region of MFC-CW while accumulating in the cathode region of EC-CW.Atribacteria,Chlorobi,Synergistetes and Firmicutes significicant effect on the efficiencies of TN,NH_(4)^(+),and LVFX.The core node for microbial community interaction in CW was only Pseudomonas in Proteobacteria(OTU4537).While MFC-CW included Opitutae_vadin HA64(OTU3153)in Verrucomicrobia,Desulfomicrobium(OTU3629)in Proteobacteria,and Actinobacteria Gaiellales(OTU4206),and EC-CW included Mesotoga(OTU1104)in Thermotogae,Syntrophus(OTU3207)in Proteobacteria and Emticicia(OTU3788)in Bacteroidetes.MFC-CW and EC-CW increased the abundance of critical microbial communities,and the microbial communities were more closely related.This study improved the understanding of the molecular mechanisms underlying the enhancement of CW by the two bioelectrochemical methods.
基金supported by the National Natural Science Foundation of China(52321005)the Guangdong Basic and Applied Basic Research Foundation(2023A1515012383 and 2024A1515030138)+1 种基金the State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology,2021TS30)the Shenzhen Science and Technology Program(KQTD20190929172630447 and KCXFZ20211020163404007).
文摘Constructed wetlands(CWs) are a promising method to treat effluent from wastewater treatment plants(WWTPs),However,low carbon/nitrogen(C/N) ratios of the influent inhibit denitrification in CWs,resulting in poor nitrogen removal efficiency.Herein,we compared traditional(control),biochar(BC), andβ-cyclodextrin-fu nctionalized biochar(BC@β-CD) CW systems to investigate nitrogen removal from influent with low C/N ratios,and the mechanisms that enhance this process.The highest nitrogen removal rates were observed in the BC@β-CD group,with rates 45.89% and 42.48% higher than those of the control,accompanied by a 70.57% and 85.45% decrease in nitrous oxide release,when the C/N ratio decreased from4 to 2,respectively.Metagenomic and enzymatic analyses indicated that BC@β-CD enhances nitrogen removal by coordinately promoting carbon metabolism and increasing denitrification enzyme activities,without affecting microbial species diversity in CWs.Structural equation modeling confirmed that the foremost advantages of BC@β-CD were effective electron generation and transportation resulting from increased activities of nicotinamide adenine dinucleotide(NADH) dehydrogenase and the electron transfer system(ETS),thereby strategically reallocating more carbon metabolic flow to support denitrification.Our results show that the application of BC@β-CD in CWs to optimize the reallocation of electrons from carbon metabolism is a feasible strategy to enhance denitrification under low C/N conditions.
文摘Constructed Wetlands (CWs) are currently one of the most promising techniques for wastewater treatment, having demonstrated their effectiveness. However, the choice of substrate particle size is critical to the smooth operation of the process, as hydrodynamic constraints require a coarse particle size, whereas wastewater treatment recommends a fine particle size. This study investigates the suitability of laterite and shale as substrates of different sizes (1 - 3, 3 - 5 and 5 - 8 mm) in CWs for domestic wastewater treatment. The study was carried out in an experimental pilot plant consisting of 12 parallelepiped beds (C × C = 0.4 × 0.4 m2;H = 0.6 m) filled from bottom to top with 0.1 m of gravel and 0.4 m of shale or laterite of different grain sizes with two replications. During the six months of operation, plant biomass and stem diameter of Pennisetum purpureum used as vegetation in the CWs were determined. Raw and treated water were also sampled and analyzed for pollutants, including chemical oxygen demand (COD), biochemical oxygen demand (BOD5), total Kjedahl nitrogen (TKN), total phosphorus (TP), and total suspended solids (TSS), using International Organization for Standardization (ISO) analytical methods. P. purpureum developed much better in the CW beds lined with shale;plant biomass ranged from 13.8 to 14.7 kg/m2 and from 11.2 to 12.5 kg/m2 in the beds lined with shale and laterite, respectively, as did stump diameter, which ranged from 15.5 to 16.1 cm and from 11.10 to 12.7 cm, respectively. However, the highest values for biomass and stump diameter for each material were obtained in the beds lined with 1 - 3 mm geomaterials. Pollutant removal efficiencies were highest in the CWs lined with laterite and shale of 1 - 3 mm grain size (76.9% - 83% COD, 78% - 84.7% BOD5, 55.5% - 72.2% TKN, 58.4% - 72.4% TP, 78.1% - 80.2% TSS), with the highest values recorded in the shale-lined beds. However, the 3 - 5 mm grain size of both materials provided quality filtrates (140 - 174 mg/L COD, 78.5 - 94.8 mg/L BOD5, 4.6 - 5.7 mg/L TP) in line with local wastewater discharge levels. This size of geomaterials appears to be suitable for optimization purposes, although further work with these materials, such as increasing the depth of the wetland, is required to improve the level of NTK and TSS discharge.
基金Higher Institution Centre of Excellence Program(HICoE),Grant/Award Number:A305-LRAKH001-0004403901-0000。
文摘Discharge from sewage treatment plants can deteriorate river water quality due to improper management or insufficient levels of treatment.To address these challenges,constructed wetland systems(CWSs)have been widely used as a sustainable and effective solution for wastewater management.This study provides an overview of the conceptual design for a CWS to treat the discharge from the Papan Regional Sewage Treatment Plant(RSTP).The design influent for the CWS was determined,and the proposed sizing was based on simulation results to achieve the targeted concentrations consistent with Water Quality Index(WQI)Class II.The MUSIC-X software was used in this study to simulate pollutant removal performance,hydraulic behavior,and effluent quality under various flow conditions.The model was set up using site-specific inflow parameters,targeted effluent concentrations,and wetland configuration inputs,including surface area,depth,vegetation zones,and retention times.This study introduces an innovative approach by adopting the paddy field concept as the geometrical design of a multi-cell CWS,covering a total surface area of 1,250,000m^(2)and divided into two sections(Wetland 1 and Wetland 2),with different planting plots separated by a gabion wall.The innovation lies in reimagining abandoned mining ponds as functional wetland zones,while using a paddy field-inspired grid layout to enhance hydraulic control,pollutant removal,and land efficiency.This approach transforms degraded land into an eco-engineered,cost-effective,and scalable wastewater treatment system-a novel model for tropical developing regions.
基金supported by the National Natural Science Foundation of China(Nos.51968072 and 52160023)Xinjiang Science Fund for Distinguished Young Scholars(No.2021D01E15)+1 种基金the Tianshan Yingcai-Youth talent support program of Xinjiang(No.2022TSYCCX0010)the“Youth talent support program”of Xinjiang Normal University(No.XJNUQB2022-24).
文摘Microbial fuel cell(MFC)coupled constructedwetland(CW)is regarded as a promising green technology due to its simultaneous removal performance for the co-occurrence of various contaminants in wastewater.In this study,the simultaneous removal performance of sulfamethazine(SMZ)and hexavalent chromium Cr(VI)in the CW and MFC-CW systems was investigated.The removal efficiencies of total nitrogen(N),total phosphorus(P),and chemical oxygen demand(COD)were also examined.The results demonstrated that Cr(VI)was effectively eliminated with an excellent removal efficiency of>98.0%,followed by SMZ with a removal efficiency of 70.3%-85.6%.Additionally,during the long-term operation period,the average removal efficiency for N,P,and COD ranged from 74.0%to 96.1%,83.6%to 94.1%,and 91.1%to 95.3%,respectively.The microbial community and antibiotic resistance genes(ARGs)in the anode and cathode were also analyzed separately to evaluate the SMZ and Cr(VI)removal performance of MFC-CW.The abundance of corresponding ARGs was slightly different in the anode and cathode regions.The average abundance of sul4 in the SMZ+Cr(VI)treatment MFC-CW was significantly higher than that of other sul1-3.This study offers valuable insights for the simultaneous removal of SMZ and Cr(VI)from wastewater by MFC-CW.
基金The Fundamental Research Funds for the Central Universitiesthe National Natural Science Foundation of China (No.51109038)
文摘A membrane-less constructed wetland microbial fuel cell （CW-MFC） is constructed and operated under continuous flow with a hydraulic retention time （HRT） of 2 d. Fed with glucose, the CW-MFC generates a stable current density of over 2 A/m3 with a resistor of 1 kΩ and has a chemical oxygen demand （COD） removal efficiency of more than 90% after the startup of 2 to 3 d. A series of systems with the electrode spacings of 10, 20, 30 and 40 cm are compared. It is found that the container with the electrode spacing of 20 cm gains the highest voltage of 560 mV, the highest power density of 0. 149 W/m 3, and the highest Coulombic efficiency of 0.313%. It also has the highest COD removal efficiency of 94. 9%. In addition, the dissolved oxygen （DO） concentrations are observed as the lowest level in the middle of all the CW-MFC reactors. The results show that the more COD is removed, the greater power is generated, and the relatively higher Coulombic efficiency will be achieved. The present study indicates that the CW-MFC process can be used as a cost-effective and environmentally friendly wastewater treatment with simultaneous power generation.
基金Supported by the National Key Technology R&D Program(2012BAD40B02)~~
文摘Constructed wetlands as a wastewater eco-treatment technology has devel-oped for decades. Combining wastewater-treatment with water recycle in an efficient way, it plays an important role in water body restoration and ecological construction. Constructed wetlands with unique advantages have attracted intensive attention since it developed, and have been widely used in treatment of domestic sewage, industrial wastewater, and mine wastewater. In this paper, we summarized the clas-sification, composition, combination, operation mode and pol utant removal mecha-nism of constructed wetlands, as wel as the research progress on the application of constructed wetland in wastewater treatment at home and abroad. In addition, in view of the problems of using constructed wetlands to treat wastewater in China, corresponding solutions were put forward, including setting up system construction standards for constructed wetlands, developing the regulatory and enhancing tech-niques of nitrogen and phosphorus removal, and taking advantage of the unique features of constructed wetlands to improve and restore ecological environment.
基金Supported by Natural Science Foundation of Guangxi Province(0991026)Foundation of Guangxi Key Laboratory of Environmental Engineering and Protection Assessment(0701K019)+1 种基金Project of Environmental Engineering Innovation Team of Talent Small Upland Construction in Universities in Guangxi([2007]Number71)InnovationProgram Project of Postgraduate Education in Guangxi Province(2009105960815M25)~~
文摘[Objective] The control effect of floating plants constructed wetland to nitrogen and phosphorus pollution from rice field drainage was studied.[Method] Firstly,the characteristic of floating plants and the purification principle of pollutants were introduced,and then purification effect was analyzed through controlling nitrogen and phosphorus pollution from rice field drainage by floating plants constructed wetland.[Result] By means of floating plants constructed wetland,the average removal rate of total nitrogen(TN) from double-season early and late rice field drainage reached 52.17% and 62.23%,respectively,while that of total phosphorus(TP) was 45.69% and 74.37%,respectively,with better removal effect.Meanwhile,floating plants have ecological and ornamental value to some extent.[Conclusion] Floating plants constructed wetland could not only control nitrogen and phosphorus pollution in field drainage effectively but also keep farmland ecosystem in healthy state.
基金Supported by Natural Science Foundation Projects of Education Department of Guizhou Province (2006319)
文摘The basic chemistry laboratories of three colleges and universities in Guizhou Province were investigated completely,especially the pollution situations of the basic chemistry projects were counted,and the discharge amounts of the poisonous and harmful pollutants in the waste liquid from laboratories were monitored and analyzed.On the basis of the summing-up and analysis of the current research findings,a simple and feasible treatment scheme through flocculating,precipitating and constructed wetlands was designed to control the three kinds of excessive pollutants.
文摘In this study, the project of constructed wetland for treatment of tailwater from the wastewater treatment plant in Wudang Mountain was taken as an example, and the technological processes, pollution load, wetland bed structure, bed filler, selection of plants and hydraulic conditions of the subsurface flow constructed wetland were discussed. A subsurface flow constructed wetland, which covered an area of 7 227 m^2 was finally designed. It could treat 7 000 m^3 of tailwater from the wastewater treatment plant a day. In addition, the system could reduce the emission of COD, BODs, TN, TP and NH3-N by 25.55, 25.55, 12.78, 1.28 and 17.89 t respectively a day. The outlet water was proved to reach the Standard A of the first class in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant （GB 18918-2002）.
基金Supported by National Major Science and Technology Projects(2009ZX07317-006)National Major Science and Technology Projects(2009ZX07317-009)~~
文摘[Objective]The activities of urease and phosphatase in integrated vertical flow constructed wetland and purification effect of black and malodorous river were studied.[Method]Honeycomb stone and soft filler were used as matrix,the biological grid integrated vertical flow constructed wetland system was built to strengthen treatment for black and malodorous river,and the activities of urease and phosphatase of two fillers in different functional areas of constructed wetland were tested to study the film formation property of two fillers in different functional areas.[Result]Both urease and phosphatase activities on biofilm of soft filler were significantly higher than on biofilm of honeycomb stone filler;indicating that choosing soft filler was more advantageous to the growth of biofilm;and urease activity had significant correlation with total nitrogen removal rate,while phosphatase activity had no significant correlation with total phosphorus removal rate.[Conclusion]This study provided evidences for utilizing the activities of urease and phosphatase to evaluate the purification effect of black and malodorous river and choose appropriate filler.
基金Supported by National Water Special Project"River Water Environment Comprehensive Management Technology Study and Comprehensive demonstration"(2008ZX07209-002-002)China Institute of Water Resources and Hydropower Research Open Fund(IWHRKF201013)~~
文摘[Objective] The aim was to choose appropriate floating plant in the wetland in the north China.[Method] Pistia stratiotes L.,Eichhornia crassipes,and Hydrocharis dubia（Bl.） Backer were planted in the aquatic biological pool of constructed subsurface flow wetland system in the reservoir.Through filed investigation,the growth of the three kinds of plants was studied and their adaptability to the northern climate was concluded.[Result] Judging from the growth speed and state of the three kinds of floating plants,the biological characteristic of Pistia stratiotes L.can perfectly adapt the environment in the pool in the reservoir,followed by the Eichhornia crassipes.The growth state of the Hydrocharis dubia（Bl.） Backer was the worst one and it can not adapt to the north environment.[Conclusion] It provided references for the choice of artificial floating plant in the north area.
基金Supported by Project Sponsored by National Natural Science Foundation (30370146)Projects Sponsored by European Union (ERBIC18CT960059)~~
文摘The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied.The results revealed that,in Summer and Autumn,especially in August,C.lacryma-jobi could obtain higher biomass and N,P accumulations,thereby achieving the most efficient purification; from the breeding value point of view,N,P contents could be improved by the appropriate increase of harvest times of C.lacryma-jobi,while too many harvest times was inappropriate to the wastewater purification by C.lacryma-jobi and the nutrients accumulation.So,C.lacryma-jobi should be harvested completely in August during its growth period,and twice-3 times of harvests was appropriate.
基金Supported by Research Fund of Jiangsu Construction Office(JS2008JH05)Key Project of Scitechnology Demonstration by Jiangsu Environmental Protection Department(2009023)+1 种基金Natural Science Foundation in Jiangsu(BK2008380)Key Academic Fundfor Young Teachers in Nanjing University of Technology~~
文摘[Objective] The aim was to explore the distributions of main microorganisms in constructed wetland of Iris pseudacorus L.and their function and position in wastewater treatment.[Method]Distributions of main microorganisms in the upstream vertical-flow constructed wetland of Iris pseudacorus was studied by training and observing 4 kinds of microorganisms.[Result]The total number of microorganisms on root surface in unit area was 15.32 times as many as that on filler surfacein,among them bacteria and actinomycete were 17.14 times and 19.84 times as many as those on filler surfacein,respectively.[Conclusion]The distributions of microorganisms are significantly different in different parts of wetland system,obviously on root surface.Bacteria are the main populations of biological wastewater treatment.
基金Supported by Project of the National Eleventh-Five Year Research Program of China(2008BAD96B04)~~
文摘[Objection] The aim was to study application of channels constructed wetland in treatment of domestic sewages in new countryside. [Method] Supported by construction of new rural areas project in China, we surveyed on new rural areas in Gannan, Jiangxi Province, summarized source, characteristics and treatment of domestic sewage in countryside, and designed channels constructed wetland for sewage treatment. [Result] The technique is proven effective in sewage treatment. After the technique was conducted for a trial run for four months, removal rates of COD, TP and TN averaged 73.07%, 73.25% and 72.36%, respectively. After contin- uous sampling for six times, effluent COD was analyzed 20-35 mg/L, TP was 0.60- 1.19 mg/L and TN was 6.88-11.21 mg/L, better than that of Standard 1B ruled by Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant. [Conclusion] Thanks for low investment, channels constructed wetland can be built by trans- formation of bottom land, proving a good way for treatment of diffuse pollution source and control of water non-point pollution.
基金Supported by National Natural Science Foundation of China(41071214)~~
文摘[Objective] The aim was to al eviate the constructed wetland clogging problems and to explore to the effects of hydraulic loading on wetland clogging. [Method] The experiment, through artificial soil columns, simulated vertical flow arti-ficial wetland, set four hydraulic load level at 50, 100, 150 and 200 cm/d, to identify the impact of hydraulic loading on wetland clogging and to explore the factors run-ning threshold. [Result] The results show that the different levels of hydraulic loading have greater impact; in the constructed wetland clogging process under high hy-draulic loading of 200 cm/d, the effective life was only six months, and the single factor can be speculated that the threshold of the hydraulic load should be at 100-150 cm/d; system can last for six months at low hydraulic loading and last for three months at medium hydraulic load. [Conclusion] The research provides references for wetland clogging experiments in future.
文摘cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.
文摘[ Objective] This study was to research the treatment effect of different media in undercurrent wetland on contamination, so as to pro- vide reference for rehabitating water body of river along small towns. [ Method ] Three different media of gravel, cobblestone and shale were used to rehabilitate water quality of contaminated river. [ Result ] Gravel, cobblestone and shale all performed well in removing TN, TP and CODw,, in contaminated water, of which gravel stuffed undercurrent wetland run best, averagely removing 49.4% TN, 34.7% and 48. 5% COD~, respectively. [ Conclusion] Undercurrent constructed wetland can effectively improve the water quality of contaminated river, and it is cheaper in cost and simpler in operation, thus suitable for generalizing in small towns of China.
