Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the...Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the pepper industry by affecting the planting period,growth conditions,and disease susceptibility.The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance.In order to explore the upstream regulatory mechanism of CaABI3/VP1-1,a high-quality standardized yeast hybrid library was successfully constructed for yeast one-,two-,and threehybrid screening using pepper‘ZHC2’as the experimental material,with a library recombinant efficiency of up to 100%.The length of inserted fragments varied from 650 to 5000 bp,the library titer was 5.18×10^(6)colony-forming units(CFU)·mL-1,and the library capacity was 1.04×10^(7)CFU of cDNA inserts.The recombinant bait plasmid was used to successfully identify 78 different proteins through the yeast one-hybrid system,including one transcription factor within the ethylene-responsive factor family and the other within the growth-regulating factor family.The interaction happened between LOC124895848 and CaABI3/VP1-1 promoter by point-to-point yeast one-hybrid experiment.The expression level of the 12 selected protein-coding genes was then evaluated by quantitative real-time polymerase chain reaction.Results indicated the protein coding genes showed different responses to waterlogging stress and that the activity of the CaABI3/VP1-1 promoter could be inhibited or activated by up-regulating or down-regulating gene expression,respectively.The identification of these proteins interacting with the promoter provides a new perspective for understanding the gene regulatory network of hot pepper operating under waterlogging stress and provides theoretical support for further analysis of the complex regulatory relationship between transcription factors and promoters.展开更多
In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH an...In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.展开更多
Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were a...Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.展开更多
Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activ...Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.展开更多
Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar o...Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar of southeastern coast of India in various in vitro systems.Methods:The antioxidant activity was evaluated using different in vitro systems,viz.,I,I-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt(ABTS),H_20_2/H0 radical scavenging,Fe^(2+)ion chelating ability,and reducing potential.Folin-Ciocalteu method was used to determine the total phenolic content of the extracts,and the results were expressed as mg of gallic acid equivalents(GE)/g of the seaweed extracts.Thiobarbituric acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid oxidation.Results:Ethyl acetate(EtOAc)fraction of T.conoides registered significantly higher phenolic content(105.97 mg GE/g)than that of T.ornata(69.63 mg GE/g).Significantly higher antioxidant potential as determined by DPPH(64.14%)radical scavenging activity was registered in EtOAc fraction of T.ornata.A higher ABTS(?)radical scavenging(IC_(50)3.16μg/mL),Fe^(2+)chelating(IC_(50)0.46 mg/mL),H_2O_2 scavenging(IC_(50)4.25 mg/mL),lipid peroxidation inhibitory(TBARS,IC_(50)0.21 mg/mL),and reducing abilities(IC_(50)52.67 mg/mL)(P<0.05)were realized in EtOAc fraction of T.ornata than other fractions.Conclusions:This study indicated the potential use of T.conoides and T.ornata as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption,and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.展开更多
A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual lab...A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual laborer. The dislocation is classified according to Rockwood. Types Ⅰ?and Ⅱ are treated nonoperatively, while types Ⅳ, Ⅴ and Ⅵ are generally treated operatively. Controversy exists regarding the optimal treatment of type Ⅲ dislocations in the high-demand patient. Recent evidence suggests that these should be treated nonoperatively initially. Classic surgical techniques were associated with high complication rates, including recurrent dislocations and hardware breakage. In recent years, many new techniques have been introduced in order to improve the outcomes. Arthroscopic reconstruction or repair techniques have promising short-term results. This article aims to provide a current concepts review on the treatment of AC dislocations with emphasis on recent developments.展开更多
In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,t...In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,the ballistic interaction process was successfully simulated based on the Tuler eButcher and GISSMO coupling failure model.The simulated conoidal fracture morphology was in good agreement with the three-dimensional industrial-computed-tomography image.Further,three main damage zones(zones I,II,and III)were identified besides the crater area,which are located respectively near the crater area,at the back of the target plate,and directly below the crater area.Under the high-speed-impact conditions,in zone II,cracks began to form at the end of the period of crack formation in zone I,but crack formation in zone III started before the end of crack formation in zone II.Further,the damage mechanism differed for different stress states.The microcracks in zone I were formed both by void connection and shear deformation.In the formation of zone I,the stress triaxiality ranged from2.0 to1.0,and the shear failure mechanism played a dominant role.The microcracks in zone II showed the combined features of shear deformation and void connection,and during the formation process,the stress triaxiality was between 0 and 0.5 with a mixed failure mode.Further,the microcracks in zone III showed obvious characteristics of void connection caused by local melting.During the zone III formation,the triaxiality was 1.0e1.9,and the ductile fracture mechanism was dominant,which also reflects the phenomenon of spallation.展开更多
The purpose of this study is to develop a mathematical model of the spiral basilar membrane in the center of the cochlea, which plays an important role in the mammalian auditory system. The basilar membrane transmits ...The purpose of this study is to develop a mathematical model of the spiral basilar membrane in the center of the cochlea, which plays an important role in the mammalian auditory system. The basilar membrane transmits sound vibrations, which are converted into electrical potential changes by the inner hair cells. The basilar membrane is thought to lie on a locally undistorted curved surface because the inner hair cells, which are arranged in an orderly fashion on the basilar membrane, respond to their location-specific frequencies. In mammals, the number of rotations of this surface and the rate of change of its width with each rotation are different. It turns out that by modifying the right helicoid, we can obtain a mathematical model that satisfies these points. In conclusion, even though the three-dimensional structure of the basilar membrane varies among species, this model can reproduce this structure. This further suggests that there are common genetic determinants of cochlear development in mammals. From a practical standpoint, this may be useful for creating cochlear implants.展开更多
In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with ...In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with (α, β)-metrics are given. Last, the Gauss curvature of the conoid in the 3-dimension Randers-Minkowski space is studied.展开更多
基金funded by the National Natural Science Foundation of China(grant no.32260760)the Science and Technology Program of Guizhou Province(grant no.20201Z002)the Platform Construction Project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province(Qianjiaoji[2022]No.040).
文摘Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the pepper industry by affecting the planting period,growth conditions,and disease susceptibility.The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance.In order to explore the upstream regulatory mechanism of CaABI3/VP1-1,a high-quality standardized yeast hybrid library was successfully constructed for yeast one-,two-,and threehybrid screening using pepper‘ZHC2’as the experimental material,with a library recombinant efficiency of up to 100%.The length of inserted fragments varied from 650 to 5000 bp,the library titer was 5.18×10^(6)colony-forming units(CFU)·mL-1,and the library capacity was 1.04×10^(7)CFU of cDNA inserts.The recombinant bait plasmid was used to successfully identify 78 different proteins through the yeast one-hybrid system,including one transcription factor within the ethylene-responsive factor family and the other within the growth-regulating factor family.The interaction happened between LOC124895848 and CaABI3/VP1-1 promoter by point-to-point yeast one-hybrid experiment.The expression level of the 12 selected protein-coding genes was then evaluated by quantitative real-time polymerase chain reaction.Results indicated the protein coding genes showed different responses to waterlogging stress and that the activity of the CaABI3/VP1-1 promoter could be inhibited or activated by up-regulating or down-regulating gene expression,respectively.The identification of these proteins interacting with the promoter provides a new perspective for understanding the gene regulatory network of hot pepper operating under waterlogging stress and provides theoretical support for further analysis of the complex regulatory relationship between transcription factors and promoters.
文摘In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.
基金Supported by Ministry of Education(MOE)of Malaysia and Research Intensive Faculty(RIF)of University of Technology MARA(UiTM)Malaysia[Grant No.600-RMI/DANA 5/3/RIF(185/2012)].
文摘Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.
基金Supported by University Grants Commission,New Delhi.India
文摘Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.
基金Supported by the funding under the Science and Engineering Research Council(SERC)Scheme(SR/FTP/CS-63/2007)from Department of Science and Technology.New Delhi.India
文摘Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(T.conoides)and Turbinaria ornata(T.ornata)collected from Gulf of Mannar of southeastern coast of India in various in vitro systems.Methods:The antioxidant activity was evaluated using different in vitro systems,viz.,I,I-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt(ABTS),H_20_2/H0 radical scavenging,Fe^(2+)ion chelating ability,and reducing potential.Folin-Ciocalteu method was used to determine the total phenolic content of the extracts,and the results were expressed as mg of gallic acid equivalents(GE)/g of the seaweed extracts.Thiobarbituric acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid oxidation.Results:Ethyl acetate(EtOAc)fraction of T.conoides registered significantly higher phenolic content(105.97 mg GE/g)than that of T.ornata(69.63 mg GE/g).Significantly higher antioxidant potential as determined by DPPH(64.14%)radical scavenging activity was registered in EtOAc fraction of T.ornata.A higher ABTS(?)radical scavenging(IC_(50)3.16μg/mL),Fe^(2+)chelating(IC_(50)0.46 mg/mL),H_2O_2 scavenging(IC_(50)4.25 mg/mL),lipid peroxidation inhibitory(TBARS,IC_(50)0.21 mg/mL),and reducing abilities(IC_(50)52.67 mg/mL)(P<0.05)were realized in EtOAc fraction of T.ornata than other fractions.Conclusions:This study indicated the potential use of T.conoides and T.ornata as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption,and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.
文摘A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual laborer. The dislocation is classified according to Rockwood. Types Ⅰ?and Ⅱ are treated nonoperatively, while types Ⅳ, Ⅴ and Ⅵ are generally treated operatively. Controversy exists regarding the optimal treatment of type Ⅲ dislocations in the high-demand patient. Recent evidence suggests that these should be treated nonoperatively initially. Classic surgical techniques were associated with high complication rates, including recurrent dislocations and hardware breakage. In recent years, many new techniques have been introduced in order to improve the outcomes. Arthroscopic reconstruction or repair techniques have promising short-term results. This article aims to provide a current concepts review on the treatment of AC dislocations with emphasis on recent developments.
基金This work was supported by the National Natural Science Foundation of China(Grant No.51571031).
文摘In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,the ballistic interaction process was successfully simulated based on the Tuler eButcher and GISSMO coupling failure model.The simulated conoidal fracture morphology was in good agreement with the three-dimensional industrial-computed-tomography image.Further,three main damage zones(zones I,II,and III)were identified besides the crater area,which are located respectively near the crater area,at the back of the target plate,and directly below the crater area.Under the high-speed-impact conditions,in zone II,cracks began to form at the end of the period of crack formation in zone I,but crack formation in zone III started before the end of crack formation in zone II.Further,the damage mechanism differed for different stress states.The microcracks in zone I were formed both by void connection and shear deformation.In the formation of zone I,the stress triaxiality ranged from2.0 to1.0,and the shear failure mechanism played a dominant role.The microcracks in zone II showed the combined features of shear deformation and void connection,and during the formation process,the stress triaxiality was between 0 and 0.5 with a mixed failure mode.Further,the microcracks in zone III showed obvious characteristics of void connection caused by local melting.During the zone III formation,the triaxiality was 1.0e1.9,and the ductile fracture mechanism was dominant,which also reflects the phenomenon of spallation.
文摘The purpose of this study is to develop a mathematical model of the spiral basilar membrane in the center of the cochlea, which plays an important role in the mammalian auditory system. The basilar membrane transmits sound vibrations, which are converted into electrical potential changes by the inner hair cells. The basilar membrane is thought to lie on a locally undistorted curved surface because the inner hair cells, which are arranged in an orderly fashion on the basilar membrane, respond to their location-specific frequencies. In mammals, the number of rotations of this surface and the rate of change of its width with each rotation are different. It turns out that by modifying the right helicoid, we can obtain a mathematical model that satisfies these points. In conclusion, even though the three-dimensional structure of the basilar membrane varies among species, this model can reproduce this structure. This further suggests that there are common genetic determinants of cochlear development in mammals. From a practical standpoint, this may be useful for creating cochlear implants.
文摘In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with (α, β)-metrics are given. Last, the Gauss curvature of the conoid in the 3-dimension Randers-Minkowski space is studied.
