[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentr...[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. [Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. [Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided.展开更多
The kinetic energy variations of mean flow and turbulence at three levels in the surface layer were calculated by using eddy covariance data from observations at Jinta oasis in 2005 summer. It is found that when the m...The kinetic energy variations of mean flow and turbulence at three levels in the surface layer were calculated by using eddy covariance data from observations at Jinta oasis in 2005 summer. It is found that when the mean horizontal flow was stronger, the turbulent kinetic energy was increased at all levels, as well as the downward mean wind at the middle level. Since the mean vertical flow on the top and bottom were both negligible at that time, there was a secondary circulation with convergence in the upper half and divergence in the lower half of the column. After consideration of energy conversion, it was found that the interaction between turbulence and the secondary circulation caused the intensification of each other. The interaction reflected positive feedback between turbulence and the vertical shear of the mean flow. Turbulent sensible and latent heat flux anomaly were also analyzed. The results show that in both daytime and at night, when the surface layer turbulence was intensified as a result of strengthened mean flow, the sensible heat flux was decreased while the latent heat flux was increased. Both anomalous fluxes contributed to the cold island effect and the moisture island effect of the oasis.展开更多
基金Supported by Science and Technical Development Plan of Jilin City(2013210029)Fund for Supporting Key Subjects in Jilin Agricultural Science and Technology College(2013x023)~~
文摘[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. [Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. [Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided.
基金supported by the State Key Program of National Natural Science of China(Grant Nos.40233035 and 40633014)funded by one of National Basic Research Program of China(Grant No.2009CB421402)
文摘The kinetic energy variations of mean flow and turbulence at three levels in the surface layer were calculated by using eddy covariance data from observations at Jinta oasis in 2005 summer. It is found that when the mean horizontal flow was stronger, the turbulent kinetic energy was increased at all levels, as well as the downward mean wind at the middle level. Since the mean vertical flow on the top and bottom were both negligible at that time, there was a secondary circulation with convergence in the upper half and divergence in the lower half of the column. After consideration of energy conversion, it was found that the interaction between turbulence and the secondary circulation caused the intensification of each other. The interaction reflected positive feedback between turbulence and the vertical shear of the mean flow. Turbulent sensible and latent heat flux anomaly were also analyzed. The results show that in both daytime and at night, when the surface layer turbulence was intensified as a result of strengthened mean flow, the sensible heat flux was decreased while the latent heat flux was increased. Both anomalous fluxes contributed to the cold island effect and the moisture island effect of the oasis.
