Lead (Pb) inhibited the activities of Na+ -K+ ATPase (IC50= 2.0×10^(-6) M), K + -Para-Nitrophenyl phosphatase (PNPPase) (IC50= 3.5×10^(-6) M) and [3H]-ouabain binding (IC50 = 4.0×10^(-5) M) in rat brain...Lead (Pb) inhibited the activities of Na+ -K+ ATPase (IC50= 2.0×10^(-6) M), K + -Para-Nitrophenyl phosphatase (PNPPase) (IC50= 3.5×10^(-6) M) and [3H]-ouabain binding (IC50 = 4.0×10^(-5) M) in rat brain P2 fraction. A variable temperature or pH significantly elevated the inhibition of Na+-K+ ATPase by Pb in buffered acidic, neutral and alkaline pH ranges. Noncompetitive inhibition with respect to activation of Na+ -K+ ATPase by ATP was indicated by a variation in Vmax values with no significant changes in Km values at any temperature studied. In the presence of Pb, for Na+ -K+ ATPase at pH 6.5 and 8.5, Vmax was decreased with an increase in Km values suggesting a mixed type of inhibition. Sulfhydryl agents such as dithiothreitol (DTT) and cvsteine (Cyst), but not glutathione (GSH) offered varied levels of protection against Pb-inhibition of Na + -K+ ATPase at pH 7.5 and 8.5. The present data suggest that inhibition of Na+ -K+ ATPase by Pb is both temperature and pH-dependent. These results also indicate that Pb inhibited Na + -K + ATPase by interfering with phosphorylation of enzyme molecule and dephosphorylation of the enzyme-phosphoryl complex and exerted an effect similar to that of SH-blocking agents.展开更多
目的:探讨复方脑肽节苷脂注射液治疗创伤性骨折周围神经损伤的效果。方法:纳入2020年8月—2022年12月107例创伤性骨折周围神经损伤患者,以随机数表法分为对照组(n=53)和观察组(n=54)。对照组口服甲钴胺片治疗,观察组在对照组基础上加用...目的:探讨复方脑肽节苷脂注射液治疗创伤性骨折周围神经损伤的效果。方法:纳入2020年8月—2022年12月107例创伤性骨折周围神经损伤患者,以随机数表法分为对照组(n=53)和观察组(n=54)。对照组口服甲钴胺片治疗,观察组在对照组基础上加用复方脑肽节苷脂注射液治疗,两组均治疗2周。比较患者住院时间、治疗前后神经电生理指标[运动神经传导速度(MNCV)、感觉神经传导速度(SNCV)]、神经营养因子[睫状神经营养因子(CNTF)、脑源性神经营养因子(BDNF)]、核转录因子κB(NF-κB)、肿瘤坏死因子(TNF-α)、用药不良反应。结果:观察组住院时间短于对照组[(18.83±3.42) d vs (20.75±4.06) d](P<0.05)。治疗前后MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的主效应差异有统计学意义(P<0.05);不考虑测量时间,两组间MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的主效应差异有统计学意义(P<0.05);MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的时间点与组间存在交互作用(P<0.05),两组治疗前后MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的变化幅度不同,观察组变化更明显(P<0.05)。两组用药不良反应比较,差异均无统计学意义(P>0.05)。结论:复方脑肽节苷脂注射液治疗创伤性骨折周围神经损伤安全有效,可加快运动及感觉神经传导速度,促进患者尽快恢复,其机制可能与上调CNTF、BDNF及下调NF-κB、TNF-α水平有关。展开更多
文摘Lead (Pb) inhibited the activities of Na+ -K+ ATPase (IC50= 2.0×10^(-6) M), K + -Para-Nitrophenyl phosphatase (PNPPase) (IC50= 3.5×10^(-6) M) and [3H]-ouabain binding (IC50 = 4.0×10^(-5) M) in rat brain P2 fraction. A variable temperature or pH significantly elevated the inhibition of Na+-K+ ATPase by Pb in buffered acidic, neutral and alkaline pH ranges. Noncompetitive inhibition with respect to activation of Na+ -K+ ATPase by ATP was indicated by a variation in Vmax values with no significant changes in Km values at any temperature studied. In the presence of Pb, for Na+ -K+ ATPase at pH 6.5 and 8.5, Vmax was decreased with an increase in Km values suggesting a mixed type of inhibition. Sulfhydryl agents such as dithiothreitol (DTT) and cvsteine (Cyst), but not glutathione (GSH) offered varied levels of protection against Pb-inhibition of Na + -K+ ATPase at pH 7.5 and 8.5. The present data suggest that inhibition of Na+ -K+ ATPase by Pb is both temperature and pH-dependent. These results also indicate that Pb inhibited Na + -K + ATPase by interfering with phosphorylation of enzyme molecule and dephosphorylation of the enzyme-phosphoryl complex and exerted an effect similar to that of SH-blocking agents.
文摘目的:探讨复方脑肽节苷脂注射液治疗创伤性骨折周围神经损伤的效果。方法:纳入2020年8月—2022年12月107例创伤性骨折周围神经损伤患者,以随机数表法分为对照组(n=53)和观察组(n=54)。对照组口服甲钴胺片治疗,观察组在对照组基础上加用复方脑肽节苷脂注射液治疗,两组均治疗2周。比较患者住院时间、治疗前后神经电生理指标[运动神经传导速度(MNCV)、感觉神经传导速度(SNCV)]、神经营养因子[睫状神经营养因子(CNTF)、脑源性神经营养因子(BDNF)]、核转录因子κB(NF-κB)、肿瘤坏死因子(TNF-α)、用药不良反应。结果:观察组住院时间短于对照组[(18.83±3.42) d vs (20.75±4.06) d](P<0.05)。治疗前后MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的主效应差异有统计学意义(P<0.05);不考虑测量时间,两组间MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的主效应差异有统计学意义(P<0.05);MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的时间点与组间存在交互作用(P<0.05),两组治疗前后MNCV、SNCV、CNTF、BDNF、NF-κB、TNF-α的变化幅度不同,观察组变化更明显(P<0.05)。两组用药不良反应比较,差异均无统计学意义(P>0.05)。结论:复方脑肽节苷脂注射液治疗创伤性骨折周围神经损伤安全有效,可加快运动及感觉神经传导速度,促进患者尽快恢复,其机制可能与上调CNTF、BDNF及下调NF-κB、TNF-α水平有关。
