Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica par...Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica particles employing covalent attachment by EDC/NHS chemistry and via electrostatic interaction.The resulting formulations were examined for the morphology(SEM),chemical composition(FTIR,XPS)as well as protein content.To facilitate the injectability and support the bone regeneration,developed particles were dispersed in biopolymeric sol composed of collagen,chitosan and lysinemodifed hyaluronic acid and crosslinked with genipin.By means of rheological study,the sol-gel in situ transition of obtained systems was verifed.It was found in vitro study that MG-63 cells cultured on the developed composites exhibit signifcantly higher alkaline phosphatase(ALP)activity,compared to the pristine hydrogel.Furthermore,the biomineralization ability in the simulated body fluid(SBF)model was also demonstrated.Our fndings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects,especially as a less-cost effective support/alternative for BMP-2 systems.展开更多
Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were as...Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...展开更多
Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the chang...Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.Results: The D0 (the inverse of the slope of the survival curve) and SF2 (survival fraction at 2 Gy) of HL60 cell line were decreased from 1. 53, 0. 34 to 0. 93, 0. 12, respectively and apopotosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5. 89 ± 2. 91 vs 12. 05 ± 3. 06). Conclusion: CSMI could obviously enhance the radiosensitivity of leukemia cells.Original article on CJIM(Chin) 1998; 18(5): 279展开更多
基金the financial support of National Science Centre,Poland,Grant 2016/21/D/ST5/01635。
文摘Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica particles employing covalent attachment by EDC/NHS chemistry and via electrostatic interaction.The resulting formulations were examined for the morphology(SEM),chemical composition(FTIR,XPS)as well as protein content.To facilitate the injectability and support the bone regeneration,developed particles were dispersed in biopolymeric sol composed of collagen,chitosan and lysinemodifed hyaluronic acid and crosslinked with genipin.By means of rheological study,the sol-gel in situ transition of obtained systems was verifed.It was found in vitro study that MG-63 cells cultured on the developed composites exhibit signifcantly higher alkaline phosphatase(ALP)activity,compared to the pristine hydrogel.Furthermore,the biomineralization ability in the simulated body fluid(SBF)model was also demonstrated.Our fndings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects,especially as a less-cost effective support/alternative for BMP-2 systems.
文摘Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...
文摘Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.Results: The D0 (the inverse of the slope of the survival curve) and SF2 (survival fraction at 2 Gy) of HL60 cell line were decreased from 1. 53, 0. 34 to 0. 93, 0. 12, respectively and apopotosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5. 89 ± 2. 91 vs 12. 05 ± 3. 06). Conclusion: CSMI could obviously enhance the radiosensitivity of leukemia cells.Original article on CJIM(Chin) 1998; 18(5): 279
