Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundan...Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.展开更多
基金supported by the National Natural Science Foundation of China(No.42007128)the Fundamental Research Funds for the Central Universities(No.2024QNYL30)the Graduate Research and Practice Projects of Minzu University of China(No.SZKY2024034).
文摘Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.
