Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to ...Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to address these challenges.By immersing a pre-deposited PD coating in a strong alkaline solution containing poly(allylamine)hydrochloride(PAH),the dissociated PD oligomers undergo covalent crosslinking in situ,leading to the formation of a reconstructed PDPA coating enriched with stable amino groups through thorough crosslinking.The PDPA coating demonstrates superior chemical and mechanical stability compared to PD,while enhancing multifunctional properties and offering improved surface functional modification potential.The PDPA coating holds promise in materials science,biomedical engineering,and nanotechnology,enabling versatile surface modification and functionalization in extreme conditions.展开更多
With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network str...With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network stream to perform packet processing at a semantic level above the network layer. This paper presents an efficient TCP stream reassembly mechanism for real-time processing of high-speed network traffic. By analyzing the characteristics of network stream in high-speed network and TCP connection establishment process, several polices for designing the reassembly mechanism are built. Then, the reassembly implementation is elaborated in accordance with the policies. Finally, the reassembly mechanism is compared with the traditional reassembly mechanism by the network traffic captured in a typical gigabit gateway. Experiment results illustrate that the reassembly mechanism is efficient and can satisfy the real-time property requirement of traffic analysis system in high-speed network.展开更多
Herein, we report a "dissolution-reassembly" approach for preparation of N-doped hollow carbon spheres with mesoporous/microporous composite structure (H(Micro/Meso)CS). Basing on the compositional inhomogen...Herein, we report a "dissolution-reassembly" approach for preparation of N-doped hollow carbon spheres with mesoporous/microporous composite structure (H(Micro/Meso)CS). Basing on the compositional inhomogeneity inside the nanospheres of 3-aminophenol/formaldehyde (3-AF) polymerization, the internal 3-AF oligomer with low-molecular-weight is dissolved by acetone to form cavity. Then the dispersive 3-AF oligomer reassemble with silica oligomer as pore-forming agent and cetyltrimethyl-ammonium bromide as surfactant to form a mesoporous outer shell. In addition, the amount of acetone has a great influence on the morphology and structure of H(Micro/Meso)CS. The obtained H(Micro/Meso)CS shows uniform spherical and microporous/mesoporous shell structure and has a high specific capacity and excellent high rate capability.展开更多
Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a d...Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a dense network of cytoskeletal proteins,cell adhesion molecules,and voltage gated ion channels and is located at the proximal most region of the axon（Koleand Stuart, 2012）.展开更多
Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here...Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here,we develop a high-performance regenerated cellulose separator with nano-cracked structures for SCs via a binary solvent of superbase-derived ionic liquid and dimethylsulfoxide(DMSO).The unique nano-cracks with an average width of 7.45 nm arise from the acceleration of cellulose molecular reassembly by DMSO-regulated hydrogen bonding,which endows the separator with high porosity(70.2%)and excellent electrolyte retention(329%).The outstanding thermal stability(273℃)and mechanical strength(70 MPa)enable the separator to maintain its structural integrity under high temperatures and external forces.With these benefits,the SC utilizing the cellulose separator enables a high specific capacitance of 93.6 F g^(−1) at 1.0 A g^(−1) and a remarkable capacitance retention of 99.5%after 10,000 cycles compared with the commercial NKK-MPF30AC and NKK-TF4030.The robust and high-wettability cellulose separator holds promise as a superior alternative to commercial separators for advanced SCs with enhanced performance and improved safety.展开更多
Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembl...Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10μm by dehydration-rehydration procedure. The giant liposomes were used to study the Cl^- channel activities by patch-clamp technique.Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,(117.0±5.7) pS and (144.7±4.5) pS, respectively, were detected. Their activities were voltage-dependent and allwere blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependentmanner. By constructing the open and close dwell time distribution histograms and then fitting them with exponentialfunction, two time constants were obtained in both the open and the close states. The burst activity and conductancesubstate of the channels were observed. Conclusion; There exist three kinds of Cl^- channels with different conduc-tance in human sperm membrane at least. (Asian J Androl 2001 Sep; 3: 185 - 191)展开更多
In a recent study published in Nature,Huang,Wong and their colleagues identified G2E3 as the major histone H3 lysine 14 mono-ubiquitin(H3K14ub)ligase in mammalian cells.1 By coupling G2E3-dependent H3K14 ubiquitinatio...In a recent study published in Nature,Huang,Wong and their colleagues identified G2E3 as the major histone H3 lysine 14 mono-ubiquitin(H3K14ub)ligase in mammalian cells.1 By coupling G2E3-dependent H3K14 ubiquitination to the recruitment of SUV39H at pericentromeric chromatin,the authors define a conserved pathway that guides the reassembly of H3K9me3 domains after mitosis and secures the proper compartmentalization of chromatin.展开更多
针对输电线路耐张线夹X射线数字成像(X-ray digital radiography, X-DR)图像检测效率较低,且人工识别易受主观因素影响的问题,提出一种基于YOLO-ISC的输电线路耐张线夹压接缺陷检测方法。首先,在YOLOv8的主干网络中引入注意力特征融合(i...针对输电线路耐张线夹X射线数字成像(X-ray digital radiography, X-DR)图像检测效率较低,且人工识别易受主观因素影响的问题,提出一种基于YOLO-ISC的输电线路耐张线夹压接缺陷检测方法。首先,在YOLOv8的主干网络中引入注意力特征融合(iterative attention feature fusion, iAFF)模块,通过逐层融合不同尺度的特征减少信息的丢失;其次,采用SimAM注意力机制、内容感知特征重组算子(content-aware reassembly of features, CARAFE)构建融合网络PANet-SC,增强了缺陷特征的表达能力;最后,将融合后的特征输入检测头YOLO Head进行分类预测。实验结果表明,所用YOLO-ISC模型在检测耐张线夹X-DR图像的平均检测精度(mean average precision, mAP)值达到92.49%,检测速度为23帧/s。针对某类缺陷检测精度不足的问题,讨论模型置信度阈值对实际检测结果的影响,降低模型的误检率。将检测结果与SSD、Faster RCNN、DETR、YOLOv8等算法进行比较,验证所用方法的有效性。展开更多
基金supported by the National Natural Science Foundation of China(Nos.82301156,32371377,and 52203131)Leading Talent Project of Guangzhou Development District(No.2020L013)+1 种基金the Zhejiang Provincial Natural Science Foundation of China(No.LTGY23H140001)the Fundamental Research Funds for Wenzhou Medical University(No.KYYW202208)。
文摘Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to address these challenges.By immersing a pre-deposited PD coating in a strong alkaline solution containing poly(allylamine)hydrochloride(PAH),the dissociated PD oligomers undergo covalent crosslinking in situ,leading to the formation of a reconstructed PDPA coating enriched with stable amino groups through thorough crosslinking.The PDPA coating demonstrates superior chemical and mechanical stability compared to PD,while enhancing multifunctional properties and offering improved surface functional modification potential.The PDPA coating holds promise in materials science,biomedical engineering,and nanotechnology,enabling versatile surface modification and functionalization in extreme conditions.
基金National High-Tech Research and Development Program of China (863 Program) (No.2007AA01Z309)
文摘With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network stream to perform packet processing at a semantic level above the network layer. This paper presents an efficient TCP stream reassembly mechanism for real-time processing of high-speed network traffic. By analyzing the characteristics of network stream in high-speed network and TCP connection establishment process, several polices for designing the reassembly mechanism are built. Then, the reassembly implementation is elaborated in accordance with the policies. Finally, the reassembly mechanism is compared with the traditional reassembly mechanism by the network traffic captured in a typical gigabit gateway. Experiment results illustrate that the reassembly mechanism is efficient and can satisfy the real-time property requirement of traffic analysis system in high-speed network.
基金the National Natural Science Foundation of China(No. 21676070)Hebei One Hundred-Excellent Innovative Talent Program(Ⅲ)(No. SLRC2017034)Beijing National Laboratory for Molecular Sciences
文摘Herein, we report a "dissolution-reassembly" approach for preparation of N-doped hollow carbon spheres with mesoporous/microporous composite structure (H(Micro/Meso)CS). Basing on the compositional inhomogeneity inside the nanospheres of 3-aminophenol/formaldehyde (3-AF) polymerization, the internal 3-AF oligomer with low-molecular-weight is dissolved by acetone to form cavity. Then the dispersive 3-AF oligomer reassemble with silica oligomer as pore-forming agent and cetyltrimethyl-ammonium bromide as surfactant to form a mesoporous outer shell. In addition, the amount of acetone has a great influence on the morphology and structure of H(Micro/Meso)CS. The obtained H(Micro/Meso)CS shows uniform spherical and microporous/mesoporous shell structure and has a high specific capacity and excellent high rate capability.
基金supported by National Institutes of Health Grants NS069688 and NS044916, TIRR Foundationthe Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
文摘Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a dense network of cytoskeletal proteins,cell adhesion molecules,and voltage gated ion channels and is located at the proximal most region of the axon（Koleand Stuart, 2012）.
基金supported by the National Natural Science Foundation of China(No.U22A20422,22178028)the Program of Introducing Talents of Discipline to Universities(Project 111,B21022)the 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University(No.BLRC2023B01)。
文摘Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here,we develop a high-performance regenerated cellulose separator with nano-cracked structures for SCs via a binary solvent of superbase-derived ionic liquid and dimethylsulfoxide(DMSO).The unique nano-cracks with an average width of 7.45 nm arise from the acceleration of cellulose molecular reassembly by DMSO-regulated hydrogen bonding,which endows the separator with high porosity(70.2%)and excellent electrolyte retention(329%).The outstanding thermal stability(273℃)and mechanical strength(70 MPa)enable the separator to maintain its structural integrity under high temperatures and external forces.With these benefits,the SC utilizing the cellulose separator enables a high specific capacitance of 93.6 F g^(−1) at 1.0 A g^(−1) and a remarkable capacitance retention of 99.5%after 10,000 cycles compared with the commercial NKK-MPF30AC and NKK-TF4030.The robust and high-wettability cellulose separator holds promise as a superior alternative to commercial separators for advanced SCs with enhanced performance and improved safety.
文摘Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10μm by dehydration-rehydration procedure. The giant liposomes were used to study the Cl^- channel activities by patch-clamp technique.Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,(117.0±5.7) pS and (144.7±4.5) pS, respectively, were detected. Their activities were voltage-dependent and allwere blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependentmanner. By constructing the open and close dwell time distribution histograms and then fitting them with exponentialfunction, two time constants were obtained in both the open and the close states. The burst activity and conductancesubstate of the channels were observed. Conclusion; There exist three kinds of Cl^- channels with different conduc-tance in human sperm membrane at least. (Asian J Androl 2001 Sep; 3: 185 - 191)
基金Grants supporting this project are from the National Key Research and Development Program of China(2021YFA1300601)National Natural Science Foundation of China grants(82273048 and 82341015).
文摘In a recent study published in Nature,Huang,Wong and their colleagues identified G2E3 as the major histone H3 lysine 14 mono-ubiquitin(H3K14ub)ligase in mammalian cells.1 By coupling G2E3-dependent H3K14 ubiquitination to the recruitment of SUV39H at pericentromeric chromatin,the authors define a conserved pathway that guides the reassembly of H3K9me3 domains after mitosis and secures the proper compartmentalization of chromatin.
