Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune res...Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune responses. The gC II complex consists of two glycoproteins, gM and gN. In the current study, DNA vaccines expressing the murine cytomegalovirus (MCMV) homologs of the gM and gN proteins were evaluated for protection against lethal MCMV infection in a mouse model. Humoral and cellular immune responses, spleen viral titers, and mice survival and body-weight changes were examined. The results showed that immunization with gM or gN DNA vaccine alone was not able to offer good protection, whereas co-immunization with both gM and gN induced an effective neutralizing antibody response and cellular immune response, and provided mice with complete protection against a lethal MCMV challenge. This study provides the first in vivo evidence that the gC II (gM-gN) complex may be able to serve as a protective subunit antigen for future HCMV vaccine development.展开更多
为更全面提取转子故障特征,将全矢谱和复局部均值分解(Complex local mean decomposition,CLMD)相结合,提出二元的全矢包络技术——CLMD全矢包络技术。采用正交采样技术获取转子同一截面上互相垂直方向上的振动信号,并将其组成一个复数...为更全面提取转子故障特征,将全矢谱和复局部均值分解(Complex local mean decomposition,CLMD)相结合,提出二元的全矢包络技术——CLMD全矢包络技术。采用正交采样技术获取转子同一截面上互相垂直方向上的振动信号,并将其组成一个复数信号;运用CLMD将复数信号按能量从高到低的顺序依次分离出系列复乘积函数(Complex product function,CPF),并解调出CPF的复包络;由于故障特征主要在能量较高的CPF分量中,通过全矢谱技术融合前几阶CPF分量的包络信号,得到相应的全矢包络谱。仿真的调幅-调频信号分析结果表面,较之Hilbert解调,CLMD全矢包络技术可提取隐含的调频信息,而且不存在虚假的低频谱线。转子试验台模拟的基座松动信号、碰摩信号分析结果表明,较之单源信息的包络谱,CLMD全矢包络技术提取的谱线特征更清晰、全面,而且根据全矢包络谱可有效区分基座松动引起的碰摩和单一碰摩故障。展开更多
基金supported by the Innovation Platform Open Fund of Hunan Provincial Education Department (11K010)a research fund from Hunan Provincial Science and Technology Development (2008TP4033-2)
文摘Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune responses. The gC II complex consists of two glycoproteins, gM and gN. In the current study, DNA vaccines expressing the murine cytomegalovirus (MCMV) homologs of the gM and gN proteins were evaluated for protection against lethal MCMV infection in a mouse model. Humoral and cellular immune responses, spleen viral titers, and mice survival and body-weight changes were examined. The results showed that immunization with gM or gN DNA vaccine alone was not able to offer good protection, whereas co-immunization with both gM and gN induced an effective neutralizing antibody response and cellular immune response, and provided mice with complete protection against a lethal MCMV challenge. This study provides the first in vivo evidence that the gC II (gM-gN) complex may be able to serve as a protective subunit antigen for future HCMV vaccine development.
文摘为更全面提取转子故障特征,将全矢谱和复局部均值分解(Complex local mean decomposition,CLMD)相结合,提出二元的全矢包络技术——CLMD全矢包络技术。采用正交采样技术获取转子同一截面上互相垂直方向上的振动信号,并将其组成一个复数信号;运用CLMD将复数信号按能量从高到低的顺序依次分离出系列复乘积函数(Complex product function,CPF),并解调出CPF的复包络;由于故障特征主要在能量较高的CPF分量中,通过全矢谱技术融合前几阶CPF分量的包络信号,得到相应的全矢包络谱。仿真的调幅-调频信号分析结果表面,较之Hilbert解调,CLMD全矢包络技术可提取隐含的调频信息,而且不存在虚假的低频谱线。转子试验台模拟的基座松动信号、碰摩信号分析结果表明,较之单源信息的包络谱,CLMD全矢包络技术提取的谱线特征更清晰、全面,而且根据全矢包络谱可有效区分基座松动引起的碰摩和单一碰摩故障。
