An image fusion method combining complex contourlet transform(CCT) with nonnegative matrix factorization(NMF) is proposed in this paper.After two images are decomposed by CCT,NMF is applied to their highand low-freque...An image fusion method combining complex contourlet transform(CCT) with nonnegative matrix factorization(NMF) is proposed in this paper.After two images are decomposed by CCT,NMF is applied to their highand low-frequency components,respectively,and finally an image is synthesized.Subjective-visual-quality of the image fusion result is compared with those of the image fusion methods based on NMF and the combination of wavelet /contourlet /nonsubsampled contourlet with NMF.The experimental results are evaluated quantitatively,and the running time is also contrasted.It is shown that the proposed image fusion method can gain larger information entropy,standard deviation and mean gradient,which means that it can better integrate featured information from all source images,avoid background noise and promote space clearness in the fusion image effectively.展开更多
Finding crucial vertices is a key problem for improving the reliability and ensuring the effective operation of networks,solved by approaches based on multiple attribute decision that suffer from ignoring the correlat...Finding crucial vertices is a key problem for improving the reliability and ensuring the effective operation of networks,solved by approaches based on multiple attribute decision that suffer from ignoring the correlation among each attribute or the heterogeneity between attribute and structure. To overcome these problems, a novel vertex centrality approach, called VCJG, is proposed based on joint nonnegative matrix factorization and graph embedding. The potential attributes with linearly independent and the structure information are captured automatically in light of nonnegative matrix factorization for factorizing the weighted adjacent matrix and the structure matrix, which is generated by graph embedding. And the smoothness strategy is applied to eliminate the heterogeneity between attributes and structure by joint nonnegative matrix factorization. Then VCJG integrates the above steps to formulate an overall objective function, and obtain the ultimately potential attributes fused the structure information of network through optimizing the objective function. Finally, the attributes are combined with neighborhood rules to evaluate vertex's importance. Through comparative analyses with experiments on nine real-world networks, we demonstrate that the proposed approach outperforms nine state-of-the-art algorithms for identification of vital vertices with respect to correlation, monotonicity and accuracy of top-10 vertices ranking.展开更多
Charge-transfer complexes(CTCs)have emerged as promising n-type organic thermoelectric(TE)materials due to their inherent high electrical conductivity and tunable transport polarities.In this study,we performed a comp...Charge-transfer complexes(CTCs)have emerged as promising n-type organic thermoelectric(TE)materials due to their inherent high electrical conductivity and tunable transport polarities.In this study,we performed a comprehensive first-principles investigation on the TE properties of nine CTCs comprised of 2,7-dialkyl[1]benzothieno[3,2-b][1]benzothiophenes(CnBTBT,n=4,8,12)as donors and fluorinated derivatives of tetracyanoquinodimethane(F_(m)TCNQ,m=0,2,4)as acceptors,aiming to identify high-performance n-type organic TE materials and elucidate the underlying structure-property relationships.Our calculation results,based on the Boltzmann transport equation and deformation potential theory,reveal that the length of the alkyl side chains and the number of fluorine substitutions significantly impact their electronic structures and TE properties.Notably,the CnBTBT-F_(m)TCNQ CTCs with shorter alkyl chains and more fluorine substitution demonstrate superior n-type characteristics,particularly C4BTBT-F4TCNQ,which achieves an excellent power factor of 671µW cm^(-1) K^(-2) at an optimal charge carrier concentration.Our findings not only clarify the critical role of molecular engineering in CTC-based TE materials but also provide valuable guidance for developing high-efficiency organic TE materials with versatile practical applications.展开更多
Objectives:The eukaryotic initiation factor 4F(eIF4F)translation initiation complex inhibitors(eIF4Fi)were recently found to hyperactivate extracellular signal-regulated kinases 1/2(ERK1/2)signals,which contribute to ...Objectives:The eukaryotic initiation factor 4F(eIF4F)translation initiation complex inhibitors(eIF4Fi)were recently found to hyperactivate extracellular signal-regulated kinases 1/2(ERK1/2)signals,which contribute to acquired resistance to BRAF(B-Raf proto-oncogene,serine/threonine kinase)inhibitors in melanoma.This present study aims to elucidate how to overcome the resistance of the eIF4Fi in BRAFV600E mutant melanoma cells and explore the underlying mechanisms.Methods:Melanoma A375(vemurafenib[VEM]-sensitive)and A375R(VEM-resistant)cells were exposed to eIF4Fi RocA at varying doses and durations in vitro.We investigated the impact of RocA on the activity of ERK1/2,AKT serine/threonine kinase 1(AKT1),eIF4E,and enhancer of zeste homolog 2(EZH2).We then examined the impact of RocA on pro-apoptotic BH3-only proteins and proliferative proteins.We subsequently determined the effect of combined eIF4Fi,AKT1 inhibitor,EZH2 inhibitor or VEM on tumor growth in vitro and in vivo.Results:RocA inhibited proliferation and induced apoptosis in A375 cells,but inhibited proliferation in A375R cells.RocA rapidly reactivated ERK1/2 at 3 h and returned to baseline levels at 48 h.However,eIF4E and AKT1 activation began at 12 h and peaked at 48 h.ERK1/2 positively regulated EZH2 and EZH2-dependent expression of c-Fos and EGR1,while AKT1 negatively regulated c-Myc,c-Jun,and BMF,but positively regulated eIF4E.RocA downregulated ERK1/2(or EZH2,AKT1,and eIF4E)independent bcl-2 and Mcl-1 expression.AKT1i enhanced RocA-induced cell apoptosis,while EZH2i reduced RocA-induced cell proliferation.Combined CR-1-31-B,EZH2i,and AKT1i effectively overcame resistance to RocA and VEM resistance both in vitro and in vivo.Conclusion:The eIF4F complex inhibitor reactivates ERK1/2-EZH2 and AKT1 signaling pathways,resulting in resistance to both eIF4Fi and VEM.Combined administration of an eIF4Fi with EZH2 and AKT1 inhibitors effectively enhances sensitivity to both eIF4F complex and BRAF inhibitors.展开更多
One of the most important problems in complex networks is to identify the influential vertices for understanding and controlling of information diffusion and disease spreading.Most of the current centrality algorithms...One of the most important problems in complex networks is to identify the influential vertices for understanding and controlling of information diffusion and disease spreading.Most of the current centrality algorithms focus on single feature or manually extract the attributes,which occasionally results in the failure to fully capture the vertex’s importance.A new vertex centrality approach based on symmetric nonnegative matrix factorization(SNMF),called VCSNMF,is proposed in this paper.For highlight the characteristics of a network,the adjacency matrix and the degree matrix are fused to represent original data of the network via a weighted linear combination.First,SNMF automatically extracts the latent characteristics of vertices by factorizing the established original data matrix.Then we prove that each vertex’s composite feature which is constructed with one-dimensional factor matrix can be approximated as the term of eigenvector associated with the spectral radius of the network,otherwise obtained by the factor matrix on the hyperspace.Finally,VCSNMF integrates the composite feature and the topological structure to evaluate the performance of vertices.To verify the effectiveness of the VCSNMF criterion,eight existing centrality approaches are used as comparison measures to rank influential vertices in ten real-world networks.The experimental results assert the superiority of the method.展开更多
Based on the estimating rule of the normal vector angles between two adjacent terrain units, we use the concept of terrain complexity factor to quantify the terrain complexity of DEM, and then the formula of terrain c...Based on the estimating rule of the normal vector angles between two adjacent terrain units, we use the concept of terrain complexity factor to quantify the terrain complexity of DEM, and then the formula of terrain complexity factor in Raster DEM and TIN DEM is deduced theoretically. In order to make clear how the terrain complexity factor ECF and the average elevation h affect the accuracy of DEM terrain representation RMSEEt, the formula of Gauss synthetical surface is applied to simulate several real terrain surfaces, each of which has different terrain complexity. Through the statistical analysis of linear regression in simula- tion data, the linear equation between accuracy of DEM terrain representation RMSEEt, terrain complexity factor ECF and the average elevation h is achieved. A new method is provided to estimate the accuracy of DEM terrain representation RMSEEt with a certain terrain complexity and it gives convincing theoretical evidence for DEM production and the corresponding error research in the future.展开更多
The equivalent circuit with complex physical constants for a piezoelectric ceramic in thickness mode is established. In the equivalent circuit, electric components （equivalent circuit parameters） are connected to re...The equivalent circuit with complex physical constants for a piezoelectric ceramic in thickness mode is established. In the equivalent circuit, electric components （equivalent circuit parameters） are connected to real and imaginary parts of complex physical coefficients of piezoelectric materials. Based on definitions of dissipation factors, three of them （dielectric, elastic and piezoelectric dissipation factors） are represented by equivalent circuit parameters. Since the equivalent circuit parameters are detectable, the dissipation factors can be easily obtained. In the experiments, the temperature and the stress responses of the three dissipation factors are measured.展开更多
The compact implicit integration factor （cIIF） method is an efficient time discretization scheme for stiff nonlinear diffusion equations in two and three spatial dimensions. In the current work, we apply the cIIF me...The compact implicit integration factor （cIIF） method is an efficient time discretization scheme for stiff nonlinear diffusion equations in two and three spatial dimensions. In the current work, we apply the cIIF method to some complex-valued nonlinear evolutionary equations such as the nonlinear SchrSdinger （NLS） equation and the complex Ginzburg-Landau （GL） equation. Detailed algorithm formulation and practical implementation of cIIF method are performed. The numerical results indicate that this method is very accurate and efficient.展开更多
The defining neuropathological feature of Parkinson＇s disease （PD） is the loss of nigrostriatal dopaminergic （DA） projections. This results in striatal dopamine levels and a biochemical reduction of movement diso...The defining neuropathological feature of Parkinson＇s disease （PD） is the loss of nigrostriatal dopaminergic （DA） projections. This results in striatal dopamine levels and a biochemical reduction of movement disorders, such as a tremor at rest, rigidity of the limbs, bradykinesia, and postural instability （Kim et al., 2011; Kim et al., 2012; Burke and O＇Malley, 2013; Leem et al., 2014; Namet al., 2014）.展开更多
Taking cruciform welded joints under different corrosion degrees, together with 45° inclined angle and full penetration load-carrying fillet, as the research object, the stress intensity factor of a quarter-circu...Taking cruciform welded joints under different corrosion degrees, together with 45° inclined angle and full penetration load-carrying fillet, as the research object, the stress intensity factor of a quarter-circular comer crack of welded joints is calculated based on FRANC3D with ABAQUS. Effects of different corrosion pit sizes, crack aspect ratios, and crack depths on stress intensity factor are analyzed. The results show that pit depth plays a major role in stress intensity factor, while the effect of pit radius is relatively small. The cracking modes of the surface and the deepest point are mode I, and mixed modes I and II, respectively. Effects of pit depths, crack aspect ratios, and crack depths on the stress intensity factor at the surface point are greater than at the deepest point.展开更多
Aim: To determine the frequency and the risk factors of Complex Regional Pain syndrome (CRPS) in Cotonou (Benin). Patients and Méthod: This has been a transversal study carried out over 22 years on files of CRPS ...Aim: To determine the frequency and the risk factors of Complex Regional Pain syndrome (CRPS) in Cotonou (Benin). Patients and Méthod: This has been a transversal study carried out over 22 years on files of CRPS infected patients and submitted to rheumatologic consultation in the National Hospital University of Cotonou. Results: 73 out of 17,342 patients examined (0.42%) were suffering from CRPS. Those 73 patients (40 women, 54.8% and 33 men, 45.2%) were in average 54.66 years old and enjoyed an average duration of evolution of 5.79 months. The trauma (41 cases, 56.1%), the stroke (19 cases, 25%), the diabetes (8 cases, 11.3), were the main risk factors that were observed. CRPS was preferably located at the shoulder-hand (34.2%), shoulder (28.8%), wrist-hand (16.4%) and knee (11%). Inflammatory pain was observed in 55 cases. The treatment was dominated by griseofulvina (41 cases, 56.1%), antiinflammatory drugs (38 cases, 52%), analgesic (20 cases, 27, 3%), joint injection by betamethasone (17 cases, 27%). Conclusion: CRPS is not rare in our country. The first risk factor remains the trauma in rheumatologic consultation in Cotonou.展开更多
To increase the variety and security of communication, we present the definitions of modified projective synchronization with complex scaling factors （CMPS） of real chaotic systems and complex chaotic systems, where...To increase the variety and security of communication, we present the definitions of modified projective synchronization with complex scaling factors （CMPS） of real chaotic systems and complex chaotic systems, where complex scaling factors establish a link between real chaos and complex chaos. Considering all situations of unknown parameters and pseudo-gradient condition, we design adaptive CMPS schemes based on the speed-gradient method for the real drive chaotic system and complex response chaotic system and for the complex drive chaotic system and the real response chaotic system, respectively. The convergence factors and dynamical control strength are added to regulate the convergence speed and increase robustness. Numerical simulations verify the feasibility and effectiveness of the presented schemes.展开更多
Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteoro...Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteorological factors during 2012-2016. Our findings revealed that 1) mean PM2.5 levels gradually decreased from 30.70 μg/m3 in 2013 to 25.36 μg/m3 in 2016;2) wind speed is the main determinant of air quality—air quality significantly improved when it was faster than 4 m/sec;and 3) wind direction is another determinant of air quality—when the wind direction was southerly, air quality improved. Elevated PM2.5 levels were defined as those hourly levels higher than the third quartile (36 μg/m3). The significantly negative predictive factors for elevated PM2.5 levels were the summer or autumn seasons, rainfall, increased wind speed, and wind direction from 150° to 230° from the north. The significantly positive predictive factors for elevated PM2.5 levels were working hours from 6 a.m. to 2 p.m., a temperature between 11°C and 25°C, relative humidity between 40% and 68%, and wind direction (e.g., northerly wind, northeasterly wind, and easterly wind). The predictive formula is attached in the Appendix. Therefore, people should protect themselves on these high-risk days.展开更多
In this paper,the influence of crystal-field on the Luminescence properties of Eu^(2+) in complex oxides are studied theoretically by using purely electrostatic model,the dependence of the 4f^65d levels on Eu-O bond d...In this paper,the influence of crystal-field on the Luminescence properties of Eu^(2+) in complex oxides are studied theoretically by using purely electrostatic model,the dependence of the 4f^65d levels on Eu-O bond distance is given.Quantum chemistry calculation shows that the splitting extent of 4f^65d energy band in cubic or in octahedral fields will be inversely proportional to R^5,where R is the distance of Eu^(2+) to oxygen ligand.The value of R affects slightly the location of the centre of 4f^65d energy band.According to the exper- imental spectrum data,we have discussed the influence of the host chemical composition,the replaced sites of Eu^(2+) and degree of covalency of Eu-O bond on luminescence properties of Eu^(2+).Some regularity of fluorescence spectrum was observed. In alkali-alkaline earth-phosphates,the splitting extent of 4f^65d band (△E) becomes smaller as the Eu-O bond distance (R) increases.In Na_(3-x)(PO_4)_(1-x)(SO_4)_x and Na_(2-x)CaSi_(1-x)P_xO_4 hosts,d-d emission peak of Eu^(2+) will shift to shorter wavelength with the increase of x's value. The crystal structure data show that Eu^(2+) in K_2Mg_2(SO_4)_3 is affected more strongly by crystal-field and covalancy than in KMgF_3,so K_2Mg_2(SO_4)_3:Eu^(2+) emits blue light (E_(em)~m=400nm) and KMgF_3:Eu^(2+) produces ultraviolet fluorescence.展开更多
Numerous studies have examined the impact ofwater quality degradation on bacterial community structure,yet insights into its effects on the bacterial ecological networks remain scarce.In this study,we investigated the...Numerous studies have examined the impact ofwater quality degradation on bacterial community structure,yet insights into its effects on the bacterial ecological networks remain scarce.In this study,we investigated the diversity,composition,assembly patterns,ecological networks,and environmental determinants of bacterial communities across 20 ponds to understand the impact of water quality degradation.Our findings revealed that water quality degradation significantly reduces the α-diversity of bacterial communities in water samples,while sediment samples remain unaffected.Additionally,water quality deterioration increases the complexity of bacterial networks in water samples but reduces it in sediment samples.These shifts in bacterial communities were primarily governed by deterministic processes,with heterogeneous selection being particularly influential.Through redundancy analysis(RDA),multiple regression on matrices(MRM),and Mantel tests,we identified dissolved oxygen(DO),ammonium nitrogen(NH_(4)^(+)-N),and C/N ratio as key factors affecting the composition and network complexity of bacterial communities in both water and sediment.Overall,this study contributes a novel perspective on the effect ofwater quality deterioration on microbial ecosystems and provides valuable insights for improving ecological evaluations and biomonitoring practices related to water quality management.展开更多
Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 y...Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently regeneration.However,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains elusive.In this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.展开更多
BACKGROUND Data regarding complex Crohn’s perianal fistulas(CPF)epidemiology are limited,and optimal treatment strategies are elusive.An improved understanding of how CPF treatment options are used in the real-world ...BACKGROUND Data regarding complex Crohn’s perianal fistulas(CPF)epidemiology are limited,and optimal treatment strategies are elusive.An improved understanding of how CPF treatment options are used in the real-world setting and factors associated with CPF development,treatment failure,and reasons for undergoing multiple surgeries may help to inform optimal patient management strategies,reduce treatment burden,and improve outcomes in patients with CPF.AIM To describe the epidemiology,treatments,outcomes,and associated risk/protective factors for complex CPF.METHODS Electronic databases(MEDLINE,EMBASE,EBM Reviews,EconLit)were searched.Two reviewers independently used populations,interventions,comparators,outcomes,study designs,and time criteria to identify relevant studies.Observational studies published in English from January 1,2015 to February 17,2022 with>50 patients were included,even if complex CPF was not defined.Items of interest included complex CPF definitions,epidemiology,treatment patterns,morbidity,mortality,and risk factors associated with complex CPF development,treatment failure,and undergoing multiple surgeries.Data were reported using descriptive statistics.RESULTS Overall,140 studies were included.Complex CPF definitions were heterogeneous and rarely reported(24 studies).Hence,data mostly related to CPF in general.CPF prevalence was variable(range:1.5%-81.0%).Incidence was wide-ranging and mostly reported cumulatively at 1-year post-Crohn’s disease diagnosis(range:3.5%-50.1%).Overall healing and failure rates after treatment were in the range of 10.5%-80.2%and 3.6%-83.0%,respectively.Abscesses were the most frequently reported morbidity(n=18).No CPF-related deaths were reported.No consistent risk or protective factors were identified.CONCLUSION Epidemiology,treatment patterns,and risk factors for CPF vary,likely due to inconsistent CPF and clinical outcome definitions.Standardization would facilitate comparability,which may inform optimal complex CPF treatment strategies.展开更多
In this editorial,we comment on the article by Zhang et al recently published in the World Journal of Gastroenterology.The manuscript elucidates significant novel mechanisms underlying hepatocellular carcinoma(HCC)pro...In this editorial,we comment on the article by Zhang et al recently published in the World Journal of Gastroenterology.The manuscript elucidates significant novel mechanisms underlying hepatocellular carcinoma(HCC)progression.HCC is currently considered one of the major causes of global cancer-associated deaths,underscoring the critical need for novel therapeutic targets.Growing evidence underlines the role of the lipid raft protein flotillin-1(FLOT1)in cancer,whose dysregulation drives tumor cell growth and survival.However,the regulatory role of FLOT1 on Golgi apparatus function in HCC is unknown.In this study,Zhang et al elucidated a pivotal mechanism by which FLOT1 promotes HCC progression through activation of transcription factor E3-mediated Golgi stress response.The study reveals that FLOT1 inhibits the mechanistic target of rapamycin complexes 1 and 2 by ubiquitination,facilitating transcription factor E3 dephosphorylation,nuclear translocation,and subsequent upregulation of Golgi stress-associated genes,thereby leading to enhanced HCC cell growth and invasive capacity.These findings obtained in vitro/in vivo highlight the interplay between FLOT1 and Golgi homeostasis in HCC.Targeting FLOT1 may offer a new strategy for the treatment of HCC.展开更多
基金Supported by National Natural Science Foundation of China (No. 60872065)
文摘An image fusion method combining complex contourlet transform(CCT) with nonnegative matrix factorization(NMF) is proposed in this paper.After two images are decomposed by CCT,NMF is applied to their highand low-frequency components,respectively,and finally an image is synthesized.Subjective-visual-quality of the image fusion result is compared with those of the image fusion methods based on NMF and the combination of wavelet /contourlet /nonsubsampled contourlet with NMF.The experimental results are evaluated quantitatively,and the running time is also contrasted.It is shown that the proposed image fusion method can gain larger information entropy,standard deviation and mean gradient,which means that it can better integrate featured information from all source images,avoid background noise and promote space clearness in the fusion image effectively.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.62162040 and 11861045)。
文摘Finding crucial vertices is a key problem for improving the reliability and ensuring the effective operation of networks,solved by approaches based on multiple attribute decision that suffer from ignoring the correlation among each attribute or the heterogeneity between attribute and structure. To overcome these problems, a novel vertex centrality approach, called VCJG, is proposed based on joint nonnegative matrix factorization and graph embedding. The potential attributes with linearly independent and the structure information are captured automatically in light of nonnegative matrix factorization for factorizing the weighted adjacent matrix and the structure matrix, which is generated by graph embedding. And the smoothness strategy is applied to eliminate the heterogeneity between attributes and structure by joint nonnegative matrix factorization. Then VCJG integrates the above steps to formulate an overall objective function, and obtain the ultimately potential attributes fused the structure information of network through optimizing the objective function. Finally, the attributes are combined with neighborhood rules to evaluate vertex's importance. Through comparative analyses with experiments on nine real-world networks, we demonstrate that the proposed approach outperforms nine state-of-the-art algorithms for identification of vital vertices with respect to correlation, monotonicity and accuracy of top-10 vertices ranking.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Science(No.XDB0520000)the National Natural Science Foundation of China(Nos.52273170 and 52394271)the National Key R&D Program of China(No.2022YFA1203200).
文摘Charge-transfer complexes(CTCs)have emerged as promising n-type organic thermoelectric(TE)materials due to their inherent high electrical conductivity and tunable transport polarities.In this study,we performed a comprehensive first-principles investigation on the TE properties of nine CTCs comprised of 2,7-dialkyl[1]benzothieno[3,2-b][1]benzothiophenes(CnBTBT,n=4,8,12)as donors and fluorinated derivatives of tetracyanoquinodimethane(F_(m)TCNQ,m=0,2,4)as acceptors,aiming to identify high-performance n-type organic TE materials and elucidate the underlying structure-property relationships.Our calculation results,based on the Boltzmann transport equation and deformation potential theory,reveal that the length of the alkyl side chains and the number of fluorine substitutions significantly impact their electronic structures and TE properties.Notably,the CnBTBT-F_(m)TCNQ CTCs with shorter alkyl chains and more fluorine substitution demonstrate superior n-type characteristics,particularly C4BTBT-F4TCNQ,which achieves an excellent power factor of 671µW cm^(-1) K^(-2) at an optimal charge carrier concentration.Our findings not only clarify the critical role of molecular engineering in CTC-based TE materials but also provide valuable guidance for developing high-efficiency organic TE materials with versatile practical applications.
文摘Objectives:The eukaryotic initiation factor 4F(eIF4F)translation initiation complex inhibitors(eIF4Fi)were recently found to hyperactivate extracellular signal-regulated kinases 1/2(ERK1/2)signals,which contribute to acquired resistance to BRAF(B-Raf proto-oncogene,serine/threonine kinase)inhibitors in melanoma.This present study aims to elucidate how to overcome the resistance of the eIF4Fi in BRAFV600E mutant melanoma cells and explore the underlying mechanisms.Methods:Melanoma A375(vemurafenib[VEM]-sensitive)and A375R(VEM-resistant)cells were exposed to eIF4Fi RocA at varying doses and durations in vitro.We investigated the impact of RocA on the activity of ERK1/2,AKT serine/threonine kinase 1(AKT1),eIF4E,and enhancer of zeste homolog 2(EZH2).We then examined the impact of RocA on pro-apoptotic BH3-only proteins and proliferative proteins.We subsequently determined the effect of combined eIF4Fi,AKT1 inhibitor,EZH2 inhibitor or VEM on tumor growth in vitro and in vivo.Results:RocA inhibited proliferation and induced apoptosis in A375 cells,but inhibited proliferation in A375R cells.RocA rapidly reactivated ERK1/2 at 3 h and returned to baseline levels at 48 h.However,eIF4E and AKT1 activation began at 12 h and peaked at 48 h.ERK1/2 positively regulated EZH2 and EZH2-dependent expression of c-Fos and EGR1,while AKT1 negatively regulated c-Myc,c-Jun,and BMF,but positively regulated eIF4E.RocA downregulated ERK1/2(or EZH2,AKT1,and eIF4E)independent bcl-2 and Mcl-1 expression.AKT1i enhanced RocA-induced cell apoptosis,while EZH2i reduced RocA-induced cell proliferation.Combined CR-1-31-B,EZH2i,and AKT1i effectively overcame resistance to RocA and VEM resistance both in vitro and in vivo.Conclusion:The eIF4F complex inhibitor reactivates ERK1/2-EZH2 and AKT1 signaling pathways,resulting in resistance to both eIF4Fi and VEM.Combined administration of an eIF4Fi with EZH2 and AKT1 inhibitors effectively enhances sensitivity to both eIF4F complex and BRAF inhibitors.
基金the National Natural Science Foundation of China(Nos.11361033 and 11861045)。
文摘One of the most important problems in complex networks is to identify the influential vertices for understanding and controlling of information diffusion and disease spreading.Most of the current centrality algorithms focus on single feature or manually extract the attributes,which occasionally results in the failure to fully capture the vertex’s importance.A new vertex centrality approach based on symmetric nonnegative matrix factorization(SNMF),called VCSNMF,is proposed in this paper.For highlight the characteristics of a network,the adjacency matrix and the degree matrix are fused to represent original data of the network via a weighted linear combination.First,SNMF automatically extracts the latent characteristics of vertices by factorizing the established original data matrix.Then we prove that each vertex’s composite feature which is constructed with one-dimensional factor matrix can be approximated as the term of eigenvector associated with the spectral radius of the network,otherwise obtained by the factor matrix on the hyperspace.Finally,VCSNMF integrates the composite feature and the topological structure to evaluate the performance of vertices.To verify the effectiveness of the VCSNMF criterion,eight existing centrality approaches are used as comparison measures to rank influential vertices in ten real-world networks.The experimental results assert the superiority of the method.
基金Supported by Innovation Program of Shanghai Municipal Education Commission (No.10ZZ25)the Key Laboratory of Geo-informatics of State Bureau of Surveying and Mapping (No.200914)
文摘Based on the estimating rule of the normal vector angles between two adjacent terrain units, we use the concept of terrain complexity factor to quantify the terrain complexity of DEM, and then the formula of terrain complexity factor in Raster DEM and TIN DEM is deduced theoretically. In order to make clear how the terrain complexity factor ECF and the average elevation h affect the accuracy of DEM terrain representation RMSEEt, the formula of Gauss synthetical surface is applied to simulate several real terrain surfaces, each of which has different terrain complexity. Through the statistical analysis of linear regression in simula- tion data, the linear equation between accuracy of DEM terrain representation RMSEEt, terrain complexity factor ECF and the average elevation h is achieved. A new method is provided to estimate the accuracy of DEM terrain representation RMSEEt with a certain terrain complexity and it gives convincing theoretical evidence for DEM production and the corresponding error research in the future.
基金Project supported by the National Natural Science Foundation of China （Grant No 50278098）.
文摘The equivalent circuit with complex physical constants for a piezoelectric ceramic in thickness mode is established. In the equivalent circuit, electric components （equivalent circuit parameters） are connected to real and imaginary parts of complex physical coefficients of piezoelectric materials. Based on definitions of dissipation factors, three of them （dielectric, elastic and piezoelectric dissipation factors） are represented by equivalent circuit parameters. Since the equivalent circuit parameters are detectable, the dissipation factors can be easily obtained. In the experiments, the temperature and the stress responses of the three dissipation factors are measured.
文摘The compact implicit integration factor （cIIF） method is an efficient time discretization scheme for stiff nonlinear diffusion equations in two and three spatial dimensions. In the current work, we apply the cIIF method to some complex-valued nonlinear evolutionary equations such as the nonlinear SchrSdinger （NLS） equation and the complex Ginzburg-Landau （GL） equation. Detailed algorithm formulation and practical implementation of cIIF method are performed. The numerical results indicate that this method is very accurate and efficient.
文摘The defining neuropathological feature of Parkinson＇s disease （PD） is the loss of nigrostriatal dopaminergic （DA） projections. This results in striatal dopamine levels and a biochemical reduction of movement disorders, such as a tremor at rest, rigidity of the limbs, bradykinesia, and postural instability （Kim et al., 2011; Kim et al., 2012; Burke and O＇Malley, 2013; Leem et al., 2014; Namet al., 2014）.
基金supported by the National Natural Science Foundation of China(51378430 and 51378431)
文摘Taking cruciform welded joints under different corrosion degrees, together with 45° inclined angle and full penetration load-carrying fillet, as the research object, the stress intensity factor of a quarter-circular comer crack of welded joints is calculated based on FRANC3D with ABAQUS. Effects of different corrosion pit sizes, crack aspect ratios, and crack depths on stress intensity factor are analyzed. The results show that pit depth plays a major role in stress intensity factor, while the effect of pit radius is relatively small. The cracking modes of the surface and the deepest point are mode I, and mixed modes I and II, respectively. Effects of pit depths, crack aspect ratios, and crack depths on the stress intensity factor at the surface point are greater than at the deepest point.
文摘Aim: To determine the frequency and the risk factors of Complex Regional Pain syndrome (CRPS) in Cotonou (Benin). Patients and Méthod: This has been a transversal study carried out over 22 years on files of CRPS infected patients and submitted to rheumatologic consultation in the National Hospital University of Cotonou. Results: 73 out of 17,342 patients examined (0.42%) were suffering from CRPS. Those 73 patients (40 women, 54.8% and 33 men, 45.2%) were in average 54.66 years old and enjoyed an average duration of evolution of 5.79 months. The trauma (41 cases, 56.1%), the stroke (19 cases, 25%), the diabetes (8 cases, 11.3), were the main risk factors that were observed. CRPS was preferably located at the shoulder-hand (34.2%), shoulder (28.8%), wrist-hand (16.4%) and knee (11%). Inflammatory pain was observed in 55 cases. The treatment was dominated by griseofulvina (41 cases, 56.1%), antiinflammatory drugs (38 cases, 52%), analgesic (20 cases, 27, 3%), joint injection by betamethasone (17 cases, 27%). Conclusion: CRPS is not rare in our country. The first risk factor remains the trauma in rheumatologic consultation in Cotonou.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61273088,10971120,and 61001099)the Natural Science Foundation of Shandong Province,China(Grant No.ZR2010FM010)
文摘To increase the variety and security of communication, we present the definitions of modified projective synchronization with complex scaling factors （CMPS） of real chaotic systems and complex chaotic systems, where complex scaling factors establish a link between real chaos and complex chaos. Considering all situations of unknown parameters and pseudo-gradient condition, we design adaptive CMPS schemes based on the speed-gradient method for the real drive chaotic system and complex response chaotic system and for the complex drive chaotic system and the real response chaotic system, respectively. The convergence factors and dynamical control strength are added to regulate the convergence speed and increase robustness. Numerical simulations verify the feasibility and effectiveness of the presented schemes.
文摘Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteorological factors during 2012-2016. Our findings revealed that 1) mean PM2.5 levels gradually decreased from 30.70 μg/m3 in 2013 to 25.36 μg/m3 in 2016;2) wind speed is the main determinant of air quality—air quality significantly improved when it was faster than 4 m/sec;and 3) wind direction is another determinant of air quality—when the wind direction was southerly, air quality improved. Elevated PM2.5 levels were defined as those hourly levels higher than the third quartile (36 μg/m3). The significantly negative predictive factors for elevated PM2.5 levels were the summer or autumn seasons, rainfall, increased wind speed, and wind direction from 150° to 230° from the north. The significantly positive predictive factors for elevated PM2.5 levels were working hours from 6 a.m. to 2 p.m., a temperature between 11°C and 25°C, relative humidity between 40% and 68%, and wind direction (e.g., northerly wind, northeasterly wind, and easterly wind). The predictive formula is attached in the Appendix. Therefore, people should protect themselves on these high-risk days.
文摘In this paper,the influence of crystal-field on the Luminescence properties of Eu^(2+) in complex oxides are studied theoretically by using purely electrostatic model,the dependence of the 4f^65d levels on Eu-O bond distance is given.Quantum chemistry calculation shows that the splitting extent of 4f^65d energy band in cubic or in octahedral fields will be inversely proportional to R^5,where R is the distance of Eu^(2+) to oxygen ligand.The value of R affects slightly the location of the centre of 4f^65d energy band.According to the exper- imental spectrum data,we have discussed the influence of the host chemical composition,the replaced sites of Eu^(2+) and degree of covalency of Eu-O bond on luminescence properties of Eu^(2+).Some regularity of fluorescence spectrum was observed. In alkali-alkaline earth-phosphates,the splitting extent of 4f^65d band (△E) becomes smaller as the Eu-O bond distance (R) increases.In Na_(3-x)(PO_4)_(1-x)(SO_4)_x and Na_(2-x)CaSi_(1-x)P_xO_4 hosts,d-d emission peak of Eu^(2+) will shift to shorter wavelength with the increase of x's value. The crystal structure data show that Eu^(2+) in K_2Mg_2(SO_4)_3 is affected more strongly by crystal-field and covalancy than in KMgF_3,so K_2Mg_2(SO_4)_3:Eu^(2+) emits blue light (E_(em)~m=400nm) and KMgF_3:Eu^(2+) produces ultraviolet fluorescence.
基金supported by Zhejiang Provincial Natural Science Foundation of China(No.LTGS24D010004)the National Natural Science Foundation of China grant(No.42307064)+2 种基金the National Students’platform for innovation and entrepreneurship training program(No.202410346054)Hangzhou“Young science and technology talent cultivation”project(No.4305F45623004)the Fundamental Research Funds for Climbing Project from Hangzhou Normal University(No.KYQD-2023-217).
文摘Numerous studies have examined the impact ofwater quality degradation on bacterial community structure,yet insights into its effects on the bacterial ecological networks remain scarce.In this study,we investigated the diversity,composition,assembly patterns,ecological networks,and environmental determinants of bacterial communities across 20 ponds to understand the impact of water quality degradation.Our findings revealed that water quality degradation significantly reduces the α-diversity of bacterial communities in water samples,while sediment samples remain unaffected.Additionally,water quality deterioration increases the complexity of bacterial networks in water samples but reduces it in sediment samples.These shifts in bacterial communities were primarily governed by deterministic processes,with heterogeneous selection being particularly influential.Through redundancy analysis(RDA),multiple regression on matrices(MRM),and Mantel tests,we identified dissolved oxygen(DO),ammonium nitrogen(NH_(4)^(+)-N),and C/N ratio as key factors affecting the composition and network complexity of bacterial communities in both water and sediment.Overall,this study contributes a novel perspective on the effect ofwater quality deterioration on microbial ecosystems and provides valuable insights for improving ecological evaluations and biomonitoring practices related to water quality management.
基金supported by National Health and Medical Research Council GNT1105374,GNT1137645,GNT2000766 and veski Innovation Fellowship(VIF23)to RP.
文摘Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently regeneration.However,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains elusive.In this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.
基金Supported by Takeda Pharmaceuticals USA,Inc.Medical writing support was provided by Luke Humphreys,PhD,of Oxford PharmaGenesis,Oxford,UK and was funded by Takeda Development Center Americas,Inc.
文摘BACKGROUND Data regarding complex Crohn’s perianal fistulas(CPF)epidemiology are limited,and optimal treatment strategies are elusive.An improved understanding of how CPF treatment options are used in the real-world setting and factors associated with CPF development,treatment failure,and reasons for undergoing multiple surgeries may help to inform optimal patient management strategies,reduce treatment burden,and improve outcomes in patients with CPF.AIM To describe the epidemiology,treatments,outcomes,and associated risk/protective factors for complex CPF.METHODS Electronic databases(MEDLINE,EMBASE,EBM Reviews,EconLit)were searched.Two reviewers independently used populations,interventions,comparators,outcomes,study designs,and time criteria to identify relevant studies.Observational studies published in English from January 1,2015 to February 17,2022 with>50 patients were included,even if complex CPF was not defined.Items of interest included complex CPF definitions,epidemiology,treatment patterns,morbidity,mortality,and risk factors associated with complex CPF development,treatment failure,and undergoing multiple surgeries.Data were reported using descriptive statistics.RESULTS Overall,140 studies were included.Complex CPF definitions were heterogeneous and rarely reported(24 studies).Hence,data mostly related to CPF in general.CPF prevalence was variable(range:1.5%-81.0%).Incidence was wide-ranging and mostly reported cumulatively at 1-year post-Crohn’s disease diagnosis(range:3.5%-50.1%).Overall healing and failure rates after treatment were in the range of 10.5%-80.2%and 3.6%-83.0%,respectively.Abscesses were the most frequently reported morbidity(n=18).No CPF-related deaths were reported.No consistent risk or protective factors were identified.CONCLUSION Epidemiology,treatment patterns,and risk factors for CPF vary,likely due to inconsistent CPF and clinical outcome definitions.Standardization would facilitate comparability,which may inform optimal complex CPF treatment strategies.
基金Supported by Italian Association for Cancer Research(AIRC),No.21956Italian Ministry of Health-5×1000 funds 2023.
文摘In this editorial,we comment on the article by Zhang et al recently published in the World Journal of Gastroenterology.The manuscript elucidates significant novel mechanisms underlying hepatocellular carcinoma(HCC)progression.HCC is currently considered one of the major causes of global cancer-associated deaths,underscoring the critical need for novel therapeutic targets.Growing evidence underlines the role of the lipid raft protein flotillin-1(FLOT1)in cancer,whose dysregulation drives tumor cell growth and survival.However,the regulatory role of FLOT1 on Golgi apparatus function in HCC is unknown.In this study,Zhang et al elucidated a pivotal mechanism by which FLOT1 promotes HCC progression through activation of transcription factor E3-mediated Golgi stress response.The study reveals that FLOT1 inhibits the mechanistic target of rapamycin complexes 1 and 2 by ubiquitination,facilitating transcription factor E3 dephosphorylation,nuclear translocation,and subsequent upregulation of Golgi stress-associated genes,thereby leading to enhanced HCC cell growth and invasive capacity.These findings obtained in vitro/in vivo highlight the interplay between FLOT1 and Golgi homeostasis in HCC.Targeting FLOT1 may offer a new strategy for the treatment of HCC.
