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An Empirical Analysis of the Effect of Text-Based Computer-Mediated Communication on Communicative Competence
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作者 王剑娜 《海外英语》 2016年第8期225-228,共4页
It has been suggested that text-based computer-mediated communication can help learners to use target language both in classrooms and in social contexts.It’s necessary to investigate the effect of text-based CMC on l... It has been suggested that text-based computer-mediated communication can help learners to use target language both in classrooms and in social contexts.It’s necessary to investigate the effect of text-based CMC on learners’communicative competence by conducting the method of systematic review.The findings implied that text-based CMC settings allowed learners to interact.The interaction provided learners with more opportunities to develop their communicative competence of target language. 展开更多
关键词 text-based computer-mediated communication(CMC) second language acquisition(SLA) COMMUNICATIVE compe
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The effects of intraspecific competition and light transmission within the canopy on wheat yield in a wide-precision planting pattern 被引量:7
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作者 LIU Xin WANG Wen-xin +2 位作者 LIN Xiang GU Shu-bo WANG Dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第6期1577-1585,共9页
The wide-precision planting pattern has become widely used in the North China Plain as a practice for increasing wheat yield.However,the effects of tilled ng developme nt and light tran smissi on within can opy on whe... The wide-precision planting pattern has become widely used in the North China Plain as a practice for increasing wheat yield.However,the effects of tilled ng developme nt and light tran smissi on within can opy on wheat yield un der differe nt sowi ng widths have not bee n clearly described.Therefore,a two-year experime nt was con ducted,in cludi ng four different seeding widths(6 cm,W6;8 cm,W8;10 cm,W10;12 cm,W12)and the traditional planting pattern with seeding width of 4 cm(W4).The results indicated mainly positive effects by the reduced intraspecific competition,specifically all three yield components of W6 and W8 were higher than those for W4.The configurations with more than 10-cm seeding width were mainly affected by the negative effect of a relative homogeneous canopy,leading to the weakened light transmission,leaf senescence,and reduced grain number per spike.Finally,the yields of W6 and W8 were significantly higher than that of W4,whereas the yield in W12 was lower(though not significantly)than W4.In wheat production,therefore,the appropriate seeding width of 6-8 cm is recommended for farmers,whereas the too wide seeding width,with more than 10 cm,should be avoided. 展开更多
关键词 LIGHT tra nsmissi on seedi ng width TILLERS NU mber WIN ter wheat yield COMP orients
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On Improving Chinese Students' Listening and Speaking Competence in English 被引量:2
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作者 徐淑颖 《海外英语》 2012年第7X期31-32,共2页
Improving listening and speaking competence,the two fundamental communicative skills,has become widely accepted as an important goal of English teaching in China.But due to the grammar-focused teaching method,students... Improving listening and speaking competence,the two fundamental communicative skills,has become widely accepted as an important goal of English teaching in China.But due to the grammar-focused teaching method,students still have great difficulties in listening and speaking.This essay intends to reveal the methods of using the lexical vantage to improve communicative abilities. 展开更多
关键词 applied LINGUISTICS LEXICAL VANTAGE LISTENING comp
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An Analysis on Export Competitiveness of Vegetables from China to ASEAN
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作者 Shiwei WANG 《Asian Agricultural Research》 2015年第9期9-11 16,16,共4页
The establishment of China-ASEAN Free Trade Area has promoted the friendly bilateral economic cooperation,and ASEAN has become one of the largest vegetable export markets for China. Using constant market share( CMS) m... The establishment of China-ASEAN Free Trade Area has promoted the friendly bilateral economic cooperation,and ASEAN has become one of the largest vegetable export markets for China. Using constant market share( CMS) model,this paper analyzes the export competitiveness of vegetables from China to ASEAN and the main influencing factors. The results show that competitiveness is the main factor in promoting the export growth of vegetables from China to ASEAN,and compared with demand effect and structure effect,competitiveness effect makes the greatest contribution to export growth. 展开更多
关键词 VEGETABLES CONSTANT market SHARE model EXPORT COMP
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Evonik Radiation Curing Competence Centre Asia / Pacific Opened in Shanghai
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《China Chemical Reporter》 2010年第2期29-29,共11页
With the attendance of over 100 vips from Evonik andother companies,the ribbon of"Evonik Specialties RadiationCuring Competence Center Asia / Pacific" was cut in its openingceremony recently held in Shangh... With the attendance of over 100 vips from Evonik andother companies,the ribbon of"Evonik Specialties RadiationCuring Competence Center Asia / Pacific" was cut in its openingceremony recently held in Shanghai.This remarks an importantmilestone for Evonik to further develop its technicalcore competence of radiation curing silicone in the local marketand expand its foundational research in the region."This RC pilot will support Evonik to penetrate more 展开更多
关键词 compeTENCE SILICONE REMARKS EXPAND COMPANIES compe
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Comp.B炸药孔洞塌缩及热点生成的数值模拟
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作者 王茂杰 白桥栋 +2 位作者 韩家祥 王进平 贺全彪 《爆破器材》 北大核心 2025年第2期21-28,共8页
炸药内部孔洞塌缩机制是热点生成的主要机制。采用点火增长模型,使用S-ALE算法,在Comp.B炸药中添加微孔洞结构,研究孔洞的存在和孔洞大小对炸药起爆的影响,分析孔洞塌缩和热点生成过程。结果表明,孔洞的存在使炸药内部更容易产生热点,... 炸药内部孔洞塌缩机制是热点生成的主要机制。采用点火增长模型,使用S-ALE算法,在Comp.B炸药中添加微孔洞结构,研究孔洞的存在和孔洞大小对炸药起爆的影响,分析孔洞塌缩和热点生成过程。结果表明,孔洞的存在使炸药内部更容易产生热点,孔洞塌缩过程形成了微射流,微射流冲击孔洞下游内壁面,动能转化为内能,造成能量局部化,导致热点的形成。塌缩过程中,粒子速度达到7936 m/s,最高温度接近6000 K。初始孔洞大小决定热点的大小,孔洞越大,热点尺寸越大;孔洞大小还会影响热点温度,小孔洞的温度升高更快,而大孔洞最终的热点温度更高。 展开更多
关键词 Comp.B炸药 孔洞 塌缩机制 热点
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1例假性软骨发育不全家系报道
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作者 毕建梅 刘婷廷 +1 位作者 陆薇冰 张立琴 《中国优生与遗传杂志》 2025年第2期388-392,共5页
目的探讨1例矮身材(身高-3.3 SDS)患儿的临床表型与基因型特点并进行鉴别诊断。方法对1例矮身材患儿进行全外显子组测序(WES)分析,结合患儿临床表现、实验室检查进行诊断分析。结果以匀称性矮身材就诊患儿,临床无明显骨骼畸形,但X线摄... 目的探讨1例矮身材(身高-3.3 SDS)患儿的临床表型与基因型特点并进行鉴别诊断。方法对1例矮身材患儿进行全外显子组测序(WES)分析,结合患儿临床表现、实验室检查进行诊断分析。结果以匀称性矮身材就诊患儿,临床无明显骨骼畸形,但X线摄片表现异常,基因测序结果显示先证者COMP基因第8号外显子存在杂合变异c.817G>A,经Sanger测序验证为母源性变异,该患儿被诊断为假性软骨发育不全(PSACH)。结论本研究发现了1例以矮身材为主诉的PSACH患儿的COMP基因新发错义变异,进一步完善了PSACH的基因谱;对于严重矮身材的患儿,建议早期进行基因检测并结合临床特点、实验室检查及影像学检查结果进行诊断、鉴别诊断,避免漏诊及误诊。 展开更多
关键词 假性软骨发育不全 COMP基因 矮身材 多发性骨骺发育不良 诊断与鉴别诊断
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Giving and Receiving Compliments
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作者 Rhonda Telfer 《空中英语教室(初级版.大家说英语)》 2025年第2期37-40,55,56,共6页
Compliments are great.Maybe someone does well on a test.Praise them for their good work.Say,"Good job!"or"I'm proud of you!"Someone may have a wonderful idea.Then you can say,"That’s a gr... Compliments are great.Maybe someone does well on a test.Praise them for their good work.Say,"Good job!"or"I'm proud of you!"Someone may have a wonderful idea.Then you can say,"That’s a great idea!" 展开更多
关键词 SOMEONE limen COMP
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A hybrid uplink-downlink secure transmission scheme for UAV-aided coordinated multi-point networks
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作者 Xin Fan Guangkai Li +5 位作者 Jianqiang Li Yue Wang Chuanwen Luo Yi Hong Ting Chen Yan Huo 《Digital Communications and Networks》 2025年第3期925-936,共12页
Recognized as a pivotal facet in Beyond Fifth-Generation(B5G)and the upcoming Sixth-Generation(6G)wireless networks,Unmanned Aerial Vehicle(UAV)communications pose challenges due to limited capabilities when serving a... Recognized as a pivotal facet in Beyond Fifth-Generation(B5G)and the upcoming Sixth-Generation(6G)wireless networks,Unmanned Aerial Vehicle(UAV)communications pose challenges due to limited capabilities when serving as mobile base stations,leading to suboptimal service for edge users.To address this,the collaborative formation of Coordinated Multi-Point(CoMP)networks proves instrumental in alleviating the issue of the poor Quality of Service(QoS)at edge users in the network periphery.This paper introduces a groundbreaking solution,the Hybrid Uplink-Downlink Non-Orthogonal Multiple Access(HUD-NOMA)scheme for UAV-aided CoMP networks.Leveraging network coding and NOMA technology,our proposed HUD-NOMA effectively enhances transmission rates for edge users,notwithstanding a minor reduction in signal reception reliability for strong signals.Importantly,the system’s overall sum rate is elevated.The proposed HUD-NOMA demonstrates resilience against eavesdroppers by effectively managing intended interferences without the need for additional artificial noise injection.The study employs a stochastic geometry approach to derive the Secrecy Outage Probability(SOP)for the transmissions in the CoMP network,revealing superior performance in transmission rates and lower SOP compared to existing methods through numerical verification.Furthermore,guided by the theoretical SOP derivation,this paper proposes a power allocation strategy to further reduce the system’s SOP. 展开更多
关键词 NOMA UAV COMP Physical layer security Stochastic geometry approaches Secrecy outage probability
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La蛋白抑制剂Comp 1120通过53BP1途径增强卵巢癌细胞对卡铂的敏感性
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作者 李俊慜 朱佳蕾 汤静 《中国临床药学杂志》 2025年第2期81-86,共6页
目的探讨La蛋白抑制剂Comp 1120在增强卵巢癌细胞对卡铂的敏感性中的作用机制。方法使用A2780卵巢癌细胞株评估Comp 1120与卡铂联合使用的效果,特别关注P53结合蛋白1(53BP1)在此过程中的作用。采用Western blot、TUNEL实验和流式细胞术... 目的探讨La蛋白抑制剂Comp 1120在增强卵巢癌细胞对卡铂的敏感性中的作用机制。方法使用A2780卵巢癌细胞株评估Comp 1120与卡铂联合使用的效果,特别关注P53结合蛋白1(53BP1)在此过程中的作用。采用Western blot、TUNEL实验和流式细胞术评估细胞凋亡情况。结果Western blot分析显示,在卡铂与Comp 1120联合处理下,A2780卵巢癌细胞中促凋亡蛋白Caspase-3和BAX的表达显著上调(P<0.01),而抗凋亡蛋白Bcl-2的表达下调(P<0.01)。TUNEL实验和流式细胞术进一步证实,卡铂与Comp 1120联合使用显著促进了细胞凋亡(P<0.01)。所有实验结果均显示,这种促凋亡效应依赖于53BP1的表达;在53BP1基因沉默的细胞中,联合处理的促凋亡效应显著减弱(P<0.01),提示Comp 1120增强卡铂敏感性通过53BP1调控实现。结论La蛋白抑制剂Comp 1120能有效增强卵巢癌细胞对卡铂的敏感性,其作用机制可能涉及对53BP1途径的调节。 展开更多
关键词 LA蛋白 Comp 1120 卵巢癌 卡铂敏感性 53BP1
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Negative Cultural Transfer in English Teaching and Its Alleviating Principles
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作者 蒋晓峰 《海外英语》 2012年第2X期144-145,共2页
Teaching English as a foreign language is not tantamount to elaborating on syntactic structures or learning new vocabulary and expressions, but should incorporate some cultural elements which are intertwined with the ... Teaching English as a foreign language is not tantamount to elaborating on syntactic structures or learning new vocabulary and expressions, but should incorporate some cultural elements which are intertwined with the language itself. The main premise of the paper is that effective communication is more than a matter of linguistic proficiency and the learner's pragmatic competence is more or less arrested by the negative cultural transfer from the mother tongue, and based on that premise, the paper analyses the reasons behind negative cultural transfer from the lexical dimension, the speech act dimension, and the psychological dimension, and concludes with a tripartite toolkit of alleviating principles respectively corresponding to the classroom instruction, the teachers, and the students. 展开更多
关键词 CULTURAL TRANSFER English TEACHING PRAGMATIC compe
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一种高移动场景下基于CoMP的切换方案及性能分析 被引量:11
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作者 周祥娟 方旭明 +1 位作者 吴帆 张瑞强 《铁道学报》 EI CAS CSCD 北大核心 2013年第4期57-65,共9页
随着高速铁路的不断发展和列车速度的不断提高,高铁场景下车地通信的可靠性和高效性引起广泛关注。列车速度的提升导致更加频繁的越区切换,使系统的可靠性得不到保障,因此列车速度的提升对高铁通信网络的安全性和可靠性产生严重影响。... 随着高速铁路的不断发展和列车速度的不断提高,高铁场景下车地通信的可靠性和高效性引起广泛关注。列车速度的提升导致更加频繁的越区切换,使系统的可靠性得不到保障,因此列车速度的提升对高铁通信网络的安全性和可靠性产生严重影响。本文提出1种将LTE-A(Long Term Evolution-Advanced)网络应用到高速铁路场景并基于协同多点传输/接收CoMP(Coordinated Multi-Point transmission/reception)技术的切换优化方案模型,方案参考协作集加入模型。对系统切换成功率等进行性能分析,并与传统切换方案进行性能对比。分析表明:本文基于CoMP的切换方案可有效提升高移动性下网络的切换成功概率,明显改善系统的可靠性和效率。 展开更多
关键词 高移动性 LTE-A 切换 COMP 切换成功率 中断率
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基于MATLAB的LTE仿真平台CoMP功能的设计与实现 被引量:8
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作者 周文安 陈微 +2 位作者 陈思 谭中一 李慧芹 《系统仿真学报》 CAS CSCD 北大核心 2012年第11期2259-2263,共5页
以维也纳技术大学的LTE系统级仿真平台为基础,设计实现了多点协作传输(CoMP)功能。在原仿真平台上增加了三个模块:边缘用户判断模块,协作小区选择模块和资源分配模块。新增加的三个模块使用SINR门限法来判断边缘用户,采用用户选择协作... 以维也纳技术大学的LTE系统级仿真平台为基础,设计实现了多点协作传输(CoMP)功能。在原仿真平台上增加了三个模块:边缘用户判断模块,协作小区选择模块和资源分配模块。新增加的三个模块使用SINR门限法来判断边缘用户,采用用户选择协作小区方案以提高仿真平台的CoMP性能,并提出了新的资源分配方法解决了用户选择协作小区带来的资源分配冲突问题。模块化的设计使得平台可用自带的初始配置文件对三个模块进行统一配置,实现了与原平台的兼容,又降低了与原平台的耦合性,增加了可扩展性。此外增加了基站与用户进行CoMP时需要相互交互的信令。还增加了用户运动的动画演示,能直观有效地观察用户的移动和基站对用户进行CoMP的情况。最后对该CoMP功能进行仿真测试,结果与3GPP相关提案基本一致,证明了此功能的正确性和有效性。 展开更多
关键词 LTE 仿真平台 COMP MATLAB
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黄芪甲苷对Wnt/β-catenin信号通路活化的人滑膜及软骨细胞共培养体系相关因子的调控作用 被引量:16
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作者 王德刚 许传勇 +7 位作者 姜玉祥 阚卫兵 肖志锋 姚丽 刘婉 谢殿洪 汤豪 张玉婷 《广东医学》 CAS 北大核心 2017年第3期354-357,共4页
目的观察黄芪甲苷对Wnt/β-catenin信号通路活化的人滑膜细胞和人软骨细胞共培养体系相关因子的调控作用,初步阐释黄芪甲苷治疗骨关节炎的分子作用机制。方法通过β-catenin慢病毒载体转染正常人滑膜细胞,激活其Wnt/β-catenin信号通路... 目的观察黄芪甲苷对Wnt/β-catenin信号通路活化的人滑膜细胞和人软骨细胞共培养体系相关因子的调控作用,初步阐释黄芪甲苷治疗骨关节炎的分子作用机制。方法通过β-catenin慢病毒载体转染正常人滑膜细胞,激活其Wnt/β-catenin信号通路。Wnt/β-catenin信号通路活化的人滑膜细胞与正常人软骨细胞置于Transwell小室中共培养。黄芪甲苷混悬液干预共培养体系,采用ELISA方法检测黄芪甲苷对滑膜细胞、软骨细胞培养上清液基质金属蛋白酶-7(MMP-7)、Ⅱ型胶原羧基端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)表达的影响。结果黄芪甲苷干预后,ELISA法检测发现滑膜与软骨细胞上清液中MMP-7、CTX-Ⅱ、COMP表达与正常对照相比明显升高(P<0.01)。结论黄芪甲苷可以抑制滑膜细胞Wnt/β-catenin信号通路,下调MMP-7、CTX-Ⅱ、COMP的表达。黄芪甲苷可能通过抑制骨关节炎滑膜Wnt/β-catenin信号通路,改善滑膜炎症、调控滑膜-软骨共同体系微环境、达到抑制软骨降解,促进软骨细胞功能恢复而治疗骨关节炎。 展开更多
关键词 Wnt信号通路 细胞共培养 MMP-7、CTX-Ⅱ、COMP 黄芪甲苷
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射弹冲击带盖板Comp B装药起爆过程数值模拟 被引量:12
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作者 崔凯华 洪滔 曹结东 《含能材料》 EI CAS CSCD 北大核心 2010年第3期286-289,共4页
分析并验证了圆柱形平头铜射弹冲击起爆带盖板Comp B装药,利用LS-DYNA模拟了圆柱形平头和圆头钨射弹冲击带不同厚度钢盖板的Comp B装药起爆过程,定量地分析了起爆临界速度随盖板厚度的变化规律,讨论了发生延迟起爆(XDT)现象的原因和机... 分析并验证了圆柱形平头铜射弹冲击起爆带盖板Comp B装药,利用LS-DYNA模拟了圆柱形平头和圆头钨射弹冲击带不同厚度钢盖板的Comp B装药起爆过程,定量地分析了起爆临界速度随盖板厚度的变化规律,讨论了发生延迟起爆(XDT)现象的原因和机理。结果表明,直接冲击起爆计算结果能够较好地符合Jacobs判据,圆头与平头钨射弹冲击带盖板Comp B装药临界起爆速度满足关系式Vround≈1.15Vflat,发生XDT现象是因为炸药被破坏及反射冲击共同作用所致,且XDT现象产生位置均在邻近底板的炸药界面处。 展开更多
关键词 爆炸力学 射弹 COMP B装药 冲击起爆 延迟起爆 临界速度
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基于J-C本构模型的Comp.B炸药落锤冲击数值模拟 被引量:13
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作者 李凯 朱建生 +2 位作者 钱志博 董树南 倪添 《力学与实践》 CSCD 北大核心 2011年第1期21-23,73,共4页
为研究Comp.B炸药在惯性冲击下的力学响应特性,基于Johnson-Cook本构模型,对点火前的Comp.B炸药大落锤(400 kg)冲击实验进行了数值模拟.在不同应力加载条件下模拟计算得到了用状态方程和不用状态方程的σ-t曲线,对比应力峰值、应力上升... 为研究Comp.B炸药在惯性冲击下的力学响应特性,基于Johnson-Cook本构模型,对点火前的Comp.B炸药大落锤(400 kg)冲击实验进行了数值模拟.在不同应力加载条件下模拟计算得到了用状态方程和不用状态方程的σ-t曲线,对比应力峰值、应力上升时间等主要力学参数,结果表明考虑了状态方程的数值模拟结果与实验吻合最好,可以很好地模拟炸药惯性冲击下的力学响应. 展开更多
关键词 J-C模型 Comp.B炸药 落锤冲击实验
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Effects of High Light Stress on Chlorophyll-protein Complexes of Two Subspecies of Rice 被引量:10
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作者 辛越勇 冯丽洁 +3 位作者 许亦农 焦德茂 李良璧 匡廷云 《Acta Botanica Sinica》 CSCD 2000年第12期1278-1284,共7页
Influence of high light stress on the photosynthesis of flag leaves of indica subspecies (cv. “Shanyou 63', sensitive to photoinhibition) and japonica subspecies (cv. “Wuyujing', resistant to photoin... Influence of high light stress on the photosynthesis of flag leaves of indica subspecies (cv. “Shanyou 63', sensitive to photoinhibition) and japonica subspecies (cv. “Wuyujing', resistant to photoinhibition) of rice ( Oryza sativa L.) was comparatively investigated. In both cultivars of rice, the excitation energy distribution between two photosystems was altered and the excitation energy transfer from light harvesting chlorophyll protein complexes to PSⅡ was inhibited by high light stress. These decreases were more pronounced in indica rice cultivar as compared to japonica one. The analysis of mild SDS_PAGE showed that in indica rice, high light stress almost disaggregated the trimer of light harvesting chlorophyll protein complexes of PSⅡ (LHC Ⅱ 1). The stress reduced the contents of internal antennae chlorophyll protein complexes of PSⅡ (CPa), light harvesting chlorophyll protein of PSⅠ (CPⅠa) and Chl a protein complex of PSⅠ reaction center (CPⅠ) as well as dimer of LHCⅡ (LHCⅡ 2) in indica rice. In japonica subspecies, however, high light stress depressed the contents of LHCⅡ 1, CPa and CPⅠa, but slightly impacted on CPⅠ content. Moreover, the increase in the contents of monomer of LHCⅡ by high light stress was found in both subspecies. In consistent with above results, analysis of polypeptide indicated that the amounts of 27 kD and 25 kD polypeptide of LHCⅡ in particular, as well as that of 21 kD polypeptide of CPⅠa were reduced by high light stress in both subspecies. It was found that, comparing with japonica rice, the stress pronouncedly diminished 43 kD and 47 kD proteins of CPa and 23 kD extrisic protein in indica rice. 展开更多
关键词 high light stress subspecies of rice excitation energy distribution chlorophyll_protein comp(
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LTE的新技术展望 被引量:6
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作者 邵震 曹敏 李一明 《电信科学》 北大核心 2013年第2期8-12,18,共6页
为应对移动互联网带来的海量数据流量的承载需求,4G网络亟需引入异构网的新型组网形态,并打造多网络智能协同融合的移动智能管道,争取首先在组网架构上有一个新突破。本文基于3GPP标准化进程,对LTE分层组网的HetNet、小区干扰协调以及LT... 为应对移动互联网带来的海量数据流量的承载需求,4G网络亟需引入异构网的新型组网形态,并打造多网络智能协同融合的移动智能管道,争取首先在组网架构上有一个新突破。本文基于3GPP标准化进程,对LTE分层组网的HetNet、小区干扰协调以及LTE与WLAN智能协同的SaMOG、ANDSF等新技术进行展望。 展开更多
关键词 HetNet eICIC COMP SaMOG ANDSF
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塞来昔布对人滑膜细胞和人软骨细胞共培养体系相关因子的调控作用 被引量:4
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作者 王德刚 许传勇 +7 位作者 姜玉祥 阚卫兵 肖志锋 姚丽 刘婉 谢殿洪 汤豪 张玉婷 《广东医学》 CAS 北大核心 2016年第9期1278-1282,共5页
目的观察塞来昔布对Wnt/β-catenin信号通路活化的人滑膜细胞和人软骨细胞共培养体系相关因子的调控作用,初步阐释塞来昔布治疗骨关节炎的分子作用机制。方法通过β-catenin慢病毒载体转染正常人滑膜细胞,激活其Wnt/β-catenin信号通路... 目的观察塞来昔布对Wnt/β-catenin信号通路活化的人滑膜细胞和人软骨细胞共培养体系相关因子的调控作用,初步阐释塞来昔布治疗骨关节炎的分子作用机制。方法通过β-catenin慢病毒载体转染正常人滑膜细胞,激活其Wnt/β-catenin信号通路。Wnt/β-catenin信号通路活化的人滑膜细胞与正常人软骨细胞置于Transwell小室中共培养。倒置显微镜观察Wnt/β-catenin信号通路活化的人滑膜细胞对人软骨细胞形态学变化的影响。塞来昔布混悬液干预共培养体系,采用Western blot方法检测塞来昔布对滑膜细胞β-catenin蛋白表达的影响;采用ELISA方法检测塞来昔布对滑膜细胞、软骨细胞培养上清液基质金属蛋白酶-7(MMP-7)、Ⅱ型胶原羧基端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)表达的影响。结果β-catenin慢病毒载体转染正常人滑膜细胞后成功激活Wnt信号通路并与软骨细胞共培养。随着培养时间的延长,软骨细胞生长逐渐受到抑制,软骨细胞胞体边缘与板壁接触处发白,折光度增加,细胞贴壁强度降低。塞来昔布干预后,Western blot检测发现滑膜细胞中β-catenin蛋白的表达明显下调(P<0.01);ELISA法检测发现滑膜与软骨细胞上清液中MMP-7、CTX-Ⅱ、COMP的表达与正常对照相比明显升高(P<0.01)。结论滑膜细胞Wnt/β-catenin信号通路的激活可以使软骨细胞生长受到抑制。塞来昔布可以降低滑膜细胞β-catenin表达,且与干预时间长短相关。塞来昔布可以抑制滑膜细胞Wnt/β-catenin信号通路,下调MMP-7、CTX-Ⅱ、COMP的表达。塞来昔布可能通过抑制OA滑膜Wnt/β-catenin信号通路,改善滑膜炎症、调控滑膜-软骨共同体系微环境、达到抑制软骨降解,促进软骨细胞功能恢复而治疗骨关节炎。 展开更多
关键词 Wnt信号通路 细胞共培养 MMP-7 CTX-Ⅱ COMP 塞来昔布
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基质金属蛋白酶ADAMTS的研究进展 被引量:7
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作者 魏凡华 张玉颖 于修平 《中国细胞生物学学报》 CAS CSCD 北大核心 2014年第8期1186-1192,共7页
ADAMTS是含有TSP基序的分泌性多结构域蛋白水解酶。ADAMTS基因的突变或过表达与多种生理学和病理学过程相关。ADAMTS基因的过表达促进了细胞外基质成分的降解,加速了关节炎和动脉粥样硬化的疾病进程。ADAMTS基因的突变则与肿瘤的生长和... ADAMTS是含有TSP基序的分泌性多结构域蛋白水解酶。ADAMTS基因的突变或过表达与多种生理学和病理学过程相关。ADAMTS基因的过表达促进了细胞外基质成分的降解,加速了关节炎和动脉粥样硬化的疾病进程。ADAMTS基因的突变则与肿瘤的生长和侵袭以及遗传性发育紊乱等密切相关。该文将结合作者的研究工作,重点对ADAMTS-7的当前研究概况进行综述,讨论其结构、功能、调节及其在相关炎性疾病中的作用等。 展开更多
关键词 ADAMTS 金属蛋白酶 关节炎 细胞外基质 COMP
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