The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicoti...The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.展开更多
The fracturing of the pressure compartments in sedimentary basins may not be caused completely by natural hydraulic fracturing. On the bases of fracture mechanics and numerical simulation, we consider that there are t...The fracturing of the pressure compartments in sedimentary basins may not be caused completely by natural hydraulic fracturing. On the bases of fracture mechanics and numerical simulation, we consider that there are two mechanisms concerning the fracturing of pressure compartments, which are as follows: (1) natural hydraulic fracturing of the sediments within pressure compartments, and (2) tensile fracturing due to tangential traction. When the fracture, formed in the sediments within pressure compartments due to hydraulic fracturing, arrives at the base of the seal, the intensely tangential tensile stresses are created and lead to the fracturing of the seal. After the seal fractured, the fluid escaped from the pressure compartments. In a shallow pressure compartment, the duration of fracture opening and fluid expulsion is about 10-20 years in a cycle.展开更多
Strictosidine synthase (STR) is a key enzyme involved in the biosynthesis of terpenoid indole alkaloids (TIA) by condensing tryptamine and secologanin into strictosidine. The transgenic tobacco plants targeting STR to...Strictosidine synthase (STR) is a key enzyme involved in the biosynthesis of terpenoid indole alkaloids (TIA) by condensing tryptamine and secologanin into strictosidine. The transgenic tobacco plants targeting STR to subcellular compartments were established to express STR in chloroplast, vacuole and endoplasmic reticulum (ER) by the tobacco stable transformation. It was shown that STR was effectively expressed in the above subcellular compartments by Western blot analysis and STR enzymatic assay. In vitro , STR enzymatic assay was measured indirectly by fluorimetrically detecting depletion of tryptamine feeding on secologanin in the reaction mixture. The tryptamine were completely depleted by STR in the crude extract of leaves of transgenic tobacco plants targeting and expressing STR in the chloroplast, vacuole and ER, which ascertained the STR functionally targeted to the three subcellular compartments. To confirm STR correct targeting and expressing in chloroplast, the chloroplasts were isolated and the fractions of purified chloroplasts were analyzed by Western blot. The hypothesis of STR correct targeting to the chloroplast was tested. The results have implications on our understanding of the complex intracellular trafficking in metabolic intermediates of TIA biosynthesis.展开更多
The SEIR epidemic model studied here includes constant inflows of new susceptibles, exposeds, infectives, and recovereds. This model also incorporates a population size dependent contact rate and a disease-related dea...The SEIR epidemic model studied here includes constant inflows of new susceptibles, exposeds, infectives, and recovereds. This model also incorporates a population size dependent contact rate and a disease-related death. As the infected fraction cannot be eliminated from the population, this kind of model has only the unique endemic equilibrium that is globally asymptotically stable. Under the special case where the new members of immigration are all susceptible, the model considered here shows a threshold phenomenon and a sharp threshold has been obtained. In order to prove the global asymptotical stability of the endemic equilibrium, the authors introduce the change of variable, which can reduce our four-dimensional system to a three-dimensional asymptotical autonomous system with limit equation.展开更多
Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be...Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.展开更多
Striated muscle tissue contains fibers with high oxidative capacity (heart muscle), higher oxidative capacity (type I and IIA fibers of skeletal muscle) and low oxidative capacity (type IIB/X fibers of skeletal muscle...Striated muscle tissue contains fibers with high oxidative capacity (heart muscle), higher oxidative capacity (type I and IIA fibers of skeletal muscle) and low oxidative capacity (type IIB/X fibers of skeletal muscle). Muscle fibers with higher oxidative capacity contain large mitochondria tightly packed with cristae as well as small forms of mitochondria containing relatively few cristae. The intensive development of the mitochondrial apparatus in the post-activity period reflects the adaptive processes, which is intended to supply the increased energy requirements of muscle fibers with higher oxidative capacity. Muscle fibers with low oxidative capacity contain significantly less mitochondria than fibers with higher capacity. It is typical to type IIB fibers that after intensive muscle activity there are damaged myofibrils in a relatively small area, some myofibrils are twisted and lose the connection with the neighboring structures. It is still not fully known how skeletal muscles with different oxidative capacity respond to an increased functional activity and what differences exist in these fibers between oxidative capacity and function of myofibrils. The aim of the present short review was to compare structural-functional changes in mitochondrial and myofibrillar compartments of heart and skeletal muscle fibers with different oxidative capacity and the effect of increased functional activity on the interaction of these compartments.展开更多
The structure of an air-floating caisson is suitable for the major structure of caisson-type artificial islands.Thus,it has been rapidly developed and widely used in the exploration and development of oil and gas fiel...The structure of an air-floating caisson is suitable for the major structure of caisson-type artificial islands.Thus,it has been rapidly developed and widely used in the exploration and development of oil and gas fields in shallow sea and intertidal zones.Air-floating transportation technology is one of the key technologies employed in this structure.In this paper,the factors influencing the dynamic response characteristics of air-floating caisson with multi-compartments(AFCMC)were studied using model tests.The length and the height of each air-floating structure in the model were 1.0 and 0.1 m,respectively.In addition,the 1:100 models with 6,8,and 10 compartments under regular waves were tested in the wave flume,respectively.In the experiments,the respective water depths were set at 0.2,0.3,and 0.4 m,and the corresponding drafts were 0.05,0.06,and 0.07 m.Results show that with the increase of draft,the heave natural period increased and the maximum amplitude of the heave motion decreased.Meanwhile,the pitch motion decreased at 6 and 8 compartments and increased at 10 compartments.As the water depth increased,the maximum amplitude and amplitude change of heave and pitch motions first increased and then decreased.However,several amplitudes close to the maximum amplitude appeared in the measured period at shallower water depth,thereby indicating the vertical movements of the structure enhanced under shallow water.The increase in the number of compartments reduced the vertical movements under 6.0 m draft,but it increased the vertical movements under 5.0 and 7.0 m draft.Thus,increasing the number of compartments has a limited capacity to improve the motion performance of the structure.展开更多
The implementation of early and accurate detection of aircraft cargo compartment fire is of great significance to ensure flight safety.The current airborne fire detection technology mostly relies on single-parameter s...The implementation of early and accurate detection of aircraft cargo compartment fire is of great significance to ensure flight safety.The current airborne fire detection technology mostly relies on single-parameter smoke detection using infrared light.This often results in a high false alarm rate in complex air transportation envi-ronments.The traditional deep learning model struggles to effectively address the issue of long-term dependency in multivariate fire information.This paper proposes a multi-technology collaborative fire detection method based on an improved transformers model.Dual-wavelength optical sensors,flue gas analyzers,and other equipment are used to carry out multi-technology collaborative detection methods and characterize various feature dimensions of fire to improve detection accuracy.The improved Transformer model which integrates the self-attention mechanism and position encoding mechanism is applied to the problem of long-time series modeling of fire information from a global perspective,which effectively solves the problem of gradient disappearance and gradient explosion in traditional RNN(recurrent neural network)and CNN(convolutional neural network).Two different multi-head self-attention mechanisms are used to classify and model multivariate fire information,respectively,which solves the problem of confusing time series modeling and classification modeling in dealing with multivariate classification tasks by a single attention mechanism.Finally,the output results of the two models are fused through the gate mechanism.The research results show that,compared with the traditional single-feature detection technology,the multi-technology collaborative fire detection method can better capture fire information.Compared with the traditional deep learning model,the multivariate fire pre-diction model constructed by the improved Transformer can better detect fires,and the accuracy rate is 0.995.展开更多
Contagious pathogens like COVID-19 transmitted via respiratory droplets spread effortlessly in the passenger compartments of transport,significantly jeopardizing passengers’safety when taking public transportation.To...Contagious pathogens like COVID-19 transmitted via respiratory droplets spread effortlessly in the passenger compartments of transport,significantly jeopardizing passengers’safety when taking public transportation.To date,studies on the fundamental theories of airborne droplet transmission and the engineering application of decontamination techniques are insufficient for the prevention and control of pathogens transmitting in the compartments of passenger transport.It is essential to systematically investigate the control approaches to restrain pathogens from transmitting in passenger compartments.Herein,a theoretical framework for calculating the transmission of pathogens in a complex compartment environment was proposed,and experimental platforms that satisfy the Biosafety Level-2 Laboratory safety level for compartment environment simulations were built based on a set of real train cabins.On these bases,numerical investigations on the motion of pathogen-laden droplets were conducted,and decontamination techniques were examined experimentally.Thereby,control measures on the pathogen transmission and pathogen decontamination schemes were proposed.Moreover,highly efficient decontamination devices were developed,and coping strategies for epidemic emergencies were devised.The outcomes provide theoretical and technical support for developing the next generation of transportation and the prevention and control measures cooperatively considering regular and pandemic times.展开更多
This study investigates the wet towing characteristics of an integrated wellhead platform supported by a bucket foundation.These characteristics are crucial for optimizing offshore construction efficiency and enabling...This study investigates the wet towing characteristics of an integrated wellhead platform supported by a bucket foundation.These characteristics are crucial for optimizing offshore construction efficiency and enabling the development of small marginal oil fields.The wet towing behavior of the integrated wellhead platform was explored through a combination of physical experiments and numerical simulations.Physical experiments were conducted to validate the accuracy of the numerical simulations.Subsequently,numerical simulations were employed to determine the impacts of towing speed and wave direction on the towing process of the integrated wellhead platform.Finally,the impact of compartment failure due to bulkhead damage on towage stability was analyzed.Findings indicate that the wave and towing directions influence the motion of the platform at various degrees of freedom.The platform demonstrates optimal stability when towing against waves,particularly when the towing direction aligns at a specific angle relative to the wave direction.In addition,a controlled increase in towing speed within a specific range effectively mitigates roll and pitch motions,which enhances the overall tow stability of the platform.Notably,compartment failure has an adverse effect on the towing stability,particularly in aft compartments.Therefore,it requires careful consideration and attention in practical engineering scenarios.展开更多
The muzzle blast overpressure induces disturbances in the flow field inside the crew compartment(FFICC)of a truck-mounted howitzer during the artillery firing.This overpressure is the primary factor preventing personn...The muzzle blast overpressure induces disturbances in the flow field inside the crew compartment(FFICC)of a truck-mounted howitzer during the artillery firing.This overpressure is the primary factor preventing personnel from firing artillery within the cab.To investigate the overpressure characteristics of the FFICC,a foreign trade equipment model was used as the research object,and a numerical model was established to analyze the propagation of muzzle blast from the muzzle to the interior of the crew compartment under extreme firing condition.For comparative verification,the muzzle blast experiment included overpressure data from both the flow field outside the crew compartment(FFOCC)and the FFICC,as well as the acceleration data of the crew compartment structure(Str-CC).The research findings demonstrate that the overpressure-time curves of the FFICC exhibit multi-peak characteristics,while the pressure wave shows no significant discontinuity.The enclosed nature of the cab hinders the dissipation of pressure wave energy within the FFICC,leading to sustained high-amplitude overpressure.The frameskin structure helps attenuate the impact of muzzle blast on the FFICC.Conversely,local high overpressure caused by the convex or concave features of the cab's exterior significantly amplifies the overpressure amplitude within the FFICC.展开更多
Acute compartment syndrome(ACoS)involves a complex pathological process in which rising pressures within an enclosed muscle space ultimately result in anoxia and cell death.The most common etiologies include long bone...Acute compartment syndrome(ACoS)involves a complex pathological process in which rising pressures within an enclosed muscle space ultimately result in anoxia and cell death.The most common etiologies include long bone fractures,crush injuries,and burns.[1,2]While rare,ACoS can also occur in atraumatic settings following periods of prolonged limb compression,such as intoxication.[3]Atraumatic ACoS is easily overlooked,resulting in diagnostic delay and poor patient outcomes.Prompt recognition and surgical management is essential to prevent critical sequelae.展开更多
BACKGROUND Hypoganglionosis is a rare gastrointestinal acquired motility disorder that res-embles Hirschsprung’s disease and can manifest in the adult life.Abdominal compartment syndrome,a condition characterized by ...BACKGROUND Hypoganglionosis is a rare gastrointestinal acquired motility disorder that res-embles Hirschsprung’s disease and can manifest in the adult life.Abdominal compartment syndrome,a condition characterized by an increase in intra-abdo-minal pressure with physiological disturbance can be caused by severe massive fecal impaction.CASE SUMMARY A 33-year-old female presented to the emergency room with massive abdominal distension that rapidly progressed to abdominal compartment syndrome.The pa-tient was diagnosed with hypoganglionosis.Life-saving emergent proctoco-lectomy was performed to save the patient.CONCLUSION Abdominal compartment syndrome can develop secondary to excessive colonic distension.This extreme but rare situation must be addressed immediately.Hypo-ganglionosis is a potential cause of severe constipation that may present in adu-lthood.展开更多
The spatial organization of the genome plays an important role in the regulation of gene expression. How- ever, the core structural features of animal genomes, such as topologically associated domains （TADs） and chr...The spatial organization of the genome plays an important role in the regulation of gene expression. How- ever, the core structural features of animal genomes, such as topologically associated domains （TADs） and chromatin loops, are not prominent in the extremely compact Arabidopsis genome. In this study, we examine the chromatin architecture, as well as their DNA methylation, histone modifications, accessible chromatin, and gene expression, of maize, tomato, sorghum, foxtail millet, and rice with genome sizes ranging from 0.4 to 2.4 Gb. We found that these plant genomes can be divided into mammalian-like A/B compartments. At higher resolution, the chromosomes of these plants can be further partitioned to local AJB compartments that reflect their euchromatin, heterochromatin, and polycomb status. Chromatins in all these plants are organized into domains that are not conserved across species. They show similarity to the Drosophila compartment domains, and are clustered into active, polycomb, repressive, and interme- diate types based on their transcriptional activities and epigenetic signatures, with domain border overlaps with the local A/B compartment junctions. In the large maize and tomato genomes, we observed extensive chromatin loops. However, unlike the mammalian chromatin loops that are enriched at the TAD border, plant chromatin loops are often formed between gene islands outside the repressive domains and are closely associated with active compartments. Our study indicates that plants have complex and unique 3D chromatin architectures, which require further study to elucidate their biological functions.展开更多
Prevacuolar compartments （PVCs） and endosomal compartments are membrane-bound organelles mediating protein traffic to vacuoles in the secretory and endocytic pathways of plant cells. Over the years, great progress h...Prevacuolar compartments （PVCs） and endosomal compartments are membrane-bound organelles mediating protein traffic to vacuoles in the secretory and endocytic pathways of plant cells. Over the years, great progress has been made towards our understanding in these two compartments in plant cells. In this review, we will summarize our contributions toward the identification and characterization of plant prevacuolar and endosomal compartments. Our studies will serve as important steps in future molecular characterization of PVC biogenesis and PVC-mediated protein traffickinq in plant cells.展开更多
In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these architectures remain incompletely ...In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these architectures remain incompletely understood. Here, we investigated the functional roles of H3 lysine 9 dimethylation(H3 K9 me2), one of the abundant histone modifications, in three-dimensional(3 D)genome organization. Unlike in mouse embryonic stem cells, inhibition of methyltransferases G9 a and GLP in differentiated cells eliminated H3 K9 me2 predominantly at A-type(active) genomic compartments, and the level of residual H3 K9 me2 modifications was strongly associated with B-type(inactive) genomic compartments. Furthermore, chemical inhibition of G9 a/GLP in mouse hepatocytes led to decreased chromatin-nuclear lamina interactions mainly at G9 a/GLP-sensitive regions, increased degree of genomic compartmentalization, and up-regulation of hundreds of genes that were associated with alterations of the 3 D chromatin. Collectively, our data demonstrated essential roles of H3 K9 me2 in 3 D genome organization.展开更多
Deterministic compartment models(CMs)and stochastic models,including stochastic CMs and agent-based models,are widely utilized in epidemic modeling.However,the relationship between CMs and their corresponding stochast...Deterministic compartment models(CMs)and stochastic models,including stochastic CMs and agent-based models,are widely utilized in epidemic modeling.However,the relationship between CMs and their corresponding stochastic models is not well understood.The present study aimed to address this gap by conducting a comparative study using the susceptible,exposed,infectious,and recovered(SEIR)model and its extended CMs from the coronavirus disease 2019 modeling literature.We demonstrated the equivalence of the numerical solution of CMs using the Euler scheme and their stochastic counterparts through theoretical analysis and simulations.Based on this equivalence,we proposed an efficient model calibration method that could replicate the exact solution of CMs in the corresponding stochastic models through parameter adjustment.The advancement in calibration techniques enhanced the accuracy of stochastic modeling in capturing the dynamics of epidemics.However,it should be noted that discrete-time stochastic models cannot perfectly reproduce the exact solution of continuous-time CMs.Additionally,we proposed a new stochastic compartment and agent mixed model as an alternative to agent-based models for large-scale population simulations with a limited number of agents.This model offered a balance between computational efficiency and accuracy.The results of this research contributed to the comparison and unification of deterministic CMs and stochastic models in epidemic modeling.Furthermore,the results had implications for the development of hybrid models that integrated the strengths of both frameworks.Overall,the present study has provided valuable epidemic modeling techniques and their practical applications for understanding and controlling the spread of infectious diseases.展开更多
BACKGROUND Unicompartmental knee arthroplasty(UKA)and high tibial osteotomy(HTO)are well-established operative interventions in the treatment of knee osteoarthritis.However,which intervention is more beneficial to pat...BACKGROUND Unicompartmental knee arthroplasty(UKA)and high tibial osteotomy(HTO)are well-established operative interventions in the treatment of knee osteoarthritis.However,which intervention is more beneficial to patients with knee osteoarthritis remains unknown and a topic of much debate.Simultaneously,there is a paucity of research assessing the relationship between radiographic parameters of knee joint alignment and patient-reported clinical outcomes,preoperatively and following HTO or UKA.AIM To compare UKAs and HTOs as interventions for medial-compartment knee osteoarthritis:Examining differences in clinical outcome and investigating the relationship of joint alignment with respect to this.METHODS This longitudinal observational study assessed a total of 42 patients that had undergone UKA(n=23)and HTO(n=19)to treat medial compartment knee osteoarthritis.Patient-reported outcome measures(PROMs)were collected to evaluate clinical outcome.These included two disease-specific(Knee Injury and Osteoarthritis Outcome Score,Oxford Knee Score)and two generic(EQ-5D-5L,Short Form-12)PROMs.The radiographic parameters of knee alignment assessed were the:Hip-knee-ankle angle,mechanical axis deviation and angle of Mikulicz line.RESULTS Statistical analyses demonstrated significant(P<0.001),preoperative to postoperative,improvements in the PROM scores of both groups.There were,however,no significant inter-group differences in the postoperative PROM scores of the UKA and HTO group.Several significant correlations associated a more distolaterally angled Mikulicz line with worse knee function and overall health preoperatively(P<0.05).Postoperatively,two clusters of significant correlations were observed between the disease-specific PROM scores and knee joint alignment parameters(hip-knee-ankle angle,mechanical axis deviation)within the HTO group;yet no such associations were observed within the UKA group.CONCLUSION UKAs and HTOs are both efficacious operations that provide a comparable degree of clinical benefit to patients with medial compartment knee osteoarthritis.Clinical outcome has a limited association with radiographic parameters of knee joint alignment postoperatively;however,a more distolaterally angled Mikulicz line appears associated with worse knee function/health-related quality of life preoperatively.展开更多
We suggest that during severe acute pancreatitis(SAP)with intra-abdominal hypertension,practitioners should consider decompressive laparotomy,even with intra-abdominal pressure(IAP)below 25 mmHg.Indeed,in this setting...We suggest that during severe acute pancreatitis(SAP)with intra-abdominal hypertension,practitioners should consider decompressive laparotomy,even with intra-abdominal pressure(IAP)below 25 mmHg.Indeed,in this setting,non-occlusive mesenteric ischemia(NOMI)may occur even with IAP below this cutoff and lead to transmural necrosis if abdominal perfusion pressure is not promptly restored.We report our experience of 18 critically ill patients with SAP having undergone decompressive laparotomy of which one third had NOMI while IAP was mostly below 25 mmHg.展开更多
Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant ener...Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake. As a result, goose offers an excellent model for studying obesity, metabolic disorders, and liver diseases in mammals. Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged, the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.Results In this study, geese exhibited more pronounced changes in the liver index and triglyceride(TG) content following the consumption of the high-fat diet(HFD) than mice without significant signs of inflammation. Additionally, we performed comprehensive analyses on 10 goose liver tissues(5 HFD, 5 normal), including generating highresolution maps of chromatin architecture, conducting whole-genome gene expression profiling, and identifying H3K27ac peaks in the livers of geese and mice subjected to the HFD. Our results unveiled a multiscale restructuring of chromatin architecture, encompassing Compartment A/B, topologically associated domains, and interactions between promoters and enhancers. The dynamism of the three-dimensional genome architecture, prompted by the HFD, assumed a pivotal role in the transcriptional regulation of crucial genes. Furthermore, we identified genes that regulate chromatin conformation changes, contributing to the metabolic adaptation process of lipid deposition and hepatic fat changes in geese in response to excessive energy intake. Moreover, we conducted a cross-species analysis comparing geese and mice exposed to the HFD, revealing unique characteristics specific to the goose liver compared to a mouse. These chromatin conformation changes help elucidate the observed characteristics of fat deposition and hepatic fat regulation in geese under conditions of excessive energy intake.Conclusions We examined the dynamic modifications in three-dimensional chromatin architecture and gene expression induced by an HFD in goose liver tissues. We conducted a cross-species analysis comparing that of mice. Our results contribute significant insights into the chromatin architecture of goose liver tissues, offering a novel perspective for investigating mammal liver diseases.展开更多
文摘The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.
文摘The fracturing of the pressure compartments in sedimentary basins may not be caused completely by natural hydraulic fracturing. On the bases of fracture mechanics and numerical simulation, we consider that there are two mechanisms concerning the fracturing of pressure compartments, which are as follows: (1) natural hydraulic fracturing of the sediments within pressure compartments, and (2) tensile fracturing due to tangential traction. When the fracture, formed in the sediments within pressure compartments due to hydraulic fracturing, arrives at the base of the seal, the intensely tangential tensile stresses are created and lead to the fracturing of the seal. After the seal fractured, the fluid escaped from the pressure compartments. In a shallow pressure compartment, the duration of fracture opening and fluid expulsion is about 10-20 years in a cycle.
文摘Strictosidine synthase (STR) is a key enzyme involved in the biosynthesis of terpenoid indole alkaloids (TIA) by condensing tryptamine and secologanin into strictosidine. The transgenic tobacco plants targeting STR to subcellular compartments were established to express STR in chloroplast, vacuole and endoplasmic reticulum (ER) by the tobacco stable transformation. It was shown that STR was effectively expressed in the above subcellular compartments by Western blot analysis and STR enzymatic assay. In vitro , STR enzymatic assay was measured indirectly by fluorimetrically detecting depletion of tryptamine feeding on secologanin in the reaction mixture. The tryptamine were completely depleted by STR in the crude extract of leaves of transgenic tobacco plants targeting and expressing STR in the chloroplast, vacuole and ER, which ascertained the STR functionally targeted to the three subcellular compartments. To confirm STR correct targeting and expressing in chloroplast, the chloroplasts were isolated and the fractions of purified chloroplasts were analyzed by Western blot. The hypothesis of STR correct targeting to the chloroplast was tested. The results have implications on our understanding of the complex intracellular trafficking in metabolic intermediates of TIA biosynthesis.
基金This research is supported by the NNSF of China (19971066)
文摘The SEIR epidemic model studied here includes constant inflows of new susceptibles, exposeds, infectives, and recovereds. This model also incorporates a population size dependent contact rate and a disease-related death. As the infected fraction cannot be eliminated from the population, this kind of model has only the unique endemic equilibrium that is globally asymptotically stable. Under the special case where the new members of immigration are all susceptible, the model considered here shows a threshold phenomenon and a sharp threshold has been obtained. In order to prove the global asymptotical stability of the endemic equilibrium, the authors introduce the change of variable, which can reduce our four-dimensional system to a three-dimensional asymptotical autonomous system with limit equation.
基金supported financially by the National Key Research and Development Program of China(2021YFD1700200)the China Agriculture Research System of MOF and MARA(CARS-22).
文摘Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.
文摘Striated muscle tissue contains fibers with high oxidative capacity (heart muscle), higher oxidative capacity (type I and IIA fibers of skeletal muscle) and low oxidative capacity (type IIB/X fibers of skeletal muscle). Muscle fibers with higher oxidative capacity contain large mitochondria tightly packed with cristae as well as small forms of mitochondria containing relatively few cristae. The intensive development of the mitochondrial apparatus in the post-activity period reflects the adaptive processes, which is intended to supply the increased energy requirements of muscle fibers with higher oxidative capacity. Muscle fibers with low oxidative capacity contain significantly less mitochondria than fibers with higher capacity. It is typical to type IIB fibers that after intensive muscle activity there are damaged myofibrils in a relatively small area, some myofibrils are twisted and lose the connection with the neighboring structures. It is still not fully known how skeletal muscles with different oxidative capacity respond to an increased functional activity and what differences exist in these fibers between oxidative capacity and function of myofibrils. The aim of the present short review was to compare structural-functional changes in mitochondrial and myofibrillar compartments of heart and skeletal muscle fibers with different oxidative capacity and the effect of increased functional activity on the interaction of these compartments.
基金support pro-vided by the National Science Foundation of China(No.52171274)the National Key Research and Development Project(No.2018YFC0810402)+2 种基金the Chongqing Elite In-novation and Entrepreneurship Demonstration Team(No.CQYC201903204)the Chongqing Special Post-doctoral Science Foundation(No.XM2019)the State Key Laboratory of Hydraulic Engineering Simulation and Safety(Tianjin University)(No.HESS-12).
文摘The structure of an air-floating caisson is suitable for the major structure of caisson-type artificial islands.Thus,it has been rapidly developed and widely used in the exploration and development of oil and gas fields in shallow sea and intertidal zones.Air-floating transportation technology is one of the key technologies employed in this structure.In this paper,the factors influencing the dynamic response characteristics of air-floating caisson with multi-compartments(AFCMC)were studied using model tests.The length and the height of each air-floating structure in the model were 1.0 and 0.1 m,respectively.In addition,the 1:100 models with 6,8,and 10 compartments under regular waves were tested in the wave flume,respectively.In the experiments,the respective water depths were set at 0.2,0.3,and 0.4 m,and the corresponding drafts were 0.05,0.06,and 0.07 m.Results show that with the increase of draft,the heave natural period increased and the maximum amplitude of the heave motion decreased.Meanwhile,the pitch motion decreased at 6 and 8 compartments and increased at 10 compartments.As the water depth increased,the maximum amplitude and amplitude change of heave and pitch motions first increased and then decreased.However,several amplitudes close to the maximum amplitude appeared in the measured period at shallower water depth,thereby indicating the vertical movements of the structure enhanced under shallow water.The increase in the number of compartments reduced the vertical movements under 6.0 m draft,but it increased the vertical movements under 5.0 and 7.0 m draft.Thus,increasing the number of compartments has a limited capacity to improve the motion performance of the structure.
基金This work was funded by the National Science Foundation of China(Grant No.U2033206)the Project of Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province(Grant No.MZ2022KF05,Grant No.MZ2022JB01)+3 种基金the project of Key Laboratory of Civil Aviation Emergency Science&Technology,CAAC(Grant No.NJ2022022,Grant No.NJ2023025)the project of Postgraduate Project of Civil Aviation Flight University of China(Grant No X2023-1)the project of the undergraduate innovation and entrepreneurship training program(Grant No 202210624024)the project of General Programs of the Civil Aviation Flight University of China(Grant No J2020-072).
文摘The implementation of early and accurate detection of aircraft cargo compartment fire is of great significance to ensure flight safety.The current airborne fire detection technology mostly relies on single-parameter smoke detection using infrared light.This often results in a high false alarm rate in complex air transportation envi-ronments.The traditional deep learning model struggles to effectively address the issue of long-term dependency in multivariate fire information.This paper proposes a multi-technology collaborative fire detection method based on an improved transformers model.Dual-wavelength optical sensors,flue gas analyzers,and other equipment are used to carry out multi-technology collaborative detection methods and characterize various feature dimensions of fire to improve detection accuracy.The improved Transformer model which integrates the self-attention mechanism and position encoding mechanism is applied to the problem of long-time series modeling of fire information from a global perspective,which effectively solves the problem of gradient disappearance and gradient explosion in traditional RNN(recurrent neural network)and CNN(convolutional neural network).Two different multi-head self-attention mechanisms are used to classify and model multivariate fire information,respectively,which solves the problem of confusing time series modeling and classification modeling in dealing with multivariate classification tasks by a single attention mechanism.Finally,the output results of the two models are fused through the gate mechanism.The research results show that,compared with the traditional single-feature detection technology,the multi-technology collaborative fire detection method can better capture fire information.Compared with the traditional deep learning model,the multivariate fire pre-diction model constructed by the improved Transformer can better detect fires,and the accuracy rate is 0.995.
基金This work is supported by the consulting research project of the major project of China National Railway Group Co.,Ltd.(No.K2020J003)the Chinese Academy of Engineering(No.2020-XY-79)。
文摘Contagious pathogens like COVID-19 transmitted via respiratory droplets spread effortlessly in the passenger compartments of transport,significantly jeopardizing passengers’safety when taking public transportation.To date,studies on the fundamental theories of airborne droplet transmission and the engineering application of decontamination techniques are insufficient for the prevention and control of pathogens transmitting in the compartments of passenger transport.It is essential to systematically investigate the control approaches to restrain pathogens from transmitting in passenger compartments.Herein,a theoretical framework for calculating the transmission of pathogens in a complex compartment environment was proposed,and experimental platforms that satisfy the Biosafety Level-2 Laboratory safety level for compartment environment simulations were built based on a set of real train cabins.On these bases,numerical investigations on the motion of pathogen-laden droplets were conducted,and decontamination techniques were examined experimentally.Thereby,control measures on the pathogen transmission and pathogen decontamination schemes were proposed.Moreover,highly efficient decontamination devices were developed,and coping strategies for epidemic emergencies were devised.The outcomes provide theoretical and technical support for developing the next generation of transportation and the prevention and control measures cooperatively considering regular and pandemic times.
基金the National Natural Science Foundation of China(No.52271287).
文摘This study investigates the wet towing characteristics of an integrated wellhead platform supported by a bucket foundation.These characteristics are crucial for optimizing offshore construction efficiency and enabling the development of small marginal oil fields.The wet towing behavior of the integrated wellhead platform was explored through a combination of physical experiments and numerical simulations.Physical experiments were conducted to validate the accuracy of the numerical simulations.Subsequently,numerical simulations were employed to determine the impacts of towing speed and wave direction on the towing process of the integrated wellhead platform.Finally,the impact of compartment failure due to bulkhead damage on towage stability was analyzed.Findings indicate that the wave and towing directions influence the motion of the platform at various degrees of freedom.The platform demonstrates optimal stability when towing against waves,particularly when the towing direction aligns at a specific angle relative to the wave direction.In addition,a controlled increase in towing speed within a specific range effectively mitigates roll and pitch motions,which enhances the overall tow stability of the platform.Notably,compartment failure has an adverse effect on the towing stability,particularly in aft compartments.Therefore,it requires careful consideration and attention in practical engineering scenarios.
基金supported by the National Natural Science Foundation of China(Grant No.U2341269)。
文摘The muzzle blast overpressure induces disturbances in the flow field inside the crew compartment(FFICC)of a truck-mounted howitzer during the artillery firing.This overpressure is the primary factor preventing personnel from firing artillery within the cab.To investigate the overpressure characteristics of the FFICC,a foreign trade equipment model was used as the research object,and a numerical model was established to analyze the propagation of muzzle blast from the muzzle to the interior of the crew compartment under extreme firing condition.For comparative verification,the muzzle blast experiment included overpressure data from both the flow field outside the crew compartment(FFOCC)and the FFICC,as well as the acceleration data of the crew compartment structure(Str-CC).The research findings demonstrate that the overpressure-time curves of the FFICC exhibit multi-peak characteristics,while the pressure wave shows no significant discontinuity.The enclosed nature of the cab hinders the dissipation of pressure wave energy within the FFICC,leading to sustained high-amplitude overpressure.The frameskin structure helps attenuate the impact of muzzle blast on the FFICC.Conversely,local high overpressure caused by the convex or concave features of the cab's exterior significantly amplifies the overpressure amplitude within the FFICC.
文摘Acute compartment syndrome(ACoS)involves a complex pathological process in which rising pressures within an enclosed muscle space ultimately result in anoxia and cell death.The most common etiologies include long bone fractures,crush injuries,and burns.[1,2]While rare,ACoS can also occur in atraumatic settings following periods of prolonged limb compression,such as intoxication.[3]Atraumatic ACoS is easily overlooked,resulting in diagnostic delay and poor patient outcomes.Prompt recognition and surgical management is essential to prevent critical sequelae.
文摘BACKGROUND Hypoganglionosis is a rare gastrointestinal acquired motility disorder that res-embles Hirschsprung’s disease and can manifest in the adult life.Abdominal compartment syndrome,a condition characterized by an increase in intra-abdo-minal pressure with physiological disturbance can be caused by severe massive fecal impaction.CASE SUMMARY A 33-year-old female presented to the emergency room with massive abdominal distension that rapidly progressed to abdominal compartment syndrome.The pa-tient was diagnosed with hypoganglionosis.Life-saving emergent proctoco-lectomy was performed to save the patient.CONCLUSION Abdominal compartment syndrome can develop secondary to excessive colonic distension.This extreme but rare situation must be addressed immediately.Hypo-ganglionosis is a potential cause of severe constipation that may present in adu-lthood.
文摘The spatial organization of the genome plays an important role in the regulation of gene expression. How- ever, the core structural features of animal genomes, such as topologically associated domains （TADs） and chromatin loops, are not prominent in the extremely compact Arabidopsis genome. In this study, we examine the chromatin architecture, as well as their DNA methylation, histone modifications, accessible chromatin, and gene expression, of maize, tomato, sorghum, foxtail millet, and rice with genome sizes ranging from 0.4 to 2.4 Gb. We found that these plant genomes can be divided into mammalian-like A/B compartments. At higher resolution, the chromosomes of these plants can be further partitioned to local AJB compartments that reflect their euchromatin, heterochromatin, and polycomb status. Chromatins in all these plants are organized into domains that are not conserved across species. They show similarity to the Drosophila compartment domains, and are clustered into active, polycomb, repressive, and interme- diate types based on their transcriptional activities and epigenetic signatures, with domain border overlaps with the local A/B compartment junctions. In the large maize and tomato genomes, we observed extensive chromatin loops. However, unlike the mammalian chromatin loops that are enriched at the TAD border, plant chromatin loops are often formed between gene islands outside the repressive domains and are closely associated with active compartments. Our study indicates that plants have complex and unique 3D chromatin architectures, which require further study to elucidate their biological functions.
基金Supported by grants from the Research Grants Council of Hong Kong(CUHK4156/01M,CUHK4260/02M,CUHK4307/03M,and CUHK4580/05M)National Science Foundation of China (30529001)+1 种基金CUHK Scheme C,UGCAoE(B-07/99)Germany/HK Joint Research Scheme to L.Jiang.
文摘Prevacuolar compartments （PVCs） and endosomal compartments are membrane-bound organelles mediating protein traffic to vacuoles in the secretory and endocytic pathways of plant cells. Over the years, great progress has been made towards our understanding in these two compartments in plant cells. In this review, we will summarize our contributions toward the identification and characterization of plant prevacuolar and endosomal compartments. Our studies will serve as important steps in future molecular characterization of PVC biogenesis and PVC-mediated protein traffickinq in plant cells.
基金supported by the National Key R&D Program of China(Grant No.2018YFC1003500 awarded to BW)the National Natural Science Foundation of China(Grant No.31771435 awarded to BW)
文摘In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these architectures remain incompletely understood. Here, we investigated the functional roles of H3 lysine 9 dimethylation(H3 K9 me2), one of the abundant histone modifications, in three-dimensional(3 D)genome organization. Unlike in mouse embryonic stem cells, inhibition of methyltransferases G9 a and GLP in differentiated cells eliminated H3 K9 me2 predominantly at A-type(active) genomic compartments, and the level of residual H3 K9 me2 modifications was strongly associated with B-type(inactive) genomic compartments. Furthermore, chemical inhibition of G9 a/GLP in mouse hepatocytes led to decreased chromatin-nuclear lamina interactions mainly at G9 a/GLP-sensitive regions, increased degree of genomic compartmentalization, and up-regulation of hundreds of genes that were associated with alterations of the 3 D chromatin. Collectively, our data demonstrated essential roles of H3 K9 me2 in 3 D genome organization.
基金supported by the National Natural Science Foundation of China(Grant Nos.82173620 to Yang Zhao and 82041024 to Feng Chen)partially supported by the Bill&Melinda Gates Foundation(Grant No.INV-006371 to Feng Chen)Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Deterministic compartment models(CMs)and stochastic models,including stochastic CMs and agent-based models,are widely utilized in epidemic modeling.However,the relationship between CMs and their corresponding stochastic models is not well understood.The present study aimed to address this gap by conducting a comparative study using the susceptible,exposed,infectious,and recovered(SEIR)model and its extended CMs from the coronavirus disease 2019 modeling literature.We demonstrated the equivalence of the numerical solution of CMs using the Euler scheme and their stochastic counterparts through theoretical analysis and simulations.Based on this equivalence,we proposed an efficient model calibration method that could replicate the exact solution of CMs in the corresponding stochastic models through parameter adjustment.The advancement in calibration techniques enhanced the accuracy of stochastic modeling in capturing the dynamics of epidemics.However,it should be noted that discrete-time stochastic models cannot perfectly reproduce the exact solution of continuous-time CMs.Additionally,we proposed a new stochastic compartment and agent mixed model as an alternative to agent-based models for large-scale population simulations with a limited number of agents.This model offered a balance between computational efficiency and accuracy.The results of this research contributed to the comparison and unification of deterministic CMs and stochastic models in epidemic modeling.Furthermore,the results had implications for the development of hybrid models that integrated the strengths of both frameworks.Overall,the present study has provided valuable epidemic modeling techniques and their practical applications for understanding and controlling the spread of infectious diseases.
文摘BACKGROUND Unicompartmental knee arthroplasty(UKA)and high tibial osteotomy(HTO)are well-established operative interventions in the treatment of knee osteoarthritis.However,which intervention is more beneficial to patients with knee osteoarthritis remains unknown and a topic of much debate.Simultaneously,there is a paucity of research assessing the relationship between radiographic parameters of knee joint alignment and patient-reported clinical outcomes,preoperatively and following HTO or UKA.AIM To compare UKAs and HTOs as interventions for medial-compartment knee osteoarthritis:Examining differences in clinical outcome and investigating the relationship of joint alignment with respect to this.METHODS This longitudinal observational study assessed a total of 42 patients that had undergone UKA(n=23)and HTO(n=19)to treat medial compartment knee osteoarthritis.Patient-reported outcome measures(PROMs)were collected to evaluate clinical outcome.These included two disease-specific(Knee Injury and Osteoarthritis Outcome Score,Oxford Knee Score)and two generic(EQ-5D-5L,Short Form-12)PROMs.The radiographic parameters of knee alignment assessed were the:Hip-knee-ankle angle,mechanical axis deviation and angle of Mikulicz line.RESULTS Statistical analyses demonstrated significant(P<0.001),preoperative to postoperative,improvements in the PROM scores of both groups.There were,however,no significant inter-group differences in the postoperative PROM scores of the UKA and HTO group.Several significant correlations associated a more distolaterally angled Mikulicz line with worse knee function and overall health preoperatively(P<0.05).Postoperatively,two clusters of significant correlations were observed between the disease-specific PROM scores and knee joint alignment parameters(hip-knee-ankle angle,mechanical axis deviation)within the HTO group;yet no such associations were observed within the UKA group.CONCLUSION UKAs and HTOs are both efficacious operations that provide a comparable degree of clinical benefit to patients with medial compartment knee osteoarthritis.Clinical outcome has a limited association with radiographic parameters of knee joint alignment postoperatively;however,a more distolaterally angled Mikulicz line appears associated with worse knee function/health-related quality of life preoperatively.
文摘We suggest that during severe acute pancreatitis(SAP)with intra-abdominal hypertension,practitioners should consider decompressive laparotomy,even with intra-abdominal pressure(IAP)below 25 mmHg.Indeed,in this setting,non-occlusive mesenteric ischemia(NOMI)may occur even with IAP below this cutoff and lead to transmural necrosis if abdominal perfusion pressure is not promptly restored.We report our experience of 18 critically ill patients with SAP having undergone decompressive laparotomy of which one third had NOMI while IAP was mostly below 25 mmHg.
基金supported by the National Key R&D Program of China (2022YFF1000100 to Long Jin and 2023YFD1300012 to Long Jin)the Sichuan Science and Technology Program (2022JDJQ0054 to Long Jin and 2021YFYZ0009 to Mingzhou Li)the National Natural Science Foundation of China (32225046 to Mingzhou Li)。
文摘Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake. As a result, goose offers an excellent model for studying obesity, metabolic disorders, and liver diseases in mammals. Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged, the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.Results In this study, geese exhibited more pronounced changes in the liver index and triglyceride(TG) content following the consumption of the high-fat diet(HFD) than mice without significant signs of inflammation. Additionally, we performed comprehensive analyses on 10 goose liver tissues(5 HFD, 5 normal), including generating highresolution maps of chromatin architecture, conducting whole-genome gene expression profiling, and identifying H3K27ac peaks in the livers of geese and mice subjected to the HFD. Our results unveiled a multiscale restructuring of chromatin architecture, encompassing Compartment A/B, topologically associated domains, and interactions between promoters and enhancers. The dynamism of the three-dimensional genome architecture, prompted by the HFD, assumed a pivotal role in the transcriptional regulation of crucial genes. Furthermore, we identified genes that regulate chromatin conformation changes, contributing to the metabolic adaptation process of lipid deposition and hepatic fat changes in geese in response to excessive energy intake. Moreover, we conducted a cross-species analysis comparing geese and mice exposed to the HFD, revealing unique characteristics specific to the goose liver compared to a mouse. These chromatin conformation changes help elucidate the observed characteristics of fat deposition and hepatic fat regulation in geese under conditions of excessive energy intake.Conclusions We examined the dynamic modifications in three-dimensional chromatin architecture and gene expression induced by an HFD in goose liver tissues. We conducted a cross-species analysis comparing that of mice. Our results contribute significant insights into the chromatin architecture of goose liver tissues, offering a novel perspective for investigating mammal liver diseases.
