[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavo...[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.展开更多
[Objective] The aim was to explore the effects of Ca2＋ on the negative phototropism of Chorophytum comosum root.[Method] C.comosum were cultivated in different concentration of CaCl2 solution with unilateral light il...[Objective] The aim was to explore the effects of Ca2＋ on the negative phototropism of Chorophytum comosum root.[Method] C.comosum were cultivated in different concentration of CaCl2 solution with unilateral light illuminated.[Rusult] The Ca2＋ in nutrient solution showed significant effect on the growth and tropic reaction of C.comosum;low concentration of Ca2＋ promoted root growth,while high concentrations of Ca2＋ inhibited the root growth.When the concentration of Ca2＋ was 0.4 mmol/L,the phenomenon of the negative phototropism and the root growth were the most obvious.[Conclusion] The root negative phototropism could be concluded to the comprehensive effect of the endogenous and exogenous Ca2＋.展开更多
Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against diff...Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.展开更多
Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutr...Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutral red （ Treatment T3 ） on the biomass, root-shoot ratio, leaf color indices, plant carbon and nitrogen nutrition were studied. The results showed that the biomass of Treatment T3 was significantly greater than that of treatments T1 and CK. The root-shoot ratio decreased significantly in treatments T1, T2 and T3 , and the decrease in T3 was most obvious. In all the three treatments with coloring agent, a ^＊ , b ^＊ and L ^＊ values were increased gradually, C value were decreased, H0 and CIRG were increased, and the leaves were pink. In addition, the contents of chlorophyll a, chlorophyll b, chlorophyll a ＋ b and carotenoid were significantly decreased. The contents of soluble sugar and starch were also decreased, and the decrease in Treatment T2 was most significant. The contents of soluble protein and total nitrogen were increased, and the increase was most dramatic in Treatment T3. The carbon to nitrogen ratio was decreased. The results proved that staining can improve the ornamental value of indoor plants, despite its effects on plant carbon and nitrogen nutrition of C. comosum vat. variegatum, dyeing.展开更多
The aerial roots of Chlorophytum comosum were grown hydroponically,allowing us to study the performance and mechanism of negative phototropism. The results of this study were as follows. All the adventitious roots and...The aerial roots of Chlorophytum comosum were grown hydroponically,allowing us to study the performance and mechanism of negative phototropism. The results of this study were as follows. All the adventitious roots and their branch roots bent away from light with a maximum curvature of approximately 88.5°. Blue-violet light prominently induced negative phototropism while red light had no effect. The root cap was the site of photo perception. Roots with shaded or divested root caps exposed to unilateral light showed no negative phototropism,but resumed their original characteristics when the shade was removed or when new root caps grew. The curvature increased when the light intensity ranged 0–110 μmol·m^(-2)·s^(-1). The negative phototropism curvature could be promoted by exogenous CaCl_2 but was inhibited by exogenous LaCl_3; exogenous CaCl_2 could reduce the inhibitory effect of LaCl_3. Unilateral light induced the horizontal transport of IAA from the irradiated side to the shaded side,resulting in an unequal distribution of IAA in both the sides,leading to negative phototropism. The horizontal transport of IAA was promoted by exogenous Ca^(2+) but inhibited by exogenous La^(3+).展开更多
Environmentally friendly green synthesis of nanomaterial has a very significant part in nanotechnology.In the present research,the synthesis of silver nanoparticles(AgNPs)was established by treating silver ions with t...Environmentally friendly green synthesis of nanomaterial has a very significant part in nanotechnology.In the present research,the synthesis of silver nanoparticles(AgNPs)was established by treating silver ions with the aqueous extract of Calligonum comosum green shoots at room temperature.AgNPs formation was firstly detected by the colour change of mixed extract(plant extract and AgNO3).Further characterization was done by ultraviolet,(UV)-Vis spectrophotometer,transmission electron microscopy(TEM),scanning electron microscopy(SEM),zeta potential and fourier transform infrared spectroscopy(FTIR).The peak values for UV-VIS-spectroscopy were in the range of 440 nm,TEM micrograph showed spherical shape for the particles and zeta potential showed formation of negative charged nanoparticles with an average size of about 105.8 nm.1635.41 and 3249.83 cm^(-1) are the peaks detected from the FTIR analysis.In this study,biosynthesized silver nanoparticles mediated by C.comosum were tested for their antimycotic activity using a well diffusion method against fungal species;Aspergillus flavus,Penicillium sp,Fusarium oxysporum.Our findings indicated that biosynthesized AgNPs showed an efficient antimycotic activity against tested species.The antimycotic action of AgNPs varied according to different fungal species.Results confirmed the ability of C.comosum green shoot extract to act as an reducing and stabilizing agent during the synthesis of AgNPs.展开更多
基金Supported by Youth Fund Project of Zhaoqing University(QZ202235)Zhaoqing Science and Technology Plan Project(2022040311011).
文摘[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.
基金Supported by the Natural Science Foundation of Hubei Province (2006ABA186)the Youth Project of the Provincial Education Department of Hubei Province(Q200727004),the Natural Science Foundation for Youth and Middleages of Provincial Education Department(Q200727003)~~
文摘[Objective] The aim was to explore the effects of Ca2＋ on the negative phototropism of Chorophytum comosum root.[Method] C.comosum were cultivated in different concentration of CaCl2 solution with unilateral light illuminated.[Rusult] The Ca2＋ in nutrient solution showed significant effect on the growth and tropic reaction of C.comosum;low concentration of Ca2＋ promoted root growth,while high concentrations of Ca2＋ inhibited the root growth.When the concentration of Ca2＋ was 0.4 mmol/L,the phenomenon of the negative phototropism and the root growth were the most obvious.[Conclusion] The root negative phototropism could be concluded to the comprehensive effect of the endogenous and exogenous Ca2＋.
文摘Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.
文摘Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutral red （ Treatment T3 ） on the biomass, root-shoot ratio, leaf color indices, plant carbon and nitrogen nutrition were studied. The results showed that the biomass of Treatment T3 was significantly greater than that of treatments T1 and CK. The root-shoot ratio decreased significantly in treatments T1, T2 and T3 , and the decrease in T3 was most obvious. In all the three treatments with coloring agent, a ^＊ , b ^＊ and L ^＊ values were increased gradually, C value were decreased, H0 and CIRG were increased, and the leaves were pink. In addition, the contents of chlorophyll a, chlorophyll b, chlorophyll a ＋ b and carotenoid were significantly decreased. The contents of soluble sugar and starch were also decreased, and the decrease in Treatment T2 was most significant. The contents of soluble protein and total nitrogen were increased, and the increase was most dramatic in Treatment T3. The carbon to nitrogen ratio was decreased. The results proved that staining can improve the ornamental value of indoor plants, despite its effects on plant carbon and nitrogen nutrition of C. comosum vat. variegatum, dyeing.
基金supported by the Postdoctoral Fund of Jiangsu Province(Grant No.1402115C)Open Fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization of Huanggang Normal University,China (Grant No.2013000503)+2 种基金Industry University Cooperation Fund of Huanggang Normal University,China (Grant No.2012025703)New Forestry Major Project of Jiangsu Province(Grant No.lysx201305)National Natural Science Foundation of China(Grant No.31270228)
文摘The aerial roots of Chlorophytum comosum were grown hydroponically,allowing us to study the performance and mechanism of negative phototropism. The results of this study were as follows. All the adventitious roots and their branch roots bent away from light with a maximum curvature of approximately 88.5°. Blue-violet light prominently induced negative phototropism while red light had no effect. The root cap was the site of photo perception. Roots with shaded or divested root caps exposed to unilateral light showed no negative phototropism,but resumed their original characteristics when the shade was removed or when new root caps grew. The curvature increased when the light intensity ranged 0–110 μmol·m^(-2)·s^(-1). The negative phototropism curvature could be promoted by exogenous CaCl_2 but was inhibited by exogenous LaCl_3; exogenous CaCl_2 could reduce the inhibitory effect of LaCl_3. Unilateral light induced the horizontal transport of IAA from the irradiated side to the shaded side,resulting in an unequal distribution of IAA in both the sides,leading to negative phototropism. The horizontal transport of IAA was promoted by exogenous Ca^(2+) but inhibited by exogenous La^(3+).
文摘Environmentally friendly green synthesis of nanomaterial has a very significant part in nanotechnology.In the present research,the synthesis of silver nanoparticles(AgNPs)was established by treating silver ions with the aqueous extract of Calligonum comosum green shoots at room temperature.AgNPs formation was firstly detected by the colour change of mixed extract(plant extract and AgNO3).Further characterization was done by ultraviolet,(UV)-Vis spectrophotometer,transmission electron microscopy(TEM),scanning electron microscopy(SEM),zeta potential and fourier transform infrared spectroscopy(FTIR).The peak values for UV-VIS-spectroscopy were in the range of 440 nm,TEM micrograph showed spherical shape for the particles and zeta potential showed formation of negative charged nanoparticles with an average size of about 105.8 nm.1635.41 and 3249.83 cm^(-1) are the peaks detected from the FTIR analysis.In this study,biosynthesized silver nanoparticles mediated by C.comosum were tested for their antimycotic activity using a well diffusion method against fungal species;Aspergillus flavus,Penicillium sp,Fusarium oxysporum.Our findings indicated that biosynthesized AgNPs showed an efficient antimycotic activity against tested species.The antimycotic action of AgNPs varied according to different fungal species.Results confirmed the ability of C.comosum green shoot extract to act as an reducing and stabilizing agent during the synthesis of AgNPs.
