This paper explores ambient affiliation within the ASMR community on YouT ube,investigating how values are communed around in YouT ube comments.This community consists of viewers who watch videos designed to elicit an...This paper explores ambient affiliation within the ASMR community on YouT ube,investigating how values are communed around in YouT ube comments.This community consists of viewers who watch videos designed to elicit an Autonomous Sensory Meridian Response(ASMR),that is,a pleasurable sensory reaction to visual or aural stimuli.The paper focuses on the linguistic resources involved in orienting the ambient audience to the values shared,as couplings of ATTITUDE and IDEATION,in the comments feed.Because most comments do not receive any reply,rather than dialogic affiliation,a framework for analysing communing affiliation,based on Zappavigna and Martin’s(2018)approach to Twitter data,is applied.Three systems of meaning are considered:convoking(mustering community around a coupling),finessing(dialogically positioning a coupling),and promoting(emphasising a coupling).The paper develops these systems in terms of delicacy.The attitudinal component of the couplings is analysed using the Appraisal framework(Martin and White 2005).The dataset explored is the comments feed of the most viewed video on the Gentle Whispering ASMR YouT ube channel,the most popular ASMR channel at the time of writing.展开更多
Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and ...Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.展开更多
This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses...This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity.Moreover,the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention.In addition,NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae,g_Alloprevotella,and f_Eubacterium_coprostanoligenes_group reduced by Pb.Also,NCVP regulated the diversity and abundance of gut mycobiota affected by Pb.Specifically,Pb decreased the proportion of pathogenic species(g_Fusarium,p_Basidiomycota,g_Alternaria,g_Aspergillus,and g_Candida)while NCVP increased the abundance of probiotics species(g_Kazachstania and p_Ascomycota).Furthermore,the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites,including porphobilinogen,cromakalim,salidroside,and trichostatin A,which has significant associations with specific gut bacteriobiota or mycobiota.These altered metabolites are involved in primary bile acid biosynthesis,metabolism of xenobiotics by cytochrome P450,lysine degradation,and other metabolic pathways.Overall,our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity,possibly by regulating gut bacteriome,mycobiome and metabolome.展开更多
The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristic...The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.展开更多
为了研究不同地区地木耳(Nostoc commune Vauch.)多糖的结构与体外抗氧化活性,用苯酚-硫酸法测定地木耳多糖含量,用傅里叶变换红外光谱法分析多糖结构,用清除DPPH自由基法、清除超氧阴离子法、清除羟基自由基法测定不同地区地木耳多糖...为了研究不同地区地木耳(Nostoc commune Vauch.)多糖的结构与体外抗氧化活性,用苯酚-硫酸法测定地木耳多糖含量,用傅里叶变换红外光谱法分析多糖结构,用清除DPPH自由基法、清除超氧阴离子法、清除羟基自由基法测定不同地区地木耳多糖体外抗氧化活性。结果表明,不同地区地木耳多糖含量有差异,蓬溪和名山地木耳多糖含量高于南部地木耳多糖,含量分别为(18.29%±0.21%)和(17.18%±0.17%)。不同地木耳多糖红外光谱峰形、位置相似,但峰强有差异。地木耳多糖对DPPH自由基、超氧阴离子、羟基自由基具有较强的清除能力;地木耳多糖抗氧化活性比地木耳提取液强,但比维生素C弱;随着浓度增加,地木耳多糖抗氧化活力增强;蓬溪和名山地木耳多糖抗氧化活性强于南部地木耳多糖抗氧化活性。由此说明,不同地区地木耳多糖含量与其组成有差异,不同地区地木耳多糖抗氧化活性差异大。展开更多
采用醇提取法对地木耳(Nostoc commune Vauch)活性成分进行提取,以地木耳乙醇提取物与大肠杆菌作用前后细菌培养液的电导率、可溶性总糖和总蛋白质浓度变化,探究地木耳乙醇提取物对大肠杆菌的抑菌机理,对配制出的化妆品,进行了稳定性、...采用醇提取法对地木耳(Nostoc commune Vauch)活性成分进行提取,以地木耳乙醇提取物与大肠杆菌作用前后细菌培养液的电导率、可溶性总糖和总蛋白质浓度变化,探究地木耳乙醇提取物对大肠杆菌的抑菌机理,对配制出的化妆品,进行了稳定性、安全性、保湿、美白等测试。结果表明,在经过地木耳乙醇提取物处理后,大肠杆菌培养液的电导率、可溶性总糖和总蛋白质浓度均有不同程度的升高。配制的化妆品安全性、稳定性良好,且具一定的美白、保湿性。地木耳乙醇提取物可使大肠杆菌细胞膜发生破损,所开发的化妆品细腻温和、质地稳定均一、安全无毒副作用,并具有美白、保湿等功效。展开更多
采用正交试验分析了不同环境条件对念珠藻地木耳(Nostoc commune Vauch)生物量和多糖分泌的影响。结果表明,在适宜的温度、光强、盐度和UV-B处理对地木耳的生长、胞外多糖分泌及其抗氧化能力有明显的促进作用。培养条件为光照强度100μm...采用正交试验分析了不同环境条件对念珠藻地木耳(Nostoc commune Vauch)生物量和多糖分泌的影响。结果表明,在适宜的温度、光强、盐度和UV-B处理对地木耳的生长、胞外多糖分泌及其抗氧化能力有明显的促进作用。培养条件为光照强度100μmol/(m2·s)、温度30℃、Na Cl浓度0 mmol/L和UVB辐射强度10 k J/(m2·d)时,地木耳有最大的相对生长率;光照强度200μmol/(m2·s)、温度35℃、Na Cl浓度40 mmol/L和UV-B辐射强度20 k J/(m2·d)时,胞外多糖的分泌强度最高,温度和UV-B处理分别对相对生长率和胞外多糖含量的提高有显著效果。展开更多
Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosil...The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography(RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance(NMR). The effects of these compounds on voltage-gated sodium(NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant(TTX-r) and tetrodotoxin-sensitive(TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.展开更多
The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-di...The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)radical scavenging activities,as well as ferric ion reducing antioxidant power(FRAP).The total phenolic content(TPC)and total flavonoid content(TFC)were also determined.Additionally,the changes caused by fermentation were characterized by proton nuclear magnetic resonance spectroscopy(1H NMR).The results showed that P.fortuneana fruits fermented with Rhizopus oryzae and Penicillium commune exhibited stronger antioxidant activity compared with the non-fermented material.Correlation analysis indicated that the increased activity might be ascribed to the improvement of TPC.Chemical characterization of 1H NMR implied that the increased TPC and antioxidant activity might be attributed to some of the phenolic and flavonoid glycosides hydrolyzed to new components during fermentation.The present study was the first report of the fermentation of P.fortuneana fruit with pure fungal strains and suggested that fermentation of P.fortuneana fruits with R.oryzae and P.commune was an effective alternative approach to increase antioxidant,and P.fortuneana fruit fermented by R.oryzae and P.commune might be the new alternative of natural antioxidants.展开更多
The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flage...The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flagelliforme cells can be stimulated by La(NO3)(3) at the concentrations from 1 to 200 mg.L-1, and among them the treatment of 100 mg.L-1 La(NO3)(3) has the largest A(670) and dry weight which increased by 64.9% and 61.1%, respectively as compared with the control, and La(NO3)(3) can also influence the contents of some kinds of amino acids in Nostoc commune flagelliforme cells. The contents of aspartic acid and glutamic acid decreased by 59.9% and 57.9%, respectively, and that of proline increased by 24.5% with the treatment of 200 mg.L-1 La(NO3)(3).展开更多
Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in...Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in general and in the province of Bandundu, in particular by assessing the selenium content and the antioxidant capacity of wild edible mushrooms. Methanolic extracts from selected mushrooms were characterized for their chemical fingerprint by TLC and their in vitro antioxidant activities using ABTS, DPPH assays. Phytochemical screening revealed the presence of alkaloids, free amines, steroids and terpenes in all species. However, Auricularia delicata and Pleurotus tuberregium contain flavonoids. All extracts displayed a good radical-scavenging activity at the concentration range 1 - 300 μg/mL in the following order: Auricularia delicata > Lentinus cf cladopus > Pleurotus tuberregium > Marasmius buzungolo > Schizophillum commune. Studied mushrooms showed the interest quantity of selenium and L. cf cladodus had the highest concentration. Moderate selenium content of these wild mushrooms associated with their important antioxidant activities could provide health benefits to Bandundu’s populations in protecting against oxidative damage under different conditions including konzo.展开更多
Morphological characteristics and proximate chemical analysis of E. binata showed it as a suitable raw material for writing and printing grade paper. Maximum pulp yield (43.58%) of kappa number 17.38 with 0.9% screeni...Morphological characteristics and proximate chemical analysis of E. binata showed it as a suitable raw material for writing and printing grade paper. Maximum pulp yield (43.58%) of kappa number 17.38 with 0.9% screening rejects was obtained at 12% of active alkali (as Na2O) pulping temperature 130°C, cooking time 120 min and solid to liquor ratio 1:4. During bio-soda pulping of E. binata, pulp yield decreased slightly while pulp brightness, tensile index, burst index and double fold numbers were improved by 4.1%, 24.94%, 14.03% and 48.45% respectively compared to soda pulping. During ethanol-soda pulping of E. binata, pulp yield, pulp brightness, tensile index, burst index and double fold numbers were improved by 3.9%, 6.6%, 32.18%, 35.40% and 77.31% respectively compared to soda pulping.展开更多
Plant growth and productivity are negatively affected by soil salinity.This study investigated the effects of the rhizosphere-promoting bacterium,Bacillus paramycoides JYZ-SD5,and the ectomycorrhizal fungus,Schizophyl...Plant growth and productivity are negatively affected by soil salinity.This study investigated the effects of the rhizosphere-promoting bacterium,Bacillus paramycoides JYZ-SD5,and the ectomycorrhizal fungus,Schizophyllum commune Be,on the growth of Metasequoia glyptostroboides under salt stress.Changes in biomass,root growth,root ion distribution and in vivo enzyme activities were determined under different treatments(Be,JYZ-SD5,and Be+JYZ-SD5).The results show that all inoculations increased chlorophyll content,shoot length and root diameter with or without salt stress,and the effect of Be+JYZ-SD5was the strongest.JYZ-SD5 and Be+JYZ-SD5 treatments significantly increased root length,surface area,bifurcation number,tip number,main root length and diameter under salt stress.Normal chloroplast structures developed under both single and double inoculations.Relative to the control,root activities of M.glyptostroboides in the Be,JYZSD5,and Be+JYZ-SD5 treatments increased by 31.3%,17.2%,and 33.7%.All treatments increased the activities of superoxide dismutase(SOD),peroxidase(POD),Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase.The strongest effect was by Be+JYZ-SD5.Analysis of root ion distribution showed that,under salt stress,Na^(+)and K^(+)decreased and were concentrated in the epidermis or cortex.Na/K ratios also decreased.The Be+JYZ-SD5 treatment increased betaine by 130.3%and 97.9%under 50 mM and 100 mM salt stress,respectively.Together,these changes result in the activation of physiological and biochemical processes involved in the mitigation of salinity-induced stress in M.glyptostroboides.展开更多
From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune w...From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune was characterized by rounded scutella, which was varied from two annules anterior to the anus and five annules posterior. The lateral field was not areolated at the level of scutella. S. magniphasma was characterized by varying scutellum, about 7.0 μm in diameter, in position from four annules posterior to four annules anterior to the anus, and areolated at the level of the scutellum.展开更多
文摘This paper explores ambient affiliation within the ASMR community on YouT ube,investigating how values are communed around in YouT ube comments.This community consists of viewers who watch videos designed to elicit an Autonomous Sensory Meridian Response(ASMR),that is,a pleasurable sensory reaction to visual or aural stimuli.The paper focuses on the linguistic resources involved in orienting the ambient audience to the values shared,as couplings of ATTITUDE and IDEATION,in the comments feed.Because most comments do not receive any reply,rather than dialogic affiliation,a framework for analysing communing affiliation,based on Zappavigna and Martin’s(2018)approach to Twitter data,is applied.Three systems of meaning are considered:convoking(mustering community around a coupling),finessing(dialogically positioning a coupling),and promoting(emphasising a coupling).The paper develops these systems in terms of delicacy.The attitudinal component of the couplings is analysed using the Appraisal framework(Martin and White 2005).The dataset explored is the comments feed of the most viewed video on the Gentle Whispering ASMR YouT ube channel,the most popular ASMR channel at the time of writing.
基金supported by the State Administration for Market Regulation Science and Technology Plan Project under Grant 2022MK192.
文摘Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.
基金supported by the Program of the National Natural Science Foundation of China(31872519)General Project of Jilin Provincial Department of Science and Technology(20230101247JC)the Open Research Fund of Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education.(KF202002).
文摘This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity.Moreover,the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention.In addition,NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae,g_Alloprevotella,and f_Eubacterium_coprostanoligenes_group reduced by Pb.Also,NCVP regulated the diversity and abundance of gut mycobiota affected by Pb.Specifically,Pb decreased the proportion of pathogenic species(g_Fusarium,p_Basidiomycota,g_Alternaria,g_Aspergillus,and g_Candida)while NCVP increased the abundance of probiotics species(g_Kazachstania and p_Ascomycota).Furthermore,the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites,including porphobilinogen,cromakalim,salidroside,and trichostatin A,which has significant associations with specific gut bacteriobiota or mycobiota.These altered metabolites are involved in primary bile acid biosynthesis,metabolism of xenobiotics by cytochrome P450,lysine degradation,and other metabolic pathways.Overall,our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity,possibly by regulating gut bacteriome,mycobiome and metabolome.
文摘The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.
文摘为了研究不同地区地木耳(Nostoc commune Vauch.)多糖的结构与体外抗氧化活性,用苯酚-硫酸法测定地木耳多糖含量,用傅里叶变换红外光谱法分析多糖结构,用清除DPPH自由基法、清除超氧阴离子法、清除羟基自由基法测定不同地区地木耳多糖体外抗氧化活性。结果表明,不同地区地木耳多糖含量有差异,蓬溪和名山地木耳多糖含量高于南部地木耳多糖,含量分别为(18.29%±0.21%)和(17.18%±0.17%)。不同地木耳多糖红外光谱峰形、位置相似,但峰强有差异。地木耳多糖对DPPH自由基、超氧阴离子、羟基自由基具有较强的清除能力;地木耳多糖抗氧化活性比地木耳提取液强,但比维生素C弱;随着浓度增加,地木耳多糖抗氧化活力增强;蓬溪和名山地木耳多糖抗氧化活性强于南部地木耳多糖抗氧化活性。由此说明,不同地区地木耳多糖含量与其组成有差异,不同地区地木耳多糖抗氧化活性差异大。
文摘采用醇提取法对地木耳(Nostoc commune Vauch)活性成分进行提取,以地木耳乙醇提取物与大肠杆菌作用前后细菌培养液的电导率、可溶性总糖和总蛋白质浓度变化,探究地木耳乙醇提取物对大肠杆菌的抑菌机理,对配制出的化妆品,进行了稳定性、安全性、保湿、美白等测试。结果表明,在经过地木耳乙醇提取物处理后,大肠杆菌培养液的电导率、可溶性总糖和总蛋白质浓度均有不同程度的升高。配制的化妆品安全性、稳定性良好,且具一定的美白、保湿性。地木耳乙醇提取物可使大肠杆菌细胞膜发生破损,所开发的化妆品细腻温和、质地稳定均一、安全无毒副作用,并具有美白、保湿等功效。
文摘采用正交试验分析了不同环境条件对念珠藻地木耳(Nostoc commune Vauch)生物量和多糖分泌的影响。结果表明,在适宜的温度、光强、盐度和UV-B处理对地木耳的生长、胞外多糖分泌及其抗氧化能力有明显的促进作用。培养条件为光照强度100μmol/(m2·s)、温度30℃、Na Cl浓度0 mmol/L和UVB辐射强度10 k J/(m2·d)时,地木耳有最大的相对生长率;光照强度200μmol/(m2·s)、温度35℃、Na Cl浓度40 mmol/L和UV-B辐射强度20 k J/(m2·d)时,胞外多糖的分泌强度最高,温度和UV-B处理分别对相对生长率和胞外多糖含量的提高有显著效果。
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
基金partly supported by Ministry of Science and Technology of China(No.2013CB911300)National Natural Science Foundation of China(Nos.81225024,31025025,31260208,and U1132601)
文摘The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography(RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance(NMR). The effects of these compounds on voltage-gated sodium(NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant(TTX-r) and tetrodotoxin-sensitive(TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.
基金Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities(Grant No.2019FH001-109)Undergraduates Innovative Experiment Project from MOE(Grant Nos.202010684016,202110684004)。
文摘The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)radical scavenging activities,as well as ferric ion reducing antioxidant power(FRAP).The total phenolic content(TPC)and total flavonoid content(TFC)were also determined.Additionally,the changes caused by fermentation were characterized by proton nuclear magnetic resonance spectroscopy(1H NMR).The results showed that P.fortuneana fruits fermented with Rhizopus oryzae and Penicillium commune exhibited stronger antioxidant activity compared with the non-fermented material.Correlation analysis indicated that the increased activity might be ascribed to the improvement of TPC.Chemical characterization of 1H NMR implied that the increased TPC and antioxidant activity might be attributed to some of the phenolic and flavonoid glycosides hydrolyzed to new components during fermentation.The present study was the first report of the fermentation of P.fortuneana fruit with pure fungal strains and suggested that fermentation of P.fortuneana fruits with R.oryzae and P.commune was an effective alternative approach to increase antioxidant,and P.fortuneana fruit fermented by R.oryzae and P.commune might be the new alternative of natural antioxidants.
文摘The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flagelliforme cells can be stimulated by La(NO3)(3) at the concentrations from 1 to 200 mg.L-1, and among them the treatment of 100 mg.L-1 La(NO3)(3) has the largest A(670) and dry weight which increased by 64.9% and 61.1%, respectively as compared with the control, and La(NO3)(3) can also influence the contents of some kinds of amino acids in Nostoc commune flagelliforme cells. The contents of aspartic acid and glutamic acid decreased by 59.9% and 57.9%, respectively, and that of proline increased by 24.5% with the treatment of 200 mg.L-1 La(NO3)(3).
文摘Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in general and in the province of Bandundu, in particular by assessing the selenium content and the antioxidant capacity of wild edible mushrooms. Methanolic extracts from selected mushrooms were characterized for their chemical fingerprint by TLC and their in vitro antioxidant activities using ABTS, DPPH assays. Phytochemical screening revealed the presence of alkaloids, free amines, steroids and terpenes in all species. However, Auricularia delicata and Pleurotus tuberregium contain flavonoids. All extracts displayed a good radical-scavenging activity at the concentration range 1 - 300 μg/mL in the following order: Auricularia delicata > Lentinus cf cladopus > Pleurotus tuberregium > Marasmius buzungolo > Schizophillum commune. Studied mushrooms showed the interest quantity of selenium and L. cf cladodus had the highest concentration. Moderate selenium content of these wild mushrooms associated with their important antioxidant activities could provide health benefits to Bandundu’s populations in protecting against oxidative damage under different conditions including konzo.
文摘Morphological characteristics and proximate chemical analysis of E. binata showed it as a suitable raw material for writing and printing grade paper. Maximum pulp yield (43.58%) of kappa number 17.38 with 0.9% screening rejects was obtained at 12% of active alkali (as Na2O) pulping temperature 130°C, cooking time 120 min and solid to liquor ratio 1:4. During bio-soda pulping of E. binata, pulp yield decreased slightly while pulp brightness, tensile index, burst index and double fold numbers were improved by 4.1%, 24.94%, 14.03% and 48.45% respectively compared to soda pulping. During ethanol-soda pulping of E. binata, pulp yield, pulp brightness, tensile index, burst index and double fold numbers were improved by 3.9%, 6.6%, 32.18%, 35.40% and 77.31% respectively compared to soda pulping.
基金supported by the National Key Research and Development Program of China (2017YFD0600104)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)。
文摘Plant growth and productivity are negatively affected by soil salinity.This study investigated the effects of the rhizosphere-promoting bacterium,Bacillus paramycoides JYZ-SD5,and the ectomycorrhizal fungus,Schizophyllum commune Be,on the growth of Metasequoia glyptostroboides under salt stress.Changes in biomass,root growth,root ion distribution and in vivo enzyme activities were determined under different treatments(Be,JYZ-SD5,and Be+JYZ-SD5).The results show that all inoculations increased chlorophyll content,shoot length and root diameter with or without salt stress,and the effect of Be+JYZ-SD5was the strongest.JYZ-SD5 and Be+JYZ-SD5 treatments significantly increased root length,surface area,bifurcation number,tip number,main root length and diameter under salt stress.Normal chloroplast structures developed under both single and double inoculations.Relative to the control,root activities of M.glyptostroboides in the Be,JYZSD5,and Be+JYZ-SD5 treatments increased by 31.3%,17.2%,and 33.7%.All treatments increased the activities of superoxide dismutase(SOD),peroxidase(POD),Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase.The strongest effect was by Be+JYZ-SD5.Analysis of root ion distribution showed that,under salt stress,Na^(+)and K^(+)decreased and were concentrated in the epidermis or cortex.Na/K ratios also decreased.The Be+JYZ-SD5 treatment increased betaine by 130.3%and 97.9%under 50 mM and 100 mM salt stress,respectively.Together,these changes result in the activation of physiological and biochemical processes involved in the mitigation of salinity-induced stress in M.glyptostroboides.
基金supported by the National Natural Science Foundation of China(31171823)
文摘From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune was characterized by rounded scutella, which was varied from two annules anterior to the anus and five annules posterior. The lateral field was not areolated at the level of scutella. S. magniphasma was characterized by varying scutellum, about 7.0 μm in diameter, in position from four annules posterior to four annules anterior to the anus, and areolated at the level of the scutellum.
