Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report ...Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report our experience in the treatment of a C. testosteroni-associated peritonitis in a four-year-old girl receiving chronic peritoneal dialysis(PD). The organism was shown to be highly susceptible to appropriate antibiotic therapy. Infection responded promptly and the patient was managed conservatively without withdrawal from PD.展开更多
BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MA...BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS),more cases have been identified.CASE SUMMARY This is the first report of a maternal patient with a C.kerstersii bacteremia following caesarean section.Due to the severity of the patient’s condition;high fever and rapidly progressing organ damage,the patient was transferred to the intensive care unit.C.kerstersii was detected by metagenomic next-generation sequencing testing.Based on the drug sensitivity test,appropriate antibiotic treatment was given and the patient recovered fully.CONCLUSION This case report confirms that the detection via MALDI-TOF-MS and metagenomic next-generation sequencing testing provides a reliable basis for the diagnosis of this rare bacterial infection.展开更多
The escalating crisis of polyethylene terephthalate(PET)microplastic contamination in biological wastewater treatment systems is a pressing environmental concern.These microplastics inevitably accumulate in sewage slu...The escalating crisis of polyethylene terephthalate(PET)microplastic contamination in biological wastewater treatment systems is a pressing environmental concern.These microplastics inevitably accumulate in sewage sludge due to the absence of effective removal technologies.Addressing this urgent issue,this study introduces a novel approach using DuraPETase,a potent enzyme with enhanced PET hydrolytic activity at ambient temperatures.Remarkably,this enzyme was successfully secreted from Comamonas testosteroni CNB-1,a dominant species in the active sludge.The secreted DuraPETase showed significant hydrolytic activity toward p-NPB and PET nanoplastics.Furthermore,the CNB-1 derived whole-cell biocatalyst was able to depolymerize PET microplastics under ambient temperature,achieving a degradation efficiency of 9%within 7 days.The CNB-1-based whole biocatalysts were also capable of utilizing PET degradation intermediates,such as terephthalic acid(TPA)and ethylene glycol(EG),and bis(2-hydroxyethyl)-TPA(BHET),for growth.This indicates that it can completely mineralize PET,as opposed to merely breaking it down into smaller molecules.This research highlights the potential of activated sludge as a potent source for insitu microplastic removal.展开更多
Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious inf...Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious infections like central venoug catheter associated bacleremia. corneal ulcers,otitis media exist.The microbiologists can identify this organism based on an orange indole reaction.This reaction demonstrates the organism's ability to produce anthranilic acid from tryptophan on addition of Kovac's reagent;which gives the media its characteristic "pumpkin orange" colour.Here we report the isolation of this organism from the Endotracheal tube aspirate of a 4 year old child.With the increasing use of invasive devices,it has become important to recognize these non fermentative gram negative bacilli as emerging source of infection even in immunocompetent individuals.展开更多
文摘Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report our experience in the treatment of a C. testosteroni-associated peritonitis in a four-year-old girl receiving chronic peritoneal dialysis(PD). The organism was shown to be highly susceptible to appropriate antibiotic therapy. Infection responded promptly and the patient was managed conservatively without withdrawal from PD.
文摘BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS),more cases have been identified.CASE SUMMARY This is the first report of a maternal patient with a C.kerstersii bacteremia following caesarean section.Due to the severity of the patient’s condition;high fever and rapidly progressing organ damage,the patient was transferred to the intensive care unit.C.kerstersii was detected by metagenomic next-generation sequencing testing.Based on the drug sensitivity test,appropriate antibiotic treatment was given and the patient recovered fully.CONCLUSION This case report confirms that the detection via MALDI-TOF-MS and metagenomic next-generation sequencing testing provides a reliable basis for the diagnosis of this rare bacterial infection.
基金supported by the National Key R&D Program of China(2019YFA0905500)the National Natural Science Foundation of China(21978129,31961133017)+2 种基金the European Union’s Horizon 2020 research and innovation program under grant agreement no.870294 for the project MIX-UPthe Natural Science Foundation of Jiangsu Province of China for Excellent Young Scholars(BK20211591)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTB2203).
文摘The escalating crisis of polyethylene terephthalate(PET)microplastic contamination in biological wastewater treatment systems is a pressing environmental concern.These microplastics inevitably accumulate in sewage sludge due to the absence of effective removal technologies.Addressing this urgent issue,this study introduces a novel approach using DuraPETase,a potent enzyme with enhanced PET hydrolytic activity at ambient temperatures.Remarkably,this enzyme was successfully secreted from Comamonas testosteroni CNB-1,a dominant species in the active sludge.The secreted DuraPETase showed significant hydrolytic activity toward p-NPB and PET nanoplastics.Furthermore,the CNB-1 derived whole-cell biocatalyst was able to depolymerize PET microplastics under ambient temperature,achieving a degradation efficiency of 9%within 7 days.The CNB-1-based whole biocatalysts were also capable of utilizing PET degradation intermediates,such as terephthalic acid(TPA)and ethylene glycol(EG),and bis(2-hydroxyethyl)-TPA(BHET),for growth.This indicates that it can completely mineralize PET,as opposed to merely breaking it down into smaller molecules.This research highlights the potential of activated sludge as a potent source for insitu microplastic removal.
文摘Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious infections like central venoug catheter associated bacleremia. corneal ulcers,otitis media exist.The microbiologists can identify this organism based on an orange indole reaction.This reaction demonstrates the organism's ability to produce anthranilic acid from tryptophan on addition of Kovac's reagent;which gives the media its characteristic "pumpkin orange" colour.Here we report the isolation of this organism from the Endotracheal tube aspirate of a 4 year old child.With the increasing use of invasive devices,it has become important to recognize these non fermentative gram negative bacilli as emerging source of infection even in immunocompetent individuals.
