To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectro...To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GCxGC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.展开更多
Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,co...Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,contributing to reproduction,defense against natural enemies,and adaptation to environmental changes.Thus,a deeper understanding of their leaf coloration will be essential for both practical applications and theoretical understanding.The present study comprehensively reviews the factors influencing anthocyanin metabolism,including biosynthesis,transport,degradation,transcription factors(TF_(S)),post-transcriptional regulation,post translation regulation.Next,we summarize the application of omics technologies in unveiling the mechanisms of anthocyanin synthesis in leaves.Furthermore,we review the molecular mechanisms by which environmental factors regulate leaf coloration by inducing anthocyanin biosynthesis.Lastly,the study addresses unresolved issues in the research of plant leaf coloration and proposes future research directions in this field.This study is anticipated to provide a valuable reference for the study of plant leaf coloration.展开更多
Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approa...Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.展开更多
Color filters are essential components for optical modulation.However,conventional filters are restricted to operating exclusively in either reflective or transmissive mode.Furthermore,they suffer from limited UV and ...Color filters are essential components for optical modulation.However,conventional filters are restricted to operating exclusively in either reflective or transmissive mode.Furthermore,they suffer from limited UV and thermal stability,low color purity,and exhibit identical coloration on both surfaces.Herein,we propose a novel design strategy for trans-reflective color filters by integrating the absorptive properties of dye-doped polysulfone(PSU)with the diffractive capabilities of photonic crystals.This composite filter achieved broad-spectrum transmission with deep color outputs—yellow(0.410,0.510),magenta(0.446,0.231),and cyan(0.201,0.425)—closely aligned with standard color space coordinates.By tuning the refractive index of CeO_(2)@SiO_(2)nanoparticles to match dye-based PSU matrix,the transmittance of filters exceeded 70%.Moreover,dye-mediated absorption reduces the scattering light,thereby enhancing reflection color purity(full width at half maxima(FWHM)=25 nm)and producing vibrant blue,green,and red hues.The incorporation of UV-absorbing CeO_(2)@SiO_(2)nanoparticles effectively mitigated dye photodegradation,yielding exceptional UV stability(ΔT<2%under prolonged UV exposure).The filters also exhibited outstanding thermal stability(ΔT<1%after 30 min heat treatment at 230°C).This work establishes a robust materials design framework for multifunctional optical filters,advancing the development of highfidelity dual-mode color systems for next-generation display technologies.展开更多
Fig(Ficus carica L.)with purple-red peel cultivars are popular among consumers and exhibit better storability.While DNA methylation influences fruit ripening and color development,its specific role in fig fruit remain...Fig(Ficus carica L.)with purple-red peel cultivars are popular among consumers and exhibit better storability.While DNA methylation influences fruit ripening and color development,its specific role in fig fruit remains unclear.This study explores the impact of DNA methylation on the fig peel coloration.Enzymatic colorimetric detection revealed that the level of‘Purple Peel’fig DNA methylation decreases with fig fruit ripening and coloring.Treatment of young fruit with the DNA-methylation inhibitor azacytidine induced peel coloration,suggesting that a decrease in DNA-methylation level promotes fig peel coloration.Seven members of DNA methyltransferases and three members of DNA demethylases were identified from a high-level fig genome,highlighting FcMET1 and FcDRM2 as stable proteins,ensuring functional expression.Reference to the Arabidopsis protein interaction network map predicted that FcMET1 is in a central position,suggesting a crucial regulatory role in multiple biological processes.Correlation analysis revealed a positive correlation between FcMET1 expression during peel development and the level of total DNA methylation.Weighted gene co-expression network analysis identified co-expression of FcMET1 with the color-related transcription factors MYB,bHLH and WD40,as well as with eight structural genes in the flavonoid-biosynthesis pathway.The expression of FcUFGT3 was negatively correlated with that of FcMET1.McrBC-PCR and Bisulfite Sequencing detection showed that a low methylation level of the FcUFGT3 promoter corresponds with its high expression in colored fig.This investigation of the mechanism of DNA methylation provides a theoretical basis for understanding the role of DNA-methylation modifications in fig ripening and coloring.展开更多
Flexible and wearable sensors offer immense potential for rehabilitation medicine,but most rely solely on electrical signals,lacking real-time visual feedback and limiting trainee's interactivity.Inspired by the s...Flexible and wearable sensors offer immense potential for rehabilitation medicine,but most rely solely on electrical signals,lacking real-time visual feedback and limiting trainee's interactivity.Inspired by the structural coloration of Cyanocitta stelleri feathers,we developed a dual-mode sensor by utilizing black conductive polymer hydrogel(CPH)-enhanced structural color strategy.This sensor integrates a hydroxypropyl cellulose(HPC)-based structural color interface with a designed CPH sensing component.Highly visible light-absorbing CPH(absorption rate>88%)serves as the critical substrate for enhancing structural color performance.By absorbing incoherent scattered light and suppressing background interference,it significantly enhances the saturation of structural color,thereby achieving a high contrast index of 4.92.Unlike the faint and hardly visible structural colors on non-black substrates,the HPC on CPH displays vivid,highly perceptible colors and desirable mechanochromic behavior.Moreover,the CPH acts as a flexible sensing element,fortified by hydrogen and coordination bond networks,and exhibits exceptional electromechanical properties,including 867.1 kPa tensile strength,strain sensitivity(gauge factor of 4.24),and outstanding durability(over 4400 cycles).Compared to traditional single-mode sensors,the integrated sensor provides real-time visual and digital dual feedback,enhancing the accuracy and interactivity of rehabilitation assessments.This technology holds promise for advancing next-generation rehabilitation medicine.展开更多
Frontiers of Nursing is a nursing academic journal,CN 14-1395/R,ISSN 2097-5368.Size A4,quarterly publication,color printing,public offerings at home and abroad which is managed by the First Hospital of Shanxi Medical ...Frontiers of Nursing is a nursing academic journal,CN 14-1395/R,ISSN 2097-5368.Size A4,quarterly publication,color printing,public offerings at home and abroad which is managed by the First Hospital of Shanxi Medical University,sponsored by Shanxi Nursing Association and Shanxi Medical Periodical Press Co.,Ltd.Frontiers of Nursing has been indexed by databases including Scopus,Google Scholar,DOAJ,Baidu Scholar,CNKI,VIP,Wanfang,Chaoxing,and so on.展开更多
Carotenoids are natural pigments that are widely distributed in the flowers,fruits,and seeds of many plant species.These compounds not only endow diverse colors but also exhibit antioxidant,immune-modulatory,anti-agin...Carotenoids are natural pigments that are widely distributed in the flowers,fruits,and seeds of many plant species.These compounds not only endow diverse colors but also exhibit antioxidant,immune-modulatory,anti-aging,and photoprotective properties.Although carotenoid metabolism has been studied extensively in microbial and plant science,the genetic mechanisms underlying carotenoid metabolism in cotton remain underexplored.Here,we isolated gene GbDYA that regulates a dark-yellow anther color by map-based cloning using a BC1F1 population derived from a cross of Gossypium barbadense acc.Hai7124 with dark-yellow anthers and G.hirsutum acc.TM-1 with light-yellow anthers backcrossed with TM-1.GbDYA encodes phytoene synthase,a key rate-limiting enzyme in the carotenoid biosynthesis pathway.A long terminal repeat retrotransposon in the first exon of GhDYA(an ortholog of GbDYA in G.hirsutum acc.TM-1)caused loss of function and led to the light-yellow anther color.GbDYA is predominantly expressed in the early stages of anther development.Transcriptome,RT-qPCR and KEGG enrichment analyses revealed that GbDYA influences the synthesis and accumulation of carotenoids in anthers by modulating expression of key genes in the carotenoid biosynthesis pathway.Integrated transcriptomic and metabolomic analyses indicated that the accumulation of lutein,violaxanthin,antherxanthin,cryptoxanthin,zeaxanthin,andβ-carotene contributed to yellow coloration of anthers.Dual-luciferase and yeast one-hybrid assays confirmed that transcription factor GbMYB105(GB_A11G3511)binds to the promoter of GbDYA and activates its expression.High-temperature stress treatment indicated that carotenoids accumulation in anthers enhances pollen antioxidant activity.This study unravels the role of GbDYA in conferring the anther coloration,and provides the potential utilization by modulating accumulation of carotenoids in anthers to enhance pollen viability in high-temperature tolerance breeding in cotton.展开更多
Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spec...Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spectrum.In this study,we successfully engineered the carotenoid biosynthesis pathway specifically in cotton fibers by utilizing a fiber-specific GbEXPA2 promoter and a CaMV 35S promoter to drive the expression of two key carotenoid biosynthesis genes,CrtB and CrtI,respectively.This approach resulted in the development of a golden fiber cotton germplasm enriched withβ-carotene.Notably,the pigmentation was predominantly observed during the early developmental stages of the fiber(5–20 d post-anthesis).While the presence of carotenoids had no significant effect on plant architecture and growth,it positively influenced the fiber elongation rate,albeit with a slight reduction in fiber length and strength.This study represents a pioneering strategy for the future development of NCCs through carotenoid biofortification.展开更多
Flower color is an essential trait in ornamental plant breeding. Lycoris longituba is a popular ornamental plant native to central eastern China. The decrease in anthocyanin accumulation causes L. longituba petal colo...Flower color is an essential trait in ornamental plant breeding. Lycoris longituba is a popular ornamental plant native to central eastern China. The decrease in anthocyanin accumulation causes L. longituba petal color fading during flower development, which considerably affects the ornamental value of L. longituba. However, mechanisms underlying anthocyanin biosynthesis inhibition during L. longituba petal development remain unclear. In this study, three LlDFR genes were confirmed to be involved in anthocyanin biosynthesis and LlDFRc exerted the strongest promoting effect on anthocyanin accumulation. According to the correlation analysis results, LlbHLH12 exhibited the strongest negative correlation with LlDFRc. Quantitative real-time PCR analysis showed that LlbHLH12 was highly expressed during the medium bud and full bloom stages of flower development. LlbHLH12 was identified as a member of subgroup XII of bHLH transcription factor family. Subcellular localization and transcriptional activation ability assay revealed that LlbHLH12 was located in the nucleus without transcriptional activation activity. Overexpression of LlbHLH12 in Nicotiana tabacum and L. longituba inhibited anthocyanin accumulation by suppressing the expression of anthocyanin biosynthetic pathway genes. Furthermore, yeast one-hybrid, dual-luciferase, and β-glucuronidase activity assays showed that LlbHLH12 directly bound to the promoters of LlPAL and LlDFRc and suppressed their expression to inhibit anthocyanin biosynthesis. Overall, our study identified a novel bHLH repressor negatively regulating anthocyanin biosynthesis and provided new insights into the molecular mechanisms underlying color fading in L. longituba petals.展开更多
A t-tone coloring of a graph assigns t distinct colors to each vertex with vertices at distance d having fewer than d colors in common.The t-tone chromatic number of a graph is the smallest number of colors used in al...A t-tone coloring of a graph assigns t distinct colors to each vertex with vertices at distance d having fewer than d colors in common.The t-tone chromatic number of a graph is the smallest number of colors used in all t-tone colorings of that graph.In this article,we study t-tone coloring of some finite planar lattices and obtain exact formulas for their t-tone chromatic number.展开更多
By leveraging the unique qualities of microorganisms,engineered living materials(ELMs)offer functional and economic advantages in everyday applications along with notable ecological benefits.This study contributes to ...By leveraging the unique qualities of microorganisms,engineered living materials(ELMs)offer functional and economic advantages in everyday applications along with notable ecological benefits.This study contributes to the growing field of biodesign by examining the potential of Flavobacteria for thermochromic ELMs.Many Flavobacteria,commonly found in marine environments,produce iridescent structural colorations as their colonies expand on semi-solid surfaces through gliding motility.In this study,we analyzed the effects of temperature variations on flavobacterium Cellulophaga lytica PLY A 2,characterizing distinct changes in colony growth and iridescent colorations at a macroscopic and microscopic scale.Using scanning electron microscopy,we investigated the relationship between iridescent color and the underlying cell-based optical structures.By providing insights into the temperature-responsive behavior of Flavobacteria,our findings highlight their potential for future thermochromic ELMs-with applications ranging from sustainable food packaging to smart textiles-while encouraging further characterization studies within biodesign research.展开更多
Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challengin...Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challenging due to its large genome size and high proportion of repetitive sequences.Allele-specific expression(ASE)plays a key role in regulating plant development and evolution,yet research on ASE in coconut is limited(Shao et al.,2019;Li et al.,2021;Zhang et al.,2021;Hu et al.,2022).Among phenotypic traits,fruit color is especially important as an indicator of maturity,guiding harvest timing and post-harvest processes(Kapoor et al.,2022).While prior studies have explored various coconut traits such as salt tolerance,fiber content,and plant height(Wang et al.,2021;Yang et al.,2021),investigations into ASE and fruit color remain scarce.展开更多
Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Ter...Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Terpenoids are important secondary metabolites in plants,with β-carotene(C40)being the major pigment substance and linalool(C10)being the key aromatic component in O.fragrans.The geranylgeranyl pyrophosphate synthase genes(GGPPSs)play important roles in secondary metabolism in plants.However,the functions of the GGPPS family in floral color and fragrance formation has rarely been reported in O.fragrans.In this study,24 Of GGPPS genes were identified and classified into two subfamilies.The Of GGPPSs showed tissue-specific expression and Of GGPPS13 had highest expression in flowers.The Of GGPPS13 protein was localized to chloroplasts.The transcriptome data of Of GGPPS13 was verified by q RT-PCR and the expression level in‘Wanyingui'with strong aroma was higher than that in‘Zhuangyuanhong'with deep color at different flower development stages.Transient overexpression of Of GGPPS13 in O.fragrans petals showed that Of GGPPS13 increased the β-carotene content,the main color substance of O.fragrans,but decreased the linalool content,the main volatile organic compound(VOC)in the floral aroma of O.fragrans.Of GGPPS13 was indicated as the critical gene related to terpenoid synthesis in the floral aroma and color formation in O.fragrans.Our findings provide gene resources on the GGPPS gene family for further revealing the molecular regulation mechanism of the floral color and aroma formation in O.fragrans.展开更多
Advances in software and hardware technologies have facilitated the production of quadrotor unmanned aerial vehicles(UAVs).Nowadays,people actively use quadrotor UAVs in essential missions such as search and rescue,co...Advances in software and hardware technologies have facilitated the production of quadrotor unmanned aerial vehicles(UAVs).Nowadays,people actively use quadrotor UAVs in essential missions such as search and rescue,counter-terrorism,firefighting,surveillance,and cargo transportation.While performing these tasks,quadrotors must operate in noisy environments.Therefore,a robust controller design that can control the altitude and attitude of the quadrotor in noisy environments is of great importance.Many researchers have focused only on white Gaussian noise in their studies,whereas researchers need to consider the effects of all colored noises during the operation of the quadrotor.This study aims to design a robust controller that is resistant to all colored noises.Firstly,a nonlinear quadrotormodel was created with MATLAB.Then,a backstepping controller resistant to colored noises was designed.Thedesigned backstepping controller was tested under Gaussian white,pink,brown,blue,and purple noises.PID and Lyapunov-based controller designswere also carried out,and their time responses(rise time,overshoot,settling time)were compared with those of the backstepping controller.In the simulations,time was in seconds,altitude was in meters,and roll,pitch,and yaw references were in radians.Rise and settling time values were in seconds,and overshoot value was in percent.When the obtained values are examined,simulations prove that the proposed backstepping controller has the least overshoot and the shortest settling time under all noise types.展开更多
It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be...It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.展开更多
Pepper(Capsicum annuum L.)is a typical self-pollinating crop with obvious heterosis in hybrids.Consequently,the use of morphological markers during the pepper seedling stage is crucial for pepper breeding.The color of...Pepper(Capsicum annuum L.)is a typical self-pollinating crop with obvious heterosis in hybrids.Consequently,the use of morphological markers during the pepper seedling stage is crucial for pepper breeding.The color of hypocotyl is widely used as a phenotypic marker in crossing studies of pepper.Pepper accessions generally have purple hypocotyls,which are mainly due to the anthocyanin accumulation in seedlings,and green hypocotyls are rarely observed in pepper.Here we reported the characterization of a green hypocotyl mutant of pepper,Cha1,which was identified from a pepper ethyl methanesulfonate(EMS)mutant library.Fine mapping revealed that the causal gene,CaTTG1,belonging to the WD40 repeat family,controlled the green hypocotyl phenotype of the mutant.Virus-induced gene silencing(VIGS)confirmed that CaTTG1 regulated anthocyanin accumulation.RNA-seq data showed that expression of structural genes CaDFR,CaANS,and CaUF3GT in the anthocyanin biosynthetic pathway was significantly decreased in Cha1 compared to the wild type.Yeast two-hybrid(Y2H)experiments also confirmed that CaTTG1 activated the synthesis of anthocyanin structural genes by forming a MBW complex with CaAN1 and CaGL3.In summary,this study provided a green hypocotyl mutant of pepper,and the Kompetitive Allele Specific PCR(KASP)marker developed based on the mutation site of the underlying gene would be helpful for pepper breeding.展开更多
Super-fine electrohydrodynamic inkjet(SIJ)printing of perovskite nanocrystal(PNC)colloid ink exhibits significant potential in the fabrication of high-resolution color conversion microstructures arrays for fullcolor m...Super-fine electrohydrodynamic inkjet(SIJ)printing of perovskite nanocrystal(PNC)colloid ink exhibits significant potential in the fabrication of high-resolution color conversion microstructures arrays for fullcolor micro-LED displays.However,the impact of solvent on both the printing process and the morphology of SIJ-printed PNC color conversion microstructures remains underexplored.In this study,we prepared samples of CsPbBr3PNC colloid inks in various solvents and investigated the solvent's impact on SIJ printed PNC microstructures.Our findings reveal that the boiling point of the solvent is crucial to the SIJ printing process of PNC colloid inks.Only does the boiling point of the solvent fall in the optimal range,the regular positioned,micron-scaled,conical PNC microstructures can be successfully printed.Below this optimal range,the ink is unable to be ejected from the nozzle;while above this range,irregular positioned microstructures with nanoscale height and coffee-ring-like morphology are produced.Based on these observations,high-resolution color conversion PNC microstructures were effectively prepared using SIJ printing of PNC colloid ink dispersed in dimethylbenzene solvent.展开更多
Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution fo...Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution for high-throughput digital pathology,combining high resolution,large field of view,and extended depth of field(DOF).However,the full-color capabilities of FPM are hindered by coherent color artifacts and reduced computational efficiency,which significantly limits its practical applications.Color-transferbased FPM(CFPM)has emerged as a potential solution,theoretically reducing both acquisition and reconstruction threefold time.Yet,existing methods fall short of achieving the desired reconstruction speed and colorization quality.In this study,we report a generalized dual-color-space constrained model for FPM colorization.This model provides a mathematical framework for model-based FPM colorization,enabling a closed-form solution without the need for redundant iterative calculations.Our approach,termed generalized CFPM(gCFPM),achieves colorization within seconds for megapixel-scale images,delivering superior colorization quality in terms of both colorfulness and sharpness,along with an extended DOF.Both simulations and experiments demonstrate that gCFPM surpasses state-of-the-art methods across all evaluated criteria.Our work offers a robust and comprehensive workflow for high-throughput full-color pathological imaging using FPM platforms,laying a solid foundation for future advancements in methodology and engineering.展开更多
基金Supported by the National Natural Science Foundation of China(No.81173546 and No.30940093)the Natural Science Foundation of Jiangsu Province,China(No.BK2009495)+4 种基金the International Science and Technology Cooperation Project of Jiangsu Province,China(No.BZ2011053)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,No.2011ZYX2-001)the Postgraduate Research Innovation Project of Jiangsu Higher Education Institutions(No.KYLX_0975)the Chinese Medicine Research Program of Zhejiang Province,China(No.2014ZQ008)the Science Foundation of Zhejiang Chinese Medical University(No.2013ZZ12)
文摘To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. A comprehensive two-dimensional gas chromatography (GCxGC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GCxGC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.
基金supported by Central Finance for the Forestry Science and Technology Promotion Demonstration Project([2024]TG13)the National Science Foundation of China(Grant No.32201643)the Key research projects of Yibin,Research and Integrated Demonstration and Key Technologies for Smart Bamboo Industry(Grant No.YBZD2024-1).
文摘Anthocyanin-rich foliage plants hold important applications in the pharmaceutical industry and the tea sector,beyond their significant ornamental value.These plants also possess biological and ecological importance,contributing to reproduction,defense against natural enemies,and adaptation to environmental changes.Thus,a deeper understanding of their leaf coloration will be essential for both practical applications and theoretical understanding.The present study comprehensively reviews the factors influencing anthocyanin metabolism,including biosynthesis,transport,degradation,transcription factors(TF_(S)),post-transcriptional regulation,post translation regulation.Next,we summarize the application of omics technologies in unveiling the mechanisms of anthocyanin synthesis in leaves.Furthermore,we review the molecular mechanisms by which environmental factors regulate leaf coloration by inducing anthocyanin biosynthesis.Lastly,the study addresses unresolved issues in the research of plant leaf coloration and proposes future research directions in this field.This study is anticipated to provide a valuable reference for the study of plant leaf coloration.
基金funding from the Natural Science Foundation of Sichuan Province,China(Grant Nos.2025ZNSFSC1100,2024NSFSC0324)the National Key Research and Development Project(Grant No.2017YFC0505104)+2 种基金the Sichuan Agricultural University Dual Support Plan Special Project(Grant No.2024ZYTS020)the Postdoctoral Fellowship Program(Grade B)of China Postdoctoral Science Foundation(Grant No.GZB20250863)the China Postdoctoral Science Foundation(Grant No.2025M773740).
文摘Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.
基金supported by the Program of the National Natural Science Foundation of China(22238002)the Fundamental Research Funds for the Central Universities(DUT22-LAB610)Research and Innovation Team Project of Dalian University of Technology(DUT2022TB10).
文摘Color filters are essential components for optical modulation.However,conventional filters are restricted to operating exclusively in either reflective or transmissive mode.Furthermore,they suffer from limited UV and thermal stability,low color purity,and exhibit identical coloration on both surfaces.Herein,we propose a novel design strategy for trans-reflective color filters by integrating the absorptive properties of dye-doped polysulfone(PSU)with the diffractive capabilities of photonic crystals.This composite filter achieved broad-spectrum transmission with deep color outputs—yellow(0.410,0.510),magenta(0.446,0.231),and cyan(0.201,0.425)—closely aligned with standard color space coordinates.By tuning the refractive index of CeO_(2)@SiO_(2)nanoparticles to match dye-based PSU matrix,the transmittance of filters exceeded 70%.Moreover,dye-mediated absorption reduces the scattering light,thereby enhancing reflection color purity(full width at half maxima(FWHM)=25 nm)and producing vibrant blue,green,and red hues.The incorporation of UV-absorbing CeO_(2)@SiO_(2)nanoparticles effectively mitigated dye photodegradation,yielding exceptional UV stability(ΔT<2%under prolonged UV exposure).The filters also exhibited outstanding thermal stability(ΔT<1%after 30 min heat treatment at 230°C).This work establishes a robust materials design framework for multifunctional optical filters,advancing the development of highfidelity dual-mode color systems for next-generation display technologies.
基金supported by 111 Project(Grant No.B17043)China Postdoctoral Science Foundation(Grant No.2022M723425).
文摘Fig(Ficus carica L.)with purple-red peel cultivars are popular among consumers and exhibit better storability.While DNA methylation influences fruit ripening and color development,its specific role in fig fruit remains unclear.This study explores the impact of DNA methylation on the fig peel coloration.Enzymatic colorimetric detection revealed that the level of‘Purple Peel’fig DNA methylation decreases with fig fruit ripening and coloring.Treatment of young fruit with the DNA-methylation inhibitor azacytidine induced peel coloration,suggesting that a decrease in DNA-methylation level promotes fig peel coloration.Seven members of DNA methyltransferases and three members of DNA demethylases were identified from a high-level fig genome,highlighting FcMET1 and FcDRM2 as stable proteins,ensuring functional expression.Reference to the Arabidopsis protein interaction network map predicted that FcMET1 is in a central position,suggesting a crucial regulatory role in multiple biological processes.Correlation analysis revealed a positive correlation between FcMET1 expression during peel development and the level of total DNA methylation.Weighted gene co-expression network analysis identified co-expression of FcMET1 with the color-related transcription factors MYB,bHLH and WD40,as well as with eight structural genes in the flavonoid-biosynthesis pathway.The expression of FcUFGT3 was negatively correlated with that of FcMET1.McrBC-PCR and Bisulfite Sequencing detection showed that a low methylation level of the FcUFGT3 promoter corresponds with its high expression in colored fig.This investigation of the mechanism of DNA methylation provides a theoretical basis for understanding the role of DNA-methylation modifications in fig ripening and coloring.
基金supported by the Science and Technology Development Fund,Macao SAR(0065/2023/AFJ,0116/2022/A3)the National Natural Science Foundation of China(52402166)+4 种基金the Natural Science Foundation of Guangdong Province(2025A1515011120)the Australian Research Council(DE220100154)the financial support from the Science and Technology Development Fund(FDCT),Macao SAR(No.0149/2022/A),and(No.0046/2024/AFJ)Guangdong Science and Technology Department(2023QN10C305)for this workthe financial support from the National Natural Science Foundation of China(Grant No.22305185)。
文摘Flexible and wearable sensors offer immense potential for rehabilitation medicine,but most rely solely on electrical signals,lacking real-time visual feedback and limiting trainee's interactivity.Inspired by the structural coloration of Cyanocitta stelleri feathers,we developed a dual-mode sensor by utilizing black conductive polymer hydrogel(CPH)-enhanced structural color strategy.This sensor integrates a hydroxypropyl cellulose(HPC)-based structural color interface with a designed CPH sensing component.Highly visible light-absorbing CPH(absorption rate>88%)serves as the critical substrate for enhancing structural color performance.By absorbing incoherent scattered light and suppressing background interference,it significantly enhances the saturation of structural color,thereby achieving a high contrast index of 4.92.Unlike the faint and hardly visible structural colors on non-black substrates,the HPC on CPH displays vivid,highly perceptible colors and desirable mechanochromic behavior.Moreover,the CPH acts as a flexible sensing element,fortified by hydrogen and coordination bond networks,and exhibits exceptional electromechanical properties,including 867.1 kPa tensile strength,strain sensitivity(gauge factor of 4.24),and outstanding durability(over 4400 cycles).Compared to traditional single-mode sensors,the integrated sensor provides real-time visual and digital dual feedback,enhancing the accuracy and interactivity of rehabilitation assessments.This technology holds promise for advancing next-generation rehabilitation medicine.
文摘Frontiers of Nursing is a nursing academic journal,CN 14-1395/R,ISSN 2097-5368.Size A4,quarterly publication,color printing,public offerings at home and abroad which is managed by the First Hospital of Shanxi Medical University,sponsored by Shanxi Nursing Association and Shanxi Medical Periodical Press Co.,Ltd.Frontiers of Nursing has been indexed by databases including Scopus,Google Scholar,DOAJ,Baidu Scholar,CNKI,VIP,Wanfang,Chaoxing,and so on.
基金financially supported in part by grants from Biological Breeding-National Science and Technology Major Project (2023ZD04040)Jiangsu Key Research and Development Program (BE2022384)Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry (CICMCP) (No. 10)
文摘Carotenoids are natural pigments that are widely distributed in the flowers,fruits,and seeds of many plant species.These compounds not only endow diverse colors but also exhibit antioxidant,immune-modulatory,anti-aging,and photoprotective properties.Although carotenoid metabolism has been studied extensively in microbial and plant science,the genetic mechanisms underlying carotenoid metabolism in cotton remain underexplored.Here,we isolated gene GbDYA that regulates a dark-yellow anther color by map-based cloning using a BC1F1 population derived from a cross of Gossypium barbadense acc.Hai7124 with dark-yellow anthers and G.hirsutum acc.TM-1 with light-yellow anthers backcrossed with TM-1.GbDYA encodes phytoene synthase,a key rate-limiting enzyme in the carotenoid biosynthesis pathway.A long terminal repeat retrotransposon in the first exon of GhDYA(an ortholog of GbDYA in G.hirsutum acc.TM-1)caused loss of function and led to the light-yellow anther color.GbDYA is predominantly expressed in the early stages of anther development.Transcriptome,RT-qPCR and KEGG enrichment analyses revealed that GbDYA influences the synthesis and accumulation of carotenoids in anthers by modulating expression of key genes in the carotenoid biosynthesis pathway.Integrated transcriptomic and metabolomic analyses indicated that the accumulation of lutein,violaxanthin,antherxanthin,cryptoxanthin,zeaxanthin,andβ-carotene contributed to yellow coloration of anthers.Dual-luciferase and yeast one-hybrid assays confirmed that transcription factor GbMYB105(GB_A11G3511)binds to the promoter of GbDYA and activates its expression.High-temperature stress treatment indicated that carotenoids accumulation in anthers enhances pollen antioxidant activity.This study unravels the role of GbDYA in conferring the anther coloration,and provides the potential utilization by modulating accumulation of carotenoids in anthers to enhance pollen viability in high-temperature tolerance breeding in cotton.
基金supported by grants from the National Natural Science Foundation of China(32170271,32470277)the Natural Science Foundation of Henan Province(222300420024).
文摘Natural colored cotton(NCC)offers a sustainable,dye-free,and eco-friendly alternative for producing colored textiles.Carotenoids,a group of important natural liposoluble pigments,are known for their diverse color spectrum.In this study,we successfully engineered the carotenoid biosynthesis pathway specifically in cotton fibers by utilizing a fiber-specific GbEXPA2 promoter and a CaMV 35S promoter to drive the expression of two key carotenoid biosynthesis genes,CrtB and CrtI,respectively.This approach resulted in the development of a golden fiber cotton germplasm enriched withβ-carotene.Notably,the pigmentation was predominantly observed during the early developmental stages of the fiber(5–20 d post-anthesis).While the presence of carotenoids had no significant effect on plant architecture and growth,it positively influenced the fiber elongation rate,albeit with a slight reduction in fiber length and strength.This study represents a pioneering strategy for the future development of NCCs through carotenoid biofortification.
基金supported by the National Natural Science Foundation of China(Grant Nos.31870695,32071828)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Flower color is an essential trait in ornamental plant breeding. Lycoris longituba is a popular ornamental plant native to central eastern China. The decrease in anthocyanin accumulation causes L. longituba petal color fading during flower development, which considerably affects the ornamental value of L. longituba. However, mechanisms underlying anthocyanin biosynthesis inhibition during L. longituba petal development remain unclear. In this study, three LlDFR genes were confirmed to be involved in anthocyanin biosynthesis and LlDFRc exerted the strongest promoting effect on anthocyanin accumulation. According to the correlation analysis results, LlbHLH12 exhibited the strongest negative correlation with LlDFRc. Quantitative real-time PCR analysis showed that LlbHLH12 was highly expressed during the medium bud and full bloom stages of flower development. LlbHLH12 was identified as a member of subgroup XII of bHLH transcription factor family. Subcellular localization and transcriptional activation ability assay revealed that LlbHLH12 was located in the nucleus without transcriptional activation activity. Overexpression of LlbHLH12 in Nicotiana tabacum and L. longituba inhibited anthocyanin accumulation by suppressing the expression of anthocyanin biosynthetic pathway genes. Furthermore, yeast one-hybrid, dual-luciferase, and β-glucuronidase activity assays showed that LlbHLH12 directly bound to the promoters of LlPAL and LlDFRc and suppressed their expression to inhibit anthocyanin biosynthesis. Overall, our study identified a novel bHLH repressor negatively regulating anthocyanin biosynthesis and provided new insights into the molecular mechanisms underlying color fading in L. longituba petals.
基金Supported by the National Natural Science Foundation of China(Grant No.12271210)the Scientific Research Foundation of Jimei University(Grant No.Q202201).
文摘A t-tone coloring of a graph assigns t distinct colors to each vertex with vertices at distance d having fewer than d colors in common.The t-tone chromatic number of a graph is the smallest number of colors used in all t-tone colorings of that graph.In this article,we study t-tone coloring of some finite planar lattices and obtain exact formulas for their t-tone chromatic number.
基金partial support from the Living Circular Labels project,funded by the Taskforce for Applied Research SIA’s KIEM programme(No.CIE.06.007)in the Netherlands。
文摘By leveraging the unique qualities of microorganisms,engineered living materials(ELMs)offer functional and economic advantages in everyday applications along with notable ecological benefits.This study contributes to the growing field of biodesign by examining the potential of Flavobacteria for thermochromic ELMs.Many Flavobacteria,commonly found in marine environments,produce iridescent structural colorations as their colonies expand on semi-solid surfaces through gliding motility.In this study,we analyzed the effects of temperature variations on flavobacterium Cellulophaga lytica PLY A 2,characterizing distinct changes in colony growth and iridescent colorations at a macroscopic and microscopic scale.Using scanning electron microscopy,we investigated the relationship between iridescent color and the underlying cell-based optical structures.By providing insights into the temperature-responsive behavior of Flavobacteria,our findings highlight their potential for future thermochromic ELMs-with applications ranging from sustainable food packaging to smart textiles-while encouraging further characterization studies within biodesign research.
基金supported by Central Public-interest Scientific Institution Basal Research Fund(CATAS-Nos.1630152023007,1630152023011,1630152023012,1630152023013)the National Natural Science Foundation of China(Grant No.32071805).
文摘Coconut(Cocos nucifera L.),a major oil and fruit crop of the Arecaceae family,is extensively cultivated across the Asia—Pacific region.Despite its agricultural importance,genome assembly in coconut remains challenging due to its large genome size and high proportion of repetitive sequences.Allele-specific expression(ASE)plays a key role in regulating plant development and evolution,yet research on ASE in coconut is limited(Shao et al.,2019;Li et al.,2021;Zhang et al.,2021;Hu et al.,2022).Among phenotypic traits,fruit color is especially important as an indicator of maturity,guiding harvest timing and post-harvest processes(Kapoor et al.,2022).While prior studies have explored various coconut traits such as salt tolerance,fiber content,and plant height(Wang et al.,2021;Yang et al.,2021),investigations into ASE and fruit color remain scarce.
基金funded by the National Natural Science Foundation of China(32071828 and 32471943)the Central Finance Forestry Science and Technology Promotion Demonstration Project,China(Su2024TG04)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Chinapartly supported by the open funds of the National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,China。
文摘Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Terpenoids are important secondary metabolites in plants,with β-carotene(C40)being the major pigment substance and linalool(C10)being the key aromatic component in O.fragrans.The geranylgeranyl pyrophosphate synthase genes(GGPPSs)play important roles in secondary metabolism in plants.However,the functions of the GGPPS family in floral color and fragrance formation has rarely been reported in O.fragrans.In this study,24 Of GGPPS genes were identified and classified into two subfamilies.The Of GGPPSs showed tissue-specific expression and Of GGPPS13 had highest expression in flowers.The Of GGPPS13 protein was localized to chloroplasts.The transcriptome data of Of GGPPS13 was verified by q RT-PCR and the expression level in‘Wanyingui'with strong aroma was higher than that in‘Zhuangyuanhong'with deep color at different flower development stages.Transient overexpression of Of GGPPS13 in O.fragrans petals showed that Of GGPPS13 increased the β-carotene content,the main color substance of O.fragrans,but decreased the linalool content,the main volatile organic compound(VOC)in the floral aroma of O.fragrans.Of GGPPS13 was indicated as the critical gene related to terpenoid synthesis in the floral aroma and color formation in O.fragrans.Our findings provide gene resources on the GGPPS gene family for further revealing the molecular regulation mechanism of the floral color and aroma formation in O.fragrans.
文摘Advances in software and hardware technologies have facilitated the production of quadrotor unmanned aerial vehicles(UAVs).Nowadays,people actively use quadrotor UAVs in essential missions such as search and rescue,counter-terrorism,firefighting,surveillance,and cargo transportation.While performing these tasks,quadrotors must operate in noisy environments.Therefore,a robust controller design that can control the altitude and attitude of the quadrotor in noisy environments is of great importance.Many researchers have focused only on white Gaussian noise in their studies,whereas researchers need to consider the effects of all colored noises during the operation of the quadrotor.This study aims to design a robust controller that is resistant to all colored noises.Firstly,a nonlinear quadrotormodel was created with MATLAB.Then,a backstepping controller resistant to colored noises was designed.Thedesigned backstepping controller was tested under Gaussian white,pink,brown,blue,and purple noises.PID and Lyapunov-based controller designswere also carried out,and their time responses(rise time,overshoot,settling time)were compared with those of the backstepping controller.In the simulations,time was in seconds,altitude was in meters,and roll,pitch,and yaw references were in radians.Rise and settling time values were in seconds,and overshoot value was in percent.When the obtained values are examined,simulations prove that the proposed backstepping controller has the least overshoot and the shortest settling time under all noise types.
文摘It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display.
基金supported by grants from the Special Funds for Construction of Innovative Provinces in Hunan Province(Grant No.2021NK1006)the Science and Technology Innovation Program of Hunan Province(Grant No.2021JC0007)+2 种基金China Agriculture Research System of MOF and MARA(Grant No.CARS-24-A-15)National Natural Science Foundation of China(Grant No.32130097)National Natural Science Foundation of China(Grant No.U19A2028)。
文摘Pepper(Capsicum annuum L.)is a typical self-pollinating crop with obvious heterosis in hybrids.Consequently,the use of morphological markers during the pepper seedling stage is crucial for pepper breeding.The color of hypocotyl is widely used as a phenotypic marker in crossing studies of pepper.Pepper accessions generally have purple hypocotyls,which are mainly due to the anthocyanin accumulation in seedlings,and green hypocotyls are rarely observed in pepper.Here we reported the characterization of a green hypocotyl mutant of pepper,Cha1,which was identified from a pepper ethyl methanesulfonate(EMS)mutant library.Fine mapping revealed that the causal gene,CaTTG1,belonging to the WD40 repeat family,controlled the green hypocotyl phenotype of the mutant.Virus-induced gene silencing(VIGS)confirmed that CaTTG1 regulated anthocyanin accumulation.RNA-seq data showed that expression of structural genes CaDFR,CaANS,and CaUF3GT in the anthocyanin biosynthetic pathway was significantly decreased in Cha1 compared to the wild type.Yeast two-hybrid(Y2H)experiments also confirmed that CaTTG1 activated the synthesis of anthocyanin structural genes by forming a MBW complex with CaAN1 and CaGL3.In summary,this study provided a green hypocotyl mutant of pepper,and the Kompetitive Allele Specific PCR(KASP)marker developed based on the mutation site of the underlying gene would be helpful for pepper breeding.
基金supported by the National Natural Science Foundation of China(No.62374142)Fundamental Research Funds for the Central Universities(Nos.20720220085 and 20720240064)+2 种基金External Cooperation Program of Fujian(No.2022I0004)Major Science and Technology Project of Xiamen in China(No.3502Z20191015)Xiamen Natural Science Foundation Youth Project(No.3502Z202471002)。
文摘Super-fine electrohydrodynamic inkjet(SIJ)printing of perovskite nanocrystal(PNC)colloid ink exhibits significant potential in the fabrication of high-resolution color conversion microstructures arrays for fullcolor micro-LED displays.However,the impact of solvent on both the printing process and the morphology of SIJ-printed PNC color conversion microstructures remains underexplored.In this study,we prepared samples of CsPbBr3PNC colloid inks in various solvents and investigated the solvent's impact on SIJ printed PNC microstructures.Our findings reveal that the boiling point of the solvent is crucial to the SIJ printing process of PNC colloid inks.Only does the boiling point of the solvent fall in the optimal range,the regular positioned,micron-scaled,conical PNC microstructures can be successfully printed.Below this optimal range,the ink is unable to be ejected from the nozzle;while above this range,irregular positioned microstructures with nanoscale height and coffee-ring-like morphology are produced.Based on these observations,high-resolution color conversion PNC microstructures were effectively prepared using SIJ printing of PNC colloid ink dispersed in dimethylbenzene solvent.
基金supported by the National Natural Science Foundation of China(Grant Nos.12104500 and 82430062)the Key Research and Development Projects of Shaanxi Province(Grant No.2023-YBSF-263),the Shenzhen Engineering Research Centre(Grant No.XMHT20230115004)the Shenzhen Science and Technology Innovation Commission(Grant No.KCXFZ20201221173207022).
文摘Full-color imaging is essential in digital pathology for accurate tissue analysis.Utilizing advanced optical modulation and phase retrieval algorithms,Fourier ptychographic microscopy(FPM)offers a powerful solution for high-throughput digital pathology,combining high resolution,large field of view,and extended depth of field(DOF).However,the full-color capabilities of FPM are hindered by coherent color artifacts and reduced computational efficiency,which significantly limits its practical applications.Color-transferbased FPM(CFPM)has emerged as a potential solution,theoretically reducing both acquisition and reconstruction threefold time.Yet,existing methods fall short of achieving the desired reconstruction speed and colorization quality.In this study,we report a generalized dual-color-space constrained model for FPM colorization.This model provides a mathematical framework for model-based FPM colorization,enabling a closed-form solution without the need for redundant iterative calculations.Our approach,termed generalized CFPM(gCFPM),achieves colorization within seconds for megapixel-scale images,delivering superior colorization quality in terms of both colorfulness and sharpness,along with an extended DOF.Both simulations and experiments demonstrate that gCFPM surpasses state-of-the-art methods across all evaluated criteria.Our work offers a robust and comprehensive workflow for high-throughput full-color pathological imaging using FPM platforms,laying a solid foundation for future advancements in methodology and engineering.
