Iron chalcogenides have attracted great interest as potential substitutes of nature enzymes in the colorimetric biological sensing due to their unique chemodynamic characteristics.Herein,we report the preparation of u...Iron chalcogenides have attracted great interest as potential substitutes of nature enzymes in the colorimetric biological sensing due to their unique chemodynamic characteristics.Herein,we report the preparation of ultrathin Fe S nanosheets(NSs)by a simple one-pot hydrothermal method and the prepared Fe S NSs exhibit strong Fenton-reaction activity to catalyze hydrogen peroxide(H_(2)O_(2))for generation of hydroxyl radical(^(·)OH).Based on the chromogenic reaction of resultant^(·)OH with 3,3,5,5-tetramethylbenzidine(TMB),we develop colorimetric biosensors for highly sensitive detection of H_(2)O_(2)and glutathione(GSH).The fabricated biosensors show wide linear ranges for the detection of H_(2)O_(2)(5–150μmol/L)and GSH(5–50μmol/L).Their detection limits for H_(2)O_(2)and GSH reach as low as0.19μmol/L and 0.14μmol/L,respectively.The experimental results of sensing intracellular H_(2)O_(2)and GSH demonstrate that this colorimetric method can realize the accurate detection of H_(2)O_(2)and GSH in normal cells(L02 and 3T3)and cancer cells(MCF-7 and He La).Our results have demonstrated that the synthesized Fe S NSs is a promising material to construct colorimetric biosensors for the sensitive detection of H_(2)O_(2)and GSH,holding great promising for medical diagnosis in cancer therapy.展开更多
Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne diseases.In this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and smartphone.First,m...Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne diseases.In this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and smartphone.First,micropump and microvalves were constructed by deforming the MRE under magnetic actuation and integrated into the microfluidic biosensor for fluidic control.Then,the micropump was used to deliver immune porous gold@platinum nanocatalysts(Au@PtNCs),bacterial sample,and immunomagnetic nanoparticles(MNPs)into a micromixer,where they were mixed,incubated and magnetically separated to obtain the Au@PtNC-bacteria-MNP complexes.After 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide were injected and catalyzed by the Au@PtNCs,smartphone was used to measure the color of the catalysate for quantitative analysis of target bacteria.Under optimal conditions,this biosensor could detect Salmonella typhimurium quantitatively and automatically in 1 h with a linear detection range of 8.0×10^(1) CFU/mL to 8.0×10^(4) CFU/mL and a detection limit of 62 CFU/mL.The microfluidic biosensor was compact in size,simple to use,and efficient for detection,and might be used for in-field screening of foodborne pathogens to prevent food poisoning.展开更多
基金the Key Grant for Special Professors in Jiangsu Province(No.RK030STP18001)the National Postdoctoral Program for Innovative Talents(No.BX20190156)+1 种基金the China Postdocoral Science Foundation funded project(No.2021M691654)the“1311 Talents Program”of Nanjing University of Posts and Telecommunications,the Scientific Research Foundation of Nanjing University of Posts and Telecommunications(Nos.NY218150,NY221042)。
文摘Iron chalcogenides have attracted great interest as potential substitutes of nature enzymes in the colorimetric biological sensing due to their unique chemodynamic characteristics.Herein,we report the preparation of ultrathin Fe S nanosheets(NSs)by a simple one-pot hydrothermal method and the prepared Fe S NSs exhibit strong Fenton-reaction activity to catalyze hydrogen peroxide(H_(2)O_(2))for generation of hydroxyl radical(^(·)OH).Based on the chromogenic reaction of resultant^(·)OH with 3,3,5,5-tetramethylbenzidine(TMB),we develop colorimetric biosensors for highly sensitive detection of H_(2)O_(2)and glutathione(GSH).The fabricated biosensors show wide linear ranges for the detection of H_(2)O_(2)(5–150μmol/L)and GSH(5–50μmol/L).Their detection limits for H_(2)O_(2)and GSH reach as low as0.19μmol/L and 0.14μmol/L,respectively.The experimental results of sensing intracellular H_(2)O_(2)and GSH demonstrate that this colorimetric method can realize the accurate detection of H_(2)O_(2)and GSH in normal cells(L02 and 3T3)and cancer cells(MCF-7 and He La).Our results have demonstrated that the synthesized Fe S NSs is a promising material to construct colorimetric biosensors for the sensitive detection of H_(2)O_(2)and GSH,holding great promising for medical diagnosis in cancer therapy.
文摘Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne diseases.In this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and smartphone.First,micropump and microvalves were constructed by deforming the MRE under magnetic actuation and integrated into the microfluidic biosensor for fluidic control.Then,the micropump was used to deliver immune porous gold@platinum nanocatalysts(Au@PtNCs),bacterial sample,and immunomagnetic nanoparticles(MNPs)into a micromixer,where they were mixed,incubated and magnetically separated to obtain the Au@PtNC-bacteria-MNP complexes.After 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide were injected and catalyzed by the Au@PtNCs,smartphone was used to measure the color of the catalysate for quantitative analysis of target bacteria.Under optimal conditions,this biosensor could detect Salmonella typhimurium quantitatively and automatically in 1 h with a linear detection range of 8.0×10^(1) CFU/mL to 8.0×10^(4) CFU/mL and a detection limit of 62 CFU/mL.The microfluidic biosensor was compact in size,simple to use,and efficient for detection,and might be used for in-field screening of foodborne pathogens to prevent food poisoning.
