Root rot in Panax notoginseng is primarily caused by Fusarium oxysporum.A rhizosphere-derived Trichoderma hamatum strain,Z32,was isolated and evaluated for its biocontrol potential.Dual culture assays showed that Z32 ...Root rot in Panax notoginseng is primarily caused by Fusarium oxysporum.A rhizosphere-derived Trichoderma hamatum strain,Z32,was isolated and evaluated for its biocontrol potential.Dual culture assays showed that Z32 inhibited F.oxysporum's growth by 62%,and microscopy revealed hyphal coiling,indicating competition-based antagonism.Genome sequencing produced a 42.69 Mb assembly with 10,837 predicted genes,including 451 carbohydrate-active enzyme(CAZyme)genes involved in cell wall degradation.Ten biosynthetic gene clusters related to antimicrobial metabolites were identified,and metabolomic profiling confirmed multiple antifungal and growth-promoting compounds.These findings reveal that T.hamatum Z32 suppresses soilborne pathogens through diverse mechanisms,providing a promising and environmentally friendly strategy for controlling root rot in P.notoginseng.展开更多
基金funded by The Sino-Vietnamese International Joint Laboratory for Characteristic&Cash Crops Green Development of Yunnan Province(202403AP140013)the National Key Research and Development(2021YFD1601003)Yunnan Provincial Joint Funds for Agriculture General Program(202301BD070001-198).
文摘Root rot in Panax notoginseng is primarily caused by Fusarium oxysporum.A rhizosphere-derived Trichoderma hamatum strain,Z32,was isolated and evaluated for its biocontrol potential.Dual culture assays showed that Z32 inhibited F.oxysporum's growth by 62%,and microscopy revealed hyphal coiling,indicating competition-based antagonism.Genome sequencing produced a 42.69 Mb assembly with 10,837 predicted genes,including 451 carbohydrate-active enzyme(CAZyme)genes involved in cell wall degradation.Ten biosynthetic gene clusters related to antimicrobial metabolites were identified,and metabolomic profiling confirmed multiple antifungal and growth-promoting compounds.These findings reveal that T.hamatum Z32 suppresses soilborne pathogens through diverse mechanisms,providing a promising and environmentally friendly strategy for controlling root rot in P.notoginseng.
