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Analysis and Reduction of Cogging Torque of Permanent Magnet Synchronous Wind Generators based on the Magnetic Field Modulation Mechanism
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作者 Zexing Li Chao Liu +2 位作者 Yiwen Fan Zixuan Zhang Jiakuan Xia 《CES Transactions on Electrical Machines and Systems》 EI CSCD 2024年第4期463-470,共8页
In this paper,a magnetic field modulation model considering the influence of phase angles is established for the analysis and weakening of the cogging torque of the permanent magnet synchronous wind generations.Compar... In this paper,a magnetic field modulation model considering the influence of phase angles is established for the analysis and weakening of the cogging torque of the permanent magnet synchronous wind generations.Compared with the existing analytical model,the modulation effect of the magnetic field harmonics and phase angle on the cogging torque components is analyzed in the new model.Firstly,flux density model with phase angle characteristics is derived,and the relationship of the cogging torque and magnetic field harmonic is analyzed using energy method.Then,based on the magnetic modulation mechanism,the impact of the phase angle and magnetic field harmonics on the coupling relationship among cogging torque components is analyzed.All cogging torque components are classified as a combination of multiple positive and negative harmonic components,and the contribution characteristics of the components are determined by the harmonic combination and phase angle characteristics.Based on the finite element model(FEM),the magnetic field modulation model of the cogging torque is proved.On the basis of the conclusions obtained,it is further explained that the suppression mechanism of rotor-step skewing is a mutual complementary effect of the positive components and negative cogging components,and the main harmonic is effectively offset by selecting the seasonable of segment number and skewed angle of rotor.Finally,in order to verify the validity of the analysis method,the no-load line back EMF and cogging torque of optimized prototype is tested,and the experimental results agree well with the FEM results. 展开更多
关键词 Magnetic field modulation model cogging torque Energy method
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基于PID与cogging力补偿的直线电动机控制 被引量:3
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作者 穆海华 周云飞 严思杰 《微电机》 北大核心 2007年第10期48-51,共4页
直线电动机cogging力是影响伺服控制定位精度和跟踪精度的重要因素。目前电动机几何结构优化与有效的控制策略是减小直线电动机cogging力的主要措施。在分析已有研究的基础上,提出了一种完全基于试验的cogging力迭代标定方法,并结合PID... 直线电动机cogging力是影响伺服控制定位精度和跟踪精度的重要因素。目前电动机几何结构优化与有效的控制策略是减小直线电动机cogging力的主要措施。在分析已有研究的基础上,提出了一种完全基于试验的cogging力迭代标定方法,并结合PID位置/力控制策略实现了直线电动机cogging力的补偿。所提出的方法已在气浮结构运动平台上进行了验证,给出了cogging力迭代标定的试验结果,对比了cogging力补偿前后的伺服控制跟踪误差。结果表明了提出方法的实用性和有效性。 展开更多
关键词 cogging 标定 补偿 PID控制 直线电动机
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Cogging Torque Reduction in Axial Flux PMSM with Different Permanent Magnet Combination 被引量:4
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作者 WANG Xiaoguang LIU Cheng +2 位作者 ZHOU Sheng WAN Ziwei LIU Yi 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2021年第5期726-736,共11页
With the increasing requirement for the mechanical vibration and acoustic noise of the permanent magnet synchronous motor(PMSM)drive system,the demand for cogging torque reduction of PMSM has been considerably increas... With the increasing requirement for the mechanical vibration and acoustic noise of the permanent magnet synchronous motor(PMSM)drive system,the demand for cogging torque reduction of PMSM has been considerably increased.To solve the problem of oversized cogging torque of axial flux PMSM,a rotor topology with hybrid permanent magnet is proposed to weaken the cogging torque.Firstly,the expression of the cogging torque of the axial flux motor is derived,and the influence of the pole-arc ratio of the permanent magnet on the cogging torque is analyzed.Secondly,the rotor structure with hybrid permanent magnet is adopted to reduce the cogging torque.According to the analytical analysis,the constraints of the size and pole-arc ratio between the hybrid permanent magnets are obtained,and the two permanent magnets related to the minimum cogging torque are determined.And the analysis results are verified by the finite element simulation.Furthermore,the motor performance with and without the hybrid permanent magnet is compared with each other.Finally,the cogging torque is significantly reduced by adopting a rotor structure with hybrid permanent magnets. 展开更多
关键词 axial flux permanent magnet synchronous motor(AFPMSM) rotor structure cogging torque hybrid permanent magnet pole-arc ratio
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Reduction of Cogging Torque and Electromagnetic Vibration Based on Different Combination of Pole Arc Coefficient for Interior Permanent Magnet Synchronous Machine 被引量:10
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作者 Feng Liu Xiuhe Wang +2 位作者 Zezhi Xing Aiguo Yu Changbin Li 《CES Transactions on Electrical Machines and Systems》 CSCD 2021年第4期291-300,共10页
Cogging torque and electromagnetic vibration are two important factors for evaluating permanent magnet synchronous machine(PMSM)and are key issues that must be considered and resolved in the design and manufacture of ... Cogging torque and electromagnetic vibration are two important factors for evaluating permanent magnet synchronous machine(PMSM)and are key issues that must be considered and resolved in the design and manufacture of high-performance PMSM for electric vehicles.A fast and accurate magnetic field calculation model for interior permanent magnet synchronous machine(IPMSM)is proposed in this article.Based on the traditional magnetic potential permeance method,the stator cogging effect and complex boundary conditions of the IPMSM can be fully considered in this model,so as to realize the rapid calculation of equivalent magnetomotive force(MMF),air gap permeance,and other key electromagnetic properties.In this article,a 6-pole 36-slot IPMSM is taken as an example to establish its equivalent solution model,thereby the cogging torque is accurately calculated.And the validity of this model is verified by a variety of different magnetic pole structures,pole slot combinations machines,and prototype experiments.In addition,the improvement measure of the machine with different combination of pole arc coefficient is also studied based on this model.Cogging torque and electromagnetic vibration can be effectively weakened.Combined with the finite element model and multi-physics coupling model,the electromagnetic characteristics and vibration performance of this machine are comprehensively compared and analyzed.The analysis results have well verified its effectiveness.It can be extended to other structures or types of PMSM and has very important practical value and research significance. 展开更多
关键词 cogging torque different combination of pole arc coefficient electromagnetic vibration interior permanent magnet synchronous machine
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Study on cogging force of permanent magnet linear synchronous motor
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作者 上官璇峰 袁世鹰 励庆孚 《Journal of Coal Science & Engineering(China)》 2005年第1期89-92,共4页
Presented the methods to obtain the cogging force of permanent magnet linear synchronous motors(PMLSMs), analyzed the characteristics of the cogging force, and provided a basis for reducing the effect of the cogging f... Presented the methods to obtain the cogging force of permanent magnet linear synchronous motors(PMLSMs), analyzed the characteristics of the cogging force, and provided a basis for reducing the effect of the cogging force. 2-dimensional finite element method(2D FEM) was used to solve the whole motor when its mover was at dif- ferent position, so that the relation was derived between the cogging force and the mover position. The analysis results show that the cogging force between the two ends of the primary iron-core and the secondary permanent magnets (PMs) is sinusoidal function of the mover position under certain conditions only. Two proposed methods, namely direct and indirect methods, are applied to calculate the cogging force between the primary iron-core teeth and the secondary PMs. The agreement of the two methods is validated. 展开更多
关键词 PMLSMs cogging force FEM
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Research on Cogging Force Suppression for Toroidal PMLSM Based on Disturbance Observer
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作者 Rongtao Zeng Jinghong Zhao +1 位作者 Sinian Yan Yinhao Mao 《CES Transactions on Electrical Machines and Systems》 CSCD 2023年第2期187-192,共6页
For a new type of toroidal permanent magnet linear motor(TPMLSM), this paper analyzes the thrust fluctuation in the constant acceleration operation of the motor from the Angle of the cogging force of the linear motor.... For a new type of toroidal permanent magnet linear motor(TPMLSM), this paper analyzes the thrust fluctuation in the constant acceleration operation of the motor from the Angle of the cogging force of the linear motor. For the motor whose structure has been determined and processed, the structural parameters of the motor cannot be changed, and its performance cannot be improved from the perspective of the motor body.Therefore, this paper tries to consider the influence of the cogging force on the normal operation of the motor from the perspective of control. In this paper, starting from the body structure of motor, first on the annular linear motor of the cogging force characteristics were extracted, and its expression is obtained by Fourier decomposition, then investigated considering the cogging force and does not consider the cogging force control of motor model, it can be seen that the control performance deteriorates significantly after considering cogging force of the motor, and the acceleration fluctuation increases significantly during the operation of the motor. On this basis, disturbance observation algorithm is introduced, and feedforward compensation is carried out by extracting the characteristic values of the disturbance model. The results show that the disturbance observer can suppress the thrust fluctuation caused by the motor cogging force to a large extent, and it can reduce the peak-to-peak value of the thrust fluctuation by more than 85% during the motor acceleration operation. 展开更多
关键词 TPMLSM cogging Force Perturbation Observer Thrust Fluctuation
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Influence of Design Parameters on Cogging Torque in Directly Driven Permanent Magnet Synchronous Wind Generators
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作者 Q.L. Deng S.D. Huang F. Xiao 《Journal of Energy and Power Engineering》 2010年第7期42-47,共6页
In order to reduce the cogging torque, this paper investigates the influence of some parameters on the cogging torque developed by directly driven permanent magnet synchronous wind generators. Based on the remanent ma... In order to reduce the cogging torque, this paper investigates the influence of some parameters on the cogging torque developed by directly driven permanent magnet synchronous wind generators. Based on the remanent magnetic flux densities, the cogging torque is computed by using finite element method. It is shown that many parameters have influence on cogging torque and the slot and pole number combination has a significant effect on cogging torque. A simple factor has been introduced to indicate the effect of the slot and pole number combination. Some practical experience to reduce the cogging torque was applied to 2 MW three phase permanent magnet synchronous generator at rated speed of 37.5 rpm for wind energy conversion. The simulation and experiment results verify the effect of the proposed method. 展开更多
关键词 cogging torque permanent magnet synchronous generator electric machine design.
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Investigation of Cogging Torque Generation Mechanisms in Flux-switching Permanent Magnet Machines Based on General Air-gap Field Modulation Theory
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作者 Ming Cheng Zhiyuan Xu +1 位作者 Yang Jiang Chenchen Zhao 《Chinese Journal of Electrical Engineering》 2025年第2期207-215,共9页
An analytical approach is proposed for the cogging torque of a flux-switching permanent magnet machine based on the general air-gap field modulation theory. The modulation process is first investigated, and the coggin... An analytical approach is proposed for the cogging torque of a flux-switching permanent magnet machine based on the general air-gap field modulation theory. The modulation process is first investigated, and the cogging torque generation mechanism is analyzed based on the distribution of the air-gap magnetic flux density harmonics. Thus, the relationship between the air-gap field harmonics and the cogging torque is revealed, and the contribution of each harmonic to the cogging torque is calculated using the proposed method. Simultaneously, the characteristics of the cogging torque, including amplitude and frequency, are analyzed. Subsequently, the calculated cogging torque is compared with the simulated torque using finite element analysis. The two results exhibit considerable consistency, confirming the feasibility of the proposed method. Moreover, a prototype experiment is conducted, and the cogging torque is measured to verify the effectiveness of the proposed method. 展开更多
关键词 Air-gap field cogging torque field modulation FLUX-SWITCHING permanent magnet
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A novel strategy for ingot cogging without homogenization:Dynamical recrystallization and nucleation mechanisms associated with as-cast dendrites of nickel-based superalloys
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作者 B.C.Xie Y.W.Luo +3 位作者 Z.T.Wang Q.Q.Meng Y.Q.Ning M.W.Fu 《Journal of Materials Science & Technology》 2025年第17期78-91,共14页
Since the as-cast microstructure benefits dynamic recrystallization(DRX)nucleation,the present research is focused on the microstructure evolution associated with the dendrites and precipitates during the thermal defo... Since the as-cast microstructure benefits dynamic recrystallization(DRX)nucleation,the present research is focused on the microstructure evolution associated with the dendrites and precipitates during the thermal deformation of an ingot without homogenization treatment aiming at exploring a new efficient strategy of ingot cogging for superalloys.The as-cast samples were deformed at the sub-solvus temperature,and the DRX evolution from dendritic arms(DAs)to inter-dendritic regions(IDRs)was discussed based on the observation of the fishnet-like DRX microstructures and the gradient of DRX grain size at IDRs.The difference in the precipitates at DAs and IDRs played an essential role during the deformation and DRX process,which finally resulted in very different microstructures in the two areas.A selective straininduced grain boundary bulging(SIGBB)mechanism was found to function well and dominate the DRX nucleation at DAs.The grain boundary was able to migrate and bulge to nucleate on the condition that the boundary was located at DAs and had a great difference in dislocation density between its opposite sides at the same time.As for DRX nucleation at IDRs,the particle-stimulated nucleation(PSN)mechanism played a leading role,and the progressive subgrain rotation(PSR)and geometric DRX were two important supplementary mechanisms.The dislocation accumulation around the coarse precipitates at IDR resulted in progressive orientation rotation,which would generate DRX nuclei once the maximum misorientation there was sufficient to form a high-angle boundary with the matrix.The PSR or geometric DRX functioned at the severely elongated IDRs at the later stage of deformation,depending on the thickness of the elongated IDRs.The uniform microstructure was obtained by the deformation without homogenization and the subsequent annealing treatment.The smaller strain,the lower annealing temperature,and the much shorter soaking time requested in the above process lead to a smaller risk of cracking and a lower consumption of energy during the ingot-cogging process. 展开更多
关键词 Superalloys Ingot cogging Dendrites Dynamic recrystallization Nucleation mechanisms
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山茱萸CoG10H1基因的克隆和表达分析
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作者 原梦 谭新杰 +7 位作者 邓萍 王盼盼 许家齐 李文文 李义龙 吕淑芳 王玉龙 侯典云 《中草药》 北大核心 2025年第11期4036-4042,共7页
目的克隆山茱萸Cornus officinalis G10H1基因(CoG10H1),并进行相关的生物信息学分析、表达分析和亚细胞定位研究。方法基于山茱萸转录组数据筛选并克隆CoG10H1基因,并对其CoG10H1及其编码蛋白的理化性质、二级结构、三级结构等进行分... 目的克隆山茱萸Cornus officinalis G10H1基因(CoG10H1),并进行相关的生物信息学分析、表达分析和亚细胞定位研究。方法基于山茱萸转录组数据筛选并克隆CoG10H1基因,并对其CoG10H1及其编码蛋白的理化性质、二级结构、三级结构等进行分析。利用实时荧光定量聚合酶链式反应PCR(real-time fluorescent quantitative polymerase chain reaction,qRT-PCR)对CoG10H1进行组织特异性表达分析。构建pCAMBIA1300-CoG10H1-GFP融合表达载体并侵染烟草叶片,激光共聚焦显微镜观察CoG10H1蛋白的亚细胞定位。结果CoG10H1的c DNA长度为1497 bp,编码499个氨基酸,有1个信号肽,在茎中表达量稍高,果实和叶片中次之,激光共聚焦显微镜下观察到CoG10H1蛋白定位于内质网中。结论CoG10H1蛋白属于细胞色素P450蛋白家族,可能在马钱苷合成通路中的发挥关键作用,为后续研究CoG10H1基因在马钱苷合成通路调控中的作用研究提供参考。 展开更多
关键词 山茱萸 CoG10H1 生物信息学 亚细胞定位 实时荧光定量PCR
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Influence of Magnet Shape on the Cogging Torque of a Surface-mounted Permanent Magnet Motor 被引量:15
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作者 Min Zhou Xinxing Zhang +2 位作者 Wenxiang Zhao Jinghua Ji Jingning Hu 《Chinese Journal of Electrical Engineering》 CSCD 2019年第4期40-50,共11页
The influence of bread-loaf shaped magnet poles under parallel magnetization on the cogging torque of surface-mounted permanent magnet(SPM)motors is studied.For the SPM motors having magnetic poles with eccentricity a... The influence of bread-loaf shaped magnet poles under parallel magnetization on the cogging torque of surface-mounted permanent magnet(SPM)motors is studied.For the SPM motors having magnetic poles with eccentricity and sine harmonic compensation,the electromagnetic performances of integer and fractional slot motors are compared.It is found that the cogging torque and torque ripple of the integer and fractional slot motors can be reduced with the same eccentric magnet pole.The cogging torque and torque ripple of a fractional slot motor can be decreased by sine harmonic compensation,however,the same sine harmonic compensation has a small influence in integer slot motors.By varying the magnetic poles,the contribution of the field harmonics(k=(2n+1)p),which are a direct result of magnet magnetization,to the cogging torque also changes.The electromagnetic performance of a 3 kW prototype is tested,and it is found that the experimental results validate the theoretical investigation. 展开更多
关键词 Analytical model bread-loaf magnet shape finite-element(FE) cogging torque surface-mounted permanent magnet(SPM)motor
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COG喷吹量对高炉冶炼过程影响数值模拟
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作者 周圣智 周密 +3 位作者 王文鹏 唐文博 王磊 厉英 《工程科学学报》 北大核心 2025年第11期2203-2212,共10页
基于国家“碳减排、碳中和”战略,向高炉中喷吹富氢气体成为有效减排的重要方法,其中关于高炉喷吹焦炉煤气(COG)的研究也得到了广泛关注,但针对COG喷吹量对高炉冶炼过程影响的相关研究相对较少.在此基础上,本文采用计算流体动力学数值... 基于国家“碳减排、碳中和”战略,向高炉中喷吹富氢气体成为有效减排的重要方法,其中关于高炉喷吹焦炉煤气(COG)的研究也得到了广泛关注,但针对COG喷吹量对高炉冶炼过程影响的相关研究相对较少.在此基础上,本文采用计算流体动力学数值模拟方法,探究COG喷吹量对高炉内煤粉燃烧率、气体还原反应、风口处焦炭反应及软熔带等因素的影响.结果表明,随着COG喷吹量由30 m^(3)·min^(-1)提升至120 m^(3)·min^(-1),H_(2)O、CO、CO_(2)、H_(2)等组分的含量表现出一定程度的上升趋势.从煤粉燃烧率的角度看,随着COG喷吹量的增加,煤粉的挥发反应基本相同,而水煤气反应得到增强.同时以氢气为主的还原路径以及水煤气反应得到强化,且炉内的软熔带位置以及形状发生改变,基于本次模拟,COG喷吹量达到120 m^(3)·min^(-1)时相对适宜,其软熔带的厚度有所减小,透气性得到改善,气速分布相对均匀. 展开更多
关键词 高炉 富氢气体 焦炉煤气(COG) 数值模拟 喷吹量
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The Characteristics Analysis and Cogging Torque Optimization of a Surface-Interior Permanent Magnet Synchronous Motor 被引量:3
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作者 Jikai Si Suzhen Zhao +2 位作者 Lufeng Zhang Ruiwu Cao Wenping Cao 《Chinese Journal of Electrical Engineering》 CSCD 2018年第4期41-47,共7页
This paper proposes optimal stator skewed slot analytical method for cogging torque reduction in surface-interior permanent magnet synchronous motor(SIPMSM)and analyzes the characteristics of SIPMSM.The series-paralle... This paper proposes optimal stator skewed slot analytical method for cogging torque reduction in surface-interior permanent magnet synchronous motor(SIPMSM)and analyzes the characteristics of SIPMSM.The series-parallel equivalent magnetic circuit models(EMCMs)of SIPMSM is built based on the characteristics of magnetic circuits,which is used to design the basic electromagnetic parameters of SIPMSM.Analytical expressions of cogging torque are derived from applying analytical techniques.Stator skewed slot for cogging torque minimum is adopted,and the stator skewed slot pitch is confirmed based on the analytical expressions of the resultant cogging torque.The cogging torque,torque ripple,back electromotive force(back-EMF),power-angle characteristics,efficiency and power factor of SIPMSM are analyzed by establishing 3-dimensional finite element model(3-D-FED)of SIPMSM with stator skewed slot and straight slot.It is shown that the comprehensive performance of optimized SIPMSM is improved as confirmed by finite element analysis and analytical calculation results. 展开更多
关键词 Surface-interior permanent magnet synchronous motor(SIPMSM) cogging torque stator skewed slot three-dimensional finite element model(3-D-FED)
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Winding Function Model-based Performance Evaluation of a PM Transverse Flux Generator for Applications in Direct-drive Systems 被引量:1
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作者 Mehrage Ghods Jawad Faiz Ali A Pourmoosa 《CES Transactions on Electrical Machines and Systems》 EI CSCD 2024年第2期216-226,共11页
The magnetic flux in a permanent magnet transverse flux generator(PMTFG) is three-dimensional(3D), therefore, its efficacy is evaluated using 3D magnetic field analysis. Although the 3D finite-element method(FEM) is h... The magnetic flux in a permanent magnet transverse flux generator(PMTFG) is three-dimensional(3D), therefore, its efficacy is evaluated using 3D magnetic field analysis. Although the 3D finite-element method(FEM) is highly accurate and reliable for machine simulation, it requires a long computation time, which is crucial when it is to be used in an iterative optimization process. Therefore, an alternative to 3DFEM is required as a rapid and accurate analytical technique. This paper presents an analytical model for PMTFG analysis using winding function method. To obtain the air gap MMF distribution, the excitation magneto-motive force(MMF) and the turn function are determined based on certain assumptions. The magnetizing inductance, flux density, and back-electro-magnetomotive force of the winding are then determined. To assess the accuracy of the proposed method, the analytically calculated parameters of the generator are compared to those obtained by a 3D-FEM. The presented method requires significantly shorter computation time than the 3D-FEM with comparable accuracy. 展开更多
关键词 Index Terms—Permanent magnet transverse flux generator Winding function 3D-FEM cogging torque PROTOTYPING
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张宣科技氢基竖炉提质降耗的措施
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作者 王海涛 《炼铁》 CAS 北大核心 2024年第5期72-76,共5页
张宣科技氢基竖炉投产初期,COG单耗较高,DRI金属化率不稳定、含碳量较高、抗压强度偏低,储运和使用过程中DRI产生粉末较多,且由于DRI结构疏松、气孔率高,易发生再氧化。通过采取提升球团矿质量、适当提高竖炉进口温度、合理调控还原循... 张宣科技氢基竖炉投产初期,COG单耗较高,DRI金属化率不稳定、含碳量较高、抗压强度偏低,储运和使用过程中DRI产生粉末较多,且由于DRI结构疏松、气孔率高,易发生再氧化。通过采取提升球团矿质量、适当提高竖炉进口温度、合理调控还原循环气体成分及钝化储存等措施,提质降耗取得显著成效。2023年10月-2024年2月,氢基竖炉平均COG单耗降低166m^(3)/t,矿比降低至1368kg/t,DRI金属化率提高0.59个百分点,一级品率明显提高;DRI含碳量稳定,抗压强度超过800N/个球,脱氧泥产生量降低6.1kg/t,含粉率降低2.43个百分点。 展开更多
关键词 氢基竖炉 DRI COG单耗 球团矿 还原制度
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基于MongoDB GridFS的遥感影像管理平台设计与开发
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作者 赵海鹏 《测绘与空间地理信息》 2024年第9期120-123,共4页
针对多源、多时相、多分辨率遥感影像的数据存储形式单一、管理和应用手段比较落后等问题,设计了一套基于MongoDB GridFS的海量遥感影像管理发布服务平台,使用GDAL对遥感影像进行数据处理和自动化混合切片、MongoDB存储影像切片数据和... 针对多源、多时相、多分辨率遥感影像的数据存储形式单一、管理和应用手段比较落后等问题,设计了一套基于MongoDB GridFS的海量遥感影像管理发布服务平台,使用GDAL对遥感影像进行数据处理和自动化混合切片、MongoDB存储影像切片数据和影像元数据、Mongo GridFS存储原始影像、HTTP文件服务器托管COG,构建了以原始影像-元数据-切片-COG为组织结构的遥感影像管理发布服务平台的数据基底,实现了遥感影像在线查询和预览、数据入库和分发等服务,为遥感影像在自然资源监测、生态环境保护、国土空间规划等领域的应用提供了有力支持。 展开更多
关键词 遥感影像管理 影像服务 COG MongoDB GridFS
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大垫尖翅蝗转录组分析 被引量:20
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作者 金永玲 丛斌 +2 位作者 王丽艳 张海燕 董辉 《昆虫学报》 CAS CSCD 北大核心 2015年第8期817-825,共9页
【目的】大垫尖翅蝗Epacromius coerulipes(Ivanov)是广泛分布的草原蝗虫之一,但其基因资源缺乏。为了获得大垫尖翅蝗的基因数据,对其进行了转录组测序和分析。【方法】利用Illumina公司paired-end转录组的测序技术进行从头组装。【结... 【目的】大垫尖翅蝗Epacromius coerulipes(Ivanov)是广泛分布的草原蝗虫之一,但其基因资源缺乏。为了获得大垫尖翅蝗的基因数据,对其进行了转录组测序和分析。【方法】利用Illumina公司paired-end转录组的测序技术进行从头组装。【结果】总计获得了63 033条unigenes,平均长度为772 bp,N50为1 589 bp。通过BLAST搜索,确定有25 132条(39.87%)unigenes与NCBI数据库已知的蛋白质相匹配,其中有24 841,16 490,11 558和8 013条unigenes成功注释到Nr,Swiss-Prot,GO和COG数据库中。KEGG数据库中,7 218条unigenes形成218条代谢或信息通路。其中,189条unigenes参与外源性物质或药物的代谢通路。进一步分析显示,213条unigenes被确认为可能参与外源性物质的解毒作用,29条unigenes被确定为编码杀虫剂的目标蛋白。此外,检测到5 696条简单重复序列。【结论】该转录组测序分析将为进一步研究大垫尖翅蝗的基因功能分析及杀虫剂的抗药性机制分析奠定分子基础。 展开更多
关键词 大垫尖翅蝗 转录组 基因注释 GO数据库 COG数据库 KEGG数据库 简单重复序列
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擎天凤梨花器官全长cDNA文库的构建及EST分析 被引量:11
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作者 刘建新 沈福泉 +5 位作者 田丹青 张智 葛亚英 沈晓岚 俞信英 王炜勇 《分子植物育种》 CAS CSCD 2009年第6期1137-1143,共7页
为了通过基因工程手段来培育花色丰富、花型奇特的擎天凤梨新品种,从擎天凤梨中筛选出花色、花型相关调控基因则是十分必要的。本研究以擎天凤梨属品种Ostara为材料,采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库,初始文... 为了通过基因工程手段来培育花色丰富、花型奇特的擎天凤梨新品种,从擎天凤梨中筛选出花色、花型相关调控基因则是十分必要的。本研究以擎天凤梨属品种Ostara为材料,采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库,初始文库滴度为3×106,插入片段平均长度大于1kb;随机挑取2004个阳性克隆进行5'EST测序,获得高质量序列1758条,经拼接获得1365条单基因簇(unigene),其中跨叠群(contig)175个和单条序列(singlet)1195个;经ORF寻找共获得有效ORF1283条;经COG分析EST序列编码的蛋白质被分为22类;经Blast分析后,获得一批花色、花器官发育、花期调控以及其它育种价值基因(包括cDNA全长或片段)。该研究结果为擎天凤梨的花色、花型转基因等育种奠定了基础。 展开更多
关键词 擎天凤梨 CDNA文库 SMART EST COG
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茶树品种福鼎大白茶和小雪芽叶片基因转录组研究 被引量:8
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作者 李娜娜 陆建良 +1 位作者 郑新强 梁月荣 《江苏农业学报》 CSCD 北大核心 2012年第5期974-978,共5页
以茶树品种福鼎大白茶和低温诱导型新梢白化茶树品种小雪芽叶片为材料,构建基因转录本;组装得到79 797条茶树参考基因库(Unigene),其中,基因序列长度在1 000 bp以上的有11 371条,占Unigene库总数的14.25%。SSR分析显示,共有6 439条SSR,... 以茶树品种福鼎大白茶和低温诱导型新梢白化茶树品种小雪芽叶片为材料,构建基因转录本;组装得到79 797条茶树参考基因库(Unigene),其中,基因序列长度在1 000 bp以上的有11 371条,占Unigene库总数的14.25%。SSR分析显示,共有6 439条SSR,其中1~3个碱基的完美重复SSR占84.32%。基因表达丰度RPKM值显示,小雪芽表达上调的基因数为1 821个,表达下调基因数为1 779个。差异表达基因GO和COG分析显示,引起低温诱导型茶树新梢白化的可能遗传机理存在于蛋白质翻译后的修饰过程,而且这些变异主要发生在细胞内。 展开更多
关键词 转录组 新梢白化 茶树参考基因库 SSR 基因表达 GO COG
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脑卒中患者下肢外骨骼康复机器人步态规划与运动学仿真 被引量:10
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作者 朱志伟 唐春霞 +1 位作者 徐立娟 陈殿生 《机械设计与制造》 北大核心 2022年第1期247-250,256,共5页
为满足越来越多的脑卒中患者辅助行走和康复训练的需要,设计了一款下肢外骨骼机器人模型,采用D-H参数法建立踝关节、膝关节、髋关节坐标系,推演出步态周期内的坐标方程。为了安全起见,要求脑卒中患者步行速度慢且步长短,利用CoG(Center ... 为满足越来越多的脑卒中患者辅助行走和康复训练的需要,设计了一款下肢外骨骼机器人模型,采用D-H参数法建立踝关节、膝关节、髋关节坐标系,推演出步态周期内的坐标方程。为了安全起见,要求脑卒中患者步行速度慢且步长短,利用CoG(Center of Gravity,重心地面投影点)作为步态规划中的稳定性判断依据,并用Robotics Toolbox for Matlab仿真,结果表明:下肢外骨骼康复机器人在康复训练过程中各关节具有连续且稳定的步态轨迹,为后续脑卒中患者使用的下肢外骨骼康复机器人样机研制提供了必要的理论依据。 展开更多
关键词 脑卒中 步态规划 COG 下肢外骨骼机器人 MATLAB
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